High Resolution Structure of the ba3 Cytochrome c Oxidase from Thermus thermophilus in a Lipidic Environment

Abstract

The fundamental chemistry underpinning aerobic life on Earth involves reduction of dioxygen to water with concomitant proton translocation. This process is catalyzed by members of the heme-copper oxidase (HCO) superfamily. Despite the availability of crystal structures for all types of HCO, the mode of action for this enzyme is not understood at the atomic level, namely how vectorial H+ and e- transport are coupled. Toward addressing this problem, we report wild type and A120F mutant structures of the ba3-type cytochrome c oxidase from Thermus thermophilus at 1.8 Å resolution. The enzyme has been crystallized from the lipidic cubic phase, which mimics the biological membrane environment. The structures reveal 20 ordered lipid molecules that occupy binding sites on the protein surface or mediate crystal packing interfaces. The interior of the protein encloses 53 water molecules, including 3 trapped in the designated K-path of proton transfer and 8 in a cluster seen also in A-type enzymes that likely functions in egress of product water and proton translocation. The hydrophobic O2-uptake channel, connecting the active site to the lipid bilayer, contains a single water molecule nearest the CuB atom but otherwise exhibits no residual electron density. The active site contains strong electron density for a pair of bonded atoms bridging the heme Fea3 and CuB atoms that is best modeled as peroxide. The structure of ba3-oxidase reveals new information about the positioning of the enzyme within the membrane and the nature of its interactions with lipid molecules. The atomic resolution details provide insight into the mechanisms of electron transfer, oxygen diffusion into the active site, reduction of oxygen to water, and pumping of protons across the membrane. The development of a robust system for production of ba3-oxidase crystals diffracting to high resolution, together with an established expression system for generating mutants, opens the door for systematic structure-function studies.

About the Authors:

Theresa Tiefenbrunn

Contributed equally to this work with: Theresa Tiefenbrunn, Wei Liu

Affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America

Wei Liu

Contributed equally to this work with: Theresa Tiefenbrunn, Wei Liu

Affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America

Ying Chen

Affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America

Vsevolod Katritch

Affiliation: Skaggs School of Pharmacy & Pharmaceutical Sciences and San Diego Supercomputer Center, University of California, San Diego, La Jolla, California, United States of America

C. David Stout

Affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America

James A. Fee

* E-mail: [email protected] (VC); [email protected] (JF)

Affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America

Vadim Cherezov

* E-mail: [email protected] (VC); [email protected] (JF)

Affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America

Introduction

Heme/copper oxidases (HCO) represent a superfamily of enzymes found in the aerobic respiratory chain of mitochondria and bacteria that convert oxygen to water and transfer protons across membranes to form an electrochemical gradient. Members of this family include cytochrome c and quinol oxidases that have been phylogenetically grouped into three major subfamilies. The A-type is represented by some of the most studied cytochromes aa3 from bovine heart mitochondria [1]–[3] and from two bacteria, Paracoccus denitrificans (Pd) [4], [5] and Rhodobacter sphaeroides (Rs) [6], [7]. B-type enzymes, of which the prototypical member is the ba3-oxidase from Thermus thermophilus (Tt) [8], [9], are found in both eubacteria and archaea. The C-type oxidases (cbb3), found in two bacterial groups, are expressed at low oxygen levels, a feature they share with some pathogenic bacteria [10]. While highly divergent in amino acid sequence, HCOs of the different types share common structural elements, suggesting a similar mechanism of action.

Despite decades of investigation and the availability of structures for all HCO types [1]–[10], the details of oxygen reduction and proton transfer are not fully understood. In particular, while computational modeling has recently provided insights into the chemical mechanism of proton pumping in ba3 oxidase, atomic-level, experimental details of the coupling of proton transfer to the chemical steps of O2 reduction remain unclear [11]. Similarly, detailed mechanisms of the gating of proton channels to ensure unidirectional proton flow have yet to be elucidated [12]. Finally, questions remain regarding the path for the transfer of O2 from the membrane into the heme a3-CuB dinuclear center, as well as the nature of the inter-metal O atom(s), observed in the current and previous studies, between Fea3 and CuB [13], [14].

The ba3 cytochrome c oxidase from T. thermophilus represents a unique HCO system to study these mechanisms. The non-overlapping optical absorption spectra of the b-heme and the a3-heme centers permit their electronation states to be readily distinguished and quantified [15], [16]. Its low amino acid sequence similarity [17] to the well-studied aa3 oxidases quickly reveals those residues that are evolutionarily conserved and play essential roles in structure and mechanism and those that do not. Additionally, an expression system for ba3 has been developed, allowing for the straightforward generation of mutant enzymes [18], which have already been used to probe details of proton transfer [12], [19] and electron transfer [16] mechanisms.

The crystal structure of ba3-oxidase solubilized in detergent micelles was previously obtained at 2.4 Å [8] and 2.3 Å [9] resolution; however, while ba3 readily crystallizes in these systems, typically fewer than one crystal out of 30 diffracts better than ∼3 Å, which severely diminishes the possibilities for combined structure-function work at the single crystal level. Toward overcoming this barrier, we applied crystallization in lipidic cubic phase (LCP), also known as in meso crystallization [20], [21], and obtained highly reproducible crystals of ba3 oxidase diffracting to 1.7–1.8 Å. Initial success of in meso crystallization was related to obtaining high resolution structures of microbial rhodopsins [22]–[27] and recently to revealing several structures of human G protein-coupled receptors [28]–[31]. Apart from paving the way for more reproducible and higher resolution structures, this method also permits lipid content to be manipulated during crystallization trials and specific lipid-protein interactions to be observed [32].

In this work, we expand our understanding of the ba3-oxidase system along seven lines of enquiry: (1) use of lipidic matrix for HCO crystallization, (2) increasing the diffraction limit from 2.3 Å to 1.8 Å, (3) discovering and mapping evolutionarily conserved lipid binding sites similar to those previously described for A-type enzymes, (4) discovery of a novel lipid binding site extending ∼10 Å out of the plane of the membrane, (5) assignment of a well-resolved Fea3-O-O-CuB atomic arrangement indicative of a bound peroxo dianion, (6) new evidence that the large, hydrophobic O2-channel is devoid of all but one ordered water, and (7) characterization of a water cluster starting from a single conserved water molecule that bridges the propionate side-chains of heme-a3 and expanding to include 8 water molecules that lie at the interface of subunit I and subunit II.

Results and Discussion

Overall protein structure and comparisons with lower resolution ba3 structures

The wild type (WT) protein and A120F mutant of ba3 cytochrome c oxidase from T. thermophilus were crystallized in lipidic cubic phase and their structures were refined at 1.8 Å (Table 1). The A120F mutant was originally designed to block one of the mid-membrane entrances to the oxygen channel (see ref.13), but it has no effect on activity or the spectral properties of the enzyme; however, it demonstrates a slightly better crystallization behavior. Both structures are almost identical (total RMSD ∼0.29 Å), while the A120F mutant has a better defined electron density with lower B-factors; therefore, the subsequent analysis will be focused on the A120F mutant unless noted otherwise.

[Figure omitted. See PDF.]

Table 1. Data Collection and Refinement Statistics.

https://doi.org/10.1371/journal.pone.0022348.t001

The enzyme was crystallized under a number of conditions; however, all crystallization conditions contained a high concentration of PEG400 (40–45%) that swells LCP and converts it into a sponge phase [33], which is consistent with a previous hypothesis that large proteins (>50 kDa) require swelling of LCP in order to have enough room for protein molecules to move and feed into growing crystals [34]. The crystals exhibit type I packing (Figure S1), as in all in meso grown crystals to date [35], with one molecule per asymmetric unit in the C2 space group. Each layer in a type I crystal represents a 2D protein crystal with contacts within the layers mediated by protein-lipid or protein-protein interactions and the gaps between protein molecules filled with disordered lipid, forming a lipid bilayer. The protein consists of 3 subunits: a 61.7 kDa core subunit I with 13 transmembrane α-helices (TMHs), containing heme-b, heme-a3 and CuB; an 18.5 kDa mostly water soluble subunit II (the CuA domain) with one TMH, anchoring it to the lipid membrane and to subunit I; and, specific to ba3 oxidase, a 3.8 kDa subunit IIa forming a single TMH, running parallel to the TMH of subunit II.

The overall backbone structure of the ba3 oxidase crystallized in meso (Figure 1) is nearly identical to that crystallized in detergent micelles [9] (total RMSD between the current structure and PDB ID 1XME [9] is ∼0.38 Å). Differences include the conformation of a weakly ordered loop from Ile512 to Arg518 on the inside rim of subunit I, additional ordered waters detected in the interior of the protein, the absence of the glycerol molecule from an interior cavity as seen in 1XME [9], the presence of two O-atoms, possibly a peroxo dianion, that was previously modeled as a single oxygen atom in 1XME [9], and the presence of ordered lipid molecules on the exterior surface of the enzyme.

[Figure omitted. See PDF.]

Figure 1. Crystal structure of the ba3 A120F mutant within implicit lipid bilayer.

