Comparison of serum protein profile in Indonesian Local Ettawah goats with single and twin offspring using SDS-PAGE

Abstract

Indonesian Local Ettawah Goats (ILEG) is local Indonesian livestock with more than one offspring potential. There is no description of the serum protein profile of female goat with single and twin offspring. This study aimed to compare the protein band type, the percentage of protein band appearance, protein band thickness between the female goat serum of single and twin offspring. This research method was a case study at the breeding village of Ampel Gading Malang East Java, Indonesia. The sample came from ILEG female with single and twin offspring, which had a record of three offsprings with six replications per group. Serum samples were isolated from whole blood taken through the goat jugular vein. Separation of blood serum with Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and performed by Panther Data Base for analysis of protein type data. The results showed that single and twin ILEG had ten types of protein bands 13-140 kDa with an average percentage of protein band appearances of 8.32% higher in twin offsprings compared to single offspring. The thickening level of a protein band at 44-94 kDa in female goat with twin offsprings was increased expression compared to single offspring. The ILEG protein profile of twin offspring was higher in quality and quantity than single offspring. The research recommends molecular protein weight at 44-94 kDa as a candidate to an early detect female goat with twin offspring.