Abstract

Doc number: 559

Abstract

Background: Early and unambiguous detection of bovine tuberculosis (bTB), a significant disease of cattle worldwide, is necessary to control the spread of infection to other animals and humans. Current testing strategies are laborious, time consuming and heavily reliant on host responses that do not distinguish bTB from other mycobacteria. We report the presence of a pathogen signature, liparabinomannan (LAM), as a potential biomarker for bTB infection.

Findings: Fifty-five animals (uninfected [n = 33], bTb [n = 10] and exposed cases [n = 12]) from a well characterized bovine serum repository were screened for the presence of LAM using a commercially available ELISA. Analysis showed that LAM had a sensitivity of 100% and a specificity of 91.7% for bTB detection (bTB positive versus bTB exposed animals).

Conclusion: LAM detection easily separated bTB infected animals from bTB exposed and negative controls. We propose that pathogen related markers, such as LAM, should be included with current testing strategies as a battery diagnostic for bTB.

Details

Title
Mannosylated lipoarabinomannan in serum as a biomarker candidate for subclinical bovine tuberculosis
Author
Lamont, Elise A; Ribeiro-Lima, João; Waters, Wade Ray; Thacker, Tyler; Sreevatsan, Srinand
Pages
559
Publication year
2014
Publication date
2014
Publisher
BioMed Central
e-ISSN
17560500
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1756-0500-7-559
ProQuest document ID
1558338890
Copyright
© 2014 Lamont et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.