Subunit I is shown in cyan, subunit II is shown in orange, and subunit IIa is shown in pink. Monooleins in the asymmetric unit are shown in yellow and symmetry-related lipids are shown in green, with carboxylic carbons depicted as large spheres. Hemes are shown in magenta. Peroxide, Cu2+ and Fe ions are shown by red, brown and cyan spheres, respectively. Red and blue dotted lines indicate periplasmic space and intracellular borders of a hydrophobic slab.

https://doi.org/10.1371/journal.pone.0022348.g001

The in meso structure manifests remarkably low B-factors (Table 1), in contrast to ba3 oxidase crystallized in detergent micelles where average B-factors are >50 Å [8], [9]. Presumably, this is due to the extensive direct and lipid-mediated crystal contacts involving the transmembrane components, but it also reflects the high degree of order within the protein interior (Figure S2). The new structural elements revealed by the high resolution in meso structure include 20 ordered lipid molecules, providing a snapshot of the lipidic environment surrounding ba3 oxidase; 225 ordered water molecules, 53 of which are buried inside the protein, with 29 located in the hydrophilic cavity between subunits I and II where they may be involved in the proton translocation path and the exit route for water molecules from the active site; and a better resolved active site, strongly suggesting placement of a peroxo dianion bound to both the Fe of heme-a3 and to CuB.

Lipid conformations and lipid-protein interactions

The effects of lipids on membrane protein functions have been extensively reported [36]–[38]. Identification of specific lipid-binding sites on the surface of membrane proteins and their conservation through evolution are concepts just recently beginning to emerge [7], [39]–[42]. Since crystallization of ba3-oxidase was performed in a lipidic environment, it is expected that the protein molecules would be surrounded by lipids inside the crystal lattice. Examination of electron density revealed a number of strong elongated density tubes aligned around the protein hydrophobic surface, apparently belonging to lipid molecules. Since no specific native lipid head group density was observed [43], all lipids were modeled as monooleins (the LCP host lipid; PDB chemical component code OLC); however, some of them may represent or mimic ordered hydrocarbon chains of tightly bound native lipids, co-purified with the enzyme. Overall, 20 monoolein molecules were modeled in the asymmetric unit. Within hydrophobic layers in the crystal lattice, each protein interacts with three neighbors. After applying symmetry operations, 36 lipid molecules were revealed in the vicinity of each protein, 30 of which are making direct lipid-protein contacts (4 Å cutoff) (Table 2). Ordered lipids cover 3,500 Å2 out of the total 10,000 Å2 hydrophobic surface of the protein (35%). To date, this structure contains the most complete shell of annular lipids observed for any member of the HCO superfamily. A previous structure of ba3-oxidase (1EHK [8]) has 3 detergent molecules, while the structures of aa3-oxidase from Bos taurus (Bt) (2DYR [3]), Rs (2GSM [7]), and Pd (3HB3 [5]) contain 13 lipids and 6 detergents (39 fatty acid chains covering 11% of the hydrophobic surface), 6 lipids and 5 detergents (10 fatty acid chains covering 13% of the hydrophobic surface), and 24 detergents (24 fatty acid chains covering 33% of the hydrophobic surface), respectively [3], [5], [7], [8]. For comparison, the most complete lipid shells around membrane proteins were resolved in the structures of bacteriorhodopsin crystallized from lipidic cubic phase (27 lipids per protein trimer covering 79% of the trimer hydrophobic surface, PDB ID 1QHJ [44]), a potassium channel crystallized in a lipid/detergent mixture (64 lipids per protein tetramer, PDB ID 2R9R [45]), and two dimensional crystals of aquaporin AQP0 (36 lipids per protein tetramer, PDB ID 2B6O [46]). The new high-resolution structure of ba3-oxidase in the lipidic cubic phase offers the opportunity to visualize details of the lipid bilayer and its interaction with this membrane protein.

[Figure omitted. See PDF.]

Table 2. Summary of Lipid-Protein interactions.

https://doi.org/10.1371/journal.pone.0022348.t002

All lipid molecules in the current ba3-oxidase structure can be grouped in 6 clusters (Figure 2, Table S1). Clusters 1 and 4 are symmetry related and mediate protein interactions in the crystal lattice. Clusters 2 and 3 are associated with the direct hydrophobic protein-protein interaction made by subunit II with itself along the two-fold symmetry axis. Clusters 5 and 6 are not involved in any packing interactions, making them perhaps the most physiologically interesting lipids. Most of the lipid chains exist in extended conformations and are aligned perpendicularly to the membrane surface following grooves between α-helices of ba3. With a few exceptions (e.g. OLC4 and OLC19), the polar head group of the lipid molecules (C24, C22, C21, and C1, with C1 marking the start of the 18-carbon alkyl chain) have the highest B-factor, while the lowest can be found in the middle of the alkyl chain (Figure S3). On average, the lowest B-factor is at C14 of the alkyl chain, about 20% lower than the B-factor of the polar head group. Thus, the properties of lipids composing the annular shell around ba3-oxidase differ from the properties of lipids in a bulk membrane, in which the probability of forming gauche conformations exhibiting higher B-factors increase steeply with increasing carbon position number in the alkyl chain after the first 6-8 carbons [47].

[Figure omitted. See PDF.]

Figure 2. Six clusters of monoolein molecules (green and cyan) surround ba3 oxidase (ribbon structure) in crystals obtained from the lipidic cubic phase.

The copy of ba3 in the asymmetric unit is light blue while symmetry related molecules are shown in gray. The lipid clusters comprise 34 monooleins, 20 of which are in the asymmetric unit. Clusters 4 and 6 mediate protein-protein packing interactions in the lattice. Clusters 2 and 3 flank an extensive direct protein-protein contact on the crystallographic 2-fold axis (space group C2) that involves the entire length of the N-terminal TM domain of Subunit II (CuA domain). Clusters 5 and 6 are associated with the protein in the absence of lattice interactions. Cluster 5 overlaps with the region of ordered lipid and detergent binding sites in other cytochrome oxidases, and contains the unique monoolein, OLC7, which extends above the plane of the implicit bilayer (Figure 3A).

https://doi.org/10.1371/journal.pone.0022348.g002

[Figure omitted. See PDF.]

Figure 3. Superposition of lipids associated with the current ba3 structure and lipids associated wtih three highest resolution aa3 oxidase structures.

Ba3 structure is shown in green (subunit I and IIa) or orange (subunit II) and aa3 is shown in purple. Ba3 lipids are shown in yellow, lipids of the Bos taurus structure (2DYR) in magenta, detergents of the P. denitrificans (3HB3) in blue, and detergents of the R. sphaeroides (2GSM) structure in white. Labels with an * indicate a symmetry mate. Periplasmic and cytoplasmic membrane boundaries are shown in red and blue dotted lines. (A) Close-up of lipids in cluster 5 occupying a conserved region of lipid binding in cytochrome c oxidase and the unique lipid OLC7 that extends out of the membrane, forming specific hydrogen bonds with Arg141 and Glu144. Note that the region occupied by OLC7 in ba3 is filled by the N-terminus of subunit II in aa3 oxidase. (B) Close-up of lipids in cluster 1 occupying a conserved region of lipid binding throughout the cytochrome c oxidases. (C) Close-up of a region near cluster 2 that is conserved for lipid binding in aa3-type oxidases, but not in ba3. Note that the lipid binding region of the aa3 oxidases is occupied by the end of helix 12 of subunit II of ba3 oxidase (shown in orange), while the equivalent helix in aa3-type oxidases (shown in purple) does not extend so far.

https://doi.org/10.1371/journal.pone.0022348.g003

The hydrophobic thickness of the lipidic membrane surrounding ba3-oxidase in the crystal structure can be determined from the average positions of the carboxylic groups of the lipid molecules. This thickness was determined to be 30.1 Å, which agrees well with the Orientations of Proteins in Membranes (OPM) predicted value, 31.4±1.3 Å [48]. The standard deviation of lipid positions along the Z-axis from the average membrane plane is 2 Å, which is comparable to the out-of-plane thermal fluctuations of lipids (2.2 Å [49]). The overall tilt of the protein in respect to the membrane normal is only 8±2°, the same as reported by the OPM server [48].

Individual lipid-protein and lipid-lipid interactions for each of the 20 lipid molecules in the asymmetric unit are described in Table 2. All lipids have direct contacts with at least one ba3 molecule, and for seven of the lipids the contact area exceeds 50% of the total molecular surface. Nine lipid molecules are sandwiched between two proteins in the crystal lattice (OLC 3, 4, 5, 6, 11, 12, 17, 18, 19) with a significant interface with both molecules. In addition, most lipids have a substantial interface with other lipid molecules in the crystal lattice, and presumably with unstructured lipid molecules as well.

Fifteen of twenty lipid molecules in the asymmetric unit have at least one hydrogen bond to ba3 or to other lipid head groups in the crystal structure (Table 2). Nine lipid molecules are hydrogen bonded to the protein, while six have polar interactions only with other lipids. Interestingly, some of the strongest protein-lipid hydrogen bonding networks can be observed for those lipid head groups that are significantly shifted out of the membrane plane. For example, OLC4 is pulled ∼5 Å into the bilayer and has strong polar interactions with side chain and main chain atoms of ba3 subunit I, reflected by the low B-factor of the OLC4 head group (Figure S3). Another interesting example is OLC7, for which the head group protrudes ∼10 Å above the extracellular side of the membrane surface and forms strong hydrogen bonds with the side chain of Arg141 (2.8 and 3.2 Å) and the carbonyl of Glu144 (2.8 Å) in a pocket in the soluble CuA domain (Figure 3A). Eight carbons of the OLC7 hydrocarbon tail are still embedded in the membrane and have tight interactions with other lipid molecules in the structure. The distance between the Cu2 atom of CuA and O23 of OLC7 is ∼20 Å, indicating the absence of a direct functionally important role of this lipid at the CuA site.

Structural superposition of the in meso ba3-oxidase structure with known structures of aa3-oxidases from different species reveals conservation of several lipid binding sites, despite significant differences in protein sequence (Figure S4). A particularly interesting site is centered at cluster 5 (Figure 3A). This region of the protein surface is occupied by the lipid tristearoylglycerol in 2DYR [3], two lauryl dimethylamine-N-oxide detergent molecules in 3HB3 [5], and an ordered tridecane chain in 2GSM [7]. In ba3 oxidase, the lipids in cluster 5 occupy the only concave portion of the protein surface within the membrane and do not participate in crystal packing contacts. This cluster includes two fully ordered molecules, OLC1 and OLC2, as well as the ordered head group of OLC20. OLC1 has distinctly low B-values for its aliphatic tail (Figure S3), and OLCs 1, 2 and 20 surround the translated alkyl chain of OLC7, noted above, which is tethered by strong hydrogen bonding interactions above the plane of the membrane (Figure 3A). Interestingly, while the single TMH of subunit IIa of ba3 terminates near the membrane surface, the spatially equivalent helices in type aa3-oxidases have protein loops extending from this helix that occupy the same region in space as the lipid chain of OLC7. If the OLC7 site is occupied by a native lipid in Tt membranes, where it could interact with Arg141 on subunit II, this positioning of OLC7 could represent an adaptation to the deletion of the homologous protein loop. In particular, a Tt lipid in the OLC7 site could help to tether the subunit I and II domains. At the same time, interactions of OLC1 and OLC2 with subunit IIa may stabilize the association of this TMH, which is inverted in orientation relative to the aa3-oxidases, with subunit I.

Another interesting conserved lipid binding site is in cluster 1 around OLC19, located on the same face of ba3-oxidase as cluster 5, but directed towards the opposite side of the membrane (Figure 3B). This site is occupied by a well-ordered, dodecyl-β-D-maltoside detergent in 3HB3 and an ordered lipid tail in 2GSM. The head group of OLC19 overlays nicely with the polar portion of the detergent molecule in 3HB3. Several other lipids in ba3 occupy similar sites to lipids or detergents observed in one or more aa3-structures. OLC16 and OLC3 occupy similar positions to the two alkyl chains of the phospholipid PGV525 in 2DYR. The alkyl chain of OLC3 occupies a very similar position to the alkyl chain of the detergent LMT572 of 3HB3, while a portion of the detergent head group overlaps with the head groups of both OLC3 and OLC16. The conservation of lipid and detergent binding sites in these oxidase structures suggests that lipid binding occurs in response to elements of the protein fold, and not to specific alkyl tail – hydrophobic side chain interactions (Figure S4).

In addition to lipid/detergent binding sites that are conserved between ba3 and aa3-structures, there are also several sites that are well-conserved among aa3-structures but are not conserved with ba3. For example, two adjacent sites bind detergent molecules in 2GSM and 3HB3, while phospholipid tails from PGV occupy very similar sites in 2DYR. One of these two sites has no lipids present in ba3, potentially because the corresponding TMH α12 (ba3 numbering) with which the lipids interact in aa3-oxidases is significantly displaced in ba3. The second site contains the head group of a symmetry mate of OLC17, occupying a similar position to the polar moieties on the detergents, but in this case the lipid tail extends in a different direction. Another site that is conserved in all three aa3-structures contains a detergent molecule in 3HB3 and 2GSM and a phospholipid tail of PSC in 2DYR. Interestingly, this site does not exist in the structure of ba3: instead, the N-terminus of subunit II extends below the plane of the membrane and occupies the same region in space as the head groups of these lipids (Figure 3C). These examples, and the translation of OLC7 in cluster 5, represent ways in which lipid binding accommodates specific features of membrane protein folds.

Structured water molecules and their possible roles

Evidence continues to accrue that water molecules play important roles in cytochrome c oxidase function, particularly in the coupling of proton-pumping to the reduction of oxygen at the active site of A-type [50], [51] and B-type [12] enzymes. The asymmetric unit of the current model of ba3-oxidase contains 225 water molecules, of which 53 are buried inside the protein (Figure 4A). Remarkably, 29 of these interior waters are found within a hydrophilic and highly irregular cavity in the space between the subunits I and II (Figure 4A). Of these 29, 13 are bound to subunit I, and 10 are bound to subunit II. HOH 69 and 93 bridge between subunits I and II, leaving several internal water molecules with no direct linkage to either subunit. In addition, it is likely that there are other water molecules in this region whose positions are not detected under X-ray diffraction, suggesting the presence of a “sea” of waters between the two subunits (see Koepke et al. [5] for detailed insight on the possible role of this water cluster in oxidase function).

[Figure omitted. See PDF.]

Figure 4. Internal water molecules in the high-resolution structure of ba3 oxidase.

(A) Stick representation of main chain atoms of subunits I (blue) and II (green) of ba3. Water molecules associated with the K-path are shown in yellow; those belonging to the unique cluster (discussed in the text) are shown in cyan; the two water molecules that bridge from CuB into the Xe1 site in the oxygen channel are shown in light purple; and interior waters not thought to have a functional role are shown in gray. Heme-a3 and heme-b are shown in blue sticks, CuA atoms are shown as dark purple spheres; CuB is an orange sphere, and the peroxo dianion is shown as red spheres. The secondary OH group of the gernalygeranyl side chain of heme-a3 is shown in green. (B) Close-up view of the internal water cluster (cyan) and K-path. Side-chains of residues of the K-path are shown in purple with a dot surface, and heme-a3 also has a dot surface. The green sphere in the center corresponds to the secondary OH group of the gernalygeranyl side chain of heme-a3, which also participates in the K-path.

https://doi.org/10.1371/journal.pone.0022348.g004

These 29 water molecules include the semi-conserved cluster above the heme-a3 propionates, a four water cluster (HOHs 26, 64, 103 and 108) at the interface of subunit I and II, and a two water cluster (HOHs 39 and 272) bound exclusively to subunit II. These two small water clusters may serve to pass product water molecules and pumped protons from the internalized water cavity to the external surface of the lipid bilayer, although these may be specific to ba3. The cluster that lies on top of the heme-a3 propionates contains 8 water molecules (cyan, Figures 4, S5, and S6) anchored by interactions with HOH267, which donates two H-bonds to the propionyl carboxylates of heme-a3 and receives a hydrogen bond from the protonated NE atom of His283, one of the ligands to CuB. HOH267 interacts strongly with HOH265, which in turn interacts weakly with other HOH molecules of the cluster. In this manner, the large cluster extends from the heme-a3/CuB binuclear center within subunit I into the water cavity between the subunits, from where, it is reasonable to suggest that it serves to conduct product water molecules and pumped protons out of the protein [5].

Comparison of the new ba3 structure in this region with the two earlier structures of ba3 (1EHK (8) and 1XME (9)) and with that of Bt (2ZXW [51]), Pd (3HB3 [5]), and Rs (2GSM [7]) aa3 enzymes reveal the presence of a similar cluster of 8 to 10 water molecules in which the propionate bridging water is conserved and interacts with one closely situated water that is also conserved (Figure S6). Notably, clusters in Bt and Rs enzymes include a divalent cation (Mg2+ or Mn2+), which has, in the case of the Rs enzyme, been used to trace the atoms of 17O2 reduced at the active site [52]. See Figure S6 for stereo representations of each of these structures. Note that glycerol displaces several of the HOH molecules in the 1XME structure, and in the case of Bt aa3, glycerol is a moderate inhibitor of oxidase activity [53].

The proposed proton-uptake pathway, analogous to the K-path in aa3-type oxidase structures, contains four water molecules resolved in the new structure (yellow, Figures 4 and S7). The pathway may begin at HOH146, residing at the external end of a small “tube” within the protein that extends ∼9 Å to the conserved Glu15B. The internal portion of this proton pathway corresponds to that identified in lower-resolution structures, with possible proton transfer occurring from the protonated carbonyl group of Glu15B to the deprotonated hydroxyl group of Tyr237A via residues Thr315A, HOH102, Tyr248A, Thr312A, a not-yet-identified water molecule spanning the 4.4 Å from Thr312A to Tyr244A, Ser309A, HOH276, and then to the secondary alcohol of HAS, which interacts with the OH of Tyr237A (Figure S7). Another water molecule, HOH80, is in close proximity to HOH276. The not-yet-observed water molecule was previously postulated [12] as a possible structural element to form an intact K-path capable of Grotthus behavior [54]. The absence of any electron density at this position even at 1.8 Å resolution, however, may suggest a different mechanism of proton transfer between Thr312A and Tyr244A. The extended geranylgeranyl tail of heme a3, which runs parallel to the K-path, may serve to stabilize burial of nearby polar side chains and water molecules.

Several of the remaining interior water molecules are involved in hydration of the hemes. HOH71 interacts with the heme-b (HEM) propionate atoms O2A and O2D and with the NE atom of Arg449A. HOH85 also interacts with O2A of heme-b, and HOH82 interacts with O1D of heme-b. Additional solvation of the heme-b propionates involves Arg450. HOH267 interacts with heme-a3 (HAS) propionate atoms O2A and O2D and additionally with the ND atom of the CuB ligand, His283A (Figure S5). Solvation of the D-ring propionate of heme-a3 is completed by interaction of its O2D atom with HOH87 (Figures 4B and S5). There are no other water molecules in the vicinity of heme-a3. The remaining interior waters exist as isolated single or interacting pairs of water molecules that give no indication of involvement in oxidase function, although some of these HOH may be remnants of historical evolution during which the D-channel was lost [12] or possibly pioneer sites of an evolving D-channel.

