Abstract

Doc number: 24

Abstract

Background: Transforming growth factor beta (TGF-β1) is a pleiotropic cytokine, which is deregulated in atherosclerosis; however the role of age in this process is unknown. We aimed to assess whether TGF-β1 signaling is affected by age.

Methods: Vascular smooth muscle cells (VSMC) were obtained from patients undergoing abdominal surgery. Levels of TGF-β1 were measured by ELISA in sera from 169 patients undergoing coronary artery bypass grafting (CABG). The p27 expression was determined by Western blot from internal mammary arteries (IMA) obtained from CABG patients (n = 13). In VSMC from these patients undergoing abdominal surgery, secretion of TGF-β1 was determined by ELISA of cell-conditioned media.

Results: In VSMC from aged patients we observed a lower TGF-β1 secretion, measured as TGF-β1 concentration in cell conditioned medium (p < 0.001). This effect was correlated to an age-dependent decrease of p27 expression in IMA from aged CABG patients. In a similar manner, there was an age-dependent decrease of serum TGF-β1 levels in CABG patients (p = 0.0195).

Conclusions: VSMC from aged patients showed a higher degree of cellular senescence and it was associated to a lower TGF-β1 secretion and signaling.

Details

Title
Age-dependent defective TGF-beta1 signaling in patients undergoing coronary artery bypass grafting
Author
Redondo, Santiago; Navarro-Dorado, Jorge; Ramajo, Marta; Medina, Úrsula; Molina-Sanchez, Pedro; Garces, Zaady; García-Alonso, Mauricio; Reguillo, Fernando; Rodriguez, Enrique; Andres, Vicente; Tejerina, Teresa
Pages
24
Publication year
2014
Publication date
2014
Publisher
BioMed Central
e-ISSN
1749-8090
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1498144446
Copyright
© 2014 Redondo et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.