Effect of Colombian strains of Steinernema colombiense (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) against Eurhizococcus colombianus (Hemiptera: Margarodidae) and Aeneolamia sp. (Hemiptera: Cercopidae)

Headnote

Summary

Two entomopathogenic nematodes, Steinernema colombiense sp. nov. (strain SNIO198) (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora Poinar (strain Fresno HNI0100) (Rhabditida: Heterorhabditidae), were isolated from the Colombian Andes and cultured in the laboratory. In semi-field tests against the ground pearl Eurhizococcus colombianus Jakubski (Hemiptera: Margarodidae) on blackberry, neither species was pathogenic to crawlers and only S. colombiense was moderately infective against adults at 105 IJ/ml. Higher activity for both nematodes was observed against immature spittlebugs Aeneolamia sp. (Hemiptera: Cercopidae) infesting kikuyu grass, with H. bacteriophora and S. colombiense killing 100% and 75% of insects, respectively, in our tests. Our results demonstrate the potential use of native strains of entomopathogenic nematodes as biological control agents in Colombia.

Key Words: entomopathogenic nematode; ground pearl; spittlebug; biological control

Sumario

Dos especies nativas de nematodos entomopatógenos, Steinernema colombiense sp. nov. (cepa SNIO198) (Rhabditida: Steinernematidae) y Heterorhabditis bacteriophora Poinar (línea Fresno HNI0100) (Rhabditida: Heterorhabditidae), fueron aisladas de los andes Colombianos y cultivadas en laboratorio. En la evaluación bajo condiciones de semi-campo contra la perla de tierra Eurhizococcus colombianus Jakubski (Hemiptera: Margarodidae) en mora, ninguna de las especies fue patogénica contra los estados inmaduros y solamente S. colombiense fue moderadamente infectiva contra los adultos at 105 IJ/ml. Mayor actividad de ambos entomo nematodos fue observada contra los estados inmaduros del salivazo Aeneolamia sp. (Hemiptera: Cercopidae) infectando pasto kikuyo con H. bacteriophora y S. colombiense controlando en nuestra evaluación 100% y 75% de los insectos respectivamente. Nuestros resultados demuestran el uso potencial de cepas nativas de nematodos entomopatógenos como agentes de control biológico en Colombia.

Palabras Claves: nematodos entomopatógenos; perla de tierra; salivazo; control biológico

A new species of entomopathogenic nematode, Steinernema colombiense sp. nov. (Rhabditida: Steinernematidae), was described from the Central Andean Region of Colombia (López-Núñez et al. 2008). Phylogenetic data placed S. colombiense sp. nov. in the same clade as S. carpocapsae (Weiser), S. monticolum Stock, Choo & Kaya, S. siamkayai Stock, Somsook & Reid, and S. scapterisci Nguyen & Smart, although S. colombiense can be differentiated based on several morphometric features of the infective juvenile (IJ) and the 1st-generation male. Because little information on the use of entomopathogenic nematodes against agricultural pests in Colombia exists, we compared S. colombiense and a native strain of Heterorhabditis bacteriophora Poinar (Rhabditida: Heterorhabditidae), against 2 root-feeding insects that are difficult to control with insecticides.

Related to scale insects, the ground pearl, Eurhizococcus colombianus Jakubski (Hemiptera: Margarodidae), infests fruit crops, such as blackberry Rubus glaucus Benth (Rosales: Rosaceae) (Kondo & Gómez 2008; Rodrígues & Gómez 2008). Immature stages resemble shiny pearl-like cysts, and their feeding results in a decline in host vigor (Foldi 2005). The spittlebug Aeneolamia varia (F.) (Hemiptera: Cercopidae) is an important pest of pastures and sugarcane in the Neotropics (Lucía Cuarán et al. 2012; Gómez 2007). Immature spittlebugs feed on the root sap, whereas adults feed on leaves, injecting a toxin that causes necrosis or "froghopper burn."

Steinernema colombiense (strain SNIO198) and H. bacteriophora (strain Fresno HNIO100) were isolated from the western slopes of the Central Andean highlands (López-Núñez et al. 2007) and reared on Galleria mellonella L. (Lepidoptera: Pyralidae) at the National Coffee Research Center (Cenicafé), Manizales, Colombia, according to the methods of Realpe-Aranda et al. (2007). Experiments were conducted at "Tesorito" farm at the University of Caldas, Manizales, Colombia. The farm was 2,280 m asl, with an average temperature of 17 °C, 180 cm annual rainfall, and 78% RH.

