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ARTICLE

Received 15 Jul 2014 | Accepted 26 Feb 2015 | Published 16 Apr 2015

DOI: 10.1038/ncomms7780

Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity

Toshiaki Fukushima1,2, Hidehito Yoshihara1,3, Haruka Furuta1, Hiroyasu Kamei1, Fumihiko Hakuno1, Jing Luan4, Cunming Duan5, Yasushi Saeki3, Keiji Tanaka3, Shun-Ichiro Iemura6, Tohru Natsume7, Kazuhiro Chida1, Yusuke Nakatsu2, Hideaki Kamata2, Tomoichiro Asano2 & Shin-Ichiro Takahashi1

Insulin-like growth factors (IGFs) induce proliferation of various cell types and play important roles in somatic growth and cancer development. Phosphorylation of insulin receptor substrate (IRS)-1/2 by IGF-I receptor tyrosine kinase is essential for IGF action. Here we identify Nedd4 as an IRS-2 ubiquitin ligase. Nedd4 monoubiquitinates IRS-2, which promotes its association with Epsin1, a ubiquitin-binding protein. Nedd4 recruits IRS-2 to the membrane, probably through promoting Epsin1 binding, and enhances IGF-I receptor-induced IRS-2 tyrosine phosphorylation. In thyroid FRTL-5 cells, activation of the cyclic AMP pathway increases the association of Nedd4 with IRS-2, thereby enhancing IRS-2-mediated signalling and cell proliferation induced by IGF-I. The Nedd4 and IRS-2 association is also required for maximal activation of IGF-I signalling and cell proliferation in prostate cancer PC-3 cells. Nedd4 overexpression accelerates zebrash embryonic growth through IRS-2 in vivo. We conclude that Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity.

1 Department of Animal Sciences and Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. 2 Department of Medical Science, Graduate School of Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima City, Hiroshima 734-8553, Japan. 3 Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan. 4 Key Laboratory of Marine Drugs, Chinese Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China. 5 Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, Michigan 48109, USA. 6 Translational Research Center, Fukushima Medical University, 11-25 Sakaemachi, Fukushima City, Fukushima 960-8031, Japan.

7 Molecular Proling Research Center for Drug Discovery (molprof), National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan. Correspondence and requests for materials should be addressed to S.-I.T. (email: mailto:[email protected]

Web End [email protected] ).

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ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/ncomms7780

Insulin-like growth factor (IGF)-I and IGF-II induce cell proliferation, differentiation, survival and migration in many cell types1. In vivo, IGFs play important roles in prenatal and

postnatal development2. Reduced IGF activities cause growth retardation, whereas elevated IGF activities cause overgrowth and cancer development3. The growth-promoting activities of IGFs or their homologues are evolutionarily conserved in metazoans4. IGFs bind to the IGF-I receptor (IGF-IR) in the plasma membrane and induce the activation of its intrinsic tyrosine kinase and auto-phosphorylation. The activated IGF-IR phosphorylates intracellular substrates including insulin receptor substrate (IRS)-1 and IRS-2. Phosphotyrosyl IRSs are in turn recognized by SH2 domain-containing proteins such as the p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K). These events trigger activation of downstream signalling pathways, leading to various biological actions5.

IGF activities are often modulated by other hormonal factors6,7. IRSs serve as signal nodes on which IGF signals and other signals converge. Indeed, IGF-induced IRS tyrosine phosphorylation is potentiated by other hormonal stimuli, leading to amplication of IGF activities810. In addition, oncogenic pathways frequently cause excess IRS-mediated signalling, which contribute to tumour growth and metastasis11. We have shown that IRSs form high-molecular-mass complexes (IRSomes) and different complexes are formed depending on IRS isoform, cell types or hormone stimulation12. Subsequent study showed that IRS-associated proteins potentially modulate the availability of IRSs to receptor tyrosine kinases12, prompting us to identify IRS-associated proteins and to understand their functions.

In this study, we identied HECT-type ubiquitin ligase Nedd4 as a novel IRS-2-associated protein. Nedd4 is expressed in a variety of tissues and regulates the trafcking and/or degradation of its substrate proteins13,14. IGF-IR is reported to be a putative Nedd4 substrate, but the effects of Nedd4 on IGF-IR are controversial15,16. Nedd4 knockout mice show fetal growth retardation and perinatal lethality16, indicating a crucial role for Nedd4 in embryonic growth. Nedd4 is overexpressed in several types of cancers and functions as an oncogenic protein1721. We provide multiple lines of evidence, suggesting that Nedd4 conjugates monoubiquitin to IRS-2 and recruits IRS-2 to the plasma membrane, thereby enhancing IRS-2 tyrosine phosphorylation by IGF-IR and IGF-I mitogenic activity.

ResultsNedd4 is an IRS-2-associated protein. We previously reported that pretreatment of thyroid FRTL-5 cells with thyroid stimulating hormone (TSH) or other cAMP-generating reagents enhances IGF-I-induced IRS-2 tyrosine phosphorylation and increased cell proliferation10,22,23. Our study also suggested that some IRS-2-associated protein(s) might increase the availability of IRS-2 to the IGF-IR tyrosine kinase12. Using co-immunoprecipitation followed by matrix-assisted laser desorption/ionizationtime-of-ight mass spectrometry (MS) analysis, we identied Nedd4 as an IRS-2-associated protein in FRTL-5 cells treated with dibutyryl cAMP (Supplementary Fig. 1a,b). This association was conrmed by coimmunoprecipitation and immunoblotting (Supplementary Fig. 1c). Co-immunoprecipitation analysis using HEK293 cells expressing exogenous IRS-2 together with Nedd4 or Nedd4-2, a related protein in the Nedd4 family13, revealed that IRS-2 is associated only with Nedd4 (Supplementary Fig. 1d).

Next, we generated a series of IRS-2 deletion mutants and performed pull-down analyses. The results revealed that IRS-2 amino acids (aa) 1134 (containing a PH domain) as well as 137329 (containing a PTB domain) are critical for its binding to

Nedd4 (Fig. 1a). Similar experiments using Nedd4 deletion mutants demonstrated that Nedd4 1226 (containing a C2 domain) is important (Fig. 1b). Nedd4 1226 can bind to IRS-2 1134 as well as 137329 in vitro (Supplementary Fig. 2a).

We further investigated the IRS-2-binding site in Nedd4 in greater detail. The Nedd4 C2 domain can bind intramolecularly to the HECT domain24. Overexpression of HECT domain in HEK293 cells decreased the association of Nedd4 1551 with IRS-2 (Fig. 1c), indicating that the Nedd4 HECT domain and IRS-2 may competitively bind to the Nedd4 C2 domain. This raised the possibility that the HECT domain and IRS-2 may bind to an overlapping site in the C2 domain. To test this idea, we modelled the Nedd4 intramolecular binding sites based on the reported structure of Smurf2, a Nedd4-type ubiquitin ligase24. Based on the modelling results (Supplementary Fig. 2b), several mutants were generated. Mutation of Ile95 and Leu96 to Ala, or Ser126 and Leu127 to Ala inhibited the association of Nedd4 1502 with Nedd4 503887 (Supplementary Fig. 2c). These data suggest that these residues are part of the intramolecular binding sites. These mutations also inhibited the association of Nedd4 with IRS-2 (Fig. 1d), indicating their roles in IRS-2 binding.

Nedd4 induces IRS-2 monoubiquitination. We investigated whether Nedd4 ubiquitinates IRS-2 using a cell-free ubiquitination assay. The results showed that Nedd4 caused a prominent upward mobility shift of IRS-2, a typical pattern of protein ubiquitination (Fig. 2a). Furthermore, overexpression of Nedd4 in HEK293/HEK293T cells increased the ubiquitination signal in IRS-2 immunoprecipitates (Fig. 2b). The signal was observed when proteins that bound to IRS-2 (for example, Nedd4) were removed by denaturing the samples before immunoprecipitation (Fig. 2b), indicating that the observed signal arose from ubiquitinated IRS-2 itself. The detected signal was 2030 kDa larger than the intact IRS-2, indicating the conjugation of a small number of ubiquitin molecules. The exact number was unclear, as the IRS-2 mobility may be changed by other posttranslational modications in addition to ubiquitination. Nedd4-induced IRS-2 ubiquitination was not affected by IGF-I stimulation (Supplementary Fig. 3a). Nedd4 overexpression also induced IGF-IR ubiquitination as previously reported15, but it did not induce IRS-1 ubiquitination (Supplementary Fig. 3a).

Two antibodies were used to examine whether Nedd4-induced IRS-2 ubiquitination is monoubiquitination or polyubiquitination. Anti-ubiquitin antibody P4D1 can recognize both monoubiquitin and polyubiquitin chains, whereas FK1 only recognizes polyubiquitin chains25. IRS-2 ubiquitination was detected only with P4D1 (Fig. 2c). We also compared Nedd4-dependent IRS-2 ubiquitination in cells overexpressing haemagglutinin (HA)-tagged ubiquitin (HA-Ub) or modied HA-Ub in which all Lys residues were replaced with Arg (HA-UbK0). The molecular weight of IRS-2 conjugated with HA-UbK0 was similar to that with HA-Ub (Fig. 2d). IRS-2 conjugated with HA-UbK0 was less than that with HA-Ub, possibly due to low expression level of HA-UbK0 in our experimental system. IRS-2 ubiquitination was also detected when cells were overexpressing Nedd4Y592A or Nedd4F694A that can conjugate single ubiquitin to substrates but lack ubiquitin chain elongation activity26 (Fig. 2e). Taken together, these results indicated that IRS-2 ubiquitination by Nedd4 occurs as monoubiquitination at single or multiple lysine residues.

