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Detection and Quantification of Anti-oxidant markers like Rutin, Catechin, Quercetin, Gallic acid, Ellagic acid, Ferulic acid, Vitexin and Mangiferin in Herbal raw materials available in market belongs to Rutaceae family by HPTLC Technique

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Ramasamy Arivukkarasu*, Aiyalu Rajasekaran

KMCH College of Pharmacy, Coimbatore, Tamilnadu, India.

*Corresponding Author E-mail: [email protected]

ABSTRACT:

The primary aim of the study is to detect the flavonoids, phenolic acids and xanthones in three commercial herbal raw materials namely Aegle marmelos, Citrus aurantifolia and Murraya koenigii obtained from Rutaceae family used in daily domestic needs to confirm the presence of mentioned common secondary metabolites in herbal raw materials. Results of the study clearly revealed that these raw materials from rutaceae family contains flavonoids, phenolic acids and xanthones. The developed HPTLC method can be employed for the routine investigations of flavonoids, phenolic acids and xanthones in herbal raw materials. Aegle marmelos, Citrus aurantifolia and Murraya koenigii was ascertain the presence of Rutin and shows the 0.072%,0.005%0.002% respectively. Ellagic acid was originate to be not more than 0.004% each of Aegle marmelos and Citrus aurantifolia. Quercetin was found to 0.034% .062% 0.021% respectively in all test three extracts. Vitexin was found to be 0.019% in Citrus aurantifolia. Murraya koenigii revels presence of 0.002%, 0.001% of mangiferin and catechin respectively

KEYWORDS: Rutin, Quercetin, Catechin, Mangiferin, HPTLC.

INTRODUCTION:

The modern scientific community has presently recognized flavonoids to be a unique class of therapeutic molecules due to their diverse therapeutic properties. Standardization of herbal formulation in terms of raw materials, manufacturing practices and composition is important to ensure quality and optimum level of active principles for their bio potency1.

The primary aim of the study is to detect the flavonoids, phenolic acids and xanthones in three commercial herbal raw materials obtained from Rutaceae family in daily domestic needs as antioxidants, antidiabetic, antipyretic, ulcer healing, diuretic, anti-fertility and anti-inflammatory agent and dysentery, constipation and diarrhea, relieves morning sickness, nausea, eliminates bacteria, heals wounds, burns and Reduces stress. Rutin (3,3′,4′,5,7-pentahydroxyflavone-3-rhamnoglucoside) is a flavonol, abundantly found in plants, Citrus leaves contain rutin at concentrations...