The oxygen uptake channel

The proposed oxygen channel of ba3 is a continuous, 18–20 Å long, Y-shaped channel lined by hydrophobic residues leading from the membrane-facing surface of the protein into the heme-a3-CuB center (previously described in detail [13]). The new structures confirm earlier data, which suggested that there are no structural waters in the channel [13]. With the exception of one water molecule, HOH165, found at the previously-characterized Xe1 binding site [13] (Figure S8), the 1.8 Å resolution maps show no residual electron density in the Y-shaped channel. The A120F mutation, which was designed with the hope of blocking one of the two entrances to the oxygen channel, is isomorphous with wild type with the Phe120 side chain occluding the entrance to the channel (Figure S8). By itself, this mutation has no effect on the oxidase activity of the mutant form (data not shown), suggesting that the two entrances are redundant and the enzyme can fully function with one of them blocked.

A chain of four oxygen atoms at the active-center end of the channel is comprised of the peroxo dianion (red; PRX O2, see below) bound to CuB and two water molecules (purple) (Figure S8). The chain includes HOH65, which lies 3.0 Å from the CuB-bound peroxo oxygen atom and makes weak contact with the plane of the porphyrin at HAS C3A. In turn, HOH65 hydrogen bonds with HOH165. Both HOH65 and HOH165 have potential hydrogen bonds with the carbonyl of Gly232A (see Figures S8 and S9), which was earlier shown to be part of the O2-channel in the Tt enzyme (see supplementary material of Ref. [13]). HOH65 resides between the innermost Xe site (Xe1) and the O-atom of the peroxo dianion coordinated to CuB. The Tt HOH65 water binding site is in the same position as the HOH6601 water binding site associated with Gly283 in the Rs enzyme [7], and this region of the active site superposes very well in the two proteins. In the Tt structure, HOH65 is above the heme and is flanked by the carbonyl of Gly232, Trp229, and by His283, while in the Rs structure HOH6601 is flanked by the carbonyl of Gly283, Trp280, and His334. Water at position 165 is unique to Tt because it occupies the Xe1 site where it forms a H-bond to the carbonyl of Gly232 (3.4 Å) and to HOH65 (2.64 Å). There is no corresponding HOH in the Rs structure, possibly because the phenol OH of Tyr133 in the Tt structure is replaced with Trp172 in the Rs structure, the side chain of which is flipped up into the O2 channel where it appears to occlude a potential water binding site. It is of considerable interest that the Gly283Val mutation in Rs strongly inhibits access of O2 into the active site [55], although a structure of this mutation has not been reported. Future analyses of conserved water positions throughout the known structures are likely to shed further light on water function in the enzyme.

The previously characterized Xe1 binding site comprises numerous hydrophobic atoms that can contact a xenon atom or a water molecule if present (see supplementary material of Ref. [13]). Alignment of the Xe bearing structure, PDB code 3BVD [13], with our final structural model places HOH165 within the Xe1 site. With respect to the 17 atoms that might interact with Xe1, (see supplementary material of Ref.[13]) the average displacement between Xe1 in 3BVD and HOH165 is only 0.35 Å. It is reasonable to conclude that HOH165 has entered the Xe1 site, most likely from the opposite direction of Xe or O2 molecules. The |Fo| - |Fc| electron density maps provide the first experimental evidence that one of the Xe binding sites can bind water, but there is no evidence, even for partial occupation, of the remaining Xe-binding sites. Hence, the remainder of this large hydrophobic cavity may be empty (see Ref. [56]).

The redox active sites

The ba3-oxidase, like all cytochrome c oxidases, contains four metal-based, redox-active sites. In the new structure, the metrics of the two-copper containing CuA center, situated in subunit II (see Figure 1) and the heme-b are highly similar to those of previous reports (PDB codes 2CUA, 1EHK, and 1XME). The CuA receives electrons from cytochrome c552 while heme-b receives electrons from CuA and donates them to the dioxygen reduction site composed of CuB and the high-spin heme a3 [16]. While the overall structure from the new crystals is not substantially different than the previously-determined structures at 2.3 Å (PDB code 1XME [9]) and at 2.4 Å (PDB code 1EHK [8]), those studies showed that the structure of the heme-a3/CuB site is affected by temperature and time of exposure to the X-ray beams that typify synchrotron sources (see [14], references therein and unpublished work).

The distance between the iron of heme-a3 and the NE-atom of the proximal His384A is 2.2 Å, compared to 3.3 Å in 1EHK and 2.5 Å in 1XME. These are trending towards shorter Fe-N bonding with higher resolution, as seen with the reported distances in Bt aa3 (1.9 Å, PDB code 2DYR [3]), Pd aa3 (2.1 Å, PDB code 3HB3 [5]), and hemoglobin (1.98 Å) [57]. Another difference is the proximity of the CuB to the iron of heme-a3, with a distance of 4.9 Å in the current structure compared to 4.4 Å reported at lower resolution (1XME). This rather large difference is consistent with the idea that the space between Fea3 and CuB is able to fluctuate in response to ongoing chemistry [14]. Other inter-atom distances between the redox centers are comparable if not identical in the three models (Table S2). However, a major difference at the active site of the new structure of ba3, compared to previous structures, is the modeling of a peroxide ion bridging the iron of heme-a3 and CuB. In previous studies (1EHK and 1XME) the electron density in this region was spherical and best, but not well, fit with a single oxygen atom ∼2.3 Å from both iron and CuB. In the current omit map of this region the density is elongated (Figure 5). Refinement was performed in several ways, modeling: (i) a single water, (ii) two waters with partial occupancy, (iii) two O-atoms separated by a fixed distance of 1.47 Å, and (iv) two unrestrained O-atoms. The latter model best fits the density, refining to an O-O distance of 1.52 Å (within error of an expected 1.47 Å distance between the two oxygen atoms in a metal bound, peroxo dianion [58]). The O-metal distances of 2.39 Å to heme a3-Fe and 2.25 Å to CuB are consistent with other structures. In the aa3-type oxidases, a similar metal-bridging ligand has been variously modeled as a single oxygen atom or a peroxide in Rs aa3, 2GSM [7] and in Pd aa3, 1AR1 [59], as an O-O model with distance of 1.7 Å in B t aa3, 2ZXW [51], and as a two oxygen atom arrangement which refined to a distance of 1.64 Å in Pd aa3 [5]. In this recent structure of Pd aa3 (3HB3 [5]), careful refinement with several alternative possibilities led the authors to conclude that the electron density in this region probably represents a peroxo dianion (O-O distance 1.49 Å), and the current structure would support that idea.

[Figure omitted. See PDF.]

Figure 5. Electron density around active site.

2Fo-Fc electron density is shown in green mesh at 1.5σ. The unbiased Fo-Fc difference density is shown in blue at 3.5σ.

https://doi.org/10.1371/journal.pone.0022348.g005

While care should be taken not to impart mechanistic meaning to these observations, there can be little doubt that two O-atoms 1.5 Å apart are in a bonding situation, and the most likely electronic state is that of peroxide, although the protonation states of the O-atoms can only be guessed (see, however, Ref. [60]). Spectroscopic support for a bridging peroxide in the oxidized state of the bovine heart enzyme comes from the work of Sakaguchi et al. [61] who demonstrated the presence of a resonance Raman band at ∼755 cm-1 with 647.1 nm excitation into the 650 nm band of the oxidized protein. Such a frequency is compatible with a peroxide bond length of 1.49 Å; the reported O-O distance of 1.7 Å [51] should thus be reconsidered (see Ref. [60]).

Time-resolved, resonance Raman studies of oxygen reduction have failed to identify a peroxide intermediate (see [11] for references) in the reaction pathway of ba3. Indeed, such an intermediate almost certainly exists, but it must also have an extremely short lifetime, and would not be expected to be trapped, even at quite low temperatures [62]. Most likely, the putative peroxide is formed as a result of X-ray radiation to which the crystals are exposed during data collection. The crystals are formed from the as-isolated enzyme at room temperature, in which spectral properties indicate all the redox cofactors are oxidized. Under this condition no electrons are available to reduce O2, present at ∼1 mM concentrations, to H2O2. The crystals are exposed to X-rays only after freezing at ∼100 K. Therefore, if peroxide is bound in the active site, as the observations suggest, it is most likely formed from atoms present in or very close to the oxidized Fea3/CuB site after being exposed to X-ray radiation (see, however, Footnote 13 of Ref. [2], and Ref. [51] for contrary opinions).

In addition to high-energy X-ray radiation (12 keV) the crystals are also exposed to a flux of H• and OH•, the primary products of water radiolysis. H• transfers an electron to the medium, forming a proton and the hydrated electron, the latter of which has a lifetime in the microsecond range and is highly mobile, even in frozen water. Most likely, it is responsible for reduction of the redox sites in ba3 during collection of X-ray diffraction data [14]. Although we have not recorded optical absorption spectra of our crystals after irradiation, the CuA, heme-b, and heme-a3 sites are undoubtedly reduced. We speculate that the bridging peroxide observed in our data might arise from the recombination of two radiation produced OH• radicals formed either very near to or even in the space between the two metals of the active site.

The orientation of the peroxide between the Fe- and Cu-atoms is different in Tt ba3 than in Pd aa3 (3HB3 [5]), but it is similar to that observed for the putative peroxide in Bt aa3 (3ABL and 2ZXW [51]) (Table 3). In the structure of Pd aa3 (3HB3), the peroxide ion is located just 1.9 Å from the Fe and CuB, and the dihedral angle of heme-a3-O-O-CuB is +175°. The equivalent angle in the current structure of ba3 is −147° (−146.9° in Bt 3ABL), providing a significantly different orientation of the peroxide relative to the plane of heme-a3. A different product from this type of chemistry might be expected if the two putative water molecules were to reside at different positions within the active sites of the different enzymes prior to exposure to X-ray radiation. Whatever the cause, the physiological relevance of this peroxide remains unclear.

