Impaired Auditory-Vestibular Functions and Behavioral Abnormalities of Slitrk6-Deficient Mice

Abstract

A recent study revealed that Slitrk6, a transmembrane protein containing a leucine-rich repeat domain, has a critical role in the development of the inner ear neural circuit. However, it is still unknown how the absence of Slitrk6 affects auditory and vestibular functions. In addition, the role of Slitrk6 in regions of the central nervous system, including the dorsal thalamus, has not been addressed. To understand the physiological role of Slitrk6, Slitrk6-knockout (KO) mice were subjected to systematic behavioral analyses including auditory and vestibular function tests. Compared to wild-type mice, the auditory brainstem response (ABR) of Slitrk6-KO mice indicated a mid-frequency range (8–16 kHz) hearing loss and reduction of the first ABR wave. The auditory startle response was also reduced. A vestibulo-ocular reflex (VOR) test showed decreased vertical (head movement–induced) VOR gains and normal horizontal VOR. In an open field test, locomotor activity was reduced; the tendency to be in the center region was increased, but only in the first 5 min of the test, indicating altered adaptive responses to a novel environment. Altered adaptive responses were also found in a hole-board test in which head-dip behavior was increased and advanced. Aside from these abnormalities, no clear abnormalities were noted in the mood, anxiety, learning, spatial memory, or fear memory–related behavioral tests. These results indicate that the Slitrk6-KO mouse can serve as a model of hereditary sensorineural deafness. Furthermore, the altered responses of Slitrk6-KO mice to the novel environment suggest a role of Slitrk6 in some cognitive functions.

About the Authors:

Yoshifumi Matsumoto

Affiliation: Laboratory for Behavioral and Developmental Disorders, RIKEN Brain Science Institute (BSI), Wako-shi, Japan

Kei-ichi Katayama

Affiliation: Laboratory for Behavioral and Developmental Disorders, RIKEN Brain Science Institute (BSI), Wako-shi, Japan

Takehito Okamoto

Affiliation: Laboratory for Motor Learning Control, RIKEN Brain Science Institute (BSI), Wako-shi, Japan

Kazuyuki Yamada

Affiliation: Support Unit for Animal Experiments, RIKEN Brain Science Institute (BSI), Wako-shi, Japan

Noriko Takashima

Affiliation: Laboratory for Behavioral and Developmental Disorders, RIKEN Brain Science Institute (BSI), Wako-shi, Japan

Soichi Nagao

Affiliation: Laboratory for Motor Learning Control, RIKEN Brain Science Institute (BSI), Wako-shi, Japan

Jun Aruga

* E-mail: [email protected]

Affiliation: Laboratory for Behavioral and Developmental Disorders, RIKEN Brain Science Institute (BSI), Wako-shi, Japan

Introduction

Slitrk6 belongs to the Slitrk family of transmembrane proteins with neurite outgrowth modulating activities [1], [2]. Structurally, Slitrk members share two leucine-rich repeat domains located amino-terminal to the transmembrane domain. In the carboxy-terminus, there are conserved tyrosine residues flanked by amino acid sequences similar to those in the carboxy-terminal domain of the Ntrk neurotrophin receptor [3]. Among the six Slitrk family genes (Slitrk1–6) in mice, Slitrk6 shows a unique expression pattern, with strong expression in the inner ear and modest expression in the dorsal thalamus at both embryonic and postnatal stages [1], [4]–[6].

A recent study revealed that Slitrk6 promotes innervation and survival of inner ear sensory neurons in part by modulating neurotrophin–Ntrk signaling [5]. Slitrk6-knockout (KO) mice showed reduced cochlear innervation. In the vestibule, the innervation to the posterior crista was often lost, reduced, or sometimes misguided. These defects were accompanied by the loss of neurons in the spiral and vestibular ganglia. This study addressed only the embryonic to early postnatal morphological phenotype of Slitrk6-KO mice, and analyses of any functional deficits caused by the developmental defects of the inner ear remain to be conducted.

Previous studies reported Slitrk family members as candidate genes controlling several neuropsychiatric disorders [7]–[13]. Considering the expression of Slitrk6 in the mature central nervous system [1], [2] (http://www.brain-map.org/), it is possible that Slitrk6 has a role in the expression of higher brain functions.

In the present study, we evaluated the neurological characteristics of the Slitrk6-KO mouse by performing systematic behavioral analyses and physiological tests related to inner ear functions. The results revealed deficits in the auditory, vestibular, and cognitive functions of Slitrk6-KO mice. We discussed the biological significance of Slitrk6 and the similarities of deficits in the KO mice to the phenotypes of some neurological diseases.

Results

General features of the Slitrk6-KO mouse

The Slitrk6 null allele (Slitrk6−) was generated by replacing its entire protein-coding region by a loxP sequence [5]. Both male and female Slitrk6-KO (Slitrk6−/−) mice grew without showing any external abnormalities and were fertile [5]. A slight, but significant reduction in body weight was observed in the adult male mice (wild type [WT]: n = 10, 26.5±0.57 g [mean ± SE]; KO: n = 10, 24.6±0.19 g at 12 weeks old). However, the reason for this weight reduction is not clear. We used adult male Slitrk6-KO mice and WT (Slitrk6+/+) littermates for the behavioral and inner ear function tests. The behavioral tests are listed in Table 1.

[Figure omitted. See PDF.]

