Avaliação da influência do formol e do hipoclorito de sódio na pesquisa de oocistos de Cryptosporidium nas fezes, através do método de heine

Abstract

Cryptosporidium oocysts were searched by Heine 's method in stools of nine cal/s with cryptoporidiosis after stool treatment with two disinfectants, 10% paraformaldehyde solution and 14,5% sodium hypochlorite solution. After 30 minutes exposition to sodium hypochlorite solution oocysts became non refractile and acquired a reddish tinge, making their Identification difficult. No morphological alterations occured in oocysts after paraformaldehyde treatment. We recommend paraformaldehyde at 10% concentration as means of human immunodeficiency virus (HIV) inactivation for routine use in stool examinations and therefore making safer those type of procedures for laboratory personnel, when using Heine 's method.

ARTIGOS

Avaliação da influência do formol e do hipoclorito de sódio na pesquisa de oocistos de Cryptosporidium nas fezes, através do método de heine

José Rafael Módolo; Vicente Amato Neto; Lúcia Maria Almeida Braz; Carlos Alberto Magalhães Lopes; Arnold Frederico Gottschalk

Endereço para correspondência

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RESUMO

Foram examinadas, para pesquisa de Cryptosporidium pelo método de Heine,fezes de nove bezerros com criptosporidíase, após utilização prévia de dois diferentes desinfetantes. Quanto ao formol a 10%, notou-se que não houve interferência na identificação dos oocistos, em período compreendido entre cinco minutos e 72 horas; ao ser usado o hipoclorito de sódio a 14,5%, verificou-se que depois de 30minutos os ooçistos apresentaram-se avermelhados e sem refração, dificultando o reconhecimento. Assim, recomenda-se a adição de formol a 10% à matéria fecal, conforme a etapa referida, para coibir o risco de infecção de laboratoristas pelo vírus da imunodeficiência humana (HIV), quando usada para diagnóstico atécnica mencionada.

Palavras-chave: Criptosporidíase. Método de Heine. Desinfetantes. Inativação do vírus da imunodeficiência humana (HIV).

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ABSTRACT

Cryptosporidium oocysts were searched by Heine 's method in stools of nine cal/s with cryptoporidiosis after stool treatment with two disinfectants, 10% paraformaldehyde solution and 14,5% sodium hypochlorite solution. After 30 minutes exposition to sodium hypochlorite solution oocysts became non refractile and acquired a reddish tinge, making their Identification difficult. No morphological alterations occured in oocysts after paraformaldehyde treatment. We recommend paraformaldehyde at 10% concentration as means of human immunodeficiency virus (HIV) inactivation for routine use in stool examinations and therefore making safer those type of procedures for laboratory personnel, when using Heine 's method.

Keywords: Cryptosporidiosis. Heine's method. Disinfectants. Human immunodeficiency virus (HIV) inactivation.

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Endereço para correspondência:

Dr. José Rafael Módolo.

Laboratório de Planejamento de Saúde Animal e de Saúde Pública.

Distrito de Rubião Júnior.

Caixa Postal 524

18600- 400

Botucatu, SP, Brasil.

Recebido para publicação em 05/10/93.

Faculdade de Medicina Veterinária e Zootecnia de Botucatu e Instituto de Biociências de Botucatu, da Universidade Estadual Paulista, Botucatu, SP e Laboratório de Investigação Médica- Parasitologia, do Hospital das Clínicas, da Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP.