It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
Although zebrafish (Danio rerio) and medaka (Oryzias latipes) are primitive vertebrates, they have several advantages over other model animals. For example, they are fecund and light can control their ovulation. Spawning takes place frequently and no limitation in their spawning season. Microinjection of fertilized eggs is easily accessible and relatively cheap. Their embryos are transparent, making it easy to monitor the dynamic gene expression in various tissues and organs in vivo without the need to sacrifice the experimental subjects. Their genome sizes are approximately 20 to 40 % of the mammalian genome, making them the only vertebrates available for large-scale mutagenesis. Their maturation time takes only 2?~?3 months, which is relatively less laborious and time-saving for generating transgenic lines. In addition, many routine techniques of molecular biology and genetics, including knock-in, knockdown and knockout, are well developed in the model fish. Therefore, zebrafish and medaka are new excellent animal systems for the study of vertebrate-specific biology in vivo.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer