AGE AND GROWTH OF CAPOETA ERHANI (ACTINOPTERYGII: CYPRINIFORMES: CYPRINIDAE) FROM THE MENZELET RESERVOIR, TURKEY

Abstract

Capoeta erhani Turan, Kottelat et Ekmekci, 2008 has an economic importance in commercial and recreational fisheries in Turkey. Knowledge of the biology of this species is very scarce. Despite the importance of this species, no research on the population dynamics such as age and growth rates has been conducted. The aim of this study was to determine the age, growth rate, and the relations between the fish size and the otolith size for C. erhani from the Menzelet Reservoir, Turkey. This study was carried out seasonally in the Menzelet Reservoir, between January and December 2012. Samples were collected using gill nets with mesh sizes ranging from 18 to 30 mm. This study provides the first information on age, growth, and otolith morphometric parameters of Capoeta erhani. Determining the parameters of C. erhani age and growth rates will lead to the estimates of relevant parameters of population dynamics and to better understanding of the long-term changes of the stock sizes.

Headnote

Ayyildiz H., Emre Y., Ozen O., Yagci A. 2014. Age and growth of Capoeta erhani (Actinopterygii: Cypriniformes: Cyprinidae) from the Menzelet Reservoir, Turkey. Acta Ichthyol. Piscat. 44 (2): 105-110.

Background. Capoeta erhani Turan, Kottelat et Ekmekçi, 2008 has an economic importance in commercial and recreational fisheries in Turkey. Knowledge of the biology of this species is very scarce. Despite the importance of this species, no research on the population dynamics such as age and growth rates has been conducted. The aim of this study was to determine the age, growth rate, and the relations between the fish size and the otolith size for C. erhani from the Menzelet Reservoir, Turkey.

Materials and methods. This study was carried out seasonally in the Menzelet Reservoir, between January and December 2012. Samples were collected using gill nets with mesh sizes ranging from 18 to 30 mm.

Results. A total of 134 otoliths obtained from Capoeta erhani that ranged from 15 to 33.8 cm TL were used in the analyses. The overall male : female ratio (M : F = 1.25 : 1.00) was biased in favour of males. The Mann-Whitney test revealed significant differences between sexes, regarding TL (P < 0.05). The calculated length-weight relations were W = 0.0097TL^sup 3.0015^ (r^sup 2^= 0.986) for females, W = 0.0054TL^sup 3.2011^ (r^sup 2^ = 0.975) for males and W = 0.0075TL^sup 3.0892^ (r^sup 2^ = 0.979) for all specimens. The von Bertalanffy growth models were L^sub ∞^ = 33.83 cm (TL), K = 0.964, t^sub 0^ = -0.573 for females; L^sub ∞^ = 32.02 cm (TL), K = 0.843, t^sub 0^ = -0.562 for males; L^sub ∞^ = 33.85 cm (TL), K = 0.821, t^sub 0^ = -0.482 for all specimens.

Conclusion. This study provides the first information on age, growth, and otolith morphometric parameters of Capoeta erhani. Determining the parameters of C. erhani age and growth rates will lead to the estimates of relevant parameters of population dynamics and to better understanding of the long-term changes of the stock sizes.

Keywords: growth rate, sagittal otolith, otolith morphometrics

(ProQuest: ... denotes formulae omitted.)

INTRODUCTION

Age information is the most important biological variable for estimating growth rates of fishes (Homauni et al. 2011). Determination of the age and growth parameters of fish populations is a very important issue for fisheries management. Fish age is usually estimated using the periodicity of the formation of increments in scales, otoliths, fin rays, and vertebrae (Campana 2001). Otoliths, however, are the structures that are the most preferred by fisheries scientists because of the precision of age estimates (Maceina and Sammons 2006, Cikes Kec and Zorica 2013).

