Corrigendum: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan

DOI: 10.1038/ncomms11530 OPEN

Corrigendum: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan

Markus Schosserer, Nadege Minois, Tina B. Angerer, Manuela Amring, Hanna Dellago, Eva Harreither, Alfonso Calle-Perez, Andreas Pircher, Matthias Peter Gerstl, Sigrid Pfeifenberger, Clemens Brandl,Markus Sonntagbauer, Albert Kriegner, Angela Linder, Andreas Weinhausel, Thomas Mohr, Matthias Steiger, Diethard Mattanovich, Mark Rinnerthaler, Thomas Karl, Sunny Sharma, Karl-Dieter Entian, Martin Kos, Michael Breitenbach, Iain B.H. Wilson, Norbert Polacek, Regina Grillari-Voglauer, Lore Breitenbach-Koller& Johannes Grillari

Nature Communications 6:6158 doi: http://dx.doi.org/10.1038/ncomms7158

Web End =10.1038/ncomms7158 (2015); Published 30 Jan 2015; Updated 11 May 2016

This Article contains an error in the numbering of the C. elegans 25S rRNA site methylated by nsun-5, which was incorrectly given as C3381. The correct methylation site is C2381. The correct version of Fig. 4 and its legend is depicted below. In addition, in the Discussion section of this Article, the sentence By applying the same method to C. elegans, we could prove that C3381, which corresponds to yeast C2278, is indeed methylated by nsun-5 (Fig. 4e) should read By applying the same method to C. elegans, we could prove that C2381, which corresponds to yeast C2278, is indeed methylated by nsun-5 (Fig. 4e).

NATURE COMMUNICATIONS | 7:11530 | DOI: 10.1038/ncomms11530 | http://www.nature.com/naturecommunications

Web End =www.nature.com/naturecommunications 1

CORRIGENDUM NATURE COMMUNICATIONS | DOI: 10.1038/ncomms11530

a

Rcm1:

C330

C404

1 156 483 490

SAM-binding (246332)

N-terminal domain

RNA-methyltransferase region C-terminus

tRNAs

b

Small subunit Large subunit

Decoding

center

mRNA

Peptidyl-transferase center

c

S. cerevisiaeD. melanogasterC. elegans

2250

3592 2353

2301 3643 2404

d

e

f

Polysomes Monosomes

1.25 ** ****

100

*

*

*

*

*

C2278 methylation [%]

80 60 40 20

0

C2381 methylation [%]

100

80 60 40 20

0

*

1.00

Fraction alive [%]

0.75

*

0.50

0.25

0.00 0 5 10 15 20 25

0 min 30 min 90 min 0 min

30 min 90 min

H2O2 stress

Time [d]

Wt rcm1

N2 nsun-5

rDNA, rDNA (wt) rDNA, rDNA (C2278G)

Figure 4 | The conserved m5C-rRNA methyltransferase activity of Rcm1 mediates lifespan extension. (a) Rcm1 is a protein of 490 amino acids harbouring an RNA methyltransferase domain with two highly conserved cysteines, C330 and C404, which are predicted to participate in the catalysis of methyl transfer. (b) Model of the yeast ribosome with functional elements. The large and small ribosomal subunits, RPs of the SSU in blue and of the LSU in red, are shown assembled on the mRNA, the tRNAs in the aminoacyl site (A, pink), in the peptidyl site (P, magenta) and in the exit site (E, cyan) are depicted, and cytosine 2278 (C2278) of the 25S rRNA in vicinity of the peptidyltransferase center is highlighted in orange. (c) 25S rRNA sequence tract, nucleotide 22512300, harbours a series of highly conserved modied nucleotides, pseudouridinylations in green and guanine and adenine base methylations in red and the single m5C-methylation in orange. This region is 100% conserved from S. cerevisiae to C. elegans and D. melanogaster. (d,e) Bisulte sequencing of wt yeast cells detects C2278 methylation in rRNA isolated from ribosomal fractions independent of oxidative stress. Deletion of rcm1 resulted in a complete lack of cysteine C2278 methylation under all conditions tested (d) Bisulte sequencing of N2 wt C. elegans conrms conservation of C2381 m5C-methylation, while deletion of nsun-5 resulted in a complete lack of this modication (e). Displayed values represent fraction of C2278/C2381 methylation minus average fraction of unconverted cytosines except C2278/C2381 as unspecic background. Grubbs Test at a 0.01 was performed on all cytosines in the sample and asterisk *

marks samples with C2278/C2381 identied as signicant outlier. (f) Deletion of ribosomal DNA in haploid S. cerevisiae and rescue with unmethylated rDNA (C2278G) on a plasmid increases chronological lifespan in SC compared with wt rDNA (wt). Error bars represent s.e.m. of three biological replicates (multiple comparison adjusted two-way ANOVA with Sidak post test, a 0.05, **Po0.01, ****Po0.0001).

2 NATURE COMMUNICATIONS | 7:11530 | DOI: 10.1038/ncomms11530 | http://www.nature.com/naturecommunications

Web End =www.nature.com/naturecommunications

NATURE COMMUNICATIONS | DOI: 10.1038/ncomms11530 CORRIGENDUM

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Web End =http://creativecommons.org/licenses/by/4.0/

NATURE COMMUNICATIONS | 7:11530 | DOI: 10.1038/ncomms11530 | http://www.nature.com/naturecommunications

Web End =www.nature.com/naturecommunications 3