Immunogenic tumor cell death induced by chemoradiotherapy: molecular mechanisms and a clinical translation

Abstract

Chemoradiotherapy can induce immunogenic cell death, triggering danger signals such as high-mobility group box 1 protein, and resulting in T-cell immunity. This concept can potentially be harnessed for clinical therapy to enhance tumor-specific immunity. There is however limited information to translate this theory directly in a clinical setting. In this review, we will discuss and summarize molecular and cellular mechanisms underlying immunogenic tumor cell death induced by chemoradiotherapy, with emphasis on a clinical translation.

OPEN

Review

Citation: Cell Death and Disease (2013) 4, e688; doi:10.1038/cddis.2013.207

& 2013 Macmillan Publishers Limited All rights reserved 2041-4889/13 http://www.nature.com/cddis

Web End =www.nature.com/cddis

K Kono*,1,2, K Mimura1,2 and R Kiessling3

Chemoradiotherapy can induce immunogenic cell death, triggering danger signals such as high-mobility group box 1 protein, and resulting in T-cell immunity. This concept can potentially be harnessed for clinical therapy to enhance tumor-specic immunity. There is however limited information to translate this theory directly in a clinical setting. In this review, we will discuss and summarize molecular and cellular mechanisms underlying immunogenic tumor cell death induced by chemoradiotherapy, with emphasis on a clinical translation.

Cell Death and Disease (2013) 4, e688; doi:http://dx.doi.org/10.1038/cddis.2013.207

Web End =10.1038/cddis.2013.207 ; published online 20 June 2013

Subject Category: Cancer

Facts

Immunogenic tumor cell death can elicit uptake of

antigenic components by dendritic cells (DCs) and provide antigenic signals to T cells, resulting in the expansion of antigen-specic cytotoxic T lymphocytes (CTLs) and production of tumor-specic mAbs in murine models.

Chemotherapy and radiotherapy induce danger signals

such as high-mobility group box 1 (HMGB1) protein and immunogenic tumor cell death, which have an important role in harmonizing innate and acquired antitumor immunity.

Chemoradiation induces tumor antigen-specic T-cell

responses in clinical settings, which have the capability of modulating the clinical outcome of malignant diseases.

Chemoradiotherapy can alter the frequency and function of

immune regulatory cells including regulatory T cells (Tregs)

and myeloid-derived suppressor cells (MDSC).

Open Questions

What are the specics of the processes involved in

immunogenic tumor cell death in a clinical setting?

Does HMGB1/toll-like receptor (TLR)4 interaction alone

have a role in immunogenic tumor cell death?

Can we predict clinical responses after radiotherapy or

chemotherapy based on immunogenic tumor cell-death theory by measuring serum HMGB1 levels?

Keywords: HMGB1; calreticulin; immunogenic cell death; chemoradiation; cancer-testis antigen; TIM-3

Abbreviations: HMGB1, high-mobility group box 1; TLR, toll-like receptor; DCs, dendritic cells; CTLs, cytotoxic T lymphocytes; Tregs, regulatory T cells; ROS, reactive oxygen species; MDSC, myeloid-derived suppressor cells; ESCC, esophageal squamous cell carcinoma; TIM-3, T-cell immunoglobulin- and mucin-domain-containing molecule

Immunogenic tumor cell death induced by chemoradiotherapy: molecular mechanisms and a clinical translation

Introduction

It is well established that certain solid tumors respond clinically to radiotherapy, chemotherapy or combinations thereof, which prolong patient survival.1,2 It is also generally

accepted that radiation is able to directly induce apoptosis or necrosis of solid tumors and various strategies have been developed to enhance direct cytotoxic and cytostatic effects through radiotherapy.3,4 A widely recognized, albeit rare phenomenon in clinical radiation oncology is called the abscopal effect,58 in which local radiotherapy causes regression of metastatic cancer at a distance from the irradiated site. The mechanisms underlying this phenomenon are not completely understood.