The genus Capoeta Valenciennes, 1842 is distributed in southern China, northern India, Turkmenistan, Lake Aral, the Middle East, and Anatolia. These fishes inhabit gravel and stony zones of fast flowing rivers (Türkmen et al. 2002), but some species may also be found in lakes and springs (Turan et al. 2008). In the inland waters of Turkey there are five species and six subspecies of the genus Capoeta (see Türkmen et al. 2002, Elp and Sen 2009). Capoeta erhani Turan, Kottelat et Ekmekçi, 2008 was described fromthe Ceyhan River, Turkey (Turan et al. 2008). This species has an economic importance in commercial and recreational fisheries for the local people around the Ceyhan River. The knowledge of the biology of this species is very scarce. Despite its importance, no study on the population dynamics such as age and growth rates has been conducted.

The aim of this study was to determine the age, growth rate, and the relations between the fish size and the otolith size for Capoeta erhani from the Menzelet Reservoir, in the Ceyhan River drainage.

MATERIAL AND METHODS

This study was carried out seasonally in the Menzelet Reservoir, located at 37°43'N, 36°51'E and 700 m altitude (above sea level) in the Ceyhan River at the eastern Mediterranean region of Turkey, between January and December 2012. Samples were collected using gill nets with mesh sizes ranging from 18 to 30 mm. Total length (TL) of specimens was measured to the nearest 0.1 mm and the fish were weighed to the nearest 0.01 g. Sex determination was performed by direct observation of gonads. The sex ratio (number of males to each female; M : F) of the samples was analysed. The Mann-Whitney test was applied to test the existence of significant differences between sexes according to total length. The relation between total length and total weight was calculated for each sex separately using a power function

...

where: b is the regression coefficient and a is the regression constant.

Age and growth. Sagittal otoliths (leftand right) were removed, cleaned of adhering tissue, dried, and stored in plastic vials. From each pair, one otolith was randomly selected and immersed in plastic vial with glycerine solution for 1 month. Counts of rings in each otolith were blind-read by two readers, who did not know the fish length or the date of capture. All otoliths were read twice and final age estimates achieved when the same results were obtained from the two readers. A light microscope with objective lenses with nominal magnifications ranging from 0.5 × to 5.6 × were used for the counts.

Otolith length (OL), width (OW), and radius (OR) were measured to the nearest 0.001 mm below using Q Capture Imaging Software. OL was defined as the longest axis between the anterior and posterior otolith edge and OW as a distance from the dorsal to the ventral edge. OR was measured as the longest axis between the nucleus and posterior edge. The results were separated according to sex and statistically tested with t-test. The relation between the somatic growth and the otolith growth was investigated by linear regression.

Age was determined by counting the number of increments from the nucleus to the outer edge of the otolith (Fig. 1). The von Bertalanffy growth function was used to fit the length at age data using non-linear least squares parameter estimation (von Bertalanffy 1938);

...

where: TL is the fish length at age t (year), L∞ the theoretical asymptotic length, K the growth rate coefficient, and t0 the theoretical age when fish length is zero. Separate analyses were carried out for males, females and all specimens.

RESULTS

Length-weight relation. A total of 135 otoliths (75 males and 60 females) were processed for age counts, however, a single pair of sagittal otolith was unreadable. Thus, 134 otoliths obtained from Capoeta erhani that ranged from 15 to 33.8 cm TL were used in the analyses. Males were smaller than females, ranging from 15-32 cm and 15.2-33.8 cm TL, respectively (Fig. 2). The Mann-Whitney test revealed significant differences between sexes, regarding TL (U = 1778; P < 0.05). The overall male : female ratio (M : F = 1.25 : 1.00) was biased in favour of males.

The length-weight relations were calculated as, W = 0.0097TL3.0015 (r2 = 0.986) for females, W = 0.0054TL3.2011 (r2 = 0.975) for males, and W = 0.0075TL3.0892 (r2 = 0.979) for all specimens. Our data suggested that Capoeta erhani showed positive allometric growth (Fig. 3).