To map the IRS-2 ubiquitination sites, we generated a series of IRS-2 mutants in which all Lys residues in a dened region are replaced with Arg (Fig. 2f). Mutation of all Lys residues in the C1C3 region (C1C3 KR) abolished IRS-2 ubiquitination (Fig. 2g,h), indicating that IRS-2 ubiquitination sites are located

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NATURE COMMUNICATIONS | DOI: 10.1038/ncomms7780 ARTICLE

in this region. Mutation in the C3 region largely decreased IRS-2 ubiquitination (Fig. 2g,h), indicating that Lys residues in the C3 region are more important. Further analysis using site-directed mutagenesis targeting Lys residues in the C3 region revealed that Lys1331 is the most frequently ubiquitinated site (Fig. 2g,h). In parallel experiments, we used liquid chromatographytandem mass spectrometry (LC-MS/MS) to identify ubiquitination sites in HEK293T cells overexpressing Nedd4. We detected several IRS-2 peptides containing the ubiquitin remnant motif (K-e-GG)

marking ubiquitinated proteins after trypsinolysis (Supplementary Fig. 3b). Importantly, the peptide containing Lys1331 with the ubiquitin remnant was detected (Supplementary Fig. 3c). Quantitative MS analysis revealed that the peptide containing Lys1331 with ubiquitin remnant was intensely increased by Nedd4 overexpression (Fig. 2I and Supplementary Fig. 3d,e).

IRS-2 monoubiquitination enhances IGF-I signalling. We investigated the effects of Nedd4 overexpression on IGF-I

signalling in HEK293 cells. Although Nedd4 overexpression did not affect the levels of IGF-IR or IGF-I-induced IGF-IR tyrosine phosphorylation (Fig. 3a,b and Supplementary Fig. 4a), it enhanced IGF-I-induced IRS-2 tyrosine phosphorylation and increased the amounts of PI3K bound to IRS-2 (Fig. 3a,b). Time-course experiments showed that Nedd4 increased the magnitude of IGF-I-induced IRS-2 tyrosine phosphorylation at 1 and 3 min (Fig. 3c). In contrast, Nedd4 overexpression resulted in modest decreases in the total IRS-1 levels and IGF-I-induced IRS-1 tyrosine phosphorylation (Supplementary Fig. 4b,c). Nedd4 overexpression did not affect the amounts of PI3K bound to IRS-1 (Supplementary Fig. 4b).

We next investigated the underlying mechanism. In mouse embryonic broblasts, Nedd4 was reported to maintain cell-surface IGF-IR levels by suppressing the abundance of the adaptor protein Grb10 (ref. 16). However, Nedd4 overexpression neither affected cell-surface IGF-IR levels nor Grb10 levels in HEK293 cells (Supplementary Fig. 4c,d). The Nedd4-induced increase in IRS-2 tyrosine phosphorylation

IB: myc

FLAG

IRS-2

137-1338

330-1338

1-545

137-

1-329 1-134 329

IRS-2 deletion mutants (FLAG-tagged)

PH PTB

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137-329

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GST-Nedd4

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GST

GST-Nedd4

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GST

GST-Nedd4

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GST

GST-Nedd4

137-1338 330-1338

210 117

98 55

(kDa)

37 29

1-545

1-329 1-134

210

117

55 (kDa)

Nedd4 binding regions

C2 WW HECT

1-887(full length)

IB:FLAG IB: FLAG

Nedd4 deletion mutants (GST-tagged)

IRS-2 binding region

GST

1-887

1-226

227-551

552-887

227-887

227-551*

1-551

1-226 227-551 552-887 227-887

1-551

210

myc-IRS-2

210 117

(kDa)

227-551

GST-Nedd4 mutants

55 CBB stain

(*227-551, no production)

myc-IRS-2-FLAG

IP: FLAG, IB: myc

IB: Nedd4

myc-IRS-2-FLAG myc-Nedd4 1-551 (HECT)

myc-Nedd4 552-887 (HECT)

+ + + + +

+ + + +

0.25 0.5 1 1 1

IP: FLAG, IB: myc

IB: myc

(g)

myc-Nedd4

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196

91 35

(kDa)

IRS-2

Nedd4 HECT binding

Nedd4 HECT

I95A, L96A

S126A, L127A

E541A

196

124

(kDa)

IRS-2

Nedd4

Nedd4 HECT

Nedd4 binding

124

Figure 1 | Nedd4 is an IRS-2-associated protein. (a,b) Determination of the binding regions. HEK293T cells overexpressing the indicated FLAG-tagged IRS-2 deletion mutants (a) or myc-IRS-2 (b) were serum starved and cell lysates were subjected to pull-down analysis using GST-Nedd4 or its deletion mutants shown in b. The purity of GST-tagged proteins was validated by SDSPAGE and CBB stain. Input, B1/100 volume of HEK293T lysates.

*GST-Nedd4 D227551 was not successfully expressed. (c,d) Effects of Nedd4 HECT domain (c) and various Nedd4 mutants (d) on the IRS-2Nedd4 C2 domain interaction. HEK293 cells overexpressing indicated proteins were serum starved and cell lysates were subjected to immunoprecipitation and immunoblotting using the indicated antibodies.

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ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/ncomms7780

was reduced by the deletion of the Nedd4 amino-terminal region (IRS-2-binding region; Supplementary Fig. 4e) and was abolished by the substitution of Nedd4 Cys854 with Ser, which inactivates Nedd4 ubiquitin ligase activity14 (Fig. 3a,b). These results suggest that the Nedd4-induced IRS-2

ubiquitination leads to the enhancement of IRS-2 tyrosine phosphorylation.

To elucidate whether monoubiquitination of IRS-2 is required for the increased IGF signalling, we used several IRS-2 ubiquitination site mutants. Both IRS-2 C1-C3 KR mutation

Purified mono-Ub

Nedd4

Denaturing

+ +

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GST-Nedd4

GST

IRS-2

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IB: P4D1 FK1

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119

Nedd4

IRS-2-FLAG

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IRS-2-FLAG

FLAG-IRS-2

IB: FLAG

IB: Nedd4

IB: Ub (P4D1)

IB: IRS-2

199

116

IB: P4D1 FK1 FLAG FK1

P4D1

IB: Nedd4

IRS-2-FLAG-(Ub)n

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119 (kDa)

GST-Nedd4

119

IB: Nedd4

116

(kDa)

IP: IRS-2

(kDa)

HA-Ub

+ +

Defined Regions

PTB

IPK

KRLB

Nedd4

IRS-2-FLAG

+ +

+ + + +

myc-Nedd4

Y592A

F694A

IRS-2-FLAG

+ + + +

192

118 91

(kDa)

Nedd4

196

124

196

124 124

IRS-2-FLAG-(Ub)n

Nonspecific staining of IRS-2-FLAG

HA-Ub monomer

196

(kDa)

IRS-2-FLAG-(Ub)n

119

IB: Ub (P4D1)

IB: FLAG

IP: FLAG

196

119

192

8.7

IRS-2-FLAG

IB: HA

IB: FLAG

IP: FLAG

IB: Nedd4

118

IB: myc

IB: HA

KRLB-C3 KR

IPK-C3 KR

PTB-C3 KR

All KR

C1-C3 KR

C3 KR

1134, 1170, 1184 KR

K1337R

K1281R

K1331R

C1-C3 KR

+ + + + + + +

IRS-2-FLAG-(Ub)n

IP: FLAG IB: Ub

IP: FLAG IB: FLAG

IB: Nedd4

(kDa)

1.2

Ub-IRS-2

* *

(fold of control)

0 +

Nedd4 + +

C1-C3 KR

C3 KR

AUC of fragment ions

(fold of control)

1.0

0.4

0.6

0.8

6 Nedd4 +Nedd4

0.2

+ + + + +

K1281R

K1331R

K1337R

1134, 1170, 1184 KR

IRS-2

0 K1106

K1281

K1134

K1331

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NATURE COMMUNICATIONS | DOI: 10.1038/ncomms7780 ARTICLE

Nedd4 enhances IGF-I signalling in thyrocytes. Prolonged pretreatment of nontransformed thyroid FRTL-5 cells with TSH or other cAMP-generating reagents is known to enhance IGF-I-dependent IRS-2 tyrosine phosphorylation and DNA synthesis10,22,23. As shown in Fig. 5a and Supplementary Fig. 6a, dibutyryl cAMP treatment of FRTL-5 cells increased the association of endogenous Nedd4 with IRS-2. Pull-down analysis using GST-IRS-2 (aa 1329) or GST-Nedd4 (aa 1226) showed that cAMP treatment increases the afnity of Nedd4 for IRS-2 (Fig. 5b). In comparison, no association of IRS-2 with Nedd4-2 was detected (Fig. 5a). It should be noted that cAMP treatment changed the IRS-2 electrophoretic mobility (Fig. 5a and subsequent gures). Our previous study showed that cAMP treatment does not change IRS-2 levels and the cAMP treatment-induced IRS-2 mobility shift is due to its Ser/Thr phosphorylation10. Although Nedd4 was reported to interact with IGF-IR previously in other cell types15, our analysis indicated that IGF-IR was not co-immunoprecipitated with IRS-2 (Supplementary Fig. 6b), indicating that Nedd4 associates with IGF-IR and IRS-2, independently. Next, we tested the effects of cAMP treatment on IRS-2 ubiquitination. Immunoblotting analysis with P4D1 and FK1 revealed that cAMP treatment increased monoubiquitination of IRS-2 (Fig. 5c). Subcellular fractionation showed that cAMP treatment markedly increased the amounts of IRS-2 in the membrane fraction (Fig. 5d).

