Abstract

Doc number: 348

Abstract

Background: Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present study characterizes Sri Lankan An. barbirostris using mtDNA cytochrome oxidase subunit I (COI) and ribosomal RNA internal transcribed spacer 2 (ITS2) gene sequences.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries. Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.

Conclusions: Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l . form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

Details

Title
Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka
Author
Gajapathy, Kanapathy; Jude, Pavilupillai J; Goodacre, Sara L; Peiris, Lalanthika BS; Ramasamy, Ranjan; Surendran, Sinnathamby N
Pages
348
Publication year
2014
Publication date
2014
Publisher
BioMed Central
e-ISSN
1756-3305
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1756-3305-7-348
ProQuest document ID
1551506186
Copyright
© 2014 Gajapathy et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.