[Figure omitted. See PDF.]

Table 3. Summary of observed peroxide geometry.

https://doi.org/10.1371/journal.pone.0022348.t003

Conclusions

We have described a highly-refined, high-resolution structure of cytochrome ba3 oxidase from T. thermophilus from which the following conclusions could be drawn. When crystallized in the lipidic cubic phase, 20 lipids are found surrounding the protein in the asymmetric unit and their interactions with the protein are elucidated. Among these are lipid-protein interactions found in previous structures of aa3-type oxidases that appear to be conserved. One lipid, OLC7, has a previously-unobserved interaction with the water soluble portion of subunit II, lifting the lipid partially out of the lipid bilayer and suggesting that the surface of the lipid bilayer, in close proximity to the protein surface, may be highly irregular. The observed water molecules, most of which are associated with subunit II and the hydrophilic interface between subunits I and II, include a cluster of 8 water molecules. Among this cluster, a previously recognized water molecule bridges the two propionates of heme-a3 and interacts strongly with a second conserved water that traverses the subunit I/subunit II surfaces. In turn, the latter HOH has access to the remaining waters of the cluster that lie outside subunit I. Such a cluster appears to be a conserved feature of A- and B-type cytochromes c oxidases and is likely to be important in both water egress and proton pumping. Finally, the observed active-site density is best interpreted in terms of two O-atoms separated by 1.5 Å, likely a peroxo dianion, that bridges from CuB to Fea3. This feature has been observed in other oxidase structures with varying degrees of clarity. A mechanism for its formation under X-ray radiation is suggested in which two HO• recombine (2 HO• → H2O2) to form peroxide within the active site. This entity is not likely to be of physiological importance. The development of a crystallization system for ba3 oxidase capable of reliable production of crystals diffracting to 1.8 Å or better, in combination with the previously reported expression system for the straightforward generation of ba3 mutants [18], opens the door for future structure-function studies at the single-crystal level.

Materials and Methods

Expression and purification

Recombinant ba3 cytochrome c oxidase was expressed in Thermus thermophilus cells and purified as previously described [18]. The protein was concentrated to 10–15 mg/mL in 1 mM dodecyl-β-D-maltoside detergent solution, and stored at 4°C until used.

Crystallization

Before starting crystallization trials protein solution was spun at 15,000 g for 10 min at 4°C. After spinning, protein was reconstituted in a lipidic cubic phase (LCP) by combining monoolein (Sigma) and protein solution at 3/2 v/v ratio and homogenizing them with a syringe mixer [21], [63]. Crystallization trials were performed in 96-well glass sandwich plates by an in meso crystallization robot [64] using 50 nL protein-laden LCP (lipidic cubic phase) overlaid with 0.8 µL precipitant solution in each well, and sealed with a glass coverslip. Protein reconstitution in LCP and crystallization trials were carried out at room temperature (∼21–23°C). The crystallization plates were stored and imaged in an incubator/imager (RockImager 1000, Formulatrix) at 20°C. Diffraction quality crystals of an average size of 60×50×25 µm were obtained within 14 days in 40–45% (v/v) PEG 400, 1.0 to 1.6 M sodium chloride, 100 mM sodium cacodylate trihydrate pH 5.5–6.5. Crystals were harvested using 50–100 µm nylon loops and immediately flash frozen in liquid nitrogen without adding an extra cryoprotectant.

X-ray data collection and processing

Crystallographic data were collected on the 23ID-B beamline (GM/CA CAT) at the Argonne National Laboratory using a 20 µm collimated minibeam at a wavelength of 1.0330 Å and a MarMosaic 300 detector. To reduce radiation damage crystals were translated to a fresh position, if possible, or replaced after collecting 20 frames at 1 s exposure and 1° oscillation with an unattenuated beam. Datasets were integrated, scaled and merged together using HKL2000 [65]. The wild-type recombinant structure (PDB code 1XME) was used for molecular-replacement calculations with Phaser [66]. The resulting model was refined using Refmac5 and repeated rounds of model adjustment using the ΣA-weighted 2|Fo|-|Fc| and |Fo|-|Fc| electron density maps visualized using MiFit [67]. The coordinates and structure factors of the WT enzyme and of the A120F mutant have been deposited to the Protein Data Bank with accession numbers 3S8F and 3S8G, respectively. Figures were created using PyMOL (http://www.pymol.org) and ICM (Molsoft).

Supporting Information

[Figure omitted. See PDF.]

Figure S1.

Type I crystal packing as observed with in meso grown crystals of ba3. Ba3 chains are shown in gray cartoon, the active site hemes are shown in blue sticks, and lipid molecules are shown as green sticks (O atoms in red). Note the alternating orientation of ba3 molecules in the crystal and the obvious layers.

https://doi.org/10.1371/journal.pone.0022348.s001

(TIF)

Figure S2.

Distribution of thermal displacement B-factors in the ba3 structure. Structure is colored according to B-values (blue: low to red: high). Notice how the interior of the structure is highly ordered and the highest B-values are observed for the exterior lipid molecules.

https://doi.org/10.1371/journal.pone.0022348.s002

(TIF)

Figure S3.

Distribution of B-factor values along hydrocarbon chain of lipid molecules. Head group (glycerol) carbons are numbered C21, C22, C24. The mean value for each carbon position across all 20 lipid molecules is shown by black squares.

https://doi.org/10.1371/journal.pone.0022348.s003

(TIF)

Figure S4.

Structure-based sequence alignment of conserved lipid binding sites in ba3. Alignment is performed between the structures of Tt ba3 (this work), Bt aa3 (2DYR), Rs aa3 (2GSM) and Pd aa3 (3HB3). Although several residues (W426, F429 and H432) are conserved in some of the sites, they form non-specific, non-polar contacts with lipid chains

https://doi.org/10.1371/journal.pone.0022348.s004

(TIF)

Figure S5.

A cluster of eight internal water molecules (cyan) that interact with the heme-a3 propionates and residues of subunits I (blue) and II (green). HOH267 and HOH265 are conserved in other types cytochrome c oxidases and are likely to participate in the transport of product water molecules and pumped protons away from the catalytic center. The hydrophilic cavity is surrounded by polar side chains or main chain atoms of 13 residues, and each of the waters has at least one hydrogen bond with the protein. There is no remaining volume inside the cavity to accommodate additional water molecules. The peroxo dianion is shown in red.

https://doi.org/10.1371/journal.pone.0022348.s005

(TIF)

Figure S6.

Stereo visual structural comparison of the inter-subunit water cluster in four different enzymes. The new ba3 structure (upper left); 1XME, ba3 with glycerol; 1EHK, ba3 original; 2ZXW, Bovine aa3; 2GSM, Rhodobacter sphaeroides aa3; and 3HB3, Paracoccus denitrificans aa3.

https://doi.org/10.1371/journal.pone.0022348.s006

(TIF)

Figure S7.

The proposed proton-uptake pathway (K path) in ba3 oxidase linking the cytosolic surface of the protein to the active center. Side chains of participating residues in subunits I and II (chains A, B) are purple, linking water molecules are yellow, and the secondary alcohol of the heme-a3 side chain is green. Oxygen atoms in the pathway are within hydrogen bonding distance of their nearest neighbors; the gap between Thr312A and Tyr244A (4.4 Å) is expected to be occupied by a not yet resolved water molecule. HOH80 is also within a hydrogen bonding distance to HOH276, Ser309A, and the secondary alcohol. HOH146 at the cytosolic surface of the protein can communicate with Glu15B, a strictly conserved residue, via a ∼9 Å long, loosely packed tube. Water molecules may also access Glu15B via Lys16B at the surface of the subunit II N-terminal transmembrane helix.

https://doi.org/10.1371/journal.pone.0022348.s007

(TIF)

Figure S8.

Comparison of the Y-shaped oxygen channel in the wild type ba3 and in the A120F mutant structures. The channel (green transparent surface) connects the active site of the enzyme with the protein- lipid interface. While in the WT structure both branches of the Y-shaped channel are opened to the surface of the protein (A120 shown in cyan sticks), the F120 side chain in the mutant protein (black sticks) completely blocks one of the openings.

https://doi.org/10.1371/journal.pone.0022348.s008

(TIF)

Figure S9.

A close up of the active center. A chain of four oxygen atoms in the active center includes the peroxo dianion (red), coordinated to the Fe atom of heme-a3 (HAS) and the CuB atom (orange), and two water molecules, HOH65 and HOH165 (purple). HOH165 resides in the Xe1 site, i.e. an expected O2 binding site in the oxygen diffusion channel, nearest to the active center. HOH65 is hydrogen bonded to both the peroxo dianion and HOH165, and both water molecules can form hydrogen bonds with Gly232A in the oxygen diffusion channel. The hydrogen bond between the secondary alcohol of the heme-a3 side chain (green) and Tyr237A represents the terminus of the proton-uptake pathway (Figure S7).

https://doi.org/10.1371/journal.pone.0022348.s009

(TIF)

Table S1.

Assignment of lipids to clusters.

https://doi.org/10.1371/journal.pone.0022348.s010

(PDF)

Table S2.

