Abstract

Doc number: 68

Abstract

Background: Genetic methods for inducibly and reversibly inhibiting neuronal activity of specific neurons are critical for exploring the functions of neuronal circuits. The engineered human glycine receptor, called ivermectin (IVM)-gated silencing receptor (IVMR), has been shown to possess this ability in vitro .

Results: Here we generated a mouse line, in which the IVMR coding sequence was inserted into the ROSA26 locus downstream of a loxP-flanked STOP cassette. Specific Cre-mediated IVMR expression was revealed by mis-expression of Cre in the striatum and by crossing with several Cre lines. Behavioral alteration was observed in Rosa26-IVMR mice with unilateral striatal Cre expression after systemic administration of IVM, and it could be re-initiated when IVM was applied again. A dramatic reduction in neuron firing was recorded in IVM-treated free moving Rosa26-IVMR;Emx1-Cre mice, and neuronal excitability was reduced within minutes as shown by recording in brain slice.

Conclusion: This Rosa26-IVMR mouse line provides a powerful tool for exploring selective circuit functions in freely behaving mice.

Details

Title
A mouse line for inducible and reversible silencing of specific neurons
Author
Hu, Ling; Lan, Wei; Guo, Hao; Chai, Guo-Dong; Huang, Kun; Zhang, Ling; Huang, Ying; Chen, Xue-Feng; Zhang, Lei; Song, Ning-Ning; Chen, Ling; Lang, Bing; Wang, Yun; Wang, Qing-Xiu; Zhang, Jin-Bao; McCaig, Collin; Xu, Lin; Ding, Yu-Qiang
Publication year
2014
Publication date
2014
Publisher
BioMed Central
e-ISSN
1756-6606
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s13041-014-0068-8
ProQuest document ID
1565879054
Copyright
© 2014 Hu et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.