We then studied the role of Nedd4 in IGF-I signalling and mitogenic activity in FRTL-5 cells. Nedd4 knockdown neither affected IGF-I-induced IGF-IR tyrosine phosphorylation (Fig. 5e,f) nor Grb10 levels (Supplementary Fig. 6c). cAMP pretreatment enhanced IGF-I-induced IRS-2 tyrosine phosphor-ylation and this was suppressed by Nedd4 knockdown (Fig. 5e,f) but not by Nedd4-2 knockdown (Supplementary Fig. 6d). cAMP pretreatment enhanced IGF-I-induced DNA synthesis and this was abolished by Nedd4 knockdown (Fig. 5g). These data indicated that Nedd4 is required for cAMP-dependent enhancement of IGF-I signalling and mitogenic activity.

Nedd4 enhances IGF-I signalling in prostate cancer cells. We also investigated the possible role of Nedd4 in IGF-I action in prostate cancer cells. We conrmed that Nedd4 associated with IRS-2 in prostate cancer DU145 cells and PC-3 cells (Fig. 6a).Nedd4 knockdown did not affect IGF-I-induced IGF-IR tyrosine phosphorylation in PC-3 cells, whereas it decreased IRS-2 tyrosine phosphorylation and the amounts of PI3K bound to IRS-2 (Fig. 6b). Nedd4-2 was not detected in these cells (Supplementary Fig. 7). Epsin1 knockdown also decreased IGF-I-induced IRS-2 tyrosine phosphorylation (Fig. 6c), supporting our conclusion that Epsin1 mediates Nedd4-induced enhancement of IGF-I signalling.

Figure 2 | Nedd4 induces IRS-2 monoubiquitination. (a) Cell-free ubiquitination assay results. HEK293T cells overexpressing FLAG-IRS-2 or FLAG were serum starved and lysed. Immunoprecipitates adsorbed on anti-FLAG antibody-conjugated beads were subjected to cell-free ubiquitination assay using GST-Nedd4 or GST. (be) IRS-2 ubiquitination by Nedd4 overexpression. HEK293 cells (b) or HEK293T cells expressing the indicated proteins (ce) were serum starved. A portion of the cell lysate was mixed with 2% SDS, boiled for 5 min and diluted. These denatured samples (indicated lanes in b and all immunoprecipitates in ce) and native samples were subjected to immunoprecipitation and immunoblotting as indicated. In c, puried monoubiquitin was subjected to immunoblotting, to conrm that FK1 antibody does not recognize monoubiquitin (upper panel). UbK0 is an ubiquitin mutant in which all Lys residues were substituted to Arg. Nedd4Y592A/F694A is a mutant lacking ubiquitin chain-elongation activity. (f) Location of Lys

residues in IRS-2. IPK, intermediate region anking the PTB domain and the KRLB region; KRLB, kinase regulatory loop-binding region; C1-3, C-terminal region 13. (g,h) Effects of the indicated Lys-Arg substitution in IRS-2 on its ubiquitination by Need4. HEK293Tcells overexpressing indicated proteins were serum starved. Cell lysates were denatured and analysed by immunoprecipitation and immunoblotting as indicated (g). Densitometric analyses were performed. Ubiquitination levels of IRS-2 were normalized to its protein levels, and the meanss.d. of four independent experiments were shownin h. *Signicant difference (Po0.05, one-way analysis of variance followed by TukeyKramer test). (i) Quantitative MS analysis results. IRS-2 immunoprecipitates derived from HEK293Tcells expressing IRS-2 (-Nedd4) or IRS-2 and Nedd4 ( Nedd4) were treated with trypsin and Asp-N. Peptides

with ubiquitin remnant motif were enriched and subjected to LC-MS/MS analysis. The graph shows meanss.d. of three independent experiments. *Signicant difference from control (Po0.05, Students t-test).

and K1331R mutation suppressed Nedd4-dependent increases in IRS-2 tyrosine phosphorylation (Fig. 3d), indicating that monoubiquitination at IRS-2 Lys1331 is required for Nedd4-induced enhancement of IRS-2 tyrosine phosphorylation. As Lys1331 is near the IRS-2 carboxy terminus (Fig. 2f), we also generated IRS-2 fused with monoubiquitin at the C terminus. When cells overexpressing this chimeric protein were stimulated with IGF-I, it was tyrosine phosphorylated and bound to PI3K more intensely than intact IRS-2 (Fig. 3e). Therefore, fusion of a single ubiquitin molecule to IRS-2 is sufcient to enhance its tyrosine phosphor-ylation in response to IGF-I.

Ubiquitin is recognized by a number of ubiquitin-binding proteins. These proteins target the ubiquitinated proteins to proteasome, sorting machinery or de-ubiquitinating enzymes27. Ile44 of ubiquitin has been shown to be crucial for its interaction with ubiquitin-binding proteins27. When the Ile44 in the IRS-2-ubiquitin chimeric protein was substituted to Ala, Nedd4-dependent increases in IRS-2 tyrosine phosphorylation was suppressed (Fig. 3e), suggesting that recognition of IRS-2 by ubiquitin-binding protein(s) is required for the Nedd4-dependent increases in IRS-2 tyrosine phosphorylation.

Epsin1 mediates the Nedd4 effects on IGF-I signalling. To identify proteins that recognize ubiquitinated IRS-2, we studied several ubiquitin-binding proteins in the endocytosis machinery, including Eps15, Eps15R, Epsin1, HRS and TSG101 (ref. 28). Among them, the association of Epsin1 with IRS-2 was increased by overexpression of Nedd4 (Fig. 4a) and the IRS-2-ubiquitin chimeric protein (Supplementary Fig. 5a). Nedd4 overexpression did not affect the association of the other ubiquitin-binding proteins with IRS-2 (Supplementary Fig. 5b). The association of IRS-2 with Epsin1 was suppressed by the mutation of IRS-2 ubiquitination sites (Fig. 4b) and by the deletion of Epsin1 ubiquitin-interacting motifs (UIMs)29 (Fig. 4b and Supplementary Fig. 5c). These results indicate that Epsin1 UIMs bind to the ubiquitin conjugated on IRS-2. Further analysis revealed that Epsin1 knockdown suppressed the effect of Nedd4 overexpression on IRS-2 tyrosine phosphorylation, while it had no effect on IGF-IR tyrosine phosphorylation (Fig. 4c). These results indicate that ubiquitinated IRS-2 is bound to Epsin1 and this molecular interaction is required for the enhanced IRS-2 tyrosine phosphorylation in response to IGF-I.

Epsin1 is localized at the plasma membrane, especially at sites where clathrin-coated pits will be formed30. Subcellular fractionation analysis showed that Nedd4 overexpression increased IRS-2 levels in the membrane fraction when cells were stimulated with IGF-I (Fig. 4d). These data suggested that Nedd4 recruits IRS-2 to the plasma membrane, where IRS-2 is effectively phosphorylated by the IGF-IR tyrosine kinase.

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ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/ncomms7780

2.5

Nedd4 Nedd4C854S

IB: pTyr

Nedd4 * *

Nedd4C854S

+ +

2.0

IGF-I 119

200

119

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(kDa)

IB: IGF-IR

IB: p85 PI3K

IB: IRS-2

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(fold of control)

1.5

1.0

0.5

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IGF-IR

pIGF-IR

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pIRS-2

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to IRS-2

(min)

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IB: IGF-IR

IB: IRS-2

IB: pTyr

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Nedd4

IGF-I 1 3 10 0 1 3 10

200

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119

IB: IRS-2

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IRS-2 Ub/UbI44A

IRS-2 IRS-2-Ub IRS-2-UbI44A

IRS-2

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IRS-2-Ub

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200

pIRS-2

IGF-I

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0 pIRS-2

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IP: IRS-2

Figure 3 | Need4 enhances IGF-I signalling by regulating IRS-2 ubiquitination. (ac) Effects of Nedd4 overexpression on IGF-I signalling. HEK293 cells overexpressing Nedd4 or Nedd4C854S (a ubiquitin ligase inactive mutant) together with IRS-2 were serum starved, followed by IGF-I stimulation (100 ng ml 1) for 1 min (a) or the indicated durations (c). Lysates were subjected to immunoprecipitation and immunoblotting using the indicated antibodies. The results of a were quantied by densitometric analysis. IGF-I-induced tyrosine phosphorylation levels of IGF-IRb and IRS-2 were normalized to their protein levels in immunoprecipitates and p85 PI3K bound to IRS-2 was normalized to IRS-2 levels in immunoprecipitates. The meanss.d. of three independent experiments are shown in b. *Signicant difference from control (Po0.05, one-way analysis of variance (ANOVA) followed by TukeyKramer test). (d,e) Effects of the expression of IRS-2 ubiquitination site mutants (d) and an IRS-2 -ubiquitin chimeric protein (e) on IRS-2 tyrosine phosphorylation.

HEK293 cells overexpressing indicated proteins were subjected to experiments similar to a. UbI44A, ubiquitin unable to interact with ubiquitin-binding domains. IGF-I-induced tyrosine phosphorylation levels of IRS-2 and PI3K bound to IRS-2 were normalized to IRS-2 protein levels in immunoprecipitates. Meanss.d. of six (d) or three (e) independent experiments are shown. *Signicant difference from control (Po0.05, one-way ANOVA followed by

TukeyKramer test); NS, not signicant.

