Identification of proliferative progenitors associated with prominent postnatal growth of the pons

Abstract

The pons controls crucial sensorimotor and autonomic functions. In humans, it grows sixfold postnatally and is a site of paediatric gliomas; however, the mechanisms of pontine growth remain poorly understood. We show that the murine pons quadruples in volume postnatally; growth is fastest during postnatal days 0-4 (P0-P4), preceding most myelination. We identify three postnatal proliferative compartments: ventricular, midline and parenchymal. We find no evidence of postnatal neurogenesis in the pons, but each progenitor compartment produces new astroglia and oligodendroglia; the latter expand 10- to 18-fold postnatally, and are derived mostly from the parenchyma. Nearly all parenchymal progenitors at P4 are Sox2⁺ Olig2⁺ , but by P8 a Sox2^- subpopulation emerges, suggesting a lineage progression from Sox2⁺ 'early' to Sox2^- 'late' oligodendrocyte progenitor. Fate mapping reveals that >90% of adult oligodendrocytes derive from P2-P3 Sox2⁺ progenitors. These results demonstrate the importance of postnatal Sox2⁺ Olig2⁺ progenitors in pontine growth and oligodendrogenesis.

ARTICLE

Received 20 Mar 2015 | Accepted 14 Apr 2016 | Published 18 May 2016

Robert A. Lindquist1,2,3, Cristina D. Guinto1,4, Jose L. Rodas-Rodriguez1,4, Luis C. Fuentealba1,4, Matthew C. Tate1,4, David H. Rowitch1,5 & Arturo Alvarez-Buylla1,4

The pons controls crucial sensorimotor and autonomic functions. In humans, it grows sixfold postnatally and is a site of paediatric gliomas; however, the mechanisms of pontine growth remain poorly understood. We show that the murine pons quadruples in volume postnatally; growth is fastest during postnatal days 04 (P0P4), preceding most myelination. We identify three postnatal proliferative compartments: ventricular, midline and parenchymal. We nd no evidence of postnatal neurogenesis in the pons, but each progenitor compartment produces new astroglia and oligodendroglia; the latter expand 10- to 18-fold postnatally, and are derived mostly from the parenchyma. Nearly all parenchymal progenitors at P4 are Sox2 Olig2, but by P8 a Sox2 subpopulation emerges, suggesting a lineage progression from Sox2 early to Sox2 late oligodendrocyte progenitor. Fate mapping reveals that 490% of adult oligodendrocytes derive from P2P3 Sox2 progenitors. These results demonstrate the importance of postnatal Sox2 Olig2 progenitors in pontine growth and oligodendrogenesis.

DOI: 10.1038/ncomms11628 OPEN

Identication of proliferative progenitors associated with prominent postnatal growth of the pons

1 Eli & Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of CaliforniaSan Francisco, San Francisco, California 94143, USA.

2 Medical Scientist Training Program, University of CaliforniaSan Francisco, San Francisco, California 94143, USA. 3 Neuroscience Graduate Program, University of CaliforniaSan Francisco, San Francisco, California 94143, USA. 4 Department of Neurological Surgery, University of CaliforniaSan Francisco, San Francisco, California 94143, USA. 5 Department of Pediatrics, University of CaliforniaSan Francisco, San Francisco, California 94143, USA. Correspondence and requests for materials should be addressed to A.A.-B. (email: mailto:[email protected]

Web End [email protected] ).

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The mammalian brain undergoes signicant postnatal growth. Postnatal growth could aid experience-dependent acquisition of brain function1, and may confer

susceptibility to tumorigenesis2. The human pons is a site of exceptional postnatal growth3 and lethal paediatric gliomas4; however, knowledge of the cells responsible for pons growth remains limited. One study suggested a second peak of Nestin progenitor cells in ventral human pons at 7 years5, though a subsequent study did not nd evidence to support that claim, and instead observed that such progenitors were restricted to infancy3. The nature of postnatal pontine progenitor cells has consequences for pontine gliomagenesis, and for the normal postnatal development and function of this crucial brain region.

The dorsal pons (tegmentum) contains autonomic nuclei controlling awakeness and arousal6, respiration7 and adrenergic tone8, as well as cranial nerves and nuclei mediating facial sensation and motion9. The ventral pons (basis pontis) contributes to motor function, in part as a bridge between cortex and cerebellum: neurons in the pontine grey nuclei receive synapses from cortex and project to cerebellum via the middle cerebellar peduncle10. Interestingly, among primates the size of the basis pontis scales directly with the size of the neocortex11. The basis pontis also contains the corticospinal tract, whose descending axons carry motor signals from cortex to spinal motor neurons. Many pontine circuits serve functions that are postnatally acquired or rened, but nearly all studies of pons development have focused on prenatal events, beginning with its embryonic derivation from various segments of the rhombencephalon. Pontine neurogenesis occurs prenatally1217: autonomic nuclei and cranial nerves (VVIII) derive mainly from rhombomeres 2 to 5 (refs 18,19), though some tegmental neurons derive from rhombomere 1 (ref. 20); the neurons of the basis pontis are born in rhombomeres 68 and migrate anteriorly to form the pontine grey nuclei in the territory of rhombomeres 34 (refs 20,21). Proliferation22 and gliogenesis2326 have been documented in embryonic murine and avian pons, but the question of which cells are responsible for postnatal pontine growth remains unanswered.

Here we show that as in humans, the postnatal mouse basis pontis grows more than tegmentum, with growth comparable to rapidly growing forebrain structures; growth is fastest at birth and mostly occurs before P16. Postnatal progenitor cells in the pons are found (1) in the ventricular zone along the ventral walls of the fourth ventricle, (2) in the midline domain and (3) in the parenchyma. Progenitors proliferate in a single wave peaking at postnatal day 4. We observe that proliferative Olig2 progenitors include an unexpected and abundant Sox2 subpopulation, which is enriched in basis pontis. Remarkably, postnatal Sox2 progenitor cells produce more than 90% of adult mouse pons oligodendrocytes, contributing to a 10- to 18-fold postnatal expansion of the oligodendroglial lineage.