Comparison of ligand geometry to previous ba3 structures.

https://doi.org/10.1371/journal.pone.0022348.s011

(PDF)

Acknowledgments

The authors acknowledge Robert Fischetti and staff of GM/CA-CAT beamline (23-ID) at Advance Photon Source, Argonne National Laboratory for the development of minibeam and support during data collection, and Stanford Synchrotron Radiation Lightsource staff (beamline 11-1) for support during preliminary crystal screening and evaluation. We thank Yuan Zheng, The Ohio State University, and Martin Caffrey, Trinity College (Dublin, Ireland), for the generous loan of the in meso crystallization robot, V. Mitch Luna for valuable discussions and the anonymous reviewers for helpful criticisms.

Author Contributions

Conceived and designed the experiments: TT WL CDS JAF VC. Performed the experiments: WL YC VC. Analyzed the data: TT WL VK CDS JAF VC. Wrote the paper: TT VK CDS JAF VC.

Citation: Tiefenbrunn T, Liu W, Chen Y, Katritch V, Stout CD, Fee JA, et al. (2011) High Resolution Structure of the ba3 Cytochrome c Oxidase from Thermus thermophilus in a Lipidic Environment. PLoS ONE6(7): e22348. https://doi.org/10.1371/journal.pone.0022348

References

1. Tsukihara T, Aoyama H, Yamashita E, Tomizaki T, Yamaguchi H, et al. (1996) The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A. Science 272: 1136–1144.T. TsukiharaH. AoyamaE. YamashitaT. TomizakiH. Yamaguchi1996The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A.Science27211361144

2. Yoshikawa S, Shinzawa-Itoh K, Nakashima R, Yaono R, Yamashita E, et al. (1998) Redox-coupled crystal structural changes in bovine heart cytochrome c oxidase. Science 280: 1723–1729.S. YoshikawaK. Shinzawa-ItohR. NakashimaR. YaonoE. Yamashita1998Redox-coupled crystal structural changes in bovine heart cytochrome c oxidase.Science28017231729

3. Shinzawa-Itoh K, Aoyama H, Muramoto K, Terada H, Kurauchi T, et al. (2007) Structures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase. EMBO J 26: 1713–1725.K. Shinzawa-ItohH. AoyamaK. MuramotoH. TeradaT. Kurauchi2007Structures and physiological roles of 13 integral lipids of bovine heart cytochrome c oxidase.EMBO J2617131725

4. Iwata S, Ostermeier C, Ludwig B, Michel H (1995) Structure at 2.8 A resolution of cytochrome c oxidase from Paracoccus denitrificans. Nature 376: 660–669.S. IwataC. OstermeierB. LudwigH. Michel1995Structure at 2.8 A resolution of cytochrome c oxidase from Paracoccus denitrificans.Nature376660669

5. Koepke J, Olkhova E, Angerer H, Muller H, Peng G, et al. (2009) High resolution crystal structure of Paracoccus denitrificans cytochrome c oxidase: new insights into the active site and the proton transfer pathways. Biochim Biophys Acta 1787: 635–645.J. KoepkeE. OlkhovaH. AngererH. MullerG. Peng2009High resolution crystal structure of Paracoccus denitrificans cytochrome c oxidase: new insights into the active site and the proton transfer pathways.Biochim Biophys Acta1787635645

6. Svensson-Ek M, Abramson J, Larsson G, Tornroth S, Brzezinski P, et al. (2002) The X-ray crystal structures of wild-type and EQ(I-286) mutant cytochrome c oxidases from Rhodobacter sphaeroides. J Mol Biol 321: 329–339.M. Svensson-EkJ. AbramsonG. LarssonS. TornrothP. Brzezinski2002The X-ray crystal structures of wild-type and EQ(I-286) mutant cytochrome c oxidases from Rhodobacter sphaeroides.J Mol Biol321329339

7. Qin L, Hiser C, Mulichak A, Garavito RM, Ferguson-Miller S (2006) Identification of conserved lipid/detergent-binding sites in a high-resolution structure of the membrane protein cytochrome c oxidase. Proc Natl Acad Sci U S A 103: 16117–16122.L. QinC. HiserA. MulichakRM GaravitoS. Ferguson-Miller2006Identification of conserved lipid/detergent-binding sites in a high-resolution structure of the membrane protein cytochrome c oxidase.Proc Natl Acad Sci U S A1031611716122

8. Soulimane T, Buse G, Bourenkov GP, Bartunik HD, Huber R, et al. (2000) Structure and mechanism of the aberrant ba3-cytochrome c oxidase from Thermus thermophilus. EMBO J 19: 1766–1776.T. SoulimaneG. BuseGP BourenkovHD BartunikR. Huber2000Structure and mechanism of the aberrant ba3-cytochrome c oxidase from Thermus thermophilus.EMBO J1917661776

9. Hunsicker-Wang LM, Pacoma RL, Chen Y, Fee JA, Stout CD (2005) A novel cryoprotection scheme for enhancing the diffraction of crystals of recombinant cytochrome ba3 oxidase from Thermus thermophilus. Acta Crystallogr D Biol Crystallogr 61: 340–343.LM Hunsicker-WangRL PacomaY. ChenJA FeeCD Stout2005A novel cryoprotection scheme for enhancing the diffraction of crystals of recombinant cytochrome ba3 oxidase from Thermus thermophilus.Acta Crystallogr D Biol Crystallogr61340343

10. Buschmann S, Warkentin E, Xie H, Langer JD, Ermler U, et al. (2010) The structure of cbb3 cytochrome oxidase provides insights into proton pumping. Science 329: 327–330.S. BuschmannE. WarkentinH. XieJD LangerU. Ermler2010The structure of cbb3 cytochrome oxidase provides insights into proton pumping.Science329327330

11. Fee JA, Case DA, Noodleman L (2008) Toward a chemical mechanism of proton pumping by the B-type cytochrome c oxidases: application of density functional theory to cytochrome ba3 of Thermus thermophilus. J Am Chem Soc 130: 15002–15021.JA FeeDA CaseL. Noodleman2008Toward a chemical mechanism of proton pumping by the B-type cytochrome c oxidases: application of density functional theory to cytochrome ba3 of Thermus thermophilus.J Am Chem Soc1301500215021

12. Chang HY, Hemp J, Chen Y, Fee JA, Gennis RB (2009) The cytochrome ba3 oxygen reductase from Thermus thermophilus uses a single input channel for proton delivery to the active site and for proton pumping. Proc Natl Acad Sci U S A 106: 16169–16173.HY ChangJ. HempY. ChenJA FeeRB Gennis2009The cytochrome ba3 oxygen reductase from Thermus thermophilus uses a single input channel for proton delivery to the active site and for proton pumping.Proc Natl Acad Sci U S A1061616916173

13. Luna VM, Chen Y, Fee JA, Stout CD (2008) Crystallographic studies of Xe and Kr binding within the large internal cavity of cytochrome ba3 from Thermus thermophilus: structural analysis and role of oxygen transport channels in the heme-Cu oxidases. Biochemistry 47: 4657–4665.VM LunaY. ChenJA FeeCD Stout2008Crystallographic studies of Xe and Kr binding within the large internal cavity of cytochrome ba3 from Thermus thermophilus: structural analysis and role of oxygen transport channels in the heme-Cu oxidases.Biochemistry4746574665

14. Liu B, Chen Y, Doukov T, Soltis SM, Stout CD, et al. (2009) Combined microspectrophotometric and crystallographic examination of chemically reduced and X-ray radiation-reduced forms of cytochrome ba3 oxidase from Thermus thermophilus: structure of the reduced form of the enzyme. Biochemistry 48: 820–826.B. LiuY. ChenT. DoukovSM SoltisCD Stout2009Combined microspectrophotometric and crystallographic examination of chemically reduced and X-ray radiation-reduced forms of cytochrome ba3 oxidase from Thermus thermophilus: structure of the reduced form of the enzyme.Biochemistry48820826

15. Zimmermann BH, Nitsche CI, Fee JA, Rusnak F, Munck E (1988) Properties of a copper-containing cytochrome ba3: a second terminal oxidase from the extreme thermophile Thermus thermophilus. Proc Natl Acad Sci U S A 85: 5779–5783.BH ZimmermannCI NitscheJA FeeF. RusnakE. Munck1988Properties of a copper-containing cytochrome ba3: a second terminal oxidase from the extreme thermophile Thermus thermophilus.Proc Natl Acad Sci U S A 8557795783

16. Farver O, Wherland S, Antholine WE, Gemmen GJ, Chen Y, et al. (2010) Pulse Radiolysis Studies of Temperature Dependent Electron Transfers among Redox Centers in ba(3)-Cytochrome c Oxidase from Thermus thermophilus: Comparison of A- and B-Type Enzymes. Biochemistry published on web. O. FarverS. WherlandWE AntholineGJ GemmenY. Chen2010Pulse Radiolysis Studies of Temperature Dependent Electron Transfers among Redox Centers in ba(3)-Cytochrome c Oxidase from Thermus thermophilus: Comparison of A- and B-Type Enzymes.Biochemistry published on webDOI:10.1021/bi100548n. DOI:10.1021/bi100548n.