Table 1. Summary of Slitrk6 KO behavioral analyses.

https://doi.org/10.1371/journal.pone.0016497.t001

Auditory function defects in Slitrk6-KO mice

Auditory brain-stem response (ABR) is an electrical signal reflecting the neuronal activities related to auditory information processing. The ABR method has been shown to be effective for assessing auditory function in mice [14]. We recorded ABRs in anesthetized mice with ear and scalp electrodes. The responses were recorded upon delivering sound stimuli with certain ranges of frequencies (2–24 kHz) and strength (10–80 dB). Figure 1A presents the ABR waves from WT and Slitrk6-KO mice. To determine the hearing function of Slitrk6-KO mice, we first measured the thresholds for the appearance of a recognizable wave representing ABR (Fig. 1B). The results indicated significant increments (ca. 20 dB) of the sound intensity threshold for 8-kHz (WT  = 48.5±2.6 [mean ± SE], KO  = 62.2±2.22; P<0.01, Mann-Whitney's U-test) and 16-kHz (WT  = 17.1±1.84, KO  = 30±2.88; P<0.05) stimuli, but not for 2-, 4-, and 24-kHz stimuli at 4 weeks of age. When the amplitude and latency values of peaks I, II, and III with an 80-dB stimulus were compared between WT and Slitrk6-KO mice (Fig. 1C top), there was a significant decrement in the peak I absolute values of 16-kHz (WT  = 5.42±0.43 [mean ± SE], KO  = 3.55±0.31; P = 0.0031), 8-kHz (WT  = 2.51±0.15, KO  = 1.36±0.16; P = 0.00020), and 4-kHz (WT  = 2.58±0.09, KO  = 2.02±0.16; P = 0.012) stimuli-induced responses in Slitrk6-KO mice (Fig. 1C middle). There also was a significant increment in the peak III absolute values for the 24-kHz stimulus (Fig. 1C middle; WT  = 0.69±0.34, KO  = 1.76±0.20; P = 0.015). However, the peak latency was not significantly different between the two genotypes (Fig. 1C bottom).

[Figure omitted. See PDF.]

Figure 1. Auditory function abnormalities in Slitrk6-KO mice.

(A) Representative waves of auditory brainstem response (ABR) from WT (left, n = 7) and Slitrk6-KO (right, n = 9) mice. ABR were recorded upon 0- to 80-dB sound pressure level (SPL) stimuli of 2, 4, 8, 16, and 24 kHz. The dash lines indicate the time of click presentation. The scale bars are shown at bottom right. (B) ABR thresholds in WT and Slitrk6-KO mice. Slitrk6-KO mice showed significantly higher thresholds to the 8- and 16-kHz stimuli than those of WT mice. (C) Top: Each peak number in a representative ABR wave. Middle: Comparison of the values of peaks I, II, and III between WT and Slitrk6-KO mice. Peak I of Slitrk6-KO mice was significantly reduced in the range of 8 to 16 kHz, and peak III was also significantly reduced at 24 kHz. Bottom: The latency to peaks I, II, and III. The latency did not show clear differences between WT and Slitrk6-KO mice. (D) Auditory startle response of WT (n = 10) and Slitrk6-KO mice (n = 10). Slitrk6-KO mice showed significantly lower startle responses to 95- to 120-dB sounds. *P<0.05, **P<0.01. All bars are mean ± standard error of the mean.

https://doi.org/10.1371/journal.pone.0016497.g001

Auditory startle response (ASR) was included in the auditory function analysis. We measured the ASR following white noise stimulation with various sound intensities (Fig. 1D; 70–120 dB on 65-dB background noise). Compared with WT mice, Slitrk6-KO mice showed significant decrements of ASR toward the stronger sound stimuli (95–120 dB; F1,18 = 9.7, P = 0.0059, two-way ANOVA, main effect of genotype) but not toward the weaker ones (70–90 dB). While there was a clear difference in ASR between the genotypes, there were no significant differences in the prepulse (70, 75, and 80 dB) inhibition of ASR induced by a 120-dB stimulus (Table 1). Thus, both ABR and ASR results indicated that there was an auditory function deficit in the Slitrk6-KO mice.

Vertical vestibular function defects in Slitrk6-KO mice

A previous study revealed the frequent absence of vestibular nerve projection to the posterior crista in Slitrk6-KO mice [5]. Therefore, functions of the semicircular canal were investigated. We measured eye movements induced by rotation of the head on the plane of the horizontal semicircular canal, that is, the horizontal vestibulo-ocular reflex (hVOR), or on the plane perpendicular to the plane of the horizontal semicircular canal, the vertical vestibulo-ocular reflex (vVOR). The horizontal optokinetic response (hOKR), which shares the same neural circuit within the cerebellum as that of the hVOR, was measured as a reference. There were no clear differences in the hVOR or hOKR gains and phases between WT and Slitrk6-KO mice (Fig. 2A, B). For the vVOR, however, Slitrk6-KO mice showed significantly decreased gains at all four frequencies examined (Fig. 2C; F1,12 = 11.7, P = 0.0051, two-way ANOVA, main effect of genotype). There were no significant differences in the vVOR phases. These results indicated that there was a functional deficit in the semicircular canal of Slitrk6-KO mice.

[Figure omitted. See PDF.]

Figure 2. Vestibular function anomaly in Slitrk6-KO mice.

(A) Dynamic characteristics of horizontal optokinetic response (hOKR) of WT (n = 9) and Slitrk6-KO (n = 9) mice. (B) Horizontal vestibulo-ocular reflex (hVOR). There were no differences in gains (left) or phases (right) between WT (n = 9) and KO (n = 9) in hVOR or hOKR. (C) Vertical vestibulo-ocular reflex (vVOR) of WT (n = 8) and Slitrk6-KO mice (n = 6). Gains of vVOR (left) of the Slitrk6-KO mice were significantly smaller than those of WT. (D) Rota- rod test. Rotation indicates the speed of rotation (rpm) at which the mice fell off or revolved around the rod. The values were comparable between WT (n = 12) and Slitrk6-KO (n = 8) mice, suggesting that there were no strong deficits of balancing function in Slitrk6-KO mice. Values are mean ± standard error of the mean.

https://doi.org/10.1371/journal.pone.0016497.g002

We also performed behavioral tests related to vestibular functions, such as swimming, contact righting, and suspension [15]. In the suspension test, we did not see the trunk curling posture that is typically found in mice with impaired vestibular function [15] (data not shown). Swimming and contact righting did not show clear differences between the two genotypes (data not shown). In addition, Slitrk6-KO mice displayed a comparable performance to that of WT mice in the rota-rod test (Fig. 2D), which partly reflect vestibular function. These results suggested that the vestibular functional deficit in Slitrk6-KO mice is mild and selective for the posterior semicircular canal system.

Adaptive responses to environmental change are altered in Slitrk6-KO mice

In addition to inner ear–related abnormalities in Slitrk6-KO mice, novel phenotypes became clear based on their performance in a battery of behavioral tests (Table 1). First, we noticed altered activities of Slitrk6-KO mice in the home cage (Fig. 3). The activity level during the early dark phase tended to be lower (20:00–01:00, P = 0.11; 23:00–01:00, P = 0.068) and that during the beginning of the light phase was significantly higher (08:00–09:00, P = 0.045) than activity levels of the WT mice.

[Figure omitted. See PDF.]

Figure 3. Spontaneous activity in home cage.

Each vertical bar represents standard error of the mean. #P = 0.068; *P<0.05. WT, n = 10; KO, n = 10. Below the graph, open and black bars indicate light phase (08:00–20:00) and dark phase (20:00–08:00), respectively.

https://doi.org/10.1371/journal.pone.0016497.g003

In an open field (OF) test with a 15-min observation period, the Slitrk6-KO mice showed less locomotor activity (Fig. 4A), although the difference was limited to the first 5 min (Fig. 4A; P = 0.0023). Furthermore, the percentage of time the Slitrk6-KO mice remained in the center area was significantly longer than the WT only in the first 5 min (Fig. 4B; P = 0.0082, Mann-Whitney's U-test). The abnormalities only in the early phase were thought to reflect some altered adaptive responses to the novel environment.

[Figure omitted. See PDF.]

Figure 4. Altered adaptive responses to a novel environment in Slitrk6-KO mice.

(A, B) Open field test. (A) The total distance moved was less in Slitrk6-KO than in WT mice (left). The distance moved in the first 5 min of the test was significantly lower in Slitrk6-KO mice (right). (B) In Slitrk6-KO mice, the total time spent in the center area was greater than that of the WT (left) and was significantly increased in the first 5 min of the test (right). (C–G) Hole-board test. There were no significant differences in distance moved (C), number of rearing episodes (D), and number of head dips (E) between Slitrk6-KO and WT mice. Duration per head dip was significantly decreased in Slitrk6-KO mice (F). Latency time to head dip was significantly decreased in KO mice (G). Values are mean ± standard error of the mean. *P<0.05, **P<0.01 for Mann-Whitney's U-test (B, center area data) or Student's t-test (others). WT, n = 10; KO, n = 10.

https://doi.org/10.1371/journal.pone.0016497.g004

We also noted altered responses to a novel environment in the hole-board (HB) test, which was carried out for 5 min in a box that was the same size as the OF box. However, the HB box differed from the OF box in terms of color, brightness, and the presence of four holes on its floor. The total duration of head dipping into the holes by Slitrk6-KO mice was more than twice that of WT (Fig. 4D; P = 0.046). In addition, the latency to the first head dip was significantly shorter than that in the WT (Fig. 4E; P = 0.028).

Because Slitrk6-KO mice showed altered spontaneous activities in the three different contexts (home cage, OF box, and HB box), we evaluated whether these behavioral abnormalities were caused by elevated anxiety in a novel environment. We therefore performed the elevated plus maze test and light–dark box test, which are commonly used in neurobiological anxiety research. In these tests, however, we did not find any obvious differences between WT and Slitrk6-KO mice (Fig. 5). These results indicated that alterations of spontaneous activity in the home cage, OF, and HB tests are due to cognitive dysfunction in the Slitrk6-KO mice, rather than elevated anxiety.

[Figure omitted. See PDF.]

Figure 5. Normal anxiety level of Slitrk6-KO mice.

https://doi.org/10.1371/journal.pone.0016497.g005

The Morris water maze test, fear-conditioning test, forced swimming test, social interaction tests, tail flick test, and hot plate test did not show any abnormalities in the Slitrk6-KO mice as compared to the WT (Table 1).