ResultsPostnatal pontine growth rates are fastest from P0 to P4. The human pons grows sixfold from birth to adulthood3. To measure the postnatal growth of the mouse pons, we prepared CD1 wild-type mouse tissue for sectioning in a plane containing basis pontis, tegmentum and fourth ventricle (Fig. 1a and Methods section). We computed volumes of basis pontis and tegmentum based on areas measured in every second section through the pons (Fig. 1bd). The basis pontis grew vefold postnatally, from0.38 mm3 at birth to 1.96 mm3 in adulthood (Fig. 1c), while the pontine tegmentum grew fourfold, from 2.48 mm3 to 10.3 mm3 (Fig. 1d). This growth was comparable to the four- to vefold postnatal increase in mouse hippocampal and neocortical volume

measured by magnetic resonance imaging27 and histologic methods28, and greater than the two- to threefold postnatal increase in volume of anterior thalamic nuclei28. The absolute volumetric growth rate (mm3 per day) was greater in tegmentum than in basis pontis, due to the tegmentums larger size, and in tegmentum the absolute rate of growth was greatest during P4P10 (Fig. 1e). However, the daily per cent increase in volume was greatest during P0P4, and was greater in basis pontis than tegmentum during that period, with basis pontis growing at an average rate of 16% daily (Fig. 1f). The rate of growth then declined gradually through weaning age. The size of the pons at P24 was not signicantly different from that in adults aged P64 (P40.2, unpaired t-test). These data indicate that the mouse pons grows rapidly during early postnatal life and proportionally more in basis pontis than tegmentum.

Myelination does not account for pontine growth from P0 to P4. The rapid early postnatal growth of human basis pontis is attributable in part to myelination3. To determine whether myelination accounted for the rapid early growth of the postnatal mouse basis pontis, we performed immunohistochemistry for myelin basic protein (MBP). The distribution of MBP in pontine sections of increasing age is shown in Fig. 2ac. MBP appeared in concert with adenomatous polyposis coli (APC), a marker of mature oligodendrocytes, rst in selected white matter tracts of tegmentum, for example, medial longitudinal fasciculus and trigeminal nerve (V) by P2P4, followed by white matter of basis pontis from P8 to P10 and grey matter of basis pontis and tegmentum by P8 to P12 (Fig. 2d). The extent of myelination and the apparent thickness of myelinated tracts increased throughout the pons between P10 and P24 (Fig. 2c,d). The progression of myelination evidenced by MBP staining is consistent with a magnetic resonance imaging study on the development of 12 selected white matter tracts in postnatal mouse brain (including three brainstem tracts)27. Interestingly, while our data show that the pons underwent its most rapid growth before P4, hardly any MBP bres were observed in P4 basis pontis (Fig. 2b,d). This suggests that, in contrast to human, the mouse pons shows rapid early postnatal growth before extensive addition of myelin.

Proliferation occurs in three germinal zones and peaks at P4. On the basis of the ndings above, we hypothesized that early postnatal pontine growth could be due to proliferation of neuronal and/or glial precursor cells. To measure proliferation in postnatal development, we injected an age series of CD1 wild-type mice with a single dose of the thymidine analogue BrdU, 100 min before perfusion (Fig. 3a). Immunostaining revealed BrdU cells throughout the mouse brainstem (Fig. 3b,c); the density of BrdU cells was greater in basis pontis than in any other region of the brainstem (Fig. 3d,h; Supplementary Fig. 3f,g), and also greater in the pontine tegmentum relative to midbrain tegmentum (Supplementary Fig. 3h). We observed three main anatomical compartments of BrdU cells: (1) along the ventral surface of the fourth ventricle (ventricular zone, VZ); (2) along the midline; and (3) within parenchyma (Fig. 3e). In all three compartments, the density of BrdU cells was greatest at P4 (Fig. 3fh), coinciding with the period of fastest pons growth (Fig. 1e,f). Notably, in the parenchymal compartment, BrdU cells were denser in the basis pontis than in the tegmentum (Fig. 3h). This greater proliferation in basis pontis coincides with its larger daily per cent increase in volume (Fig. 1f). Basis pontis proliferation peaked at 27615 cells per mm2 at P4, and at P12 was still more than half its P4 peak, while tegmentum proliferation peaked at 19639 cells per mm2 at P4, and declined more than 50% by P12 (Fig. 3h). Similarly, proliferation in VZ and midline peaked at P4 with 40.63.1 and 16.33.7 cells per

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Figure 1 | Postnatal growth of mouse pons. (a) DAPI stain of P0 mouse pons section containing tegmentum (cyan) and basis (magenta), sectioned at oblique angle normal to axis of spinal cord (inset). Basis pontis includes pontine nuclei and adjacent white matter tracts (transversus fasciculus pontis, longitudinalis fasciculus pontis, and middle cerebral peduncle ventral to pontine nuclei). Scale bar 1 mm. (b) Postnatal growth of pons in dorsal-ventral

and medial-lateral axes is illustrated by section tracings as in (a); growth along rostral-caudal axis is indicated by the number of sections passing through pons. Scale bar 1 mm. (c,d) Volumes of pons regions were computed by summation of areas measured in every second section through pons, times

distance between measured sections (100 mm). Graphs show individual replicates with means.e.m., nZ3 mice per timepoint. Unpaired t-test revealed signicant differences between the following consecutive timepoints: P0 and P4 (both regions), P4 and P10 (both regions), and P10 and P16 (tegmentum).

*Po0.05, **Po0.01, ***Po0.001. (e) Absolute increase in volume per day computed as (mean volume at end of intervalmean volume at beginning of interval) (number of days in interval). (f) Per cent increase in volume per day computed as (100 ((mean volume at end of interval mean volume at

beginning of interval)(1/number of days in interval) 1)).

mm, respectively, and declined more than 50% by P8 and P12, respectively (Fig. 3f,g). There was a signicant increase in proliferation density from P0 to P4 in VZ (P 0.0302, unpaired

t-test) and basis pontis (P 0.0072), while in tegmentum and

midline the increased density of BrdU cells from P0 to P4 was

not statistically signicant (P 0.128 and P 0.121, respectively).

Parenchymal proliferation declined by P20 to o10% of its P4 peak, and in adulthood (P64) amounted to 1.5% of its P4 peak (Fig. 3h). VZ proliferation declined sooner, reaching o10% of its

P4 peak by P12 and 1.5% of its P4 peak by P20 (Fig. 3f). We observed no BrdU VZ cells at P28 or above. These ndings show that the pons is the most proliferative postnatal brainstem region; its cell proliferation shows a single postnatal peak, at P4, coincident with the period of fastest growth. These data also show that proliferation declines at different rates in pontine VZ, parenchyma, and midline, and at different levels of the brainstem.

Neurogenesis does not occur in the postnatal pons. The birthdating studies of Altman and Bayer1217 indicate that neurons of the rat pons are primarily born before birth.