17. Keightley JA, Zimmermann BH, Mather MW, Springer P, Pastuszyn A, et al. (1995) Molecular genetic and protein chemical characterization of the cytochrome ba3 from Thermus thermophilus HB8. J Biol Chem 270: 20345–20358.JA KeightleyBH ZimmermannMW MatherP. SpringerA. Pastuszyn1995Molecular genetic and protein chemical characterization of the cytochrome ba3 from Thermus thermophilus HB8.J Biol Chem2702034520358

18. Chen Y, Hunsicker-Wang L, Pacoma RL, Luna E, Fee JA (2005) A homologous expression system for obtaining engineered cytochrome ba3 from Thermus thermophilus HB8. Protein Expr Purif 40: 299–318.Y. ChenL. Hunsicker-WangRL PacomaE. LunaJA Fee2005A homologous expression system for obtaining engineered cytochrome ba3 from Thermus thermophilus HB8.Protein Expr Purif40299318

19. Smirnova I, Reimann J, von Ballmoos C, Chang HY, Gennis RB, et al. (2010) Functional role of Thr-312 and Thr-315 in the proton-transporting pathway in ba3 cytochrome c oxidase from Thermus thermophilus. Biochemistry 49: 7033–7039.I. SmirnovaJ. ReimannC. von BallmoosHY ChangRB Gennis2010Functional role of Thr-312 and Thr-315 in the proton-transporting pathway in ba3 cytochrome c oxidase from Thermus thermophilus.Biochemistry4970337039

20. Landau EM, Rosenbusch JP (1996) Lipidic cubic phases: a novel concept for the crystallization of membrane proteins. Proc Natl Acad Sci U S A 93: 14532–14535.EM LandauJP Rosenbusch1996Lipidic cubic phases: a novel concept for the crystallization of membrane proteins.Proc Natl Acad Sci U S A931453214535

21. Caffrey M, Cherezov V (2009) Crystallizing membrane proteins using lipidic mesophases. Nat Protoc 4: 706–731.M. CaffreyV. Cherezov2009Crystallizing membrane proteins using lipidic mesophases.Nat Protoc4706731

22. Pebay-Peyroula E, Rummel G, Rosenbusch JP, Landau EM (1997) X-ray structure of bacteriorhodopsin at 2.5 angstroms from microcrystals grown in lipidic cubic phases. Science 277: 1676–1681.E. Pebay-PeyroulaG. RummelJP RosenbuschEM Landau1997X-ray structure of bacteriorhodopsin at 2.5 angstroms from microcrystals grown in lipidic cubic phases.Science27716761681

23. Luecke H, Schobert B, Richter HT, Cartailler JP, Lanyi JK (1999) Structure of bacteriorhodopsin at 1.55 A resolution. J Mol Biol 291: 899–911.H. LueckeB. SchobertHT RichterJP CartaillerJK Lanyi1999Structure of bacteriorhodopsin at 1.55 A resolution.J Mol Biol291899911

24. Kolbe M, Besir H, Essen LO, Oesterhelt D (2000) Structure of the light-driven chloride pump halorhodopsin at 1.8 A resolution. Science 288: 1390–1396.M. KolbeH. BesirLO EssenD. Oesterhelt2000Structure of the light-driven chloride pump halorhodopsin at 1.8 A resolution.Science28813901396

25. Royant A, Nollert P, Edman K, Neutze R, Landau EM, et al. (2001) X-ray structure of sensory rhodopsin II at 2.1-A resolution. Proc Natl Acad Sci U S A 98: 10131–10136.A. RoyantP. NollertK. EdmanR. NeutzeEM Landau2001X-ray structure of sensory rhodopsin II at 2.1-A resolution.Proc Natl Acad Sci U S A981013110136

26. Gordeliy VI, Labahn J, Moukhametzianov R, Efremov R, Granzin J, et al. (2002) Molecular basis of transmembrane signalling by sensory rhodopsin II-transducer complex. Nature 419: 484–487.VI GordeliyJ. LabahnR. MoukhametzianovR. EfremovJ. Granzin2002Molecular basis of transmembrane signalling by sensory rhodopsin II-transducer complex.Nature419484487

27. Vogeley L, Sineshchekov OA, Trivedi VD, Sasaki J, Spudich JL, et al. (2004) Anabaena sensory rhodopsin: a photochromic color sensor at 2.0 A. Science 306: 1390–1393.L. VogeleyOA SineshchekovVD TrivediJ. SasakiJL Spudich2004Anabaena sensory rhodopsin: a photochromic color sensor at 2.0 A.Science30613901393

28. Cherezov V, Rosenbaum DM, Hanson MA, Rasmussen SG, Thian FS, et al. (2007) High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor. Science 318: 1258–1265.V. CherezovDM RosenbaumMA HansonSG RasmussenFS Thian2007High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor.Science31812581265

29. Jaakola VP, Griffith MT, Hanson MA, Cherezov V, Chien EY, et al. (2008) The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist. Science 322: 1211–1217.VP JaakolaMT GriffithMA HansonV. CherezovEY Chien2008The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist.Science32212111217

30. Wu B, Chien EY, Mol CD, Fenalti G, Liu W, et al. (2010) Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists. Science 330: 1066–1071.B. WuEY ChienCD MolG. FenaltiW. Liu2010Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists.Science33010661071

31. Chien EY, Liu W, Zhao Q, Katritch V, Han GW, et al. (2010) Structure of the Human Dopamine D3 Receptor in Complex with a D2/D3 Selective Antagonist. Science 330: 1091–1095.EY ChienW. LiuQ. ZhaoV. KatritchGW Han2010Structure of the Human Dopamine D3 Receptor in Complex with a D2/D3 Selective Antagonist.Science33010911095

32. Hanson MA, Cherezov V, Griffith MT, Roth CB, Jaakola VP, et al. (2008) A specific cholesterol binding site is established by the 2.8 A structure of the human beta2-adrenergic receptor. Structure 16: 897–905.MA HansonV. CherezovMT GriffithCB RothVP Jaakola2008A specific cholesterol binding site is established by the 2.8 A structure of the human beta2-adrenergic receptor.Structure16897905

33. Cherezov V, Liu W, Derrick JP, Luan B, Aksimentiev A, et al. (2008) In meso crystal structure and docking simulations suggest an alternative proteoglycan binding site in the OpcA outer membrane adhesin. Proteins 71: 24–34.V. CherezovW. LiuJP DerrickB. LuanA. Aksimentiev2008In meso crystal structure and docking simulations suggest an alternative proteoglycan binding site in the OpcA outer membrane adhesin.Proteins712434

34. Cherezov V, Clogston J, Papiz MZ, Caffrey M (2006) Room to move: crystallizing membrane proteins in swollen lipidic mesophases. J Mol Biol 357: 1605–1618.V. CherezovJ. ClogstonMZ PapizM. Caffrey2006Room to move: crystallizing membrane proteins in swollen lipidic mesophases.J Mol Biol35716051618

35. Caffrey M (2009) Crystallizing membrane proteins for structure determination: use of lipidic mesophases. Annu Rev Biophys 38: 29–51.M. Caffrey2009Crystallizing membrane proteins for structure determination: use of lipidic mesophases.Annu Rev Biophys382951

36. Loh HH, Law PY (1980) The role of membrane lipids in receptor mechanisms. Annu Rev Pharmacol Toxicol 20: 201–234.HH LohPY Law1980The role of membrane lipids in receptor mechanisms.Annu Rev Pharmacol Toxicol20201234

37. Lee AG (2004) How lipids affect the activities of integral membrane proteins. Biochim Biophys Acta 1666: 62–87.AG Lee2004How lipids affect the activities of integral membrane proteins.Biochim Biophys Acta16666287

38. Robinson NC (1993) Functional binding of cardiolipin to cytochrome c oxidase. J Bioenerg Biomembr 25: 153–163.NC Robinson1993Functional binding of cardiolipin to cytochrome c oxidase.J Bioenerg Biomembr25153163

39. Qin L, Sharpe MA, Garavito RM, Ferguson-Miller S (2007) Conserved lipid-binding sites in membrane proteins: a focus on cytochrome c oxidase. Curr Opin Struct Biol 17: 444–450.L. QinMA SharpeRM GaravitoS. Ferguson-Miller2007Conserved lipid-binding sites in membrane proteins: a focus on cytochrome c oxidase.Curr Opin Struct Biol17444450

40. Sedlak E, Panda M, Dale MP, Weintraub ST, Robinson NC (2006) Photolabeling of cardiolipin binding subunits within bovine heart cytochrome c oxidase. Biochemistry 45: 746–754.E. SedlakM. PandaMP DaleST WeintraubNC Robinson2006Photolabeling of cardiolipin binding subunits within bovine heart cytochrome c oxidase.Biochemistry45746754

41. Hunte C, Richers S (2008) Lipids and membrane protein structures. Curr Opin Struct Biol 18: 406–411.C. HunteS. Richers2008Lipids and membrane protein structures.Curr Opin Struct Biol18406411

42. Reichow SL, Gonen T (2009) Lipid-protein interactions probed by electron crystallography. Curr Opin Struct Biol 19: 560–565.SL ReichowT. Gonen2009Lipid-protein interactions probed by electron crystallography.Curr Opin Struct Biol19560565

43. Yang YL, Yang FL, Jao SC, Chen MY, Tsay SS, et al. (2006) Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus. J Lipid Res 47: 1823–1832.YL YangFL YangSC JaoMY ChenSS Tsay2006Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus.J Lipid Res4718231832

44. Belrhali H, Nollert P, Royant A, Menzel C, Rosenbusch JP, et al. (1999) Protein, lipid and water organization in bacteriorhodopsin crystals: a molecular view of the purple membrane at 1.9 A resolution. Structure 7: 909–917.H. BelrhaliP. NollertA. RoyantC. MenzelJP Rosenbusch1999Protein, lipid and water organization in bacteriorhodopsin crystals: a molecular view of the purple membrane at 1.9 A resolution.Structure7909917

