A novel fusion of SQSTM1 and FGFR1 in a patient with acute myelomonocytic leukemia with t(5;8)(q35;p11) translocation

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Citation: Blood Cancer Journal (2014) 4, e265; doi:http://dx.doi.org/10.1038/bcj.2014.86

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LETTER TO THE EDITOR

A novel fusion of SQSTM1 and FGFR1 in a patient with acute myelomonocytic leukemia with t(5;8)(q35;p11) translocation

Blood Cancer Journal (2014) 4, e265; doi:http://dx.doi.org/10.1038/bcj.2014.86

Web End =10.1038/bcj.2014.86 ; published online 12 December 2014

Hematological malignancies with FGFR1 abnormality (8p11 myeloproliferative syndrome; EMS) are rare atypical stem cell disorders characterized by eosinophilia, T-cell proliferation and progression to acute myeloid leukemia.1,2 In EMS, broblast

growth factor receptor 1 (FGFR1) gene at 8p11 is disrupted by chromosomal translocation, resulting in the formation of chimeric products with various partner genes. To date, 13 FGFR1 partners have been identied: ZMYM2 (ZNF198) at 13q12; CNTR at 9q33; FGFR1OP at 6q27; BCR at 22q11; HERVK at 19p13.3; FGFR1OP2 at 12p11; TRIM24 (TIF1) at 7q34; CPSF6 at 12q15; MYO18A at 17q23; LRRFIP1 at 2q37; CUX1 at 7q34; TPR at 1q25; and RANBP2/NUP358 at 2q12.25 In all reported cases in which chimeric transcripts were cloned, N-terminal portion of the predicted fusion protein is composed of a partner gene with a dimerization domain, which may induce the constitutive activation of FGFR1 tyrosine kinase in the C-terminal portion, leading to the cellular transformation.2

Here, we report the identication of sequestosome 1 (SQSTM1) gene at 5q35 as a novel fusion partner of the FGFR1 in a patient with acute myelomonocytic leukemia presenting 8p11 chromosomal abnormality.

A 76-year-old man was referred to our hospital for anemia with hemoglobin level of 5.0 g/dl. The white blood cell count was4.1 109 cells/l with 11% blasts, 1% eosinophils, 29% neutrophils and 30% monocytes, Bone marrow (BM) was hypercellular with 43% blasts and 17% monocytes. Dysplastic changes were observed in peripheral neutrophils and BM megakaryocytes. Immunphenotyping revealed that leukemic cells were positive for CD13, CD33, CD34 and HLA-DR and negative for other myeloid and lymphoid markers. He was diagnosed as acute myelomonocytic leukemia and received blood transfusion therapy for anemia, but chemotherapy was not done because of poor performance status. He died of progressive disease 15 months after the diagnosis.

Chromosome analysis of the BM cells showed the karyotype as 46,XY,t(5;8)(q35;p11) (20/20 cells) (Figure 1a). To characterize the 8p11 translocation, Southern blot analysis of DNA from the patients peripheral leukemic cells was performed using a genomic probe containing FGFR1 exon 9 and 10, as chromosomal breaks occur within intron 8 in most cases with EMS. Abnormal bands were observed in sample with BamHI or HindIII digestion (Figure 1b), conrming that FGFR1 was rearranged by the translocation.

To determine the FGFR1 partner gene, we searched for a FGFR1 chimeric transcript by 5-rapid amplication of cDNA end (RACE)

method using 5-Full RACE core set (Takara Bio, Otsu, Japan). Total RNA was extracted from the leukemic cells and the rst cDNA strand was synthesized with phosphorylated primer in FGFR1 exon 12 (5-GCCAGATACTCCATGCCTCG-3) by reverse transcription.

After ligation, cDNA was amplied by PCR with FGFR1-10R1 (5-ACACCACCTGCCCAAAGCAGC-3) and FGFR1-10F1 (5-AGGCT

ATCGGGCTGGACAAGG-3) primers in exon 10. The PCR product was further amplied with FGFR1-10R2 (5-CAGGGGTTTGCCTAA GACCAG-3) and FGFR1-10F2 (5-CGTGTGACCAAAGTGGCTGTG-3)

primes in exon 10. An abnormal band was amplied and sequencing of the PCR product revealed that FGFR1 was fused to a 5 foreign sequence that was identied as exon 9 of SQSTM1 at chromosome 5q35, showing that SQSTM1 was juxtaposed to FGFR1 as the result of the chromosomal translocation.

To ascertain the formation of chimeric transcript, we performed reverse transcription PCR (RT-PCR) analysis, using SQSTM1-8F1 (5-CCTGAAGAACGTTGGGAGAG-3) for exon 8 and FGFR1-10R1 primers. An amplied product was obtained from the patients leukemic cells (Figure 1c). Sequencing analysis of the PCR product revealed that the SQSTM1 exon 9 was fused to FGFR1 exon 9 (Figures 1c and d).

A reciprocal FGFR1-SQSTM1 fusion transcript was also detected in using forward FGFR1-7F1 (5-GTAACTCTATCGGACTCTCCC-3) for exon 7 and reverse SQSTM1-11R1 (5-CGGGGGATGCTTTGAATA CTGG-3) for exon 11 primes (Figures 1c and d).