Cultured PC-3 cells secrete IGF-I and -II into media, which stimulates their proliferation31,32. We observed that IGF-I stimulation increased DNA synthesis in PC-3 cells and this was decreased below the basal levels by Nedd4 knockdown as well as by IRS-2 knockdown (Fig. 6d), indicating that both Nedd4 and IRS-2 are necessary for their proliferation in response to endogenously secreted IGFs and exogenously added IGF-I. Together, these data indicate the requirement of the Nedd4-IRS-2

complex for maximum IGF-I signalling as well as mitogenic activity in PC-3 cells.

Nedd4 accelerates zebrash embryonic growth through IRS-2. To elucidate Nedd4 functions in an in vivo setting, we used the zebrash embryo model. The IGF signalling pathway is highly conserved and previous studies suggest that IGFs regulate

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DPW motifs (AP-2 binding) and Clathrin binding motifs

IRS-2-

FLAG

ENTH domain

3x UIMs Epsin1

+ + +

Nedd4

WT C854S

NPF motifs(EH domain binding)

IB: FLAG

IB: Epsin1

196

UIM

Epsin1-FLAG

myc-IRS-2 C1-C3KR

myc-IRS-2 K1331R

myc-IRS-2

Nedd4

IRS-2

IP: FLAG

IB: Nedd4

+ + +

119

+ + + +

(kDa)

Epsin1

Epsin1UIM

192

Control

siEpsin1

IRS-2 binding

IB: myc

IRS-2-FLAG Nedd4

IGF-I

pIGF-IR

IP: IGF-IR

IP: FLAG

+ + + + + +

IP: FLAG

IB: FLAG

IB: Nedd4

+ + +

+ + + +

192

118

124

IB: myc

IB: IGF-IR

IB: pTyr

IB: IRS-2

IB: pTyr

124

(kDa)

Membrane

Cytosol

pIRS-2

196

124

(kDa)

Nedd4

IGF-I

+ + + +

IB: Nedd4

200

IB: IRS-2

109

IB: Nedd4

88 196

109

IB: IGF-IR

IB: HSP90

90 (kDa)

2.5

2.0

1.5

1.0

0.5

Nedd4

IGF-I

Control siEpsin1

2.0

1.5

1.0

0.5

pIRS-2

(fold of control)

Membrane IRS-2/

cytosol IRS-2

(fold of control)

IGF-I

Nedd4

0 +

+ +

IB: Epsin1

IB: Eps15

+ + + +

+ +

Figure 4 | Epsin1 mediates the Nedd4 effect on IGF-I signalling. (a) Effects of Nedd4 overexpression on the IRS-2 and Epsin1 interaction. HEK293T cells overexpressing indicated proteins were serum starved. Lysates were subjected to immunoprecipitation and immunoblotting as indicated. (b) Effects of IRS-2 ubiquitination site mutation or deletion of Epsin1 UIM motif. Epsin1 structure is shown in the upper panel. ENTH, Epsin N-terminal homology; UIM, ubiquitin-interacting motif; DPW, Asp-Pro-Trp; AP-2, adaptor protein-2; NPF, Asn-Pro-Phe; EH, Eps15 homology. HEK293T cells overexpressing indicated proteins were serum starved. Lysates were subjected to immunoprecipitation and immunoblotting as indicated. (c) Role of Epsin1 in IGF-I-induced IRS-2 tyrosine phosphorylation. HEK293 cells transfected with indicated plasmids and/or siRNAs were serum starved and then treated with 100 ng ml 1 IGF-I for 1 min. Cell lysates were subjected to immunoprecipitation and immunoblotting. Densitometric analyses of cells with IGF-I stimulation were performed and tyrosine phosphorylation levels of IRS-2 were normalized to IRS-2 protein levels in immunoprecipitates. The meanss.d. of three independent experiments are shown in the graph. *Signicant difference (Po0.05, one-way analysis of variance (ANOVA) followed by TukeyKramer test). (d) Regulation of IRS-2 subcellular localization by Nedd4. HEK293 cells overexpressing Nedd4 and IRS-2 were serum starved, followed by IGF-I stimulation (100 ng ml 1, 1 min).

Cell lysates were fractionated into membrane/cytosol fractions, followed by immunoblotting using the indicated antibodies. Densitometric analyses were performed and the ratio of IRS-2 in the membrane fraction to IRS-2 in the cytosol fraction was calculated. The graph shows the means.d. of the densitometric analysis of six independent experiments. *Signicant difference (Po0.05, one-way ANOVA followed by TukeyKramer test).

zebrash embryonic growth by promoting cell proliferation and survival, without changing developmental patterning33,34. As shown in Fig. 7ac, Nedd4 overexpression increased zebrash embryo growth (body length) and developmental timing (somite number) without major organ loss or patterning abnormalities. These biological actions of Nedd4 were abolished by knocking down Irs2, using morpholino antisense oligos (MO) (Fig. 7ac). Specic translation block with Irs2 MO and Nedd4 overexpression were conrmed (Supplementary Fig. 8a-c). Taken together, these data indicate that Nedd4 accelerates zebrash embryonic growth through Irs2.

DiscussionIn this study, we identied Nedd4 as a novel IRS-2-associated protein and elucidated the molecular mechanisms of the complex formation, IRS-2 ubiquitination and role of this interaction in IGF action in normal and cancer cells in vitro, as well as in zebrash embryos in vivo.

Wiesner et al.24 suggested that the Nedd4 C2 domain binds to its HECT domain and this intramolecular binding inhibits its ubiquitin ligase activity. In this study, we discovered that IRS-2 and the Nedd4 HECT domain competitively bind to the C2 domain. IRS-2 binds to the Nedd4 C2 domain in a region

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Bt2cAMP

IB: Nedd4

Bt2cAMP IGF-I

+ + +

119

196

(kDa)

IB: IRS-2

Pull-down: GST-IRS-2 1-329

119

IB: Nedd4

IB: Nedd4-2

Denaturing

+ +

Bt2cAMP

IRS-2-(Ub)n

200

196

109

pIRS-2

196

IB: IRS-2

IB: Nedd4

IB: pTyr

Nedd4 binding

119

IB: Ub (P4D1)

IB: Ub (FK1)

119

(kDa)

IP: IRS-2(Antibody against IRS-2 C terminus)

IB: IRS-2

Nedd4 binding

(kDa) Pull-down:

GST-IRS-2 1-329

110

110 (kDa)

IB: Nedd4

IP: IRS-2

Membrane

Cytosol

Bt2cAMP

(kDa)

+ +

+ + + +

+ +

IGF-I

IRS-2

(arbitrary unit)

200

IB: IRS-2

1.41.21.00.80.60.40.2

109

IB: Nedd4

IB: IGF-IR

IB: HSP90

0 +

+ +

Bt2cAMP IGF-I

Membrane Cytosol

Bt2cAMP

Control siNedd4 #1

+ + + +

+ +

NS * *

1.2

Bt 2cAMP

0.8

119

Relative intensity

(fold of control)

1.0

IB: Nedd4

0.6

0.4

0.2

IB: IGF-IR

IB: pTyr

pIGF-IR

IGF-I

0 pIGF-IR

pIRS-2

(kDa) IP: IGF-IR

siNedd4 #2

PI3K binding to IRS-2

Control

siNedd4 #1

Bt2cAMP

+ + +

+ + + +

1.2

IGF-I

1.1

1.0

IB: Nedd4

IB: actin

IB: IRS-2

IB: pTyr

IB: p85PI3K

Thymidine incorporation

into DNA (fold of control)

119

0.9

0.8

0.7

0.6

0.5

196

0.4

0.3

0.2

pIRS-2 196

0.1

PI3K binding

Bt2cAMP IGF-I

0 +

+ + + + + +

Control

siNedd4 #1

+ Control

+ siNedd4 #1

(kDa)

IP: IRS-2

Control

siNedd4

Figure 5 | Nedd4 enhances IGF-I signalling in thyrocytes. In the experiments described below, FRTL-5 cells were treated with or without 1 mM dibutyryl cAMP for 24 h. In samples indicated IGF-I, cells were then stimulated with 100 ng ml 1 IGF-I for 1 min (a,d,e) or 24 h (g) after cAMP treatment.

*Signicant difference (Po0.05). (a) Effects of cAMP treatment on the Nedd4 and IRS-2 complex formation. Cell lysates were subjected to immunoprecipitation and immunoblotting as indicated. (b) Effects of cAMP treatment on the Nedd4 and IRS-2 binding in cell-free binding assay. Cell lysates were subjected to pull-down analysis using indicated GST-fused proteins. (c) Effects of cAMP treatment on IRS-2 ubiquitination. Native samples and denatured samples were subjected to immunoprecipitation and immunoblotting as indicated. (d) Effects of cAMP and/or IGF-I treatments on IRS-2 subcellular localization. Cell lysates were fractionated into membrane/cytosol fractions, followed by immunoblotting. Right panel shows the densitometric analysis results. Means.d. of three independent experiments. *Signicant difference (Po0.05, one-way analysis of variance (ANOVA) followed by

TukeyKramer test). (eg) Effect of Nedd4 knockdown on IGF-I signalling and IGF-I-induced DNA synthesis. Cells transfected with Nedd4 siRNAs were treated with dibutyryl cAMP followed by IGF-I stimulation. Lysates were subjected to immunoprecipitation and immunoblotting (e). Densitometric analyses were performed. Tyrosine phosphorylation levels of IGF-IRb and IRS-2 were normalized to their protein levels in immunoprecipitates and p85 PI3K bound to

IRS-2 was normalized to IRS-2 levels in immunoprecipitates. Meanss.d. of seven independent experiments are shown in f. [3H]thymidine incorporation into DNA during 2024 h of IGF-I treatment time was measured. The values were normalized and the meanss.d. of three independent experiments are shown in g. *Signicant difference (Po0.05, one-way ANOVA followed by TukeyKramer test).

