Abstract

Doc number: 49

Abstract

Background: Chitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).

Materials and methods: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E .) coli , Pseudomonas (P .) aeruginosa , and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.

Results: The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli , P. aeruginosa , and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.

Conclusions: Our results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

Details

Title
Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites
Author
Nguyen, Vinh Quang; Ishihara, Masayuki; Kinoda, Jun; Hattori, Hidemi; Nakamura, Shingo; Ono, Takeshi; Miyahira, Yasushi; Matsui, Takemi
Publication year
2014
Publication date
2014
Publisher
BioMed Central
e-ISSN
14773155
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/s12951-014-0049-1
ProQuest document ID
1635672670
Copyright
© 2014 Nguyen et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.