First record of Brevimulticaecum larvae (Nematoda, Heterocheilidae) in amphibians from northern Argentina

Abstract

Brevimulticaecum Mozgovoy, in Skrjabin, Shikhobalova, and Mozgovoy, 1951 was redefined and presented as a valid genus by Sprent. The new denomination proposed by Sprent is based on the following morphological characteristics: flat lips with a late margins and notches, without dentigerous ridges. The excretory pore in Brevimulticaecum is located in front of or at the nerve ring, and the ventriculus presents short appendices. One Lepidobatrachus laevis Budgett, 1899 was collected in Ingeniero Juarez, Formosa, Argentina and 34 Physalaemus albonotatus and 19 Pseudis paradoxa were collected near the city of Corrientes, Corrientes, Argentina between March 2010 and February 2011. At necropsy, all the organs were examined for parasites by dissection. Capsules were counted and isolated from host tissues. Larvae were removed from capsules using preparation needles and studied first in vivo, enumerated, and then killed in hot distilled water, preserved in 70% ethyl alcohol and examined as temporary mounts.