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Control siNedd4 #2

IGF-I

119

119 pIGF-IR

IP:IGF-IR

IP: IgG IRS-2

IB: IRS-2

IB: Nedd4

+ +

196

IB: Nedd4

IB: HSP90

IB: IGF-IR

IB: pTyr

Relative intensity

(fold of control)

1.2

Nedd4 binding

DU145 cells

119

Control

siNedd4 #2

siNedd4 #1

PC-3 cells

Nedd4 binding 119

(kDa)

IB: IRS-2

IB: Nedd4

1.00.80.60.40.2

IP: IRS-2

IgG

196

pIGF-IR

pIRS-2

196

PI3K

binding

to IRS-2

PI3K binding

(kDa)

IP:IRS-2

IB: IRS-2

IB: pTyr

IB: p85 PI3K

Control

siIRS-2 #1

siIRS-2 #2

196

(kDa)

Control

siEpsin1 #1

Control

siEpsin1 #2

IB: IRS-2

IB: HSP90

IGF-I

+ +

192

IB: IRS-2

IB: pTyr

pIRS-2

(fold of control)

1.0

0.5

Thymidine incorporation into DNA

(fold of control)

IGF-I

1.2

1.0

0.8

0.6

0.4

0.2

Control

NS NS

pIRS-2

IP: FLAG

IGF-I

0 + +

91 64

IB: Epsin1

IB: HSP70

Control

siEpsin1 #2

siEpsin1 #1

0 + +

(kDa)

siIRS-2 #1

siIRS-2 #2

siNedd4 #1

siNedd4 #2

Figure 6 | Nedd4 enhances IGF-I signalling in prostate cancer cells. (a) The association of Nedd4 with IRS-2. DU145 cells or PC-3 cells were serum starved and cell lysates were subjected to immunoprecipitation and immunoblotting as indicated. (b,c) Effects of knockdown of Nedd4 or Epsin1 on IGF-I signalling in PC-3 cells. Cells transfected with indicated siRNAs were serum starved and then treated with or without 100 ng ml 1 IGF-I for 1 min. Lysates were subjected to immunoprecipitation and immunoblotting as shown. Densitometric analyses of samples with IGF-I stimulation were performed. Tyrosine phosphorylation levels of IGF-IRb and IRS-2 were normalized to their protein levels in immunoprecipitates. p85 PI3K bound to IRS-2 was normalized to IRS-2 levels in immunoprecipitates. Meanss.d. of three to ve independent experiments are shown in the right panel. *Signicant difference from control (Po0.05, oneway analysis of variance (ANOVA) followed by TukeyKramer test). (d) Effects of knockdown of IRS-2 or Nedd4 on IGF-I-induced DNA synthesis in PC-3 cells.

Cells transfected with IRS-2 siRNAs or Nedd4 siRNAs were serum starved for 48 h and then treated with or without 100 ng ml 1 IGF-I for 16 h. [3H]thymidine incorporation into DNA during 1316 h of IGF-I treatment time was measured. The values were normalized and the meanss.d. of three independent experiments are shown. Right insert shows the immunoblotting results. *Signicant difference (Po0.05, one-way ANOVA followed by TukeyKramer test).

overlapping with the HECT-binding site. Thus, we speculate that the dissociation of the C2 and HECT domains not only restores Nedd4 ligase activity but also opens up a substrate-binding site, which enables its recognition of some substrates, including IRS-2. It cannot be ruled out that other factor(s) mediate Nedd4IRS-2 interaction. Crystal structure analysis will aid in understanding the molecular basis of the regulation of this complex formation.

This study demonstrates that Nedd4 induces monoubiquitination at single or multiple Lys residues in the IRS-2 C-terminal region. Ubiquitinated IRS-2 in FRTL-5 cells showed a little higher molecular weight than that in HEK293 cells (Figs 2b and 5c), indicating a different number of monoubiquitinated lysine residues. Nedd4 conjugated more ubiquitin molecules to IRS-2 in a cell-free system than in cells (Fig. 2a). Some ubiquitin molecules may be removed from IRS-2 by de-ubiquitination enzymes in cells. In fact, we observed signicant levels of deubiquitination enzyme USP7 associated with IRS-2 in HEK293 cells35. Further analysis is needed to elucidate how Nedd4 induces monoubiquitination of IRS-2.

Several ubiquitin ligases other than Nedd4 have been shown to ubiquitinate IRS-1/2, but they all induce proteasomal degradation of IRS-1/2 and suppress IGF/insulin signalling3639. In contrast, we demonstrated that Nedd4 enhances IGF-I signalling in this study, indicating a unique role for Nedd4 in IGF signal

regulation. Conjugation of polyubiquitin chains consisting of four or more ubiquitin molecules to substrate proteins is required for their proteasomal degradation40, which can explain why Nedd4 does not induce IRS-2 degradation. Our data suggest that Nedd4 increases the association of IRS-2 with Epsin1 and this in turn promotes the translocation of IRS-2 from the cytosol to the plasma membrane. Epsin1 UIMs mediate the association of ubiquitinated IRS-2 with Epsin1. Epsin1 is localized to the plasma membrane and facilitates the formation of clathrin-coated invaginations through its other domains and motifs (schematically represented in Fig. 4b)30,41. We speculate that Epsin1 may recruit the ubiquitinated IRS-2 to the position adjacent to the plasma membrane through physical interaction. As IGF-IR internalizes through the clathrin-dependent pathway42, it is attractive to postulate that Nedd4 may induce the co-localization of IRS-2 and IGF-IR at a specic plasma membrane compartment, where high levels of Epsin1 are present and clathrin-coated pits are forming. Their co-localization facilitates phosphorylation of IRS-2 by the IGF-IR tyrosine kinase. This model is strongly supported by our result that Epsin1 knockdown suppressed the effect of Nedd4 on IRS-2 tyrosine phosphorylation (Fig. 4c). It is unclear why different IGF-I-dependency of IRS-2 translocation was shown in subcellular fractionation of HEK293 cells and FRTL-5 cells (Figs 4d and 5d).

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* *

2.102.052.001.951.901.851.801.751.701.651.601.551.50

Control MO Irs2 MO

Venus Nedd4-Venus 2.15 30

29 28 27 26 25 24 23 22 21 20 19 18

Body length (mm)

Irs2 MO

Somite number

Irs2 MO

Venus Nedd4-

Venus

Venus Nedd4-Venus

IGFs IGF-IR

Monoubiquitination

Nedd4IRS-2 IRS-2

IRS-2

Epsin1 Tyrosine phosphorylation

IGF mitogenic activity

pY pY

Nedd4-IRS-2 complex formation by cAMP stimulus in thyroid cells

Recruitment to plasma membrane

Nedd4 overexpression in prostate cancer cells

Figure 7 | Nedd4 accelerates zebrash embryonic growth through Irs-2. (a) Nedd4-Venus mRNA or Venus mRNA was co-injected with eitherIrs2 MO or control MO into one- to two-cell stage zebrash embryos. The embryos were raised to 22 h post fertilization and representative embryos are shown. Scale bar, 500 mm. (b,c) Values of body length and somite number are plotted. Blue lines indicate the meanss.e.m. (n 2134). *Signicant

difference from the control group (Po0.05, one-way analysis of variance followed by TukeyKramer test). (d) Working model on how Nedd4 enhances IGF signalling and mitogenic activity (a detailed explanation is given in the Discussion).

Further studies are needed to elucidate molecular mechanisms underlying Epsin1-mediated modulation of IGF-I signalling.

Thyroid epithelial cells synergistically proliferate in response to IGF-I and TSH in vitro4345, which is thought to be important for thyroid morphogenesis and thyroid hormone homeostasis46. We previously reported that TSH or other cAMP-generating reagents enhance IGF-I-induced cell proliferation in thyroid FRTL-5 cells44. In this study, we showed that cAMP stimulus induces the association of Nedd4 with IRS-2, IRS-2 monoubiquitination and recruitment of IRS-2 to the membrane fraction. Nedd4 is required for the cAMP-dependent enhancement of IRS-2 tyrosine phosphorylation and DNA synthesis induced by IGF-I. We conclude that cAMP stimulus triggers the mechanisms by which Nedd4 enhances IRS-2-mediated signalling. In various endocrine tissues, IGFs and tropic hormones synergistically regulate cell proliferation, differentiation and hormone synthesis6. As tropic hormones generally use the cAMP pathway, we speculate that Nedd4 may play roles in the cross-talk between IGFs and other hormones.

In this study, we also detected the Nedd4IRS-2 complex in prostate cancer cells. Nedd4 knockdown suppressed IGF-I-induced IRS-2 tyrosine phosphorylation and DNA synthesis, suggesting that Nedd4 enhances IRS-2-mediated signalling and IGF-I mitogenic activity. It has been reported that Nedd4 promotes prostate cancer development through the ubiquitination and degradation of phosphatase and tensin homologue (PTEN)17. However, PC-3 cells are decient in PTEN47, excluding the possibility that the effects of Nedd4 are mediated by the regulation of PTEN in our experiments. The Nedd4-IRS-2 binding may represent a novel drug target at least in prostate cancer. Accumulating evidence suggests that Nedd4 is overexpressed in various cancers and functions as a

tumour-promoting factor1721. It is important to investigate the contribution of the Nedd4-IRS-2 complex to the development of various cancers.

The results of this study show that Nedd4 accelerates zebrash embryonic growth through Irs2. In mice, Nedd4 deciency causes delayed embryonic growth16. In both cases, Nedd4 modulates embryonic growth without major developmental abnormality of any specic organs. Given that IGF signalling deciency in various species causes growth retardation without patterning abnormalities24,33, these results imply that Nedd4 is an evolutionarily conserved factor that plays a positive role in embryonic growth, possibly through modulating IGF-IR-IRS-2 signalling activities.

It has been reported that Nedd4 acts on multiple IGF signal transducers. As described above, in mouse embryonic broblasts, Nedd4 is required to maintain the cell-surface IGF-IR levels16. In contrast, others reported that Nedd4 induces degradation of IGF-IR15,48,49. A recent report suggested that Nedd4 may enhance IGF-I-induced IRS-1 tyrosine phosphorylation by antagonizing PTEN, which functions as a tyrosine phosphatase for IRS-1 (ref. 50). Nedd4 has also been reported to regulate the stability and subcellular localization of Akt and PTEN17,51,52. In our study, Nedd4 affected neither total nor cell-surface IGF-IR levels, and had little effect on the signalling downstream of IRS-1. These discrepancies may be due to different experimental conditions or cellular models used. It is also possible that these multiple sites of Nedd4 action allow the ne-tuning of IGF signalling in a context-dependent manner.

In conclusion, our study demonstrated that Nedd4 enhances IGF-I signalling and mitogenic activity through a novel mechanism (Fig. 7d). Nedd4 associates with IRS-2 and conjugates monoubiquitin to IRS-2. Ubiquitinated IRS-2 is in turn

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NATURE COMMUNICATIONS | DOI: 10.1038/ncomms7780 ARTICLE

recognized by Epsin1, which possibly recruits IRS-2 to the plasma membrane. Consequently, Nedd4 enhances IGF-I-induced tyrosine phosphorylation of IRS-2 by the IGF-IR kinase, which leads to the augmentation of IGF-I signalling and mitogenic activity.

Methods

Materials. Recombinant human IGF-I was kindly donated by Dr Toshiaki Ohkuma (Fujisawa Pharmaceutical Co., Osaka, current Astellas Pharma Inc., Tokyo, Japan).

Several anti-IRS-2 antibodies were used in this study. One was obtained from Santa Cruz Biotechnology (sc-8299, Santa Cruz, CA, USA) and another was a home-made antibody against the IRS-2 C-terminal peptide12 (used in Fig. 5a and Supplementary Fig. 1a,c). The other anti-IRS-2 antibody against aa 9761094 (used in Supplementary Fig. 6a, 06-506) was purchased from Millipore (Billerica, MA, USA). Anti-Nedd4 antibody (07-049), anti-p85 PI3K antibody (06-195) and anti-HRS antibody (ABC35) were obtained from Millipore. Anti-myc antibody (clone 9E10, sc-40), anti-ubiquitin antibody (clone P4D1, sc-8017), anti-IGF-IR antibody (sc-713), anti-Grb10 antibody (sc-1026), anti-Eps15 antibody (sc-534), anti-Epsin1 antibody (sc-25521), anti-HSP90 antibody (sc-13119) and horseradish peroxidase (HRP)-linked anti-mouse IgM antibody (sc-2064) were obtained from Santa Cruz Biotechnology. Anti-Eps15R antibody (EP1146Y), anti-TSG101 antibody (EPR7131B) and anti-HSP70 antibody (EP1007Y) were obtained from Abcam (Cambridge, UK). Anti-Nedd4-2 antibody (4013) and anti-ubiquitin remnant motif antibody-conjugated beads were purchased from Cell Signaling (Danvers, MA, USA), and anti-ubiquitin antibody (clone FK1) was from Nippon Bio-Test Laboratories (Tokyo, Japan). Anti-phosphotyrosine antibody (clone PY20), anti-FLAG antibody (clone M2), anti-FLAG M2 antibody-conjugated agarose beads and rabbit IgG were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-HA antibody (clone 3F10) was from Roche (Basel, Schweiz). HRP-linked anti-mouse IgG antibody (NA931) and HRP-linked anti-rabbit IgG antibody (NA934) were purchased from GE Healthcare (Buckinghamshire, UK).

Smal interfering RNAs (siRNAs; 21-mer, having overhanging dTdT at the 30 terminus) were designed as follows: human IRS-2 #1, 50-UCGGCUUCGUGAAGC UCAAdTdT-30, #2, 50-GGCUGAGCCUCAUGGAGCAdTdT-30; human Nedd4 #1, 50-GCAAAUGGCUGCUUUUAAAdTdT-30, #2, 50-CCAUGAAUCUAGAAG AACAdTdT-30; rat Nedd4 #1, 50-CCAUGAGUCUAGAACAACAdTdT-30, #2, 50-GAAGCAGACUGACAUUCCAdTdT-30; rat Nedd4-2, 50-GAUCACUUAUCC UAUUUCAdTdT-30; human Epsin1 #1, 50-CCAGCUCCGUCUCAGUCCUd TdT-30; #2, 50-GCUCCCUCAUGUCAGAGAUdTdT-30. These siRNAs and a universal negative control RNA were synthesized by Nippon EGT (Toyama, Japan).

Rat IRS-1 complementary DNA and human IRS-2 cDNA were prepared as described elsewhere53. Mouse Nedd4 cDNA was kindly donated by Dr Sharad Kumar (Queensland University of Technology, Brisbane, Australia.). Mouse Nedd4-2 cDNA was amplied by PCR from mouse brain cDNA library. Ubiquitin cDNA was kindly provided by Dr Takeshi Imamura (Japanese Foundation for Cancer Research, Tokyo, Japan). Rat Epsin1 cDNA and human USP15 cDNA were amplied by PCR from H4IIE hepatocyte cDNA library and Huh7 hepatocyte cDNA library, respectively. Partial zebrash Irs2 cDNA (NM_200315) was amplied by PCR from zebrash embryo cDNA library. It contains 50-untranslated region (UTR) and translation start site of Irs2. cDNAs with deletion or site-directed mutation were generated by standard restriction enzyme- or PCR-based methods.

For the construction of expression plasmids, these cDNAs were subcloned into the following vectors; pGEX (GE Healthcare) for the expression of N-terminal glutathione (GST)-tagged protein; modied pEGFP-N1 (Clontech, Mountain View, CA, USA) in which green uorescent protein gene between the BamHI and NotI sites had been removed, for the expression of intact protein; pFLAG-CMV2 (Sigma-Aldrich) for N-terminal FLAG-tagged protein; pmyc-CMV5 (gift from Dr Jun Nakae, Keio University, Tokyo, Japan) for N-terminal myc-tagged protein; pmyc-CMV5-FLAG that we had generated by inserting FLAG peptide sequence into SalI site, for protein with N-terminal myc-tag and C-terminal FLAG tag; pRKHA for N-terminal HA-tagged protein; pCS2-Venus that was used as template for capped messenger RNA synthesis. Using these vectors, we made Escherichia coli expression plasmids for GST-IRS-2 (aa 1329) and GST-Nedd4 (wild type;aa 1226; 1551; 227551; 227887; 552887; deletion of 227551 (D227551); D251280, 407436 and 462491 (DWW)). We also made mammalian expression plasmids for intact IRS-2, FLAG-IRS-2 (wild type; aa 1545; 1329; 1134; 137329; 1371338; 3301338), FLAG-IRS-2-Ub (in which C-terminal di-Gly of ubiquitin was deleted (DGG) to prevent its cross-linking to other proteins by ubiquitin ligases), FLAG-IRS-2-UbI44A (ubiquitin is DGG-type), myc-IRS-2-FLAG (wild type and a series of mutants in which indicated Lys residues were substituted to Arg), myc-IRS-2 (wild type, C1-C3 KR and K1331R), myc-IRS-2-Ub (ubiquitin is DGG-type), myc-IRS-2-UbI44A (ubiquitin is DGG-type), myc-Nedd4 (wild type;

aa 1551, 503887 C854S; 552887 E541A, C854S; I95A, L96A; S126A, L127A; E541A; Y592A; F694A; C854S), FLAG-Nedd4 (wild type; aa 1502; 1502 I95A, L96A; 1502 S126A, L127A), FLAG-Nedd4-2, HA-ubiquitin (HA-Ub), HA-UbK0 (in which all Lys residues were substituted to Arg for prevention of polyubiquitin chain formation), Epsin1-FLAG (wild type; D136-256 (DUIM)), FLAG-IRS-1 and

myc-IRS-1-FLAG. IGF-IR-FLAG plasmid was kindly provided by Dr Iwaki (Osaka University, Osaka, Japan). For the protein expression in zebrash embryo, Nedd4-Venus plasmids and 50-UTRIrs2-Venus plasmids were generated as template for capped RNA synthesis. RNA sequence of 50-UTRIrs2-Venus plasmids is shown in Supplementary Fig. 8a.

The Irs2 MO (50-CCATACTTGCGTTGACAGTTTATTT-30) targeting the translational start site of zebrash Irs2 mRNA (NM_200315) and the control MO (50-CCTCTTACCTCAGTTACAATTTATA-30) were synthesized by Gene Tools,

LLC (Philomath, OR, USA).Other chemicals were of the reagent grade available commercially.

Cell culture and transfection. HEK293 cells (kind gifts from Dr Koichi Suzuki (National Institute of Infectious Diseases, Tokyo, Japan)), HEK293T cells, prostate cancer DU145 cells and PC-3 cells (kind gifts from Dr Akio Matsubara (Hiroshima University)) were maintained in DMEM medium (Nissui Pharmaceutical Co., Tokyo, Japan) supplemented with 10% fetal bovine serum (Biomol, Nuaille, France) and antibiotics. Rat thyroid FRTL-5 cells were kindly provided by the late Dr Leonard Kohn (Ohio University and Edison Biotechnology Institute, OH, USA) and the Interthyr Research Foundation (Baltimore, MD, USA). FRTL-5 cells were cultured in Coons F-12 medium (Sigma-Aldrich) supplemented with0.33 mg ml 1 glutamine, MEM non-essential amino acids (ICN Biochemicals, Costa Mesa, CA, USA), 5% newborn bovine serum, 1 mU ml 1 TSH, 10 mg ml 1 insulin, 5 mg ml 1 transferrin and antibiotics. For FRTL-5 cells to become quiescent, the cells were washed twice with Hanks balanced salt solution (Nissui) and cultures were continued for an additional 2448 h in starvation medium (Coons F-12 medium supplemented with 0.33 mg ml 1 glutamine, MEM non-essential amino acids and 0.1% bovine serum albumin). The quiescent FRTL-5 cells were then cultured in starvation medium supplemented with or without 1 mM dibutyryl cAMP for 24 h. In some experiments, the cells were washed three times with Hanks balanced salt solution and then cultured in starvation medium for 30 min, followed by IGF-I stimulation for indicated durations.

HEK293 cells were transfected with plasmids and/or siRNA using Lipofectamine 2000 (Life Technologies, Gaithersburg, MD, USA). The maximum transfection efciency was about 90%, based on the evaluation using cells transfected with plasmids encoding green uorescent protein. HEK293T cells were transfected with plasmids using polyethylenimine (Linear, molecular weight 25,000 Da; Polysciences, Warrington, PA, USA) as follows. Subconuent cells on 60 mm dishes (2 ml of 10% FBS-DMEM) were prepared. Two micrograms of plasmid was mixed with 6 ml of polyethylenimine solution (1 mg ml 1) in 200 ml

Opti-MEM (Life Technologies). After incubation for 15 min, the mixture was added to cells. Twelve hours later, the culture medium was changed to fresh growth medium and cell culture was continued for 12 h. Cells were then serum starved for 18 h, followed by indicated biochemical experiments. FRTL-5 cells and PC-3 cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies).

MS analysis of IRS-2-associated proteins. Immunoprecipitates with antibodies against IRS-2 C terminus were subjected to SDSPAGE, followed by gel staining using SilverQuest (Life Technologies). Gel slices were destained according to the protocols attached to the gel staining kits and then equilibrated with 50 mM NH4HCO3 for 15 min. After the reagent was removed, gel slices were mixed with dehydration buffer (50 mM NH4HCO3, 50% CH3CN) and vortexed for 15 min. After the dehydration procedure was repeated again, gels were dried by vacuum evaporator centrifuge VEC-260 (ASAHI GLASS, Chiba, Japan). Gels were then reswollen in reducing buffer (10 mM dithiothreitol, 25 mM NH4HCO3) and incubated for 1 h at 56 C. After the equal volume of alkylation buffer (55 mM iodoacetamide, 25 mM NH4HCO3) was added, the mixtures were shaded and vortexed for 30 min at room temperature. Gels were then equilibrated with 50 mM NH4HCO3, dehydrated with 50% CH3CN and dried by VEC-260 as described above. After reswelling on ice in digestion buffer (10 mg ml 1 Trypsin Gold, Mass

Spectrometry Grade (Promega, Madison, WI, USA), 50 mM NH4HCO3, 5 mM CaCl2), gels were incubated for 16 h at 37 C. To extract the tryptic peptides, 5% triuoroacetic acid (TFA) was added and the supernatant was recovered. Gels were then vortexed in 5% TFA/30% CH3CN for 5 min and sonicated for 20 min, and the supernatant was recovered. The same procedure was repeated using 5% TFA/50% CH3CN and 5% TFA/70% CH3CN sequentially. All supernatants were collected and concentrated to 10 ml by VEC-260 and TFA (nal concentration, 0.1%) was added to the samples. To desalt the sample, ZipTip mC18 (Millipore) was swollen in 0.1% TFA/50% CH3CN and equilibrated with 0.1% TFA/2% CH3CN, and the samples were then adsorbed and washed with 0.1% TFA/2% CH3CN. The tryptic peptides were eluted with 1 ml of 0.1% TFA/66% CH3CN onto a 100-well gold sample plate (Applied Biosystems, Framingham, MA, USA), mixed with 1 ml of matrix solution (10 mg ml 1 a-cyano-4-hydroxy cinnamic acid in 0.2% TFA/60%

CH3CN) and dried at room temperature. The samples were analysed by matrix-assisted laser desorption/ionizationtime-of-ight MS Voyager-DE STR (Applied Biosystems) in peptide-sensitivity reector mode. The spectra were obtained by the accumulation of 2001,000 consecutive laser shots and calibrated by mass values of auto-digested trypsin peptides. The mass data were subjected to MASCOT PMF search tools (Matrix Science, London, UK).

Pull-down assay. Cells were lysed in ice-cold lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM NaF, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 500 mM

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Na3VO4, 10 mg ml 1 p-nitrophenyl phosphate, protease inhibitor cocktail (Sigma-Aldrich, P8340)). In pull-down assay, lysates were incubated with 100 pmol of

GST-fused proteins for 2 h and then 10 ml of GSTSepharose beads (GE Health-care). After 1 h incubation, beads were washed four times with ice-cold lysis buffer and proteins were subjected to SDSPAGE followed by immunoblotting.

Immunoprecipitation and immunoblotting. Cells were lysed with ice-cold lysis buffer. The lysates were centrifuged at 15,000g for 10 min at 4 C. The protein assay of the supernatant was carried out using a protein assay kit (Bio-Rad, Hercules, CA, USA). Equal amounts of proteins of each sample were mixed with 1/4 volumeof 4 Laemmlis buffer (0.4 M Tris-HCl pH 6.8, 8% SDS, 20% glycerol, 10%

2-mercaptoethanol, 0.2% bromophenol blue). The mixtures were boiled for 5 min and subjected to SDSPAGE. In immunoprecipitation, equal amounts of proteins of each sample were mixed with indicated antibodies (their concentrations were as recommended by manufacturers). Samples were incubated at 4 C for 12 h and then mixed with 10 ml of Protein A-Sepharose or Protein G-Sepharose (GE Healthcare). After the additional incubation for 1 h, precipitates were washed with ice-cold lysis buffer three times and diluted with 1 Laemmlis buffer. After boiling

for 5 min, the supernatant was subjected to SDSPAGE. The immunoprecipitation using anti-FLAG antibody-conjugated agarose beads followed by elution with FLAG peptide (Sigma-Aldrich) was performed according to the manufacturers recommended protocols. The eluates were mixed with 1/4 volume of 4 Laemmlis

buffer, boiled for 5 min and subjected to SDSPAGE. After SDSPAGE, proteins were transferred onto a polyvinylidene diuoride membrane. The indicated rst antibodies and HRP-conjugated second antibodies were hybridized according to standard immunoblotting protocols (their concentrations were as recommended by manufacturers). Chemiluminescence reactions were carried out using SuperSignal West Pico/Femto (Thermo Fisher Scientic, Rockford, IL, USA) and the luminescence was exposed onto X-ray lm (X-Omat, Kodak, Tokyo, Japan). Densito-metric analysis was carried out using ImageJ 1.43u programme (National Institutes of Health, Bethesda, MD, USA). Uncropped scans of blots are shown in Supplementary Figs 913.

In sample preparation for the detection of ubiquitination signals as well as the interaction of IRS-2-Epsin1 (or other ubiquitin-binding proteins), cells were lysed with lysis buffer supplemented with 2 mM N-ethylmaleimide, to inhibit de-ubiquitinating enzymes. In addition, protein samples for the detection of ubiquitination signals were denatured by mixing with 2% SDS and boiling for5 min, to disassemble protein complex, and then diluted to 20 times by lysis buffer followed by immunoprecipitation. After SDSPAGE and transfer to a polyvinylidene diuoride membrane, the membrane was denatured by incubating in denaturing buffer (6 M guanidine-HCl, 20 mM Tris-HCl pH7.5, 5 mM b-mercaptoethanol, 1 mM phenylmethyl sulphonyl uoride) for 30 min and then washed several times with 0.1% Tween20/PBS before immunoblotting using anti-ubiquitin antibody.

Cell-free ubiquitination. HEK293T cells overexpressing FLAG or FLAG-IRS-2 (one 100-mm culture dish of cells per assay) were lysed and subjected to immunoprecipitation using 20 ml anti-FLAG antibody-conjugated beads (Sigma-

Aldrich). Immunoprecipitates on beads were mixed with 150 ng His-human E1 (Biomol, Plymouth, PA, USA), 300 ng His-human UbcH5b (Biomol), 750 ng GST-Nedd4 or GST produced by us, 7.5 mg ubiquitin (Sigma-Aldrich) and 2 mM

ATP, in the reaction buffer (25 mM Tris-HCl pH7.5, 120 mM NaCl, 0.5 mM dithiothreitol and 2 mM MgCl2). After the incubation for 2 h at 30 C, immunoprecipitates were washed with lysis buffer four times, followed by immunoblotting.

LC-MS/MS analysis to determine IRS-2 ubiquitination sites. FLAG-tagged IRS-2 was immunoprecipitated with anti-FLAG antibody-conjugated beads. The immunoprecipitates were mixed with 100 ml of trypsin solution (10 ng ml 1

Trypsin Gold Mass Spectrometry Grade, 50 mM ammonium bicarbonate, 0.01% RapiGest SF (Waters, Milford, MA, USA)), trypsin and Asp-N solution (10 ng ml 1

Trypsin Gold, 6.6 ng ml 1 Asp-N (Promega), 50 mM ammonium bicarbonate,0.01% RapiGest SF), or trypsin and chymotrypsin solution (10 ng ml 1 Trypsin Gold, 10 ng ml 1 chymotrypsin (Promega), 100 mM Tris-HCl pH8.0, 10 mM

CaCl2, 0.01% RapiGest SF). The samples were incubated at 37 C for 12 h. After a brief centrifugation, supernatants were recovered, mixed with 100 ml of TFA and then desalted using GL-Tip SDB and GL-Tip GC (GL Sciences, Tokyo, Japan). The samples were diluted with IAP buffer (Cell Signaling), mixed with anti-ubiquitin remnant motif antibody-conjugated beads and then incubated at 4 C for 2 h. The immunoprecipitates were washed with IAP buffer twice and with water three times. Peptides were eluted by 0.15% TFA, desalted with GL-Tip SDB and GL-Tip GC, and then subjected to LC-MS/MS analysis (nano-liquid chromatography column (EASY-nLC 1000, Thermo Fisher Scientic) coupled to Q Exactive mass spectrometer (Thermo Fisher Scientic))54. Data were analysed by Proteome Discoverer software (Thermo Fisher Scientic) and ProteinPilot software (AB SCIEX, Framingham, MA). Peptide quantication was carried out by parallel reaction monitoring, a MS/MS-based quantication method. The extracted peptides were analysed by Q Exactive in targeted MS/MS mode. Raw les were processed by PinPoint software (Thermo Fisher Scientic). Ion chromatograms were extracted

with a mass tolerance of 5 p.p.m. The area under the curve of selected fragment ion was calculated. The AUCs of each individual fragment ions were then summed to obtain AUCs at the peptide level54.

Biotinylation of cell surface proteins. Cells were incubated with 1 mg ml 1 NHS-LC biotin (Thermo Fisher Scientic)/PBS for 20 min at 4 C and then washed twice with 100 mM glycine/PBS. The cells were then lysed with lysis buffer, followed by immunoprecipitation and blotting with HRP-conjugated streptavidin (Sigma-Aldrich) and indicated antibodies.

Subcellular fractionation. Cells were homogenized in ice-cold detergent-free buffer (40 mM Tris-HCl pH 7.4, 120 mM NaCl, 1 mM EDTA, 1 mM EGTA, 10 mM pyrophosphate, 50 mM NaF, 500 mM Na3VO4, 10 mg ml 1 p-nitrophenyl phosphate, protease inhibitor cocktail). Samples were then subjected to ultra-centrifugation (100,000g, 1 h). The supernatants were recovered as cytosol fractions. The precipitates were solubilized with lysis buffer as described above (containing 1% Triton X-100), followed by centrifugation (15,000g, 10 min) and the supernatants were recovered as membrane fractions.

Thymidine incorporation into DNA. Cells were cultured in 48-well plates or 24-well plates and [Methyl-3H]thymidine (0.3 mCi per well; 1 mCi ml 1, GE

Healthcare) was added to each well for indicated durations. The labelling was stopped by adding 500 ml of 1 M ascorbic acid. The cells were washed twice with ice-cold PBS and twice with ice-cold 10% trichloroacetic acid. Trichloroacetic acid -precipitated materials were solubilized with 250 ml of 0.2 N NaOH/0.1% SDS and mixed into 5 ml clear-sol II (Nakalai tesque). The radioactivity was measured by liquid scintillation counter (Aloka, Tokyo, Japan).

Zebrash experiments. Wild-type zebrash (Danio rerio) were kept at 28 C under a light/dark cycle of 14 and 10 h, and fed twice daily. The wild-type embryos obtained by natural breeding were raised at 28.5 C and staged55. Capped RNAs were synthesized using mMESSAGE mMACHINE Kit (Life Technologies). Venus mRNA (200 pg per embryo), Nedd4-Venus mRNA (1,200 pg per embryo) or 50-UTRIrs2-Venus RNA (250 pg per embryo) was co-injected with either control

MO or Irs2 MO (4 ng per embryo) into embryos at one- to two-cell stage. Embryos were then placed in embryo-rearing medium34 and kept at 28.5 C. Body length and somite number were measured at 22 h post fertilization. Body length was dened as the curvilinear distance from the middle of the otic vesicle to the end of tail through the trunk midline. The expression of Nedd4-Venus was conrmed by uorescence and immunoblotting. Specic translation block with Irs2 MO were conrmed by the decreases of 50-UTRIrs2-Venus uorescence. All experiments were conducted in accordance with the guidelines approved by the Ethical Committee of Experimental Animal Care at Ocean University of China.

Statistical analysis. The results shown are the means.d., except for Fig. 7b,c. In Fig. 7b,c, the results are shown as the means.e.m. Data were analysed by Students t-test or one-way factorial analysis of variance followed by Tukey Kramer post-hoc multiple comparison test. Po0.05 was considered statistically signicant (shown as * in graphs).

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Acknowledgements

We thank Dr Leonard Girnita (Karolinska Hospital, Stockholm, Sweden), Dr Ignacio Torres-Aleman (Cajal Institute, Madrid, Spain), Dr Yusuke Higashi (Tulane University, New Orleans, LA, USA), Dr Robert J. Smith (Brown University, Providence, RI, USA) and Dr Yoshihiro Tsuchiya (Hiroshima University) for helpful discussions. We thank Dr Ohkuma, Dr Kumar, Dr Imamura, Dr Nakae, Dr Iwaki, Dr Suzuki and Dr Matsubara for kind donations of IGF-I, cDNAs, plasmids and cells. We also acknowledge the help of Dr Susan Hall (The University of North Carolina at Chapel Hill) and Mr John Allard in proof-reading the manuscript. A part of this work was carried out at the Analysis Center of Life Science, Natural Science Center for Basic Research and Development, Hiroshima University. This work was partially supported by the following grants: Grant-in-Aid for Young Scientists (B)#22780249 and (B)#24790928 from the Japan Society for the Promotion of Science (JSPS), A-STEP Feasibility Study Stage #AS232Z02241G from Japan Science and Technology Agency and Grant from Tsuchiya Memorial Medical Foundation to TF; Grant-in-Aid for JSPS Fellows #13J07852 to H.Y.; Grant-in-Aid for Scientic Research on Innovative Areas #24112008 from JSPS to Y.S.; Grant-in-Aid for Specially Promoted Research #21000012 from JSPS to K.T.; Grant-in-Aid for Scientic Research (B)#23390242 from JSPS to T.A.; Grant-in-Aid for Scientic Research(A)(2)#16208028, (A)#22248030 and (S)#25221204; Core-to-core programme from JSPS and Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry to S.-I.T.

Authors contributions

T.F., H.Y., H.F., H. Kamei (H.K.1), F.H. and S.-I.T. conceived and designed these experiments. T.F., H.Y., H.F., H.K.1, F.H., J.L., C.D., Y.S., K.T., S.I. and T.N. performed the experiments. T.F., H.Y., H.F., H.K.1, F.H., J.L., C.D., Y.S., K.T., S.I., T.N., K.C., Y.N.,H.Kamata (H.K.2), T.A. and S.-I.T. analysed the data and contributed reagents/materials/ analysis tools. T.F., H.Y., H.F., H.K.1, F.H., C.D. and S.-I.T. wrote the paper. S.-I.T. took the primary responsibility for nal content. T.F., H.Y., H.F., H.K.1, F.H., C.D., Y.S., K.T., K.C., Y.N., H.K.2, T.A. and S.-I.T. read and approved the nal manuscript.

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How to cite this article: Fukushima, T. et al. Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity. Nat. Commun. 6:6780doi: 10.1038/ncomms7780 (2015).

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Insulin-like growth factors (IGFs) induce proliferation of various cell types and play important roles in somatic growth and cancer development. Phosphorylation of insulin receptor substrate (IRS)-1/2 by IGF-I receptor tyrosine kinase is essential for IGF action. Here we identify Nedd4 as an IRS-2 ubiquitin ligase. Nedd4 monoubiquitinates IRS-2, which promotes its association with Epsin1, a ubiquitin-binding protein. Nedd4 recruits IRS-2 to the membrane, probably through promoting Epsin1 binding, and enhances IGF-I receptor-induced IRS-2 tyrosine phosphorylation. In thyroid FRTL-5 cells, activation of the cyclic AMP pathway increases the association of Nedd4 with IRS-2, thereby enhancing IRS-2-mediated signalling and cell proliferation induced by IGF-I. The Nedd4 and IRS-2 association is also required for maximal activation of IGF-I signalling and cell proliferation in prostate cancer PC-3 cells. Nedd4 overexpression accelerates zebrafish embryonic growth through IRS-2 in vivo. We conclude that Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity.

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Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity

Author

Fukushima, Toshiaki; Yoshihara, Hidehito; Furuta, Haruka; Kamei, Hiroyasu; Hakuno, Fumihiko; Luan, Jing; Duan, Cunming; Saeki, Yasushi; Tanaka, Keiji; Iemura, Shun-ichiro; Natsume, Tohru; Chida, Kazuhiro; Nakatsu, Yusuke; Kamata, Hideaki; Asano, Tomoichiro; Takahashi, Shin-ichiro

Pages

6780

Publication year

2015

Publication date

Apr 2015

Publisher

Nature Publishing Group

e-ISSN

20411723

Source type

Scholarly Journal

Language of publication

English

DOI

https://doi.org/10.1038/ncomms7780

ProQuest document ID

1673371151

Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity

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