Discussion

The Slitrk6-KO mouse is a novel animal model of sensorineural deafness

ABRs and ASRs indicated that there is an impaired auditory function in Slitrk6-KO mice. A mild (ca. 20-dB) elevation of the sound intensity threshold was observed for 8- and 16-kHz sound stimuli, resulting in a strong decrement of the ABR first wave (peak I). The results suggest that the reduction of peak I to the 80-dB stimulus is related to both the Slitrk6 loss-of-function and the impaired auditory function. Although the precise origins of ABR waves are not yet well defined, it is generally agreed that the first wave represents activities of the auditory nerve and the late waves represent neural transmission within the central auditory circuit [16]. Our previous study revealed that during embryonic development of Slitrk6-KO mice the number of axon projection from the spiral ganglion to cochlear hair and the number of the spiral ganglion neurons were lower than seen in WT mice [5]. The total cell number in the spiral ganglion of Slitrk6-KO mice was one half that in WT, and the decreased number of the projection was clearly observed as late as 4 weeks after birth. Therefore, it is likely that the reduction of peak I may reflect these morphological differences in the inner ear neural circuit of Slitrk6-KO mice.

It is interesting that elevation of the sound intensity threshold was not observed for the 24-kHz sound stimulus. The frequency-selective sensitivity loss might not be fully explained by the innervation defects in the inner ear, considering that the projection impairment was generally observed from the basal to apical region of the cochlea. It is possible that some mechanism compensates for the desensitization to 24-kHz sounds. The enigmatic peak III increment might reflect such a process.

Many genes are associated with hearing impairment in mice [17], [18], and 44 of them have been linked to human hereditary hearing loss [17]. Our findings indicate that Slitrk6-KO mice could be a novel model of sensorineural deafness. According to the current hereditary hearing loss database (http://hereditaryhearingloss.org), the nearest neighboring gene related to hearing impairment is an autosomal dominant nonsyndromic gene (DFNA 33, 13q34-qter) [19] approximately 27 Mb apart from SLITRK6 (13q31.2). Thus, the involvement of functional deficits in SLITRK6 in human hereditary hearing impairment awaits further investigation. Furthermore, Slitrk6 inner ear abnormalities are known to involve altered neurotrophin-signaling [5]. NT-3 delivery has been attempted in model animals [20]–[22] with the aim of clinical treatment of hearing impairment, and it would be beneficial to further clarify the mechanism of Slitrk6 action in development of the inner ear and auditory signal processing.

Vestibular function abnormalities in Slitrk6-KO mice

Slitrk6-KO mice showed moderate deficit in the gain of vVOR, but not in hVOR. Based on the orientations of mouse semicircular canals [23], horizontal semicircular canals are selectively stimulated in the test of hVOR, whereas the anterior and posterior semicircular canals are equally stimulated in the test of vVOR in our assay system. The involvement of otolith in the head rotation used in our vVOR test was small, as the effect of gravity was minimal in the head rotation in nose-up position perpendicular to the earth axis. On the other hand, we previously showed that the neural projection to posterior crista, which develops to posterior semicircular canal, was often lost in Slitrk6-KO mice (78.3% at E13.5 [n = 83], 85.7% at E15.5 [n = 28], and 90.3% after P14 [n = 62]), but those to the anterior and horizontal canal cristae and otolith organs remained intact [5]. Thus, the defect of VOR (normal hVOR and impaired vVOR) is consistent with the anomaly of vestibular nerve projection (intact innervation of anterior and horizontal cristae and decreased innervation of posterior crista) in Slitrk6-KO mice. Concurrently, the VOR results suggest that function of Slitrk6-KO horizontal canal is not obviously impaired, indistinguishable from those of WT. However, the functional and structural properties of the remained inner ear neural circuits of Slitrk6-KO should be more carefully examined because embryonic Slitrk6 expression broadly occurs in the inner ear sensory epithelia [5].

Many mutant mice with vestibular function abnormalities are listed in the current mouse genome informatics database (http://www.informatics.jax.org/). However, we did not find any mice with vertical-direction limited VOR abnormalities. In this regard, the vestibular dysfunction in Slitrk6-KO mice is unique. Together with the selective innervation defects to the posterior crista, Slitrk6-KO mice could serve as a useful model to study the role of the semicircular canal in vestibular function.

Although the vestibular function alteration was clear in the vVOR, it is unclear how the other behavioral phenotypes in Slitrk6-KO mice reflect the altered vestibular function. For example, Slitrk6-KO mice showed a significant decrement in the immobility time of the tail suspension test (Table 1). This alteration could indicate the absence of a normal vestibular response for vertical head movement. However, even if this is the case, the overall vestibular function deficit appears to be mild and selective. The vestibular innervation defects in the posterior canal may be partially compensated by the anterior semicircular canal and possibly the otolith organs, which also sense vertical head movements.

In humans, a posterior semicircular canal dysfunction is related to benign paroxysmal positional vertigo, in which displaced otoconia are thought to enter a semicircular canal, usually in the posterior one [24], [25]. Although this is a disorder of late onset (mean onset 54 years old) [24], there are pediatric patients with balance and vestibular disorders [26], [27]. Considering that significant associations exist between sensorineural hearing loss and balance disorders [26], it is possible that SLITRK6 is involved in some pathophysiological processes of pediatric vestibular disorders.

Cognitive dysfunction of Slitrk6-KO mice

This study revealed novel behavioral phenotypes of Slitrk6-KO mice (Table 1). Among them, the abnormalities revealed by the OF and HB tests may reflect similar aspects of altered higher brain function in Slitrk6-KO mice. In both tests, the abnormalities appeared soon (within 5 min) after entry into the test boxes. Therefore, we assume that the novel environments induced the differential behaviors. Our findings clearly indicated a neurological basis for these behavioral abnormalities, which appeared to reflect increased attention to the environment and emotional responses similar to “restlessness” or “confusion.”