However, it is not known if some pontine neurons are born postnatally in mouse. Given the dramatic postnatal growth (Fig. 1) and the presence of some migrating precerebellar neurons entering the pontine grey nuclei as late as P0 (ref. 29), it was possible that some pontine neurons could be born postnatally. We delivered BrdU in a single dose to wild-type mice aged P0 or P4. Analysis at P28 revealed no BrdU NeuN cells in either basis pontis or tegmentum; since this BrdU dosage regimen might have been insufcient to identify slowly or rarely dividing cells, we administered BrdU or EdU every 12 h over a 4-day course spanning ages P1P4, P5P8 (Supplementary Fig. 4a), P9P12 and so on through P29P32, followed by 28-day chase periods. (The use of EdU in fate mapping may have reduced survival of EdU-labelled cells on a second cell cycle post labelling30,31, but would not have affected cells undergoing a single cell cycle.) Many cells retained BrdU and/or EdU after 28-day chase, including some glial brillary acidic protein (GFAP) astrocytes and APC oligodendrocytes (Supplementary Fig. 4b,c); yet we observed no EdU NeuN or BrdU NeuN cells in the pons (Supplementary Fig. 4d). Finally, we performed co-staining of the

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Figure 2 | Progressive myelination in pons. (a) P16 pons section stained for myelin basic protein (MBP) was traced to highlight landmark tracts and grey/white regions. Lines indicate thick bres within grey matter regions. (b,c) MBP-stained pons hemisections at indicated ages; red, MBP; blue, DAPI. Scale bar for (ac) 1 mm. (d) High-resolution images of representative pons regions, including both grey and white matter within tegmentum and

basis, illustrate differently timed regional onsets of myelination and progressive increase over postnatal development. Red, MBP; green, APC; blue, Olig2. Scale bar, 50 mm. LFP, longitudinal fasciculus pontis; MCP, middle cerebellar peduncle; ML, medial lemniscus; MLF, medial longitudinal fasciculus; PCG, pontine central grey; PN, pontine nuclei; TFP, transverse fasciculus pontis.

neuroblast markers doublecortin (DCX) and PSA-NCAM with BrdU in mice aged P0, P4, P8y through P32, each perfused 100 min after a single dose of BrdU, and found no colocalization of BrdU with either DCX or PSA-NCAM (Supplementary Fig. 4e,f). Altogether, these results indicate that pontine neurons are not born postnatally in mice, and thus neurogenesis does not account for postnatal pontine growth.

Postnatal expansion of the oligodendroglial lineage. Because the pons lacks postnatal neurogenesis and undergoes delayed but extensive myelination, we hypothesized that pons growth may coincide with an increase in oligodendroglia. To count the oligodendroglial population in the developing postnatal pons, we performed stereologic analysis of wild-type tissue sections co-stained for Sox10, Olig2 and APC (Fig. 4af). Oligodendrocyte precursor cells (OLPs) were dened as Sox10 Olig2APC ;

oligodendrocytes (OLs) were dened as APC cells coexpressing Sox10, Olig2 or both (Fig. 4a). At birth, the basis pontis contained 6,357610 OLPs, increasing to 13,688790 OLPs by P4 and 29,527922 by P10; OLPs peaked at P16 at 35,3695,808, and declined by P24 to 10,3722,004 (Fig. 4d). The number of mature OLs in basis pontis increased markedly from 958212 at P4 to 26,1192,666 at P10 and 88,75011,417 at P16, after which the number of mature OLs in basis pontis remained constant to adulthood (Fig. 4d). The tegmentum underwent similar phases: the number of OLs in tegmentum was 19,9902,612 at P4, increasing to 214,99649,232 by P10 and 443,48035,041 by P16 (Fig. 4e). The density of OLPs and morphology of APC staining changed markedly through post-natal development (Fig. 4c), reecting the progressive increase and maturation of oligodendroglia through three phases: OLP expansion without differentiation (P0P4), OLP expansion and

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d BrdU+ cell density in ventral brainstem

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Figure 3 | BrdU reveals three compartments of pontine proliferation. (a) CD1 wild-type mice were given a single dose of BrdU by intraperitoneal injection (50 mg kg 1) 100 min before perfusion, thereby labelling acutely proliferative cells. Standard coronal sections indicate levels of basis pontis (b) and pontine 4th VZ (c). (b,c) DAPI and BrdU costains at levels shown in (a) reveal proliferation throughout the mouse brainstem, but concentrated in basis pontis (b) and along 4th ventricle (c). Scale bar, 1 mm. (d) Density of BrdU cells is greater in basis pontis (ventral pons) than in ventral midbrain (F1,32 78.16,

Po0.0006) or ventral medulla (F1,32 11.53, P 0.0108; regional comparisons by two-way ANOVA and Bonferroni post hoc adjustment; graph shows

means.e.m., n 3 mice per timepoint). See Supplementary Fig. 3 for dorsal brainstem regions. (e) Representative sections at level of (c) traced in

StereoInvestigator software. Each mark represents one BrdU nucleus. Cyan marks represent BrdU cells in VZ, which was dened by the density of DAPI along the 4th ventricle; midline populations were dened by contact with vimentin (dark blue) or GFAP (green) bres that contacted the midline glial structure; all other BrdU nuclei were considered parenchymal and labelled red. Scale bar, 1 mm. (f) Density of proliferative cells along pontine VZ; graph shows individual replicates with means.e.m. (n 3 mice per timepoint); comparisons are by unpaired t-test. (g) Density of BrdU cells along midline;

graph shows individual replicate values with means.e.m. (n 3 mice per timepoint). (h) Density of BrdU cells is greater in basis pontis than pontine

tegmentum (F1,40 39.06, Po0.0006; regional comparisons by two-way ANOVA and Bonferroni post hoc adjustment; graph shows means.e.m., n 3

mice per timepoint). *Po0.05; **Po0.01, ***Po0.001. NS, not signicant.

differentiation to OLs (P4P16), and OLP decline (P16P24). Between P0 and P16, the oligo-lineage population increased 18-fold in basis pontis and 10-fold in tegmentum (Fig. 4f). The stereologic count of oligodendroglia, therefore, revealed a marked postnatal increase in oligodendroglia, and raised the question of which progenitor domains give rise to these new cells.

Postnatal VZ and midline domains add few cells to parenchyma. Co-immunostaining for BrdU and intermediate laments revealed proliferative progenitors associated with GFAP and

Vimentin bres in the postnatal VZ and midline domains (Figs 5a and 6a,b). Both VZ and midline also contained Nestin cells at P4 (Supplementary Fig. 5), and a subpopulation of VZ

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Figure 4 | Expansion and maturation of OL lineage in postnatal pons. CD1 wild-type mouse pons sections were stained for Sox10, Olig2 and APC. (a) Representative OLP (Olig2Sox10 APC , magenta arrow) and OL (Olig2 Sox10 APC, white arrow) from P10 mouse basis pontis. Scale bar, 5 mm. (b) Schematic of basis pontis region shown at several ages in (c). Scale bar, 1 mm. (c) Age series from basis pontis shows postnatal changesin density and maturation state of OLPs and OLs. Note sequential upregulation, condensation, and diminution of APC over time. Scale bar, 50 mm.

(d,e) Stereologic counts of pons oligodendroglia in basis pontis (d) and tegmentum (e) over postnatal development, measured by Optical Fractionator probe in StereoInvestigator software. Cells were identied as OLs if they expressed APC and either or both of Sox10/Olig2; cells were counted as OLPs if they expressed both Sox10 and Olig2 but not APC. (f) Counts of OLPs (purple) and total oligodendroglia (OLs OLPs, black) were normalized to P0

values, revealing fold expansion of the oligodendroglial population since P0 (1 ). All graphs show individual replicates with means.e.m. of n 3 mice per

timepoint.

progenitors at M-phase showed phospho-Ser55-Vimentin staining in cells with radial glial-like morphology (Fig. 5b). The presence of proliferative cells and progenitor markers led us to ask whether these domains contribute new cells, and particularly oligodendroglia, to the growing pons parenchyma. We performed viral fate mapping of VZ progenitors by delivering adenoviral Cre into the lateral ventricles of P1 Ai14 or Ai14;ALDH1L1:GFP mice32,33, thereby labelling VZ cells throughout the entire ventricular system (including fourth ventricle) with the TdTomato red uorescent protein (Fig. 5ce), and labelling also a small number of tegmental ALDH1L1:GFP astrocytes and

Sox10 oligodendroglia (Fig. 5ei). We performed a similar labelling of mitotic, Nestin VZ progenitors by delivering

RCAS:GFP retrovirus to the lateral ventricles of P2 mice carrying the Nestin-tva allele34, and again observed a small

number of tegmental oligodendroglia close to the fourth ventricle (Supplementary Fig. 6). To label the midline domain, we used the FoxA2CreER driver35 crossed to the Ai14 reporter, and observed TdTomato expression in midline glial bres as well as a small subpopulation of nearby astroglia and oligodendroglia (Fig. 6cg). Altogether, these experiments showed that while both VZ and midline domains produced both oligodendroglia and astroglia, their parenchymal progeny were few in number and remained close to VZ or midline.

OLPs are the most proliferative population in postnatal pons. We turned our attention to the parenchymal progenitors, the largest progenitor pool in the postnatal pons. Again using BrdU to label proliferative cells 100 min before perfusion, we used mice carrying cell type-specic reporter transgenes to determine

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Figure 5 | Properties and progeny of postnatal fourth VZ progenitor cells. (a) Wild-type mice received BrdU in a single dose 100 min before perfusion. BrdU nuclei in postnatal 4th VZ are surrounded by bres of GFAP and vimentin. Scale bar, 20 mm. (b) Co-staining for M-phase marker phospho-histone-

H3 (pH3) and phospho-ser55-Vimentin (p-Vimentin) reveals radial glial-like morphology of 4th VZ progenitors. Scale bar, 20 mm. (ci) Viral fate mapping of postnatal 4th VZ progenitors. Ai14 or Ai14;ALDH1L1:GFP pups received Adeno-Cre viral injection in lateral ventricle at age P1, and were perfused at P4 (100 min after a single dose of EdU) or at P24. (d) Co-staining of P4 tissue sections for EdU, GFAP, and TdTomato reveals proliferative TdT nuclei in the

VZ of the 4th ventricle. Scale bar, 20 mm. (e) Cre-recombined cells are visible in olfactory bulb, conrming successful targeting of forebrain V-SVZ progenitors; along the surface of the entire ventricular system, including pontine 4th ventricle; and along the ventral surface of the brain, suggesting viral outow to meningeal space via cisterna magna. Scale bar, 1 mm. (f) Enlarged view of 4th VZ and pontine tegmentum region indicated in (e), revealing parenchymal TdTomato (TdT) cells. Scale bar, 100 mm. (g) Further enlargment of pontine tegmentum as indicated in (f), showing co-immunostaining for

TdTomato (Red) and lineage-specic markers (Green: left, Sox10; right, ALDH1L1:GFP). Scale bar, 50 mm. (h) Enlarged view of basis pontis region indicated in (e), revealing parenchymal TdT cells. Scale bar, 100 mm. (i) Further enlargment of basis pontis as indicated in h, showing immunostaining for TdT and lack of colocalization with either Sox10 (left) or ALDH1L1:GFP (right). Many TdT cells are found along structures morphologically consistent with blood vessels. Scale bar, 50 mm.

the identity of proliferative cells (Fig. 7a). NG2:DsRed mice report DsRed in NG2-expressing cells, including OLPs and pericytes36, so we co-stained for Sox10 and identied OLPs as BrdU DsRed Sox10 triple-positive cells (Fig. 7b).

ALDH1L1:GFP mice report GFP in astrocytes32, and proliferative parenchymal astrocytes were vimentin as well (Fig. 7c). It was difcult to colocalize nuclear and lamentous markers, so we

identied proliferative astrocytes as BrdU ALDH1L1:GFP cells. Co-staining for Olig2 generally revealed segregation from astrocytes (Fig. 8g, Supplementary Fig. 7b); however, a small subpopulation of BrdU ALDH1L1:GFP Olig2 astrocytes was also identied (Fig. 8h, Supplementary Fig. 7c). We measured proliferation among these cell types through postnatal development and found that proliferative OLPs outnumbered

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Figure 6 | Proliferative progenitors in postnatal pons deriving from midline domain. (a) Wild-type mouse pons sectioned at oblique angle and stained for vimentin (green) and DAPI (magenta). Note the intense vimentin staining along the midline. Scale bar 500 mm. (b) Pons tissue from a mouse

perfused 100 min after a single dose of BrdU (50 mg kg 1) was co-stained for Vimentin, GFAP and BrdU, revealing proliferating cells among bres of the midline. Scale bar, 20 mm. (c) Schematic of fate mapping of midline domain. (d) TdTomato labelling by FoxA2CreER;Ai14 in caudal pons, extending from fourth ventricle along midline, and including nearby cells. The midline and nearby cells were clearly labelled, along with other pontine structures known to derive from FoxA2 progenitors (ref. 67): the motor nuclei of cranial nerves V, VI and VII, and raphe nuclei. Scale bar, 500 mm. (e) TdTomato expression in bres along midline and in nearby parenchyma of basis pontis. Scale bar, 100 mm. (f) Co-labelling of TdTomato, EdU, and Sox10 reveals P4 proliferative oligodendroglia in tegmentum labelled by FoxA2CreER recombination at E9.5. Magenta arrows indicate Sox10 EdU TdT cells. Scale bar, 50 mm.

