Abstract

Doc number: 375

Abstract

Background: Variations in cytokine and immune mediator expression patterns in amniotic fluid due to gestational age, maternal age and fetal gender were investigated.

Findings: Amniotic fluid samples were obtained from 192 women, 82 with a mid-trimester amniocentesis (median gestational age 17 weeks) and 110 with a caesarean section not in labor (median gestational age 39 weeks). Amniotic fluid was screened by commercial ELISAs for the TH1/TH2/TH17 cytokines and immune mediators IL-1 beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-15, IL-17, TNF alpha, GRO-alpha, MIP1alpha, MIP1beta, Histone, and IP10. Analysis was by Bonferroni correction for multiple comparisons. None of the 15 examined cytokines revealed any differences in expression patterns regarding fetal gender. Significant differences were found in IL-4, IL-10, IL-12, TNF- alpha, GRO-alpha and MIP1-beta with respect to gestational age and in GRO-alpha regarding maternal age.

Conclusion: Cytokines utilized as biomarkers in the diagnosis of intrauterine infections are not influenced in their expression pattern by fetal gender but may vary with respect to maternal age and gestational age.

Details

Title
Influence of maternal age, gestational age and fetal gender on expression of immune mediators in amniotic fluid
Author
Weissenbacher, Tobias; Laubender, Rüdiger P; Witkin, Steven S; Gingelmaier, Andrea; Schiessl, Barbara; Kainer, Franziskus; Friese, Klaus; Jeschke, Udo; Dian, Darius; Karl, Katrin
Pages
375
Publication year
2012
Publication date
2012
Publisher
BioMed Central
e-ISSN
17560500
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1114720698
Copyright
© 2012 Weissenbacher et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.