Recently, there is accumulating evidence to support the novel concept that radiotherapy and/or chemotherapy may induce immunogenic cell death and trigger uptake of antigenic components by DCs, which stimulate antigen-specic CTLs and production of tumor-specic mAbs in murine models.918

It has been shown that the nexus between the direct effects of irradiation and enhanced tumor-specic immunity induced by the irradiation are essential for rejection of inoculated live tumors, where irradiation alone was not able to reject tumors in murine models.9,10 Important mediators that induce immunogenic cell death include HMGB1, calreticulin and fragments of polynucleotides induced by chemoradiation.918

However, there is limited information describing whether immunogenic cell death could be induced by radiotherapy or/and chemotherapy in clinical settings, mainly because of

1Department of Surgery, National University of Singapore, Singapore; 2Cancer Science Institute of Singapore, Singapore and 3Department of Oncology and Pathology, Immune and Gene Therapy Laboratory, Cancer Center Karolinska, Karolinska Institutet, Stockholm, Sweden*Corresponding author: K Kono, Department of Surgery, National University of Singapore, Level 8, NUHS Tower Block, 1E Kent Ridge Road, Singapore 119228, Singapore. Tel: [phone]; Fax: [phone]; Email: mailto:[email protected]

Web End [email protected]

Received 09.4.13; revised 30.4.13; accepted 16.5.13; Edited by M Piacentini

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the lack of accurate assay systems to evaluate antigen-specic T-cell responses in cancer patients receiving chemoradiotherapy. In this review, we will discuss immunogenic tumor cell death induced by chemoradiotherapy, with a particular focus on molecular mechanisms and a clinical setting.

The Abscopal Effect in Radiation Oncology

For decades, external beam irradiation has been utilized as loco-regional therapy for cancer and one of the adverse effects is suppression of systemic T cell- and NK cell-mediated immunity. However, radiation oncologists realized that there was a rare phenomenon called the abscopal effect where local irradiation of a particular tumor site caused regression of metastases at sites distant from the irradiated area.5 This phenomenon was originally described by Dr. RJ Mole in 1953 who coined the name from Latin ab (position away from) and scopus (mark or target). The abscopal effect has been shown in several types of malignant tumors, including melanoma, lymphoma, hepatocellular carcinoma and renal cell carcinoma.68,19 As an example of

the abscopal effect, we have shown a patient with lymphoma who developed the abscopal effect after receiving radiation (Figure 1). Although the underlying mechanisms of the abscopal effect have not been completely understood, radiation oncologists speculated that activation of antitumor immunity in response to irradiation might be one of the mechanisms of to the abscopal effect.20,21 Postow et al.22

recently reported a case of the abscopal effect in a patient with melanoma treated with ipilimimab and radiotherapy, where tumor shrinkage was accompanied with antibody responses to the cancer-testis antigen NY-ESO-1 and changes in peripheral blood immune cells after radiotherapy. On the basis of the patients unforeseen systemic response after local radiotherapy in combination with ipilimimab, they have conducted prospective clinical trials with this combination approach (ipilimimab and radiotherapy) in prostate cancer (NCT00861614) and melanoma (NCT01449279).

Chemoradiotherapy can Induce Alterations in Immune Regulatory Cells

In addition to the direct radiation-induced genetic damage and enhanced cross-presentation of tumor antigens, radiation can also affect immune regulatory circuits. There is evidence for radiation-induced modulation of effector versus CD4

CD25 Tregs ratios,23 and this may have protracted and

profound effects on the composition and function of T-cell populations. The mechanism behind this is unknown, but may involve a differential sensitivity of these T-cell subsets to radiation-induced reactive oxygen species (ROS). This view is compatible with our observation that Tregs are more resistant

to ROS-induced cell death as compared with T effectors, and Tregs maintain their suppressive function under conditions of high oxidative stress including radiation exposure.24 The

molecular mechanism behind this resistance of Tregs to

oxidative stress may be related to the nding that human Tregs express and secrete higher levels of thioredoxin-1, a major antioxidative molecule.25 Radiation-induced immune

suppression could therefore, at least in part, be explained by preferential induction of apoptosis in T-effector cells by ROS and sparing of Tregs through a thioredoxin-dependent mechanism.

Another immune suppressive factor to consider when discussing the effect of chemoradiotherapy on the immune system is the MDSC. They are a heterogenous population of myeloid cells with suppressive activity, containing precursors of granulocytes, macrophages and DCs.26 Circulating MDSC levels are increased in the blood of patients with several types of cancer and can induce profound suppression of T-cell and NK-cell functions. Several chemotherapeutic agents such as gemcitabine and 5-uorouracil can downregulate MDSC frequencies27,28 and may therefore add to their clinical efcacy. One of the potential means of chemoradiotherapy-induced immune modulation may therefore occur through the effect on MDSC.