(g) Co-labelling of TdTomato, EdU, and ALDH1L1:GFP reveals that some P4 proliferative astrocytes are labelled by FoxA2CreER recombination at E9.5. Yellow arrows indicate ALDH1L1:GFP EdU TdT cells; magenta arrows indicate GFP EdU TdT cells. Scale bar, 50 mm. (dg) In a section of bilateral width 4 mm, we observed no TdT EdU cells further than 500 mm from the midline; the only TdT cell bodies beyond 1 mm from midline were trigeminal motor neurons. These results show that while the midline domain produces postnatally proliferative astroglia and oligodendroglia, these progeny are regionally restricted.

proliferative astrocytes at all ages from P4 onward (Fig. 7d). In P4 basis pontis, the densities of these cell types were 192.82.7 proliferative OLPs per mm2 and 73.03.9 proliferative astrocytes per mm2; in P4 tegmentum, 151.410.3 proliferative OLPs per mm2 and 12.42.9 proliferative astrocytes per mm2. Proliferation persisted to a later age in basis pontis than tegmentum for both cell types: proliferative OLPs in basis pontis showed a sustained peak density from P4 to P8, while OLP proliferation in tegmentum peaked at P4 and began to decline by P8; proliferative astrocytes plateaued from P0P4 in basis pontis, but declined in tegmentum as soon as P4. OLP proliferation declined to o10% of its peak in both basis pontis and tegmentum by P24, while astrocyte proliferation decreased to o10% of its peak by P8 in tegmentum, and P12 in basis pontis. In adults (P64), we observed a small number of proliferative OLPs in both basis pontis (2.410.74 cells per mm2) and tegmentum

(3.151.28 cells per mm2), comparable to the total proliferation in adult wild-type pons parenchyma (Fig. 3h); no astrocyte proliferation was observed after P12.

The predominance of OLPs at the P4P8 proliferative peak, and the marked postnatal increase in OL number, raised the question of what proportion of OLPs are actively cycling. We therefore administered BrdU repeatedly to wild-type mice from P4.5P8, and perfused mice 100 min after the last dose, at P8. We observed BrdU in 86.0%2.6% of OLPs in basis pontis, and63.2%4.2% of OLPs in tegmentum (Supplementary Fig. 4gi). This result echoes the denser OLP proliferation in basis pontis than tegmentum (Fig. 7d), suggests that nearly all basis pontis OLPs divided during the postnatal proliferative peak between P4P8, and may in part account for the greater expansion of the oligodendroglial population in basis pontis compared with tegmentum (Fig. 4f).

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Figure 7 | Glial lineages of parenchymal proliferative progenitors. (ad) Mice carrying lineage-specic reporter transgenes received a single dose of BrdU (50 mg kg 1) 100 min before perfusion. (b) Representative proliferative OLP (Sox10 DsRed BrdU) in P4 NG2:DsRed mouse basis pontis. Scale bar, 10 mm. (c) Representative proliferative parenchymal astrocyte (BrdU GFP ) in P4 ALDH1L1:GFP mouse basis pontis, with vimentin processes suggestive of colocalization. Scale bar, 10 mm. (d) Densities of proliferative OLPs from NG2:DsRed mice (red), and proliferative parenchymal astrocytes from ALDH1L1:GFP mice (green), at ages through childhood and at adulthood; means.e.m., n 3 mice per strain per timepoint. Three-factor ANOVA conrmed

signicant differences in proliferation based on cell type (F1,56 491.97, Po0.0001), region (F1,56 70.18, Po0.0001), and age (F9,56 97.97, Po0.0001).

(eh) Wild-type or ALDH1L1:GFP mice were given a single dose of BrdU (50 mg kg 1) at P4 and perfused at P28. (f) BrdU cells from basis pontis of mice prepared as in e. Top, representative mature oligodendrocyte (Olig2APC BrdU ); bottom, representative OLP (Olig2APC BrdU ). Scale bar, 10 mm.

(g) A representative GFAP ALDH1L1:GFP BrdU astrocyte from basis pontis of an ALDH1L1:GFP mouse that was given BrdU at P4 and perfused at P28. Scale bar, 10 mm. (h) Quantitation of BrdU astrocytes and oligodendroglia detected in pons regions after pulse at P4 and chase to P28; graph shows individual replicates with means.e.m., n 3 mice per strain. Unpaired t-test shows BrdU astrocytes outnumber BrdU oligodendroglia in basis pontis

(P 0.0099) and are in equal quantity to BrdU oligodendroglia in tegmentum (P 0.4320), while oligodendroglia are a lower fraction of BrdU cells in

P28 basis pontis than tegmentum (See text for interpretations, P 0.0369). *Po0.05; **Po0.01; NS P40.05. NS, not signicant.

Despite the nding that proliferative OLPs outnumber proliferative astrocytes, a pulse-chase experiment (Fig. 7eh) with a single dose of BrdU at P4 yielded, among BrdU cells at

P28, more astrocytes (60.7%5.3%) than oligodendroglia(28.6%4.0%) in basis pontis, and an even ratio of astrocytes to oligodendroglia in tegmentum (49.1%4.7% versus44.3%2.4%). Taken together with our stereologic counts of oligodendroglia (Fig. 4), this result suggested two possibilities:(1) P4 OLPs may divide so many times by P28 that many of them dilute out their BrdU signal, and/or (2) oligodendroglia might undergo cell death. Consistent with the rst possibility, BrdU staining was weaker and more punctate in P28 OLs than in P28 astrocytes (Fig. 7f,g). Further supporting the rst possibility, oligodendroglia accounted for a lower per cent of P28 BrdU cells in basis pontis than in tegmentum (Fig. 7h). Supporting the

second possibility, apoptosis has been documented in young OLs37, and in P12 basis pontis we observed Sox10 nuclei surrounded by cytoplasmic, cleaved caspase-3 staining in an

OL-like morphology (Supplementary Fig. 8e). We also investigated a third possibility, that P4 OLPs might produce astrocytes, by using NG2CreER mice38. NG2CreER;Ai14; ALDH1L1:GFP mice receiving tamoxifen at P0 or P4 did not show any TdTomato (TdT ) cells expressing ALDH1L1:GFP at P8, or any TdT cells expressing GFAP at P60, but instead showed TdT pericytes and Sox10 or Olig2 APC cells (Supplementary Fig. 8ad), consistent with forebrain38.

