Alta correlacion en la deteccion de anticuerpos y antigenos de virus del dengue en muestras de suero y plasma

Abstract

Introduction. Appropriate dengue laboratory diagnosis is an important tool to manage the disease, as well to control possible outbreaks. Currently, ELISA dengue serological tests are validated to use with serum, however sometimes samples quality or quantity is inappropriate or there is only availability of anti-coagulated blood samples which have been used in total blood tests haemogram.

Objective. To assess the performance of four dengue ELISA tests in serum or plasma samples obtained from presumptive dengue cases.

Methodology. Forty two samples, 21 of serum and plasma each, were processed to dengue Capture IgM ultramicro-ELISA, Capture IgG ELISA, Indirect IgG ELISA and NS1 antigen ELISA. Nine out of 21 patients were diagnosed as dengue cases following the diagnostic algorithm.

Results. The percentage of positive samples found was Capture IgM 33.3%, Capture IgG 33.3%, Indirect IgG 90.5% and NS1 dengue antigen 23.8%. Comparing the results between all ELISA tests it can be said that they had similar performances both in serum and plasma, the Kappa coefcient obtained was 1.0.

Conclusions. These results show a high concordance between the measurements carried out in serum and plasma, which leads to suggest the latter may be used as a tool in the diagnosis and conrmation of dengue cases.