Tumor Antigen-Specic T-Cell Response Induced By Chemoradiotherapy

With the panel of HLA-A-restricted epitopes identied by us,2932 we have established a reliable in vitro assay system using PBLs to detect tumor-specic CTL responses against

(Before local radiotherapy) (after local radiotherapy)

Left parotid grand invasion

Right hilar lymph node invasion

33x27mm 27x17mm

This case was generously provided by Dr. Yoshiyuki Suzuki (Department of Radiation Oncology, Gunma

University Graduate School of Medicine, Japan)

Figure 1 The abscopal effect in a relapsed lymphoma patient. An 81-year-old female had a relapsed lymphoma. Radiation therapy (total of 30.6 Gy in 17 fractions) was administered to the left hilar lymph node lesion (blue square). FDG-PET images (top and middle in panel a and b) and axial CT images (bottom in panel a and b) are shown. Blue squares indicate the irradiated eld to the left hilar lymph node invasion; black arrows indicate the left parotid gland invasion (middle in panel a and b) and white arrows indicate the right hilar lymph node invasion (bottom in panel a and b). Panel a shows images before local radiotherapy. In panel b showing images at 8 months after local radiotherapy, the radiation-targeted left hilar lymphadenopathy and abnormal accumulation of FDG was disappeared (top). Furthermore, disease response outside of the irradiated eld was seen with decreased left parotid gland (middle) and right hilar lymph node invasion (top and bottom)

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these cancer-testis antigens.2931 Using this assay, we

examined tumor antigen-specic CTL responses in esophageal squamous cell carcinoma (ESCC) patients receiving chemoradiotherapy. In this study, tumor antigen-specic T-cell responses were conrmed in 6 (38%) out of 16 ESCC patients following chemoradiotherapy.32 Furthermore, the level of HMGB1 following chemoradiation in the patients with antigen-specic T-cell responses was signicantly elevated in comparison with that in the patients without antigen-specic T-cell responses.32 These results indicate that chemoradiation could induce tumor antigen-specic T-cell responses in a clinical setting, which correlated to HMGB1 levels (Figure 2). These results are analogous to the observations in several mouse models.

HMGB1/TLR4

HMGB1 is a chromatin-binding protein that acts as a transcription factor when present in the nucleus, as well as a proinammatory cytokine, when released extracellularly from inammatory or dying cells.33 HMGB1 is primarily released from damaged and necrotic cells, but it has been recently reported that late apoptosis is also associated with HMGB1 release.34 Importantly, anticancer treatments such as irradiation, anthracyclines and oxaliplatin induce HMGB1 release into the extracellular milieu by dying tumor cells.915 Once

HMGB1 is released extracellularly, it binds to its receptor, TLR4, which is mainly expressed on DCs. HMGB1 has the ability to promote DC activation and trigger human DC migration in vitro.3538 Subsequently, signaling through TLR4 and its adaptor MyD88 controls the initiation phase of cognate immune responses by regulating the processing of the phagocytosis and the cross-presentation of tumor antigens by DCs on MHC class I and class II molecules.10

HMGB1 released from tumor cells after chemotherapy enhances engulfment of antigenic components by DCs through TRL4 and mediates cross-presentation of tumor antigens into CD4 and CD8 T cells in several murine models.915

In the clinical setting, we have shown that upregulation of HMGB1 within tumor microenvironment was signicantly

related to preoperative chemoradiotherapy and higher levels of local HMGB1 correlated with increased patient survival.32

Moreover, tumor antigen-specic T-cell responses positively correlated with HMGB1 production.32 In addition, Apetoh et al.10 examined TLR4 loss-of-function allele in breast cancer patients, where polymorphism rs4986790 was found to reduce the binding of HMGB1 to TLR4. They reported that patients with TLR4 loss-of-function allele relapsed earlier after chemotherapy and radiotherapy than those with a high-binding TLR4 allele. These observations suggest a clinically relevant immune synapse between HMGB1 and TLR4. Thus, HMGB1-related immune response after chemoradiotherapy may have an important and critical role in clinical outcome of cancer treatments.

Paradoxically, it has been reported that an increased serum level of HMGB1 could predict tumor growth and invasiveness in several types of human cancer, in which elevated serum HMGB1 was linked with poor prognosis of cancer patients.39,40,41 It is likely that serum HMGB1 in these patients

resulted from a systemic inammatory response rather than the tumor microenvironment, as described above. We proposed that HMGB1 has two aspects on its action: systemic responses related to inammatory reactions and local roles such as tumor microenvironment (Table 1, Figure 2).4246

HMGB1 is also known to promote regulatory T-cell activity in cancer-bearing hosts, leading to the progression of preneoplastic lesions to cancer.47,48 Furthermore, HMGB1 is reported to activate TLR4- and RAGE-signaling pathways to induce caspase-1 activation with the subsequent production of multiple inammatory mediators, which in turn promote cancer invasion and metastasis.49