In the 1st experiment, reproductive branches of blackberry were propagated vegetatively by "tip layering" bushes in 2 L plastic containers filled with sterile potting mix. The mix was fumigated with Dazomet (Bazamid® 98%, BASF Corp., Bogotá, Colombia) at 35 g/m2 applied 60 d before propagation. After 30 d, when branches had developed roots and new foliage, they were cut from the mother plant. To establish infestations, 6 adult ground pearls per container (collected from nearby blackberry crop) were placed 5 cm underground, close to the roots. After 30 d, roots were examined to confirm that ground pearls had started reproducing, and populations were standardized with 4 adult females and 10 first instars (crawlers) per plant. Any additional insects were removed with a paintbrush, and soil was replaced around the roots.

Three concentrations of nematodes (103, 104, and 105 IJ/mL) were prepared according to methods of Kaya & Stock (1997). Nematodes were applied as a drench in 500 mL sterile water per container. Nematode viability (> 95% activity) was confirmed under a microscope. Controls received 500 mL water. An insecticide standard consisted of 5 g carbofuran (Furadan® 3G, Bayer CropScience, Bogotá, Colombia) per plant. There were 5 replications per treatment conducted under semi-field conditions, i.e., plants were maintained outside at 15 to 20 °C, 70 to 80% RH, and 5.5 cm of rainfall during the observation period. After 5 d, roots were removed and ground pearl survivorship quantified in the laboratory. Mobility and response to stimulation with a paintbrush (leg movement) were used to assess individuals. Dead individuals were placed in White traps (White 1927) and maintained at 26 °C for 48 h in darkness to observe nematode reproduction.

For the 2nd experiment, stolons of kikuyu grass, Pennisetum clandestinum Hochst. ex Chiov. (Poales: Poaceae) were planted in 1 L pots filled with sterile potting mix and placed in a greenhouse (average 19 °C, 80% RH, and a 10:14 h L:D photoperiod). After 30 d, when new roots and foliage had developed, plants were infested with immature Aeneolamia sp. spittlebugs collected from nearby kikuyu grass. Five nymphs (2nd and 3rd instars) were placed per pot. Nematode treatments were applied after 1 wk when the spittlebugs were feeding. Treatments were S. colombiense and H. bacteriophora applied at 105 IJ/mL in a 300 mL drench. There were 6 replicates, and control plants were treated with water. Insect mortality was assessed after 12 d, based on response to stimulation via paintbrush. Dead spittlebugs were placed in a White trap (25 °C for 20 d in darkness) to evaluate nematode reproduction.

Data from both experiments were evaluated using analysis of variance (ANOVA) with means separated via Tukey's HSD test at P ≤ 0.05. Percentage mortality was corrected with the Schneider-Orelli formula (Schneider-Orelli 1947).

Results showed significant treatment effects for immature E. colombianus ground pearls (F = 167.5, df = 7, P < 0.0001) due to carbofuran, whereas no mortality was observed from nematodes 5 d post-treatment (Table 1). Treatment differences were observed against adults (F = 6.8, df = 7, P < 0.0001). Here, significant mortality was caused by the high rate of S. colombiense, whereas carbofuran was relatively less effective. Mortality of adults exposed to H. bacteriophora strain Fresno was not significantly different from controls, suggesting that this strain was less pathogenic than S. colombiense. Nematode reproduction was corroborated using White traps where IJ progeny were recovered, though not quantified.

In the 2nd experiment, both nematodes induced mortality of immature spittlebugs under greenhouse conditions (F = 252.1, df = 2, P < 0.001) (Fig. 1). The parasitism for S. colombiense was > 70% but significantly lower compared with H. bacteriophora, which killed all spittlebugs by 12 d post-inoculation. Nematode reproduction from White traps was also higher for H. bacteriophora (415 ± 24 IJ per nymph) compared with S. colombiense (37 ± 3 IJ per nymph). Spittlebugs from controls did not show symptoms of parasitism.

To date, few studies have investigated the susceptibility of ground pearls to insect pathogens. Due to their feeding behavior on roots, we hypothesized that E. colombianus might be a good target for entomopathogenic nematodes that naturally occur in soil environments. In our study, only moderate control of the non-feeding adults was achieved with S. colombiense at the highest tested rate, whereas H. bacteriophora was ineffective. The latter result was unexpected because H. bacteriophora is a "cruiser" known to be active within the soil profile (Grewal et al. 1994a). The cooler conditions during tests may have been unsuitable for H. bacteriophora (Grewal et al. 1994b). The foraging behavior of S. colombiense has not been reported. It may be that E. colombianus was not attractive to these nematodes, or that the IJ stages had difficulty penetrating the waxy cysts.