Age and growth. The total lengths of aged specimens ranged from 15 to 33.8 cm (Table 1). The age-classes in the otolith samples ranged from 0+ to 6+ years, while the samples were dominated by the 1+ and 3+ age-classes (36.6% and 32.8%, respectively). There were no difference between the age distributions of males and females, as the majority of females were 1+ (14.2%) and 3+ (13.4%) years old, while males were mostly the same 1+ (23.1%) and 3+ (19.4%) years old (Fig. 4). Otoliths of smaller individuals showed a regular growth pattern with clearly identifiable increments in the first six years and an increasing complexity afterwards (Fig. 4).

Sagittal otolith length, width, and radius were 2.31-4.59 mm, 1.90-3.59 mm, and 1.00-2.30 mm, respectively. The different otolith measurements (OL, OW, and OR) showed significant linear relations with the fish length (TL) (Table 2). The effect of sex on the otolith size (OL, OW, and OR) of C. erhani was not statistically significant (P > 0.05). The von Bertalanffy growth curves (Fig. 5) were fitted to the length at age data for both sexes separately. The estimated parameters of the equation were: L∞ = 33.83 cm (TL), K = 0.964, t0 = -0.573 for females; L∞ = 32.02 cm (TL), K = 0.843, t0 = -0.562 for males; L∞ = 33.85 cm(TL), K = 0.821, t0 = -0.482 for all specimens.

DISCUSSION

The largest Capoeta erhani reported in the literature as 28 cm standard length (SL), was caught in the Ceyhan River (Turan et al. 2008). In our study, the largest fish was 33.8 cm in total length and 412 g in total weight. The overall male : female ratio was found as 1.25 : 1.00 in favour of males. Maximum length of 32.0 cm TL for males and 33.8 cm TL for females were determined.

The exponent of the total length-weight relations showed that growth is positive allometric for males (b = 3.2011) and isometric for females (b = 3.0015). The b-values estimated from this study are closely similar from those found for Capoeta capoeta from Karasu Stream, Turkey (Elp and Sen 2009) and from Zernek Dam Lake (Sen et al. 2008), for C. fusca from eastern Iran (Patimar and Mohammadzadeh 2011), but were different from those found by Patimar and Farzi (2011) for C. trutta from western Iran, and for C. umbla from Lake Hazar, Turkey (Çoban et al. 2013). The variations in the exponents could be affected by ecological factors such as temperature, food supply, spawning conditions, and habitat characteristics within the year (Bagenal and Tesch 1978, Wootton 1992, Ismen 2005). Geographic locations and the different species belonging to the same genus can also possibly affect the value of "b".

The sagittal otoliths of the Capoeta erhani showed opaque and translucent bands, which could be used for age determination. These seasonal bands are generally associated with changes in water temperature. In the presently reported study, we determined that whole otoliths are suitable for age and growth studies for this species. In addition, this process was not time consuming.

In the presently reported study, one pair of the largest otoliths could not be read. The analysis of the other 134 otoliths revealed maximum age and length as 6+ years and 33.8 cm TL for females, and 6+ years and 32 cm TL for males, respectively. In the analysed samples, males were the dominant until the age 4+, but after then females were the dominant in the population (Fig. 4). In the wild populations the younger age-class is expected to be more abundant than the older ones (Cikes Kec and Zorica 2013). However, in our study, the 2+ age class specimens were very low. It might be explained by the characteristics of the gill nets. Since no data of the Capoeta erhani age and growth rates were available for comparison with this study, data on the different species of the genus Capoeta were used. Maximum ages of C. erhani are lower than those reported for the C. capoeta from Zernek Dam Lake Van, Turkey (Sen et al. 2008), for the C. trutta from Karakaya Dam Lake (Kalkan 2008), and for the C. umbla from the Lake Hazar, Elazig, Turkey (Çoban et al. 2013) and from the Askale region of the Karasu River, Turkey (Türkmen et al. 2002). Also, some of the other studies have been reported with the similar results of the maximum ages of C. erhani, for the C. capoeta from Karasu Stream, Turkey (Elp and Sen 2009) and for the C. trutta from the Meymeh River, western Iran (Patimar and Farzi 2011). The maximum age results of Capoeta erhani from this study differed from those of the other species of the Capoeta genus probably due to the different ageing methods (scales) or/and different species used.

Otolith size-fish size relations were significantly linear, demonstrating that otolith growth was proportional to the growth of fish for Capoeta erhani from the Menzelet Reservoir. This result showed that fish size could be estimated by using the otolith size. The similar results have been reported, for example, by Homauni et al. (2011) for clupeids and Amouei et al. (2013) for cyprinids.

The von Bertalanffy growth functions that were calculated forCapoeta erhanimales and females in this study showed that the female theoretical maximal length value (L∞ = 33.83 cm) was a little higher than themales (L∞ = 32.02 cm). Generally, the growth coefficient (K) is considered a genetic feature of a species, while L∞ is phenotypic and can be limited by environmental conditions (Cikes Kec and Zorica 2013). Growth parameters for all analysed material given in this paper were compared with the results of the other authors for Capoeta genus in Turkey (Table 3). The values of growth constant (K) obtained in this study differed from those estimated from the other researches (Türkmen et al. 2002, Alp et al. 2005, Erdogan unpublished*). Some species, most of them short-lived, have a high value of K (Sparre and Veneme 1998). In this study, K values were higher than the same values observed in literature, probably, due to obviously smaller maximum ages of fishes from the Menzelet Reservoir. The differences in the theoretical infinitive length value between regions can be attributed to the difference in the size of the largest individuals sampled in each area or the differences of species.

This study provides the first information on age, growth and otolith morphometric parameters of Capoeta erhani fromMenzelet Reservoir, Turkey. Determining the parameters of C. erhani age and growth rates will lead to the estimates of relevant parameters of population dynamics and to better understanding of the long-term changes of the stock sizes in this reservoir. Further investigations are necessary to compare age and growth rates of C. erhani from different areas.

Footnote

* Erdogan O. 1998. Aras Nehrinde yasayan Capoeta capoeta capoeta (Guldenstaedt, 1772) baliginin büyüme ve üreme özellikleri ile avlanma bölgesi suyunun bazi fiziko-kimyasal parametrelerinin arastirilmasi. [Growth and reproduction of Capoeta capoeta capoeta (Guldenstaedt, 1772) and investigation of some physico-chemical properties of the Aras River.] Doktora Tezi. [PhD Thesis.] Ataturk University, Fen Bilimleri Institution. Erzurum, Turkey. [In Turkish.]

References

REFERENCES

Alp A., Kara C., Büyükçapar H.M., Bülbül O. 2005. Age, growth and condition of Capoeta capoeta angorae Hanko, 1924 from the upper water systems of the River Ceyhan, Turkey. Turkish Journal of Veterinary and Animal Sciences 29 (3): 665-676.

Amouei F., Valinassab T., Haitov A. 2013. Age determination and morphological study using otoliths in Cyprinus carpio Linnaeus, 1758 in the southern Caspian Sea. Iranian Journal of Fisheries Sciences 12 (4): 749-756.

Bagenal T.B., Tesch F.W. 1978. Age and growth. Pp. 101-136. In: Bagenal T. (ed.) Methods for assessment of fish production in fresh waters. 3rd edn. IBP Handbook No. 3. Blackwell Scientific Publications, Oxford, London, Edinburgh, Melbourne.

Campana S.E. 2001. Accuracy, precision and quality control in age determination, including a review of the use and abuse of age validation methods. Journal of Fish Biology 59 (2): 197-242. DOI: 10.1006/jfbi.2001.1668

Çoban M.Z., Gündüz F., Demirol F., Örnekçi G.N., Karakaya G., Türkgülü I., Alp A. 2013. Population dynamics and stock assessment of Capoeta umbla (Heckel, 1843) in Lake Hazar, Elazig, Turkey. Turkish Journal of Fisheries and Aquatic Sciences 13 (2): 221-231. DOI: 10.4194/1303-2712-v13_2_04

Cikes Kec V., Zorica B. 2013. Length-weight relationship, age, growth and mortality of Atlantic chub mackerel Scomber colias in the Adriatic Sea. Journal of the Marine Biological Association of the United Kingdom 93 (Special Issue 02): 341-349. DOI: 10.1017/S0025315412000161

Elp M., Sen F. 2009. Biological properties of Capoeta capoeta (Guldenstaedt, 1773) population living in Karasu Stream (Van, Turkey). Journal of Animal and Veterinary Advances 8 (1): 139-142.

Homauni H., Valinassab T., Seifabadi S.J. 2011. Moghayeseye Khosoosiate Rikhtshenasi Otolithaye Sagitta Dar 10 Gouneh Az Shag Mahiane Khalij Fars Va Daryaye Oman. [Comparison of morphometric characteristics of otolith for ten clupeid species of the Persian Gulf.] Iranian Scientific Fisheries Journal 20 (2): 141-152. [In Persian.]

Ismen A. 2005. Age, growth and reproduction of the goldband goatfish, Upeneus moluccensis (Bleeker, 1855), in Iskenderun Bay, the eastern Mediterranean. Turkish Journal of Zoology 29 (4): 301-309.

Kalkan E. 2008. Growth and reproduction properties of Capoeta trutta (Heckel, 1843) in Karakaya Dam Lake. Turkish Journal of Zoology 32 (1): 1-10.

Maceina M.J., Sammons S.M. 2006. An evaluation of different structures to age freshwater fish from a northeastern US river. Fisheries Management and Ecology 13 (4): 237-242. DOI: 10.1111/j.1365-2400.2006.00497.x

Patimar R., Farzi S. 2011. Life history and other biological traits of the trout barb Capoeta trutta in the River Meymeh (western Iran). Folia Zoologica 60 (2): 153-158.

Patimar R., Mohammadzadeh B. 2011. On the biological characteristics of Capoeta fusca Nikolskii, 1897 in eastern Iran. Journal of Applied Ichthyology 27 (3): 873-878. DOI: 10.1111/j.1439-0426.2010.01572.x

Sen F., Elp M., Kankaya E. 2008. Growth and reproduction properties of Capoeta capoeta (Guldenstaedt, 1772) in Zernek dam lake, Van, Turkey. Journal of Animal and Veterinary Advances 7 (10): 1267-1272.

Sparre P., Veneme S.C. 1998. Introduction to tropical fish stock assessment. Part 1. Manual. FAO Fisheries Technical Paper No: 306.1 (Rev. 2).

Turan D., Kottelat M., Ekmekçi F.G. 2008. Capoeta erhani, a new species of cyprinid fish from Ceyhan River, Turkey (Teleostei: Cyprinidae). Ichthyological Exploration of Freshwaters 19 (3): 263-270.

Türkmen M., Erdogan O., Yildirim A., Akyurt I. 2002. Reproduction tactics, age and growth of Capoeta capoeta umbla Heckel 1843 from the Askale Region of the Karasu River, Turkey. Fisheries Research 54 (3): 317-328. DOI: 10.1016/s0165-7836(01)00266-1

von Bertalanffy L. 1938. A quantitative theory of organic growth (inquiries on growth laws II). Human Biology 10 (2): 181-213.

Wootton R.J. 1992. Constraints in the evolution of fish life histories. Netherlands Journal of Zoology 42 (2): 291-303. DOI: 10.1163/156854291X00342

Received: 4 March 2014

Accepted: 27 April 2014

Published electronically: 30 June 2014