It is tempting to correlate these behavioral abnormalities with the role of Slirk6 in the thalamus, where its expression is clearly demarcated among regions of the central nervous system [1], [4], [6]. If we assume that the thalamic expression is significant, some sensory information processing during adaptive behaviors may be dysfunctional in Slitrk6-KO mice, considering the many roles played by the thalamus in relaying and modulating sensory signals [28]–[30]. Future studies should examine whether Slitrk6 loss-of-function impairs the development or the function of the thalamic neural circuit. Clarification of the molecular function of Slitrk6 will contribute to a better understanding of the higher function of the mammalian brain.

Materials and Methods

Animals

Animal experiments were approved by the Animal Experiment Committee of the RIKEN Brain Science Institute (approval no. H18-2B032), and the mice were maintained at the institute's Research Resource Center. The mice were kept on a 12-h light–dark cycle, with the dark cycle occurring from 20:00 to 08:00. All the testing described here was performed with adult male mice. The behavioral tests were started when the mice were 12 weeks old and completed before they reached the age of 16 weeks. In total, 45 pairs of WT and KO mice were used in this study.

Auditory brainstem response

For the measurement of ABRs, mice were anesthetized with an intraperitoneal injection of 60 mg/kg sodium pentobarbital (Nembutal, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan), and needle electrodes were inserted at the vertex and pinna with a ground near the tail. ABRs were evoked with 4-ms tone pips at 40 per second with a 0.4-ms cosine squared rise–fall envelope and alternating in polarity to remove frequency-following responses. The voltage difference between the pinna and vertex was amplified (10,000×), filtered, digitized at 100 kHz, and averaged across 512 presentations. The sound level was decreased in 10-dB steps from an 80-dB sound pressure level. The threshold, amplitude, and latency of responses were defined by visual inspection of stacked waveforms.

Auditory startle response

In this test, each mouse was put into a small cage (30 or 35 mm diameter, 12 cm long) that was set on a sensor block within a sound-proof chamber (60×50×67 cm [height]). A dim light was equipped on the ceiling of the sound-proof chamber (10 lux at the center of the sensor block), and a 65-dB white noise was presented as background noise. In the ASR test, each mouse was acclimatized to the experimental condition for 5 min, then the experimental session began. In the first session, a 120-dB startle stimulus (40 ms) was presented to the mouse 10 times at random intertrial intervals (10–20 s). In the second session, the startle response to stimuli at various intensities was assessed. Five times of 70 to 120 dB (70, 75, 80, 85, 90, 95, 100, 110, 120 dB) white noise stimuli (40 ms) were presented in quasi-random order and random inter-trial intervals (10–20 sec). In the prepulse inhibition session, the mouse experienced five types of trials: (1) no stimulus; (2) startle stimulus (120 dB, 40 ms) only; (3) 70-dB prepulse (20 ms, lead time 100 ms) and 120-dB pulse; (4) 75-dB prepulse (20 ms, lead time 100 ms) and120-dB pulse; and (5) 80-dB prepulse (20 ms, lead time 100 ms) and 120-dB pulse. Each trial was repeated 10 times in quasi-random order at random intertrial intervals (10–20 s). In the final session, again a 120-dB startle stimulus (40 ms) was presented to the mouse 10 times at random intertrial intervals (10–20 s). The total duration of an ASR test was about 35 to 40 min. After each trial, holding chambers were washed with tap water, wiped with a paper towel, and dried. Apparatuses and software for data analysis used were commercially available ones (Mouse Startle, O'Hara, Tokyo, Japan).

Vestibulo-ocular reflex and optokinetic response eye movements

Eye movement was measured by the infrared TV method as described previously [31], [32]. Under isofluorane (Escain, Mylan-Japan, Tokyo, Japan) anesthesia and aseptic conditions, a platform for head fixation was made on the mouse cranial bone using synthetic resin (Superbond C &B, Sun Medical, Tokyo, Japan) and one 15-mm stainless bolt. Two days after surgery, a mouse was mounted on the turntable surrounded by a checked-pattern (check size, 4°) screen (diameter, 60 cm; height, 60 cm), with the head fixed and the body loosely restrained in a plastic cylinder. The horizontal and vertical vestibulo-ocular reflexes (hVOR and vVOR, respectively) and horizontal optokinetic response (hOKR) eye movements were measured. The hVOR was tested in the dark by sinusoidal turntable oscillation at a frequency of 0.11–0.50 Hz and peak-to-peak amplitude of 10° on the plane parallel to the bilateral horizontal semicircular canals. The hOKR was tested in the light with sinusoidal screen oscillation at a frequency of 0.11–0.33 Hz and peak-to-peak amplitude of 10–20° (maximum screen velocity, 3.5–10.5°/s) on the same plane. The vVOR was tested in the dark with turntable oscillation at a frequency of 0.11–0.5 Hz and peak-to-peak amplitude of 10° on the plane perpendicular to the plane of the bilateral horizontal semicircular canals. During the test of vVOR, the mouse laid on its back in the plastic cylinder with the head-up position on the turntable [33]. More than 10 cycles of the evoked horizontal (hVOR and hOKR) or vertical (vVOR) eye movements free from artifacts due to blinks and saccades were averaged, and the mean amplitude and phase were calculated by a modified Fourier analysis [34]. A gain of the eye movement was defined as the ratio of the peak-to-peak amplitude of eye movements to that of the turntable or screen oscillation. The phase was defined as 0° when the peak of the eye movement was opposite to the peak of turntable oscillation in the hVOR and vVOR and when the peak of the eye movement matched the screen oscillation in the hOKR.

Rota-rod test

A mouse was placed on a rotating rod (O'Hara) and the time it was able to maintain its balance walking on top of the rod was measured. The speed of rotation was 4 rpm on day 1, and the speed was accelerated from 4 to 40 rpm over a 4-min period and then maintained at 40 rpm for another 1 min on days 2 to 5. Mice were tested in one trial for 2 min on day 1 and in four trials with a maximum time of 300 s (intertrial intervals were 20–30 s) on days 2 to 5. The time between placement and falling off or revolving around the rod was recorded manually.

Home cage activity

Spontaneous activity of each mouse in its home cage was measured using a 24-channel ABsystem 4.0 (Neuroscience, Tokyo, Japan). Cages were individually set into the stainless steel compartments of a negative breeding rack (JCL, Tokyo, Japan). An infrared sensor was equipped on the ceiling of each compartment and it detected movements of the mice. Home cage activity was measured for 1 week, starting from the afternoon of the day mice were transferred to the behavioral laboratory (day 1). Only data from days 2 to 8 were statistically analyzed. After termination of the home cage activity measurements, cages and bedding materials were changed and the mice were maintained in a micro-isolation rack (Allentown, PA, USA) throughout the behavioral screening.

Hole-board test

For this test, an OF system made of gray plastic (50×50×40 cm [height]) with four equally separated holes (3 cm diameter, each with an infrared sensor) on the floor was used (model ST-1/WII, Muromachi-kikai, Tokyo, Japan). The field was illuminated by fluorescent light (180 lux at the center of the field), and the level of background noise was approximately 50 dB. The behavior of each mouse was monitored by a CCD camera (placed about 1.5 m above the field). In the HB test, a mouse was introduced into the center of the field and allowed to explore freely for 5 min. Total moving time (s), distance traveled (cm), latency for head dipping (s), number of head dips, duration of head dipping (s), duration of rearing (s), and number of rearing episodes were measured as indices. Data were collected and analyzed using CompACT VAS system (Muromachi-kikai).

Open field, elevated plus maze, and light–dark box tests

Open field, elevated plus maze, and light–dark box tests were performed as described previously [35].

Statistical analyses

Statistical analyses were conducted using the SPSS statistical package (ver. 16.0, SPSS Japan Inc., Tokyo, Japan). Parametric data were analyzed by Student's t-test, and nonparametric data were analyzed by Mann-Whitney's U-test. Reported P values refer to Student's t-test unless otherwise noted. Effects of factors were analyzed by one-way ANOVA, two-way ANOVA with post hoc tests, and generalized linear model. Differences were defined as statistically significant when P<0.05.

Acknowledgments

We thank Hiromichi Goto (RIKEN Brain Science Institute) for teaching us how to perform the ABR measurement, and Azel Zine (University of Montpellier I) for valuable discussions.

Author Contributions

Conceived and designed the experiments: YM KK SN JA. Performed the experiments: YM KK TO KY NT SN. Analyzed the data: YM KK TO KY NT SN JA. Wrote the paper: YM KK KY SN JA.

Citation: Matsumoto Y, Katayama K-i, Okamoto T, Yamada K, Takashima N, Nagao S, et al. (2011) Impaired Auditory-Vestibular Functions and Behavioral Abnormalities of Slitrk6-Deficient Mice. PLoS ONE6(1): e16497. https://doi.org/10.1371/journal.pone.0016497

References

1. Aruga J, Mikoshiba K (2003) Identification and characterization of Slitrk, a novel neuronal transmembrane protein family controlling neurite outgrowth. Mol Cell Neurosci 24: 117–129.J. ArugaK. Mikoshiba2003Identification and characterization of Slitrk, a novel neuronal transmembrane protein family controlling neurite outgrowth.Mol Cell Neurosci24117129

2. Aruga J, Yokota N, Mikoshiba K (2003) Human SLITRK family genes: genomic organization and expression profiling in normal brain and brain tumor tissue. Gene 315: 87–94.J. ArugaN. YokotaK. Mikoshiba2003Human SLITRK family genes: genomic organization and expression profiling in normal brain and brain tumor tissue.Gene3158794

3. Patapoutian A, Reichardt LF (2001) Trk receptors: mediators of neurotrophin action. Curr Opin Neurobiol 11: 272–280.A. PatapoutianLF Reichardt2001Trk receptors: mediators of neurotrophin action.Curr Opin Neurobiol11272280

4. Aruga J (2003) Slitrk6 expression profile in the mouse embryo and its relationship to that of Nlrr3. Gene Expr Patterns 3: 727–733.J. Aruga2003Slitrk6 expression profile in the mouse embryo and its relationship to that of Nlrr3.Gene Expr Patterns3727733

5. Katayama K, Zine A, Ota M, Matsumoto Y, Inoue T, et al. (2009) Disorganized innervation and neuronal loss in the inner ear of Slitrk6-deficient mice. PLoS One 4: e7786.K. KatayamaA. ZineM. OtaY. MatsumotoT. Inoue2009Disorganized innervation and neuronal loss in the inner ear of Slitrk6-deficient mice.PLoS One4e7786

6. Stillman AA, Krsnik Z, Sun J, Rasin MR, State MW, et al. (2009) Developmentally regulated and evolutionarily conserved expression of SLITRK1 in brain circuits implicated in Tourette syndrome. J Comp Neurol 513: 21–37.AA StillmanZ. KrsnikJ. SunMR RasinMW State2009Developmentally regulated and evolutionarily conserved expression of SLITRK1 in brain circuits implicated in Tourette syndrome.J Comp Neurol5132137

7. Abelson JF, Kwan KY, O'Roak BJ, Baek DY, Stillman AA, et al. (2005) Sequence variants in SLITRK1 are associated with Tourette's syndrome. Science 310: 317–320.JF AbelsonKY KwanBJ O'RoakDY BaekAA Stillman2005Sequence variants in SLITRK1 are associated with Tourette's syndrome.Science310317320

8. Shmelkov SV, Hormigo A, Jing D, Proenca CC, Bath KG, et al. (2010) Slitrk5 deficiency impairs corticostriatal circuitry and leads to obsessive-compulsive-like behaviors in mice. Nat Med 16: 598–602.SV ShmelkovA. HormigoD. JingCC ProencaKG Bath2010Slitrk5 deficiency impairs corticostriatal circuitry and leads to obsessive-compulsive-like behaviors in mice.Nat Med16598602

9. Piton A, Gauthier J, Hamdan FF, Lafreniere RG, Yang Y, et al. Systematic resequencing of X-chromosome synaptic genes in autism spectrum disorder and schizophrenia. Mol Psychiatry. In press. A. PitonJ. GauthierFF HamdanRG LafreniereY. YangSystematic resequencing of X-chromosome synaptic genes in autism spectrum disorder and schizophrenia.Mol Psychiatry. In press

10. Smith EN, Bloss CS, Badner JA, Barrett T, Belmonte PL, et al. (2009) Genome-wide association study of bipolar disorder in European American and African American individuals. Mol Psychiatry 14: 755–763.EN SmithCS BlossJA BadnerT. BarrettPL Belmonte2009Genome-wide association study of bipolar disorder in European American and African American individuals.Mol Psychiatry14755763

11. Zuchner S, Cuccaro ML, Tran-Viet KN, Cope H, Krishnan RR, et al. (2006) SLITRK1 mutations in trichotillomania. Mol Psychiatry 11: 887–889.S. ZuchnerML CuccaroKN Tran-VietH. CopeRR Krishnan2006SLITRK1 mutations in trichotillomania.Mol Psychiatry11887889

12. Miranda DM, Wigg K, Kabia EM, Feng Y, Sandor P, et al. (2009) Association of SLITRK1 to Gilles de la Tourette syndrome. Am J Med Genet B Neuropsychiatr Genet 150B: 483–486.DM MirandaK. WiggEM KabiaY. FengP. Sandor2009Association of SLITRK1 to Gilles de la Tourette syndrome.Am J Med Genet B Neuropsychiatr Genet150B483486

13. Katayama K, Yamada K, Ornthanalai VG, Inoue T, Ota M, et al. (2010) Slitrk1-deficient mice display elevated anxiety-like behavior and noradrenergic abnormalities. Mol Psychiatry 15: 177–184.K. KatayamaK. YamadaVG OrnthanalaiT. InoueM. Ota2010Slitrk1-deficient mice display elevated anxiety-like behavior and noradrenergic abnormalities.Mol Psychiatry15177184

14. Willott JF (2006) Overview of methods for assessing the mouse auditory system. Curr Protoc Neurosci Chapter 8: Unit8 21A.JF Willott2006Overview of methods for assessing the mouse auditory system.Curr Protoc Neurosci Chapter8Unit8 21A

15. Hardisty-Hughes RE, Parker A, Brown SD (2010) A hearing and vestibular phenotyping pipeline to identify mouse mutants with hearing impairment. Nat Protoc 5: 177–190.RE Hardisty-HughesA. ParkerSD Brown2010A hearing and vestibular phenotyping pipeline to identify mouse mutants with hearing impairment.Nat Protoc5177190

16. Zhou X, Jen PH, Seburn KL, Frankel WN, Zheng QY (2006) Auditory brainstem responses in 10 inbred strains of mice. Brain Res 1091: 16–26.X. ZhouPH JenKL SeburnWN FrankelQY Zheng2006Auditory brainstem responses in 10 inbred strains of mice.Brain Res10911626

17. Friedman LM, Dror AA, Avraham KB (2007) Mouse models to study inner ear development and hereditary hearing loss. Int J Dev Biol 51: 609–631.LM FriedmanAA DrorKB Avraham2007Mouse models to study inner ear development and hereditary hearing loss.Int J Dev Biol51609631

18. Leibovici M, Safieddine S, Petit C (2008) Mouse models for human hereditary deafness. Curr Top Dev Biol 84: 385–429.M. LeiboviciS. SafieddineC. Petit2008Mouse models for human hereditary deafness.Curr Top Dev Biol84385429

19. Bonsch D, Schmidt CM, Scheer P, Bohlender J, Neumann C, et al. (2009) A new gene locus for an autosomal-dominant non-syndromic hearing impairment (DFNA 33) is situated on chromosome 13q34-qter. (German) HNO 57: 371–376.D. BonschCM SchmidtP. ScheerJ. BohlenderC. Neumann2009A new gene locus for an autosomal-dominant non-syndromic hearing impairment (DFNA 33) is situated on chromosome 13q34-qter.(German) HNO57371376

20. Carnicero E, Knipper M, Tan J, Alonso MT, Schimmang T (2002) Herpes simplex virus type 1-mediated transfer of neurotrophin-3 stimulates survival of chicken auditory sensory neurons. Neurosci Lett 321: 149–152.E. CarniceroM. KnipperJ. TanMT AlonsoT. Schimmang2002Herpes simplex virus type 1-mediated transfer of neurotrophin-3 stimulates survival of chicken auditory sensory neurons.Neurosci Lett321149152