Much better control by both nematodes, especially H. bacteriophora, was achieved against spittlebug nymphs. The relative superiority of H. bacteriophora over several Steinernema species against the sugarcane spittlebug (A. varia) nymphs was noted in 2 previous studies (Moreno Salguero et al. 2012; Rosero-Guerrero et al. 2012). These authors suggested that the "tooth" possessed by Heterorhabditis species (Bedding & Molyneux 1981) may allow them to better penetrate the cuticle of the spittlebug compared with Steinernema species, which lack the tooth.

Information on native entomopathogenic nematodes in Colombia is still scarce. As several distinct geographical regions are represented, further surveys may reveal additional species that might be adapted and developed for biological control strategies against important agricultural pests. We thank National Coffee Research Center, Cenicafé, for supplying nematodes and University of Caldas for providing facilities.

References

References Cited

Bedding RA, Molyneux A. 1981. Penetration of insect cuticle by infective juveniles of Heterorhabditis spp. (Heterorhabditidae: Nematoda). Nematologica 28: 354-359.

Foldi I. 2005. Ground pearls: a generic revision of the Margarodidae sensu stricto (Hemiptera: Sternorrhyncha: Coccoidea). Annales de la Société Entomologique de France 41: 81-125.

Gómez LA. 2007. Manejo del salivazo Aeneolamia varia en cultivos de caña de azúcar en el valle del río Cauca. Carta Trimestral 29: 10-17.

Grewal PS, Lewis EE, Gaugler R, Campbell JF. 1994a. Host finding behaviour as a predictor of foraging strategy in entomopathogenic nematodes. Parasitology 108: 207-215.

Grewal PS, Selvan S, Gaugler R. 1994b. Thermal adaptation of entomopathogenic nematodes: niche breadth for infection, establishment, and reproduction. Journal of Thermal Biology 19: 245-253.

Kaya HK, Stock SP. 1997. Techniques in insect nematology, pp. 281-324 In Lacey LA [ed.], Manual of Techniques in Insect Pathology. Academic Press, London, United Kingdom.

Kondo T, Gómez CE. 2008. La perla de tierra, Eurhizococcus colombianus Jakubski, una nueva plaga de la vid, Vitis labrusca L. en el Valle del Cauca, Colombia. Novedades Técnicas 10: 34-40.

López-Núñez JC, Cano L, Góngora CE, Stock SP. 2007. Diversity and evolutionary relationships of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from the Central Andean Region of Colombia. Nematology 9: 333-341.

López-Núñez JC, Plichta K, Góngora-Botero CE, Stock P. 2008. A new entomopathogenic nematode, Steinernema colombiense n. sp. (Nematoda: Steinernematidae) from Colombia. Nematology 10: 561-574.

Lucía Cuarán V, Castro Valderrama U, Bustillo Pardey AE, Mesa Cobo NC, Ramírez Sánchez GD, Moreno Gil CA, Gómez Laverde LA. 2012. Método para evaluar el daño de los salivazos (Hemiptera: Cercopidae) sobre caña de azúcar, Saccharum spp. Revista Colombiana de Entomología 38: 171-176.

Moreno Salguero CA, Bustillo Pardey AE, López Núñez JC, Castro Valderrama U, Ramírez Sánchez GD. 2012. Virulencia de nematodos entomopatógenos para el control del salivazo Aeneolamia varia (Hemiptera: Cercopidae) en caña de azúcar. Revista Colombiana de Entomología 38: 260-265.

Realpe-Aranda FJ, Bustillo-Pardey AE, López-Núñez JC. 2007. Optimización de la cría de Galleria mellonella (L.) para la producción de nematodos entomopatógenos parásitos de la broca del café. Cenicafé 58: 142-157.

Rodrígues DTK, Gómez CE. 2008. La perla de tierra, Eurhizococcus colombianus Jakubski, una nueva plaga de la vid, Vitis labrusca L. en el Valle del Cauca, Colombia. Novedades Técnicas, Centro de Investigación Palmira 9: 34-40.

Rosero-Guerrero M, Bustillo-Pardey AE, López-Núñez JC, Castro-Valderrama U, Gómez-López ED. 2012. Eficacia de entomonematodes para controlar estados de Aeneolamia varia (Hemiptera: Cercopidae) bajo condiciones de invernadero. Revista Colombiana de Entomología 38: 266-273.

Schneider-Orelli O. 1947. Entomologisches Praktikum: Einführung in die landund forstwirtschaftliche Insektenkunde. Sauerländer & Co., Aarau, Switzerland.

White GF. 1927. A method for obtaining infective nematode larvae from cultures. Science 66: 302-303.