Altogether, these experiments show that the postnatal pons has proliferative OLPs and astrocytes, pons OLPs are lineage restricted, and OLPs are the predominant proliferative population at all postnatal ages.

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Figure 8 | Sox2 is expressed in most postnatal pons OLPs. (ae) CD1 wild-type mice received BrdU 100 min before perfusion. (b) Representative P4 Sox2Olig2 BrdU cell. Scale bar, 10 mm. (c) Representative P16 Sox2 Olig2 BrdU cell. Scale bar, 10 mm. (d) Breakdown of basis pontis BrdU cell density by Sox2/Olig2 co-staining, through postnatal development; means.e.m., n 3 mice per timepoint. Sox2 Olig2BrdU cells outnumber any

other cell type (F1,40Z196.91, Po0.0003, Bonferroni-adjusted two-way ANOVA based on cell type and age; tegmentum shown in Supplementary Fig. 7a). (e) P4 tissue from the indicated regions was analysed for the proportion, among total Olig2 BrdU cells, of Sox2 Olig2 BrdU triple-positive cells;

n 3 mice, means.e.m. Unpaired t-test conrmed Olig2 BrdU cells were more often Sox2 in basis pontis than in ventral medulla (P 0.0055),

dorsal medulla (P 0.0123), midbrain tegmentum (P 0.0152) or corpus callosum (P 0.0380), and more often Sox2 in pontine tegmentum than

ventral medulla (P 0.0231) or midbrain tegmentum (P 0.0366). *Po0.05 versus basis pontis; **Po0.01 versus basis pontis; wPo0.05 versus pontine

tegmentum. (fh) ALDH1L1:GFP mice received BrdU 100 min before perfusion. (g) Sox2 Olig2 BrdUALDH1L1:GFP cells (pink arrows) and Sox2ALDH1L1:GFP BrdUOlig2 cells (blue arrow) in P4 basis pontis. Scale bar, 20 mm. (h) BrdUALDH1L1:GFP Olig2 (white arrow) and

BrdU ALDH1L1:GFPOlig2 (blue arrow) subtypes of proliferative astrocytes in P0 tegmentum. Scale bar, 10 mm. (i) Densities of GFP Olig2BrdU cells in ALDH1L1:GFP pons, and of Sox2Olig2 BrdU cells in wild-type pons; means.e.m., n 3 mice per strain per timepoint. (j,k) CD1 wild-type mice

age P4 were given BrdU as in a, perfused with short postx (100 min, 4 C), cryosectioned, and co-stained as indicated. (j) Representative basis pontis OLP; scale bar, 10 mm. (k) PDGFRa was expressed in B80% of Sox2 Olig2BrdU progenitors; n 3 mice, means.e.m. (ln) Sox2:GFP mice received BrdU

100 min before perfusion. (m) P4 pons tissue co-stained as indicated. Yellow arrows indicate representative BrdU GFP Sox10 triple-positive cells. Scale bar, 15 mm. (n) Sox2:GFP pons regions aged P4 or P45 were analysed for the proportion, among proliferative OLPs (Sox10 BrdU), of cells expressing

Sox2:GFP; this percentage was greater in P4 basis pontis than tegmentum (P 0.0026, unpaired t-test); n 3 mice per timepoint, means.e.m.,

**Po0.01.

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Olig2 and Sox2 dene proliferative progenitor subclasses. To further dene the nature of proliferative pontine glial precursors, we used the markers Sox2 and Olig2, which can be expressed in

tumour-propagating glioma cells39,40. Using wild-type mice, we delivered a single dose of BrdU 100 min before perfusion (Fig. 8a), and performed co-immunouorescent labelling of

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Figure 9 | Postnatal Sox2 progenitors give rise to B95% of adult pons OLs. (ae) Short-term Sox2CreER fate mapping. Sox2CreER;Ai14 pups were given tamoxifen (by maternal gavage) at age P2 and P3, and EdU intraperitoneally at age P8, 100 min before perfusion. (bd) TdTOlig2 cell types observed in P8 basis pontis; scale bars 20 mm. (b) TdTOlig2EdU Sox10 proliferative OLPs (arrows). (c) TdTOlig2 Sox2 (cyan arrows) and

TdTOlig2Sox2 cells (magenta arrows). (d) TdTOlig2 APC MBP myelinating OLs (arrowheads). (e) Quantitation of the proportion of each P8 cell type shown in (bd) that coexpress TdT, demonstrating origin from postnatal Sox2 progenitors. Data shown are from basis pontis. E EdU,

S10 Sox10, S2 Sox2, O2 Olig2. Graph shows individual replicates with means.e.m. of n 3 mice. (fh) Long-term Sox2CreER;AI14 fate mapping.

Tamoxifen induction was performed in one cohort by maternal gavage at P2 and P3, and in a second cohort by IP injection at P10; these two cohorts were perfused at P60. Another cohort received tamoxifen at P60 and was perfused at P120. (g) TdTOlig2 APC mature OLs in adult basis pontis of P2P3 tamoxifen-induced Sox2CreER;Ai14 mice; scale bar, 20 mm. (h) Quantitation (by pons region and tamoxifen timing) of the proportion of mature OLs (APCOlig2) that coexpress TdT, demonstrating origin from Sox2 progenitors at the indicated postnatal ages. A cohort of no-tamoxifen control mice showed TdT expression inB1% of adult pons OLs. Graph shows individual replicates with means.e.m. of the indicated n mice. **Po0.01, ***Po0.001, ****Po0.0001 (unpaired t-test, versus the corresponding region of P2P3 dosed mice).

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BrdU, Olig2 and Sox2 (Fig. 8be, Supplementary Figs 7a and 9a,b). The largest pool of BrdU cells co-expressed Sox2 and Olig2 (Fig. 8b,d; Supplementary Fig. 7a). During the

P4 proliferative peak, the proportion of BrdU Olig2 cells coexpressing Sox2 was greater in basis pontis than in ventral or dorsal medulla, midbrain tegmentum, or even corpus callosum; and likewise a greater proportion co-expressed Sox2 in pontine tegmentum than in midbrain tegmentum (Fig. 8e). Thus the postnatal mouse pons was particularly enriched in Sox2 Olig2 BrdU progenitors. The remaining progenitors included a Sox2 Olig2 BrdU population peaking at P4, a

Sox2 Olig2 BrdU population (Fig. 8c,d, Supplementary Fig. 7a) peaking at P8, and a small Sox2 Olig2 BrdU population most of which lined blood vessels and had elongated nuclei consistent with endothelial cells. The Sox2 Olig2 BrdU population likely corresponds to proliferative astrocytes, as immunostaining revealed broad Sox2 expression in ALDH1L1:GFP cells (Supplementary Fig. 9c), and as its numbers (Fig. 8d) were comparable to

ALDH1L1:GFP BrdU cells (Fig. 7d, Supplementary Fig. 7c).

The Sox2 Olig2BrdU cells were predominantly not astrocytes, because in ALDH1L1:GFP mouse pons, Sox2 Olig2

BrdU cells were usually GFP (Fig. 8f,g; Supplementary Fig. 9b). While a subpopulation of BrdU ALDH1L1:GFP astrocytes expressed Olig2 (Fig. 8h), such cells were sparse compared with the BrdU Olig2 Sox2 progenitors measured in wild-type mouse pons (Fig. 8i, Supplementary Fig. 7c). The total Olig2 BrdU population, measured as the sum of Sox2

Olig2 BrdU and Sox2 Olig2 BrdU populations, showed similar numbers to committed OLPs (Fig. 7d): in basis pontis, a sustained peak from P4 (17610 cells per mm2) to P8 (1734 cells per mm2); in tegmentum, a peak at P4 (14740 cells per mm2). And remarkably, at P4, nearly all BrdU Olig2 cells co-expressed Sox2: 90.4%0.4% in basis pontis and 82.6%3.5% in tegmentum (Fig. 8e). Altogether, this suggested that the committed OLP population included many Sox2 Olig2 cells.

Sox2 was long considered a marker of stem cells, but has recently been documented in embryonic OLPs of forebrain and spinal cord41. We therefore performed immunostaining in P4 ALDH1L1:GFP tissue to test whether pontine Sox2 Olig2 cells co-expressed the canonical OLP marker PDGFRa, or the astrocyte reporter ALDH1L1:GFP (PDGFRa and ALDH1L1:GFP were 99% mutually exclusive in pons, Supplementary Fig. 9f). Remarkably, 89.8%1.7% of Sox2 Olig2 cells in basis pontis were PDGFRaALDH1L1:GFP (Supplementary Fig. 9df).

NG2 protein was similarly localized to Sox2 Olig2 ALDH1L1:GFP cells (Supplementary Fig. 9g). Next, examining CD1 wild-type mice, we found PDGFRa co-expressed in 80.5%3.0% of basis pontis Sox2 Olig2 BrdU cells, and in 83.6%2.1% of tegmentum Sox2 Olig2 BrdU cells (Fig. 8j,k). To test Sox2 coexpression with a third marker of committed oligodendroglia, Sox10, we obtained mice carrying a Sox2:GFP knock-in allele42. We performed a single injection of BrdU 100 min before perfusion at P4 or P45 (Fig. 8l), and co-stained pons sections for BrdU, GFP, and the oligo-lineage marker Sox10 (Fig. 8m). We found that 70.5%3.0% of Sox10 BrdU cells co-expressed Sox2:GFP in the P4 basis pontis, and 43.7%2.6% in P4 tegmentum, while

Sox10 BrdU cells in both pons regions showed roughly 50% Sox2:GFP expression at P45 (Fig. 8n). Altogether, these results indicate that the postnatal pons is rich with Sox2 Olig2 proliferative OLPs, and also contains a smaller, later-peaking,

Sox2 Olig2 proliferative population.

Postnatal Sox2 cells produce over 90% of adult pons OLs. The presence and timing of Sox2 and Sox2 subpopulations of

proliferative Olig2 cells suggested a possible lineage progression of Sox2 Olig2 , to Sox2 Olig2 , to myelinating oligodendrocytes. To test whether postnatal Sox2 cells produced Sox2 Olig2 cells and mature OLs, we used

Sox2CreER;Ai14 reporter mice. We delivered tamoxifen by maternal gavage at P2P3, followed by a single dose of EdU 100 min before perfusion at P8 (Fig. 9a). We found that in basis pontis, TdT cells accounted for: 99.3%0.7% of Olig2

Sox10 EdU proliferative OLPs (Fig. 9b,e); 97.8%2.2% of Olig2Sox2 cells and 89.7%1.6% of Olig2 Sox2

cells (Fig. 9c,e); and 94.5%2.1% of APC Olig2MBP myelinating OLs (Fig. 9d,e). These results support the hypothesis that postnatal Sox2 progenitors generate all stages of the oligodendrocyte lineage. To determine the extent to which postnatal Sox2 progenitors contributed oligodendrocytes to the mature pons, we delivered tamoxifen to Sox2CreER;Ai14 mice at

P2P3, P10, or P60 (Fig. 9f), perfused 5060 days later, and co-stained for TdT, Olig2, and APC. Tamoxifen treatment at P2 P3 led to TdT expression in 97.3%0.5% of APCOlig2

OLs in P60 basis pontis, and in 94.1%0.4% of OLs in P60 tegmentum (Fig. 9g,h). Delivery of tamoxifen at P10 labelled69.0%3.4% of P60 basis pontis OLs and 75.5%1.8% of P60 tegmentum OLs. Delivery of tamoxifen to P60 mice labelled only 13.0%2.6% of P120 basis pontis OLs (Fig. 9h) and14.3%4.3% of P120 tegmentum OLs. This is consistent with the lower level of proliferative Sox2 Olig2 cells in adult pons (Fig. 8d). A no-tamoxifen control showed TdT expression in only1.01.2% of P60 pons OLs (Fig. 9h), indicating low spontaneous background recombination.

