Full text

Turn on search term navigation

© 2011 Ganesan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Ganesan LP, Mohanty S, Kim J, Clark KR, Robinson JM, et al. (2011) Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium. PLoS Pathog 7(9): e1002281. doi:10.1371/journal.ppat.1002281

Abstract

The liver removes quickly the great bulk of virus circulating in blood, leaving only a small fraction to infect the host, in a manner characteristic of each virus. The scavenger cells of the liver sinusoids are implicated, but the mechanism is entirely unknown. Here we show, borrowing a mouse model of adenovirus clearance, that nearly all infused adenovirus is cleared by the liver sinusoidal endothelial cell (LSEC). Using refined immunofluorescence microscopy techniques for distinguishing macrophages and endothelial cells in fixed liver, and identifying virus by two distinct physicochemical methods, we localized adenovirus 1 minute after infusion mainly to the LSEC (~90%), finding ~10% with Kupffer cells (KC) and none with hepatocytes. Electron microscopy confirmed our results. In contrast with much prior work claiming the main scavenger to be the KC, our results locate the clearance mechanism to the LSEC and identify this cell as a key site of antiviral activity.

Details

Title
Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium
Author
Ganesan, Latha P; Mohanty, Sudhasri; Kim, Jonghan; Clark, K Reed; Robinson, John M; Anderson, Clark L
Section
Research Article
Publication year
2011
Publication date
Sep 2011
Publisher
Public Library of Science
ISSN
15537366
e-ISSN
15537374
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1289107218
Copyright
© 2011 Ganesan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Ganesan LP, Mohanty S, Kim J, Clark KR, Robinson JM, et al. (2011) Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium. PLoS Pathog 7(9): e1002281. doi:10.1371/journal.ppat.1002281