Thus, HMGB1TLR4 interaction appears to be Janus-like in its activity, stimulating immune responses to the detriment

Positive correlation Local HMGB1 (IHC)

Patient survival, response to CRT

ESCC 32

TLR4 polymorphism

Survival after chemoradiotherapy

Breast 10

Chemoradiation

Dying Tumor

calreticulin

RAGE Immature

DC

Inverse correlation

Serum HMGB1 (ELISA)

Patient survival Gastric 39

Serum HMGB1 (ELISA)

Mature

Response to chemoembolization

Liver metastasis

42

DC

Class I

DC

HMGB1 TLR-4

Serum HMGB1 (ELISA)

Patient survival Pancreas 43

Local HMGB1 (qPCR)

Patient survival Colon 44

Local HMGB1 (IHC)

Ag presentation

Patient survival Head/neck 45

CD8

Tumor-antigen specific T cells

Not signicant correlation

Serum HMGB1(ELISA)

Patient survival Colon 46

Abbreviations: CRT, chemoradiotherapy; ESCC, esophageal squamous cell carcinoma; HMGB1, high-mobility group box 1 protein; IHC, immunohisto-chemistry; qPCR, quantitative PCR

; correlation

Figure 2 Schematic representations of tumor antigen-specic T-cell responses in patients with ESCC receiving chemoradiotherapy. HMGB1, high-mobility group box 1 protein

Table 1 Reports on the relationship between HMGB1 status and patients prognosis

HMGB1 evaluation

Clinical signicance Cancer References

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of the tumor or conversely aiding and abetting the growth of the tumor, as summarized in Table 1. The specic context or precise mechanisms by which the opposing effects are prompted remain unelucidated.

Calreticulin

Calreticulin predominantly reside within the lumen of the endoplasmic reticulum where they act as a Ca2-binding lectin chaperone.50,51 Premature exposure of calreticulin on the membrane following radiation enhances phagocytosis of dying tumor cells by DCs in vitro.15,52,53 Calreticulin is related to autophagy-dependent anticancer immune responses induced by chemotherapeutic agents.54 Once exposed on the membrane, the calreticulin/ERp57 complex provides a devour me signal promoting uptake by DC of dying tumor cells.52,55

It has been shown that both HMGB1 release and calreticulin cell surface expression are required for antigen-specic T-cell response in a murine tumor-bearing model.52 However, we did not observe any signicant differences in calreticulin expression between patients with and without chemoradiation, and there was no survival difference between calreticulin-strong and -weak groups. Moreover, the grade of inltrating CD8( ) T cells within tumor microenvironment did not differ

between calreticulin-strong and -weak groups.32 There is thus a discrepancy between the murine model and clinical setting in terms of signicance of calreticulin expression in relation to T-cell immunity or chemoradiotherapy. Kroemer and Zitvogel17 previously reported that membrane expression of calreticulin was more relevant than global intracellular expression when immunogenic tumor cell death occurred in the murine model.52 Further investigation with different methodologies to measure the cell surface expression of calreticulin in clinical samples may resolve the paradox observed between the murine and human studies. Also, more accurate and detailed evaluations of calreticulin in clinical samples provide us with vital information including correlations with survival and a possible predictive marker of clinical effects.

TIM-3 And HMGB1 on Tumor-Inltrating DCs

A recent study described by Chiba et al.56 adds more complexity into the immunogenic cell-death concept by proposing the involvement of T-cell immunoglobulin- and mucin-domain-containing molecule (TIM-3).5658 TIM-3 was

rst identied as a receptor expressed by T-helper type 1 cells and interaction between TIM-3 and its ligand, galectin-9 provides an inhibitory signal that leads to apoptosis of T-helper type 1 cells.5961 Chiba et al.56 reported that tumor-

inltrating DCs expressed higher level of TIM-3 than conventional DCs, and tumor-inltrating DCs suppressed antitumor immune responses through TIM-3-mediated negative regulation of nucleic acid-dependent innate immunity.56 In general, the interaction between galectin-9 and TIM-3 on antigen-presenting cells has an activating role in DC maturation and the cross-priming of tumor-specic T cells.62,63 In contrast,

TIM-3 on tumor-inltrating DCs has an inhibitory effect for nucleic acid-mediated innate immune responses via a

galectin-9-independent but HMGB1-dependent mechanism.56 Chiba et al.56 proposed that TIM-3 on DCs within tumor microenvironment having impaired induction of galectin-9 and high expression of HMGB1 may suppress innate immune responses by interfering with HMGB1 TLR4-mediated stimulation (Figure 3). It is possible that the balance between a positive signal through HMGB1/TLR4 and a negative signal through HMGB1/TIM-3 might regulate the tumor antigen-specic T-cell responses within tumor microenvironment. More detailed evaluations in clinical samples are desirable and inevitable in order to draw a solid conclusion.