21. Noushi F, Richardson RT, Hardman J, Clark G, O'Leary S (2005) Delivery of neurotrophin-3 to the cochlea using alginate beads. Otol Neurotol 26: 528–533.F. NoushiRT RichardsonJ. HardmanG. ClarkS. O'Leary2005Delivery of neurotrophin-3 to the cochlea using alginate beads.Otol Neurotol26528533

22. Staecker H, Kopke R, Malgrange B, Lefebvre P, Van de Water TR (1996) NT-3 and/or BDNF therapy prevents loss of auditory neurons following loss of hair cells. Neuroreport 7: 889–894.H. StaeckerR. KopkeB. MalgrangeP. LefebvreTR Van de Water1996NT-3 and/or BDNF therapy prevents loss of auditory neurons following loss of hair cells.Neuroreport7889894

23. Calabrese DR, Hullar , TE (2006) Planar relationships of the semicircular canals in two strains of mice. J Assoc Res Otolaryngol 7: 151–159.DR CalabreseHullarTE2006Planar relationships of the semicircular canals in two strains of mice.J Assoc Res Otolaryngol7151159

24. Salvinelli F, Firrisi L, Casale M, Trivelli M, D'Ascanio L, et al. (2004) Benign paroxysmal positional vertigo: diagnosis and treatment. Clin Ter 155: 395–400.F. SalvinelliL. FirrisiM. CasaleM. TrivelliL. D'Ascanio2004Benign paroxysmal positional vertigo: diagnosis and treatment.Clin Ter155395400

25. Korres SG, Balatsouras DG, Papouliakos S, Ferekidis E (2007) Benign paroxysmal positional vertigo and its management. Med Sci Monit 13: CR275–282.SG KorresDG BalatsourasS. PapouliakosE. Ferekidis2007Benign paroxysmal positional vertigo and its management.Med Sci Monit13CR275282

26. O'Reilly RC, Morlet T, Nicholas BD, Josephson G, Horlbeck D, et al. Prevalence of vestibular and balance disorders in children. Otol Neurotol. In press. RC O'ReillyT. MorletBD NicholasG. JosephsonD. HorlbeckPrevalence of vestibular and balance disorders in children.Otol Neurotol. In press

27. Wiener-Vacher SR (2008) Vestibular disorders in children. Int J Audiol 47: 578–583.SR Wiener-Vacher2008Vestibular disorders in children.Int J Audiol47578583

28. Butler AB (1995) The dorsal thalamus of jawed vertebrates: a comparative viewpoint. Brain Behav Evol 46: 209–223.AB Butler1995The dorsal thalamus of jawed vertebrates: a comparative viewpoint.Brain Behav Evol46209223

29. Nunez A, Malmierca E (2007) Corticofugal modulation of sensory information. Adv Anat Embryol Cell Biol 187: 1–74.A. NunezE. Malmierca2007Corticofugal modulation of sensory information.Adv Anat Embryol Cell Biol187174

30. Alitto HJ, Usrey WM (2003) Corticothalamic feedback and sensory processing. Curr Opin Neurobiol 13: 440–445.HJ AlittoWM Usrey2003Corticothalamic feedback and sensory processing.Curr Opin Neurobiol13440445

31. Katoh A, Kitazawa H, Itohara S, Nagao S (1998) Dynamic characteristics and adaptability of mouse vestibulo-ocular and optokinetic response eye movements and the role of the flocculo-olivary system revealed by chemical lesions. Proc Natl Acad Sci USA 95: 7705–7710.A. KatohH. KitazawaS. ItoharaS. Nagao1998Dynamic characteristics and adaptability of mouse vestibulo-ocular and optokinetic response eye movements and the role of the flocculo-olivary system revealed by chemical lesions.Proc Natl Acad Sci USA9577057710

32. Shutoh F, Ohki M, Kitazawa H, Itohara S, Nagao S (2006) Memory trace of motor learning shifts transsynaptically from cerebellar cortex to nuclei for consolidation. Neuroscience 139: 767–777.F. ShutohM. OhkiH. KitazawaS. ItoharaS. Nagao2006Memory trace of motor learning shifts transsynaptically from cerebellar cortex to nuclei for consolidation.Neuroscience139767777

33. Harrod CG, Baker JF (2003) The vestibulo ocular reflex (VOR) in otoconia deficient head tilt (het) mutant mice versus wild type C57BL/6 mice. Brain Res 972: 75–83.CG HarrodJF Baker2003The vestibulo ocular reflex (VOR) in otoconia deficient head tilt (het) mutant mice versus wild type C57BL/6 mice.Brain Res9727583

34. Jastreboff PW (1979) Evaluation and statistical judgment of neural responses to sinusoidal stimulation in cases with superimposed drift and noise. Biol Cybern 33: 113–120.PW Jastreboff1979Evaluation and statistical judgment of neural responses to sinusoidal stimulation in cases with superimposed drift and noise.Biol Cybern33113120

35. Sakatani S, Yamada K, Homma C, Munesue S, Yamamoto Y, et al. (2009) Deletion of RAGE causes hyperactivity and increased sensitivity to auditory stimuli in mice. PLoS One 4: e8309.S. SakataniK. YamadaC. HommaS. MunesueY. Yamamoto2009Deletion of RAGE causes hyperactivity and increased sensitivity to auditory stimuli in mice.PLoS One4e8309