Abstract

Doc number: 23

Abstract

Background: Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level.

Results: In this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethylation450 Beadchip, along with DNA from matched frozen buffy coats. We obtained high quality and reproducible genome-wide DNA methylation profiles using both sample types. We also report high correlations (r > 0.9907) between DNA obtained from matched dried blood spots and frozen buffy coats, sufficient to distinguish between unrelated individuals.

Conclusions: We, thus, demonstrate that DNA from archived dried blood spots is suitable for genome-wide DNA methylation profiling.

Details

Title
The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array
Author
Joo, JiHoon E; Wong, Ee Ming; Baglietto, Laura; Jung, Chol-Hee; Tsimiklis, Helen; Park, Daniel J; Wong, Nicholas C; English, Dallas R; Hopper, John L; Severi, Gianluca; Giles, Graham G; Southey, Melissa C
Pages
23
Publication year
2013
Publication date
2013
Publisher
BioMed Central
e-ISSN
14726750
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1472-6750-13-23
ProQuest document ID
1319786347
Copyright
© 2013 Joo et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.