Clinical Relevance Regarding to Immunogenic Tumor Cell Death

Chemoradiotherapy is generally thought to be immunosuppressive, either by depleting T- and NK-cells or by rendering them functionally inactive. Systemic immunity in patients receiving chemoradiotherapy is often severely impaired, leading to infectious complications and growth of residual tumors.64,65 Most chemotherapeutic drugs or radiotherapy mediate their cytotoxic effects by the induction of apoptosis, which is generally considered to be noninammatory and nonimmunogenic.66,67 However, it has been proposed that danger signals released by dying cells following chemoradiotherapy could induce HMGB1/TLR4-dependent, antigen-specic T-cell immunity in a clinical setting, as shown in Figures 2 and 3.

Of interest, we showed that chemoradiation could induce upregulation of local HMGB1 with signicant variations among ESCC patients (Figure 2), and patients with high HMGB1 expression had better OS than patients with weak HMGB1 expression.32 Also, the in vitro study indicated that there were substantial variations in HMGB1 production following chemoradiation depending on ESCC cell lines regardless of almost the same amount of dying cells.32 These observations suggest that immune reactions related to HMGB1 production following chemoradiation may affect clinical outcomes in ESCC patients.

Although cancer patients with solid tumor including the esophageal, rectal, lung and gastric cancers are clinically

Tumor-antigen specific T cells

Chemoradiation

RAGE

CD8

Dendritic Cells

Ag presentation

Tumorcells CD4

HMGB1 inhibition

TIM-3

Dying T

HMGB1

TLR-4

Dendritic Cells

Figure 3 Schematic representations of possible molecular machineries whereby TIM-3 on tumor-inltrating DCs negatively regulate innate immune signals that would be activated by HMGB1TLR4 interaction. HMGB1, high-mobility group box 1 protein; TIM-3, T-cell immunoglobulin- and mucin-domain-containing molecule; TLR, toll-like receptor

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treated with chemoradiotherapy, it is well known that the clinical benets vary considerably, with some good and some poorly responsive patients. For poor responders, the therapeutic benets are often dubious and side effects can be considerable. If the precise mechanisms underlying immunogenic cell death induced by chemoradiotherapy were dened, there would potentially be two clinical applications. One would be prognostic, that is, to foretell the extent of benet of chemoradiotherapy by measuring surrogate markers such as HMGB1, calreticulin, TIM-3 or galectin-9. The other application would be for treatment, that is, to enhance the therapeutic benet of the chemoradiotherapy by sequentially combining it with cancer vaccine and other immune-activating therapies in patients with the biomarker prole of potential good responders. As the cancer-testis antigens have been identied and used for clinical trials in a variety of cancer, it is highly desirable to develop the therapeutic combination of chemoradiotherapy with cancer vaccine using the cancer-testis antigens.

Conclusion and Perspectives

At present, immunogenic tumor cell death is not explained merely by the HMGB1/TLR4-dependent pathway alone, and clinical outcome after the treatment is not predicted by serum levels of HMGB1.68,69 However, a better understanding of the molecular and cellular mechanisms underlying the chemoradiotherapy-induced immunogenic tumor cell death may help develop critical algorithms for the management of cancer patients. For example, we will be able to categorize the anticancer treatments that do or do not mediate an immunogenic cell-death pathway. Alternatively, we will be able to predict an optimal response to chemoradiotherapy based on danger signal-related molecules. Finally, we will be able to select the best adjuvants synergizing with immunogenic chemoradiotherapy. A novel approach based on immunogenic cell-death concept is presently being investigated in prospective clinical trials in prostate cancer and melanoma, testing a combination of local irradiation with ipilimimab.

Furthermore, it is desirable to establish crucial mice models in order to elucidate molecular and cellular mechanisms underlying the immunogenic tumor cell death and evaluate a signicance of immunotherapy.

Conict of InterestThe authors declare no conict of interest.

Acknowledgements. We are grateful to Dr. Aniruddha Choudhury for critical reading of the article.

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