Abstract

Doc number: 35

Abstract

Background: Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear.

Results: Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC50 of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study.

Conclusion: Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway.

Details

Title
In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell
Author
Chong, Hueh Zan; Rahmat, Asmah; Yeap, Swee Keong; Md Akim, Abdah; Alitheen, Noorjahan Banu; Othman, Fauziah; Gwendoline-Ee, Cheng Lian
Pages
35
Publication year
2012
Publication date
2012
Publisher
BioMed Central
e-ISSN
1472-6882
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1472-6882-12-35
ProQuest document ID
1020835536
Copyright
© 2012 Chong et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.