45. Long SB, Tao X, Campbell EB, MacKinnon R (2007) Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment. Nature 450: 376–382.SB LongX. TaoEB CampbellR. MacKinnon2007Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment.Nature450376382

46. Gonen T, Cheng Y, Sliz P, Hiroaki Y, Fujiyoshi Y, et al. (2005) Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature 438: 633–638.T. GonenY. ChengP. SlizY. HiroakiY. Fujiyoshi2005Lipid-protein interactions in double-layered two-dimensional AQP0 crystals.Nature438633638

47. Seelig A, Seelig J (1977) Effect of single cis double bound on the structure of a phospholipid bilayer. Biochemistry 16: 45–50.A. SeeligJ. Seelig1977Effect of single cis double bound on the structure of a phospholipid bilayer.Biochemistry164550

48. Lomize MA, Lomize AL, Pogozheva ID, Mosberg HI (2006) OPM: orientations of proteins in membranes database. Bioinformatics 22: 623–625.MA LomizeAL LomizeID PogozhevaHI Mosberg2006OPM: orientations of proteins in membranes database.Bioinformatics22623625

49. Hoyrup P, Callisen TH, Jensen MO, Halperin A, Mouritsen OG (2004) Lipid protrusions, membrane softness, and enzymatic activity. Phys Chem Chem Phys 6: 1608–1615.P. HoyrupTH CallisenMO JensenA. HalperinOG Mouritsen2004Lipid protrusions, membrane softness, and enzymatic activity.Phys Chem Chem Phys616081615

50. Lee HJ, Svahn E, Swanson JM, Lepp H, Voth GA et al (2010) Intricate Role of Water in Proton Transport through Cytochrome c Oxidase. J Am Chem Soc 132: 16225–16239.HJ LeeE. SvahnJM SwansonH. Leppal Voth GA et2010Intricate Role of Water in Proton Transport through Cytochrome c Oxidase.J Am Chem Soc1321622516239

51. Aoyama H, Muramoto K, Shinzawa-Itoh K, Hirata K, Yamashita E, et al. (2009) A peroxide bridge between Fe and Cu ions in the O2 reduction site of fully oxidized cytochrome c oxidase could suppress the proton pump. Proc Natl Acad Sci U S A 106: 2165–2169.H. AoyamaK. MuramotoK. Shinzawa-ItohK. HirataE. Yamashita2009A peroxide bridge between Fe and Cu ions in the O2 reduction site of fully oxidized cytochrome c oxidase could suppress the proton pump.Proc Natl Acad Sci U S A10621652169

52. Schmidt B, McCracken J, Ferguson-Miller S (2003) A discrete water exit pathway in the membrane protein cytochrome c oxidase, Proc Natl Acad Sci U S A 100: 15539–15542.B. SchmidtJ. McCrackenS. Ferguson-Miller2003A discrete water exit pathway in the membrane protein cytochrome c oxidase,Proc Natl Acad Sci U S A1001553915542

53. Einarsdottir O, Choc MG, Weldon S, Caughey WS (1988) The site and mechanism of dioxygen reduction in bovine heart cytochrome c oxidase. J Biol Chem 263: 13641–13654.O. EinarsdottirMG ChocS. WeldonWS Caughey1988The site and mechanism of dioxygen reduction in bovine heart cytochrome c oxidase.J Biol Chem2631364113654

54. Agmon N (1995) The Grotthuss mechanism. Chem Phys Lett 244: 456– 462.N. Agmon1995The Grotthuss mechanism.Chem Phys Lett244456 462

55. Salomonsson L, Lee A, Gennis RB, Brzezinski P (2004) A single amino-acid lid renders a gas-tight compartment within a membrane-bound transporter. Proc Natl Acad Sci U S A 101: 11617–11621.L. SalomonssonA. LeeRB GennisP. Brzezinski2004A single amino-acid lid renders a gas-tight compartment within a membrane-bound transporter.Proc Natl Acad Sci U S A1011161711621

56. Yin H, Feng G, Clore GM, Hummer G, Rasaiah JC (2010) Water in the polar and nonpolar cavities of the protein Interleukin-1-beta. J Phys Chem B 114: 16290–16297.H. YinG. FengGM CloreG. HummerJC Rasaiah2010Water in the polar and nonpolar cavities of the protein Interleukin-1-beta.J Phys Chem B1141629016297

57. Eisenberger P, Shulman RG, Brown GS, Ogawa S (1976) Structure-function relations in hemoglobin as determined by x-ray absorption spectroscopy. Proc Natl Acad Sci U S A 73: 491–495.P. EisenbergerRG ShulmanGS BrownS. Ogawa1976Structure-function relations in hemoglobin as determined by x-ray absorption spectroscopy.Proc Natl Acad Sci U S A73491495

58. Chishiro T, Shimazaki Y, Tani F, Tachi Y, Naruta Y, et al. (2003) Isolation and crystal structure of a peroxo-brodged heme-copper complex. Ang Chem Int Ed 42: 2788– 2791.T. ChishiroY. ShimazakiF. TaniY. TachiY. Naruta2003Isolation and crystal structure of a peroxo-brodged heme-copper complex.Ang Chem Int Ed422788 2791

59. Ostermeier C, Harrenga A, Ermler U, Michel H (1997) Structure at 2.7 A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody FV fragment. Proc Natl Acad Sci U S A 94: 10547–10553.C. OstermeierA. HarrengaU. ErmlerH. Michel1997Structure at 2.7 A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody FV fragment.Proc Natl Acad Sci U S A941054710553

60. Kaila VRI, Oksanen E, Goldman A, Bloch D, Verkhovsky MI, et al. (2011) A combined quantum chemical and crystallographic study on the oxidized binuclear center of cytochrome c oxidase. Biochim Biophys Acta. 1807. : 769–778.VRI KailaE. OksanenA. GoldmanD. BlochMI Verkhovsky2011A combined quantum chemical and crystallographic study on the oxidized binuclear center of cytochrome c oxidase.Biochim Biophys Acta1807769778

61. Sakaguchi M, Shinzawa-Itoh K, Yoshikawa S, Ogura T (2010) A resonance Raman band assignable to the O-O stretching mode in the resting oxidized state of bovine heart cytochrome c oxidise. J Bioenerg Biomembr 42: 241–243.M. SakaguchiK. Shinzawa-ItohS. YoshikawaT. Ogura2010A resonance Raman band assignable to the O-O stretching mode in the resting oxidized state of bovine heart cytochrome c oxidise.J Bioenerg Biomembr42241243

62. Chance B, Saronio C, Waring A, Leigh JS Jr (1978) Cytochrome c-cytochrome oxidase interactions at subzero temperatures. Biochim. Biophys. Acta 503: 37–55.B. ChanceC. SaronioA. WaringJS Leigh Jr1978Cytochrome c-cytochrome oxidase interactions at subzero temperatures. Biochim. Biophys.Acta 5033755

63. Cheng A, Hummel B, Qiu H, Caffrey M (1998) A simple mechanical mixer for small viscous lipid-containing samples. Chem Phys Lipids 95: 11–21.A. ChengB. HummelH. QiuM. Caffrey1998A simple mechanical mixer for small viscous lipid-containing samples.Chem Phys Lipids951121

64. Cherezov V, Peddi A, Muthusubramaniam L, Zheng YF, Caffrey M (2004) A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Acta Crystallogr D Biol Crystallogr 60: 1795–1807.V. CherezovA. PeddiL. MuthusubramaniamYF ZhengM. Caffrey2004A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases.Acta Crystallogr D Biol Crystallogr6017951807

65. Minor W, Cymborowski M, Otwinowski Z, Chruszcz M (2006) HKL-3000: the integration of data reduction and structure solution - from diffraction images to an initial model in minutes. Acta Crystallogr D Biol Crystallogr 62: 859–866.W. MinorM. CymborowskiZ. OtwinowskiM. Chruszcz2006HKL-3000: the integration of data reduction and structure solution - from diffraction images to an initial model in minutes.Acta Crystallogr D Biol Crystallogr62859866

66. McCoy AJ, Grosse-Kunstleve RW, Adams PD, Winn MD, Storoni LC, et al. (2007) Phaser crystallographic software. J Appl Crystallogr 40: 658–674.AJ McCoyRW Grosse-KunstlevePD AdamsMD WinnLC Storoni2007Phaser crystallographic software.J Appl Crystallogr40658674

67. McRee DE (2004) Differential evolution for protein crystallographic optimizations. Acta Crystallogr D Biol Crystallogr 60: 2276–2279.DE McRee2004Differential evolution for protein crystallographic optimizations.Acta Crystallogr D Biol Crystallogr6022762279

68. Unno M, Chen H, Kusama S, Shaik S, Ikeda-Saito M (2007) Structural characterization of the fleeting ferric peroxo species in myoglobin, Experiment and theory. J Am Chem Soc 129: 13394–13395.M. UnnoH. ChenS. KusamaS. ShaikM. Ikeda-Saito2007Structural characterization of the fleeting ferric peroxo species in myoglobin, Experiment and theory.J Am Chem Soc1291339413395

69. Kuhnel K, Derat E, Terner J, Shaik S, Schlicting I (2007) Structure and quantum chemical characterization of chloroperoxidase compound 0, a common reaction intermediate of diverse heme enzymes. Proc Natl Acad Sci U S A 104: 99–104.K. KuhnelE. DeratJ. TernerS. ShaikI. Schlicting2007Structure and quantum chemical characterization of chloroperoxidase compound 0, a common reaction intermediate of diverse heme enzymes.Proc Natl Acad Sci U S A10499104