The Sonic Hedgehog pathway drives embryonic oligodendrogenesis24,43; its effector Gli1 has been previously proposed as a postnatal pons progenitor cell marker5, and postnatal Gli1 forebrain progenitors are known to produce

OLs44. We therefore tested if pons OLs were derived from Gli1-expressing progenitors. We found that postnatal tamoxifen treatment at P23 or P10 in Gli1CreER;Ai14 mice45 yielded no TdT Olig2 APC OLs in adult pons, but sparse TdT astrocytes positive for S100b and/or GFAP (Supplementary Fig. 10). Thus Gli1-expressing cells at P23 or P10 do not contribute to postnatal pontine oligodendrogenesis, by contrast with Sox2 cells. Tamoxifen-treated Sox2CreER;Ai14 mouse pons also contained TdT astrocytes (Supplementary Fig. 10c), reecting pontine astrocytes common Sox2 expression (Supplementary Fig. 9c). In addition to that expected result, our fate mapping experiments demonstrate that postnatal Sox2 progenitors populate the adult pons with B95% of its oligodendrocytes.

DiscussionWe have shown here that, as in humans3, the mouse pons grows markedly after birth, driven by a single wave of proliferation. We identied Sox2 Olig2 parenchymal progenitors as the main proliferative progenitor population, and found that postnatal Sox2 cells give rise to nearly all adult pons OLs.

The postnatal mouse basis pontis quintupled and the tegmentum quadrupled in size, placing the pons among the brain regions with the most pronounced postnatal growth comparable to that of cortex and hippocampus, but less than that of cerebellum27,28. As in humans3, the mouse basis pontis experienced more rapid growth and greater proliferation than the tegmentum. There were also interesting differences between human and mouse pons: at birth, the human basis pontis already contains thin myelinated bres, and proliferation declines after birth; however, the mouse basis pontis experiences its onset of myelination and decline in proliferation during the second

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postnatal week. The P4 proliferative peak may therefore correspond to a pre-term peak proliferative stage in human pons, which has not yet been studied.

We found that postnatal pontine growth did not involve the addition of new neurons, but rather correlates with a dramatic increase in the oligodendroglial lineage. The basis pontis at P16 contained 18 times as many oligodendroglia as at P0, and the tegmentum at P16 contained 10 times as many as at P0, suggesting that basis pontis OLPs undergo at least four rounds of postnatal division and tegmentum OLPs undergo at least three. However, the expansion might be carried out by subpopulations dividing ve times or more. It is also possible that the population expansion is even greater than observed, but appears reduced due to cell death37 or migration out of pons.

We dened three compartments of postnatal progenitor cell proliferation in the pons: VZ, midline and parenchyma. The VZ and midline contributed only a few cells, generally close to these proliferative domains. The parenchymal compartment generated the majority of new cells; some progenitors were proliferative astrocytes, but a greater number corresponded to proliferative OLPs. BrdU Sox2 Olig2 OLPs were the largest parenchymal progenitor pool, and postnatal Sox2 progenitors produced B95% of adult pons oligodendrocytes. These observations suggest that Sox2 progenitors play a key role in postnatal oligodendrogenesis. A prior study suggested that Sox2 acts in embryonic spinal cord oligodendroglia to block maturation and myelination during the OL stage41; however, we observed that a sizeable subset of postnatal pontine Olig2 cells downregulate

Sox2 while still proliferating, that is, as OLPs. We found a small subpopulation of Sox2 Olig2 progenitors that continued to proliferate in adult mouse pons. Interestingly, Sox2 Olig2 cells have also been described in adult human white matter46. Our

Sox2CreER fate mapping demonstrates that adult Sox2 cells continue to generate oligodendrocytes in vivo.

The functions of sustained postnatal pontine growth and oligodendrogenesis remain unknown. Oligodendrogenesis is regulated by neuronal activity47,48, so the late formation of oligodendrocytes may be linked to postnatal neural activity owing through the pons. By delaying the onset of myelination, the pons might allow experience to guide which circuits become myelinated. It is appealing to speculate that the maturation of cells in the basis pontis is developmentally coordinated with the maturation of cerebellar target regions, whose growth is mostly postnatal. Interestingly, the middle cerebellar peduncle is among the last tracts in the brain to be myelinated27. Given the prominence of motor pathways in the pons, sustained postnatal oligodendrogenesis may allow the development of high-speed circuit conductances in an experience-dependent manner during a critical period of acquisition of motor coordination. Similarly, experience-dependent circuit enhancement could occur in the autonomic regions of the growing postnatal tegmentum that undergo later myelination.

Tumour incidence may be correlated with a tissues normal developmental proliferative activity49. Our ndings may partly explain the ponss susceptibility to tumour formation. The pons is the site where Diffuse Intrinsic Pontine Glioma (DIPG), the most lethal paediatric CNS tumour4, appears. It is suspected that DIPGs have a progenitor cell origin50. This progenitors identity remains uncertain. OLPs have been shown as a cell of origin for some paediatric forebrain gliomas51,52 and adult gliomas53,54. DIPGs frequently express Olig2 and Sox2 (ref. 55); our nding of PDGFRa coexpression in the vast majority of P4

BrdU Olig2 Sox2 cells is notable given that PDGFRA is commonly amplied in DIPG55,56. Most DIPGs also contain a mutation in Histone H3 at lysine 27 (refs 57,58), which might epigenetically trap cells in a progenitor state50 and prevent

repression of Olig2 or Sox2; one study showed that a H3K27M DIPG patient tumour-derived cell line had increased Olig2 expression correlated with decreased H3K27 trimethylation at the OLIG2 locus, when compared with mouse forebrain-derived neurospheres59. All this suggests that the BrdU Olig2 Sox2

OLPs, which peak at P4 and are enriched in basis pontis, are a prime candidate for DIPG cell of origin. However, despite the ventral preference for growth and proliferation (present study and ref. 3) and the propensity of DIPG to spread ventrally60, we cannot rule out that some DIPGs might originate dorsally. We observed signicant OLP proliferation in the tegmentum at slightly earlier stages than basis pontis; interestingly, a fraction of DIPGs express the dorsal marker Pax3 (ref. 61). We also cannot exclude that DIPG might originate from astrocytes. Astrocytes accounted for B14 of proliferation in P4 basis pontis; all astrocytes express Sox2, and a subpopulation coexpress Olig2. And nally, it is possible that DIPG may originate prenatally. In one study on embryonic oligodendrogenesis, it was shown that rhombomere 4, within the pons, produces more oligodendroglia than neighbouring segments26. In light of our postnatal observations, the embryonic pattern of OLP production26 may prepare the pons for its dramatic postnatal growth, and may render the pons rich in potentially gliomagenic progenitors.

The typical presentation of DIPG at 59 years led one group to hypothesize a second peak of progenitor cells in middle childhood5. However, when we examined the postnatal ponss normal course of proliferