The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans

Abstract

Aging is the major risk factor for many human diseases and degeneration. Thus, clinically effective medicine could delay the process of aging and aging-related diseases are desperately wanted. In traditional Chinese medicine (TCM), some were claimed to slow down aging. Qingyangshen (Cynanchum otophyllum schneid) is such a TCM. Here, we assayed the longevity effect of compound Otophylloside B (Ot B), a C-21 steroidal glycoside isolated from Qingyangshen, in Caenorhabditis elegans, which is a popular model for aging research. Our results showed that Ot B could modestly extend the lifespan of C. elegans, delay the age-related decline of body movement and improve the stress resistance. Further investigating the molecular mechanism of lifespan extension effect revealed that Ot B could activate the FOXO transcription factor DAF-16. Ot B could not further extend the lifespan of long-lived mutant of insulin/IGF-1-like receptor (daf-2). In addition, Ot B also requires SIR-2.1 and CLK-1 which is an enzyme in ubiquinone synthesis, for lifespan extension.

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Jie Yang . Qin-Li Wan . Quan-Zhang Mu . Chun-Feng Wu . Ai-Jun Ding . Zhong-Lin Yang . Ming-Hua Qiu . Huai-Rong Luo

Received: 28 April 2015 / Accepted: 25 May 2015 / Published online: 26 June 2015 The Author(s) 2015. This article is published with open access at Springerlink.com

Abstract Aging is the major risk factor for many human diseases and degeneration. Thus, clinically effective medicine could delay the process of aging and aging-related diseases are desperately wanted. In traditional Chinese medicine (TCM), some were claimed to slow down aging. Qingyangshen (Cynanchum otophyllum schneid) is such a TCM. Here, we assayed the longevity effect of compound Otophylloside B (Ot B), a C-21 steroidal glycoside isolated from Qingyangshen, in Caenorhabditis elegans, which is a popular model for aging research. Our results showed that Ot B could modestly extend the lifespan of C. elegans, delay the age-related decline of body movement and improve the stress resistance. Further investigating the molecular mechanism of lifespan extension effect revealed that Ot B could activate the FOXO transcription factor DAF-16. Ot B could not further extend the lifespan of long-lived mutant of insulin/IGF-1-like receptor (daf-2). In addition, Ot B also requires SIR-2.1 and CLK-1 which is an enzyme in ubiquinone synthesis, for lifespan extension.

Keywords Otophylloside B Caenorhabditis elegans Aging DAF-16/FOXO IIS signaling pathway

Jie Yang and Qin-Li Wan have contributed equally to this work.

Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s13659-015-0064-4

Web End =10.1007/s13659-015-0064-4 ) contains supplementary material, which is available to authorized users.

J. Yang Q.-L. Wan Q.-Z. Mu C.-F. Wu A.-J. Ding

Z.-L. Yang M.-H. Qiu H.-R. Luo (&)

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 134 Lanhei Road, Kunming 650201, Yunnan, China e-mail: [email protected]

J. Yang Q.-L. Wan A.-J. Ding Z.-L. Yang

University of Chinese Academy of Sciences, Beijing 100049, China

J. YangThe Second Afliated Hospital of Kunming Medical University, Kunming 650101, China

1 Introduction

The identication of chemical interventions that can ameliorate age-related illness and degeneration has been an important aspect of current aging research. Pharmacological compounds that could slow down the normal aging process and extend the lifespan, could also delay the progression of age-related disorders, such as Alzheimers disease and cardiovascular diseases. In addition, the characterizing the mechanism of the lifespan extension effect of drugs can help to understand endogenous mechanisms involved in longevity. The genetic pathways identied to regulate longevity were turned out to be evolutionarily conserved. For example, the insulin/IGF-1-like signaling (IIS) [13] is highly conserved to inuence longevity in model organisms ranging from worms to mice [4].

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The free-living soil nematode C. elegans has been a leading system for studying genetic and pharmacologic inuences on lifespan, mainly because of its short lifespan and amenability to genetic manipulation. In addition, a number of compounds have been reported to extend C. elegans lifespan, such as a variety of antioxidant compounds [5, 6], complex mixtures derived from plants [7, 8], a sirtuin activator resveratrol [9, 10], an antihyperglycemic drug metformin [11, 12], TOR inhibition rapamycin [13], nonsteroidal anti-inammatory drugs (NSAIDs) (e.g. aspirin), as well as anti-convulsant medicines (e.g. ethosuximide) [14, 15].

Otophylloside B (Ot B), a C-21 steroidal glycoside, was isolated from C. otophyllum (Chinese name Qingyangshen) [16], which was a folk medicinal plant endemic to Yunnan province of China, whose root was used for the treatment of epilepsy, rheumatic pain, kidney weakness, and muscle injuries by the local people in its growing area. However, the molecular mechanism activated by Ot B remains vague. In our study, we found that Ot B could lengthen the lifespan of C. elegans, delay the age-related decline of body movement and improve heat resistance. We further demonstrated that Ot B-mediated lifespan extension depend on the FOXO transcription factor DAF-16. The effect of Ot B on lifespan could share similar phenotypic features as IIS signaling pathway because it failed to further extend the lifespan of long-live insulin-like receptor mutant daf-2 [17]. In addition, Ot B extending the lifespan also requires SIR-2.1 and CLK-1.

2 Results and Discussion

2.1 Ot B Extended the Lifespan of C. elegans, Delayed Age-Related Decline of Body Movement,and Increased Heat Stress-Resistance

One goal of aging study is to identify drugs that can slow aging and delay age-related illness and degeneration. To identify compounds that might slow aging and extend lifespan in C. elegans, we assayed a panel of compounds with pharmacological effects or bioactivity related to improving health condition or reducing the age-related diseases, such as qingyangshengenin, otophylloside A, otophylloside B and b-sitosterol, which were isolated from C. otophyllum. Among those examined, we found that Ot B (Fig. 1a) could extend the lifespan of C. elegans. Doseresponse analyses indicated that 50 lM of Ot B displayed the largest lifespan extension by up to 11.3 % (Fig. 1c, d). Animals exposed to either higher or lower than 50 lM of Ot B exhibited a smaller or an insignicant lifespan extension (Fig. 1b, c).

The body movement of C. elegans is the one of most obvious behavioral abnormality accompanying nematode aging [18]. We investigated if Ot B could delay the age-related decline of body movement. In both treated and non-

treated animals, body movement declined progressively during aging. However, Ot B treated animals exhibited signicantly lower decline of body movement than non-treated controls (Fig. 2a).

Caenorhabditis elegans with extended lifespan often presents increased stress resistance [19]. So we examined the effect of Ot B on the lethality of heat stress. As shown in Fig. 2b, Ot B treatment suppressed the lethality of heat stress in wild-type C. elegans.

Altogether, by a combination of assays of lifespan, body movement, and heat stress, we showed the anti-aging effect of otophylloside B in C. elegans.

2.2 Ot B Extends the Lifespan of C. elegans Through FOXO Transcription Factor DAF-16

Caenorhabditis elegans DAF-16, the homolog of mammalian Forkhead box O transcription factors (FOXO), was crucial in mediating stress resistance and longevity [2, 17, 20]. We tested if DAF-16 played a role in lifespan extension by Ot B. Our result showed that Ot B could not extend the lifespan of daf-16 null mutant daf-16(mu86) I [21] (Fig. 3a).

To further examine that Ot B did act on DAF-16, we examined the effect of Ot B on the expression of sod-3 by qRT-PCR, which is a known DAF-16 target gene involved in both stress resistance and longevity [22, 23]. The expression of sod-3 was signicantly increased when wild type N2 worms exposed in 50 lM of Ot B for 24 h (Fig. 3b). Besides, Ot B could not increase the expression of sod-3 in daf-16 null mutant exposed in Ot B for 24 h (Fig. 3b). These results suggested Ot B-induced lifespan extension depended on DAF-16.

Previous studies have shown that DAF-16 accumulates in the nucleus when the activity of its upstream kinases is reduced [2]. Thus, we examined whether Ot B could trigger the nuclear localization of DAF-16. In agreement with our model, we found Ot B increased the level of nuclear-localized DAF-16::GFP fusion after 24 h of treatment with Ot B (Fig. 3c), suggesting Ot B treatment might promote DAF-16 activation.

2.3 Effect of Ot B on Lifespan Extension may be Conferred by a Reduction in Insulin/IGF-1-Like Signaling

Daf-16 was known to receiving several upstream inputs to regulate their downstream effects, such as IIS pathway and the silent information regulator 2 (SIR2) [24]. To gain insight into the function of Ot B in lifespan extension, we examined the effect of Ot B on lifespan of the long-lived insulin-like

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Fig. 1 Otophylloside (Ot B) extended adult lifespan in C. elegans. a Chemical structure of Ot B. b Survival curves of wild-type (N2) animals raised at 20 C on nematode growth media plates containing either no Ot B or different concentrations of Ot B(5, 20, 50, 200, and 500 lM). c Doseageresponse analysis of Ot B. Wild-type (N2)

animals was treated with 5, 20, 50, 200, and 500 lM Ot B. The average percentage change in lifespan of at least two independent

experiments was plotted as a function of dosage. d Survival curves of wild-type (N2) animals raised on 0 (black) and 50 lM (red) Ot B plates at 20 C. All experiments the treatments were initiated from the rst day of adulthood and continued until death. Statistical details and repetition of this experiment are summarized in Table S1 (Supplementary information)

receptor mutant daf-2(e1370) III [25]. Our results showed that Ot B could not lengthen the lifespan of daf-2 mutant (Fig. 4a). Thus, effect of Ot B on lifespan extension might be conferred by insulin/IGF-1-like signaling.

While previous study indicated that IIS signaling inhibits the activation of DAF-16, as well as SKN-1, which was both functionally and structurally related to mammalian Nrf transcription factor [26]. The C. elegans SKN-1 was shown to play a role in lifespan extension [2628]. Thus, we investigated whether SKN-1 mediated the lifespan extension by Ot B. We found that Ot B could further increase the mean lifespan of skn-1(zu67) IV (Fig. 4b), suggesting that SKN-1 did not play a role in Ot B-induced lifespan extension.

2.4 Ot B-Induced Lifespan Extension Requires SIR-2.1 but was Independent of eat-2 Minic Dietary Restriction Mechanism

The silent information regulator 2 (SIR2), a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, could bind to DAF-16 and extend the lifespan of C. elegans [29 31]. We investigated if Ot B could act on SIR-2.1 to extend the lifespan of C. elegans with a null mutant strain sir-2.1(ok434) IV [32]. Ot B could not increase the lifespan of sir-2.1 mutant (Fig. 5a), indicating that SIR-2.1 is necessary for Ot B-mediated lifespan extension.

Previous studies have reported SIR-2.1 was a key mediator of the benecial effects of dietary restriction, which is able to

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Fig. 2 Otophylloside (Ot B) delayed age-associated body movement changes and improved thermotolerance. a Age-related movement of worms non-treated and treated with 50 lM Ot B. The movement was assorted as fast movement and not fast movement. The mean fast body movement span was identied as a phenotype of age, Table S3 (supplementary information). b The survival percentage of WT worms

cultured at 35 C non-treated and treated with 50 lM Ot B. The gures showed the mean lifespan of at least three independent experiments, and error bars represented standard deviation (SD). In each experiment, the P values were calculated by KM method log rank test and P \ 0.001. The statistical details are summarized in the

Table S2 (Supplementary information)

Fig. 3 Otophylloside (Ot B) extended adult lifespan in a DAF-16-dependent manner. a Survival curves of daf-16 mutants grown on 0 (black) and 50 lM (red) Ot B plates at 20 C, Ot B could not further extend mean lifespan of daf-16 mutants. In all cases, these data represent the results of a single trial. Repetition of this experiment and statistical details are summarized in Table S1 (supplementary information). b sod-3 mRNA was increased 1.4-

fold in treated with 50 lM Ot B worms compared with non-treated worms. The results presented correspond to the mean and SEM of two independent experiments: **P = 0.007643 in t test. c Ot B could cause DAF-16 nuclear localization. DAF16::GFP-expressing worms were placed on 0 and 50 lM Ot B plates at 20 C for 24 h.

RNA interference treatment by feeding of akt-1 was performed as a positive control

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Fig. 4 Lifespan extension by Otophylloside (Ot B) was mediated by IIS signaling pathway. Survival curves of a daf-2(e1370) III, b skn-1(zu67) IV grown on plates containing 0 (black) and 50 lM (red) Ot

B at 20 C. Ot B could not further extend the lifespan of daf-2(e1370) III, but could extend the lifespan of skn-1(zu67) IV. All drug

treatments were initiated from the rst day of adulthood and continued until death. In all cases, these data represent the results of a single trial. Repetition of this experiment and statistical details are summarized in Table S1 (supplementary information)

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Fig. 5 Otophylloside (Ot B)-related lifespan extension required for SIR-2.1 and reduction of mitochondrial respiration, but could not further extend the lifespan of eat-2. Survival curves of a sir-2.1(ok434) IV, b eat-2(ad1116) II, c clk-1(e2519) III. Ot B could not further extend mean lifespan of sir-2.1(ok434) IV and clk-1(e2519)

III, but could further extend lifespan of eat-2. In all cases, these data represent the results of a single trial. Repetition of this experiment and statistical details are summarized in Table S1 (supplementary information)

integrate sensing of the metabolic status with adaptive transcriptional outputs [33, 34]. The pharyngeal pumping defective mutant eat-2(ad1116) II was considered DR-constitutive for its reduced food intake and extended lifespan [35]. Treatment with 50 lM of Ot B at 20 C could further increase the lifespan of eat-2(ad1116) II, suggesting that Ot B might not act through a eat-2 minic DR mechanism (Fig. 5b).

2.5 Ot B Might Extend Lifespan by Mediating to Reduce Mitochondrial Respiration

Oxidative stress is a crucial factor that inuences aging. Mitochondria respiration plays a major role in reactive

oxygen species production and has been reported to mediate aging process [35, 36]. The mutation of mitochondrial respiration components, such as clk-1, the enzyme in ubiquinone synthesis, was shown to reduce the oxygen consumption. The clk-1 mutant clk-1(e2519) III has the defect of mitochondria respiration and were long-lived [36, 37]. Ot B treatment could not further extend the lifespan of mutants clk-1(e2519) III, indicating that Ot B might extend lifespan by mediating to reduce mitochondrial respiration (Fig. 5c).

In C. elegans, DAF-16 was the main effecter of the well-characterized IIS signaling pathway [22, 25]. Ot B promoted DAF-16 nuclear localization (Fig. 3c), and increased the

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expression level of DAF-16 regulated gene sod-3(Fig. 3b), and could not extend the lifespan of the daf-16 null mutant daf-16(mu86) I (Fig. 3a). Above results suggested that Ot B might act in IIS signaling pathway to extend lifespan. This hypothesis was consistent with our results that Ot B could not further extend the lifespan of the long-lived daf-2 mutant (Fig. 4a). But the lack of a negative effect of Ot B on the other mutants could not rule out the possibility that Ot B might act on other pathways upstream of DAF-16 [38]. We also found Ot B failed to increase the lifespan of sir-2.1 (Fig. 5a) and clk-1 (Fig. 5c). Therefore, these results suggested that Ot B-induced lifespan of C. elegans was not specic to reducing IIS activity, but also depended on other pathways, such as SIR-2.1 and mitochondrial respiration.

Based on activation effect of Ot B on DAF-16, here we considered that there are three possibilities to explain this nding. One possibility is that Ot B directly binds DAF-16 and promotes DAF-16 nuclear localization. The second possibility is that Ot B acts on the protein that functions upstream of DAF-16 in a linear pathway, such as daf-2 or age-1, to activate DAF-16. The third possibility is that the effects of Ot B on DAF-16 indirectly acted on proteins whose function is far upstream of the IIS signaling pathway, and simultaneously acted on protein in parallel to the IIS signaling pathway to regulate DAF-16. To understand the specic mechanism that the Ot B activates DAF-16 to extend the lifespan, further work is required to conrm our expectation.

3 Conclusion

Ot B, a C-21 steroidal glycoside, was isolated from C. otophyllum in 1986 and reported to have bioactivity of anti-epilepsy [16], but the molecular mechanism remains unclear. Our results suggest that 50 lM Ot B extend the lifespan of C. elegans by up to 11.3 %, delay the age-related decline of body movement and improve the stress resistance. Further investigating the molecular mechanism of lifespan extension effect revealed that Ot B could activate the FOXO transcription factor DAF-16 and Ot B could not further extend the lifespan of long-lived mutant of insulin/IGF-1-like receptor (daf-2). Besides, Ot B also requires SIR-2.1 and CLK-1 which is an enzyme in ubiquinone synthesis, for lifespan extension.

4 General Experimental Procedures

4.1 Chemicals and Strains

All stains were obtained from Caenorhabditis Genetics Center (CGC). The following strains were used in this study: wild type N2, DA1116 eat-2(ad1116) II, VC199 sir-

2.1(ok434) IV, CB4876 clk-1(e2519) III, CB1370 daf-2(e1370) III, TJ356 daf-16(zls356) IV, CF1038 daf-16(mu86) I, and EU1 skn-1(zu67) IV. All strains grew and maintained on NGM plates seeded with Escherichia coli OP50 at 20 C.

Ot B was dissolved in DMSO for storage and diluted in PBS while in use. NGM plates containing various concentrations of Ot B were equilibrated overnight before use. The nal DMSO concentration was kept at 0.1 % after with or without adding drugs to the plates.

4.2 Lifespan Assay

Strains were cultured for 2-3 generations before using for lifespan analysis. Lifespan assay were conducted as described previously [15]. In brief, late L4 larvae or young adults were transferred to NGM plates containing inactivated OP50 (65 C for 30 min) and 40 lM of 5-uoro-20-

deoxyuridine (FUDR, Sigma) and scored every day. Animals were transferred to fresh plates with or without drugs every 2-4 days. Each experiment was repeated at least once. Means, SEM, and P value were shown in Table S1.

4.3 Thermo-Tolerance and Body Movement Analysis

In thermo-tolerance assay, day 5 C. elegans were transferred to and maintained on plates with (50 lM) or without

Ot B at 35 C [39]. Live animals were scored the same way as in lifespan assays. At least 50 animals were used for each experiment. Statistical signicance was determined by log-rank (Mantel-Cox) test.

Body movement assay was conducted as described previously [15, 18]. Briey, for each experiment, at least 50 synchronized L4 larvae or young adult worms were treated and maintained as described in the lifespan assays. When tapping the plate, the animals moving in a continuous coordinated sinusoidal way were classied as fast movement.

4.4 DAF-16:: GFP Localization Assay

DAF-16:: GFP localization assay were performed as described previously [40]. Briey, 30 Synchronized young adult worms of transgenic strain TJ356 daf-16(zls356) IV [2] were transferred to the plates with (50 lM) or without

Ot B, and cultured for 12-24 h at 20 C, then monitored DAF-16::GFP signal by a uorescent microscope system (Olympus, IX51).

4.5 RNA Interference Experiments

In experiments before treatment, synchronized and grow to Late L4 larvae or young adults on NGM plates with OP50 were transferred to freshly-prepared NGM plates contained

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gene-specic RNAi bacteria. HT115 bacteria transformed with RNAi vectors (L4440) expressing dsRNA of the genes indicated were grown at 37 C in LB with 100 lg mL-1

ampicillin, then seeded onto NGM plates containing 100 lg of 0.1 M IPTG.

4.6 Quantitative RT-PCR Assay

Quantitative RT-PCR assay were carried out as described previously [15]. Briey, Synchronized young adult wild-type worms cultured with (50 lM) or without Ot B at 20 C for 24 h. Then, total RNA was extracted using RNAiso Plus (Takara) and converted to cDNA with High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). The cDNA of sod-3 was amplied and quantied in a Power SYBR Green PCR Master Mix (Applied Biosystems) on ABI 7500 DNA analyzer (Applied Biosystems). The experiments were conducted in triplicate and the results were carried out using 2-44CT

method, and normalized to cdc-42.

Acknowledgments This work was partially supported by the Yunnan provincial government (20080A007), and the 100 Talents Program of the Chinese Academy of Sciences, the State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany (P2008-ZZ21 and T2009-KF05). We thank the Caenorhabditis Genetic Center (CGC) for providing the worm strains.

Conict of interest The authors declare no conict of interest.

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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