To identify the chromosomal breakpoints, genomic DNA was amplied by the long-and-accurate PCR method using LA Taq polymerase (Takara Bio). The following primers were used: FGFR1-7F1 and FGFR1-7F2 (5-TCTGCATGGTTGACCGTTCTG-3) for exon 7 outer and inner; FGFR1-10R1 and FGFR1-10R2 for exon 10 outer and inner; SQSTM1-9F1 (5-GATATCGATGTGGAGCACGGAGGG-3),

SQSTM1-9F2 (5-CTCCAGAGAGTTCCAGCACAGAGG-3) for exon 9 outer and inner; and SQSTM1-10R1 (5-TGTGGGTACAAGGC AGCTTCC-3), SQSTM1-10R2 (5-CTTGGCCCTTCGGATTCTGGC-3) for exon 10 outer and inner, respectively. Genomic fragments corresponding to the der(5) and der(8) chromosomes were amplied by primers around the predicted breakpoints on both chromosomes (Figures 1e and f). The sequence analysis of the PCR products identied the chromosome 5 and 8 breakpoints within SQSTM1 intron 9 and FGFR1 intron 9, respectively (data not shown).

As sequence databases contain alternative transcripts for SQSTM1 coding N-terminal truncated protein (NCBI Reference Sequence: NM_001142298.1 and NM_00114299.1), we performed RT-PCR using primers located in SQSTM1 alternative exons 1, 2 or 4 and FGFR1 exon 9 and detected only a chimeric transcript from SQSTM1 exon 4 coding the full N-terminal end of SQSTM1 (data not shown).

SQSTM1 at chromosome 5q35 encodes a multifunctional protein that binds ubiquitin and regulates activation of the NF-kB signaling pathway, associating with oxidative stress and autophagy.69 Mutations in this gene were reported to cause Paget disease of bone.10 The SQSTM1-FGFR1 transcript encodes a predicted chimeric protein of 718 amino acids, containing the N-terminal protein-protein interaction domain, PB1, of SQSTM1 (ref. 11) and the tyrosine kinase domain of FGFR1 (Figure 2). SQSTM1-FGFR1 may induce the constitutive activation of tyrosine kinase by PB1-mediated dimerization, thus leading to the cellular transformation.

Recently, the generation of SQSTM1-NUP214 and SQSTM1-ALK fusion genes were reported in one case with adult T-cell acute lymphoblastic leukemia presenting t(5;9)(q35;q34) translocation and two cases with anaplastic lymphoma kinase (ALK)-positive large B-cell lymphoma, respectively.1214 SQSMT1-ALK was shown to possess transforming activity in 3T3 broblasts, probably by the constitutive activation of ALK tyrosine kinase by PB1-mediated

Letter to the Editor

2

SQSTM1 exon 9

TTGGAGTCCGAGGGGCGCCCTGAG L E S E G R P E

FGFR1 exon 8

FGFR1 exon 9

GTGTCTGCTGACTCCAGTGCATCC

SQSTM1 exon 10

GAACAGATGGAGTCGGATAACTGT

ATCCCTCTGCGCAGACAGGTAACA

I P L R R Q V T

V S A D S S A S

E Q M E S D N C

C P

C P

C P

(kb)

2.0

23

9.46.74.32.2

der(8)

8.8kb

FGFR1-SQSTM1

SQSTM1 FGFR1

-

8p11

exon 7 8 9 10

10

2.4kb

C P

FGFR1

BamHI

HindIII

exon 8

9

9 10

der(5)

SQSTM1-FGFR1

FGFR1-SQSTM1 GAPDH

C P C P

C P

exon 7 8

10

(bp)

612 495

1057

770

341

5q35

9

SQSTM1

exon 8

Figure 1. Cytogenetic and molecular analysis. (a) Partial karyotyping of patients bone marrow cells. The derivative chromosome 5 and 8 are shown by arrows. (b) Southern blot analysis, presenting rearrangement within FGFR1 gene. Arrows indicate the abnormal bands. (c) RT-PCR detection of the fusion transcripts in the patient. (d) Partial sequence of SQSTM1-FGFR1 and reciprocal FGFR1-SQSTM1 fusion cDNA. The amino-acid translations spanning the fusion are shown under the sequence. (e) Detection of der(5) and der(8) chromosomes in the patients leukemic cells. Amplied genomic DNA fragments from the patients sample are indicated by arrows. (f) Genomic organization of FGFR1 and SQSTM1, encompassing the breakpoints. Horizontal arrows indicate primers used in PCR. Lanes C and P represent normal control and the patients sample, respectively.

FGFR1

SQSTM1

SQSTM1-FGFR1

ZZ finger domain

TRAF6 binding domain

Ig-like domain

Transmembrane domain

Tyrosine kinase domain

PEST

PB1 domain

Figure 2. Schematic representation of FGFR1, SQSTM1 and the predicted SQSTM1-FGFR1 fusion protein. Relevant protein domains are shown. The breakpoints within proteins are indicated by arrows.

UBA (Ub-associated domain)

dimerization.13 To our knowledge, our case is the third instance of hematologic malignancy, in which SQSTM1 was involved in chromosomal translocation. SQSTM1 may be a recurrent target of chromosomal aberration.

Our patient, diagnosed as acute myelomonocytic leukemia, lacked typical EMS features, such as myeloproliferation,

eosinophilia or lymphadenopathy involved by T-cell proliferation. Multilinage dysplasia indicated the leukemic transformation at early myeloid precursor level, but involvement of the T-cell lineage was not determined. SQSTM1-FGFR1 and/or reciprocal FGFR1-SQSTM1 fusion gene may exert to promote proliferation of immature myelomonocytic cells preferentially. Further investigation is needed to clarify this point.

CONFLICT OF INTEREST

The authors declare no conict of interest.

Y Nakamura, Y Ito, N Wakimoto, E Kakegawa, Y Uchida and M Bessho

Department of Hematology, Saitama Medical University, Saitama, Japan E-mail: mailto:[email protected]

Web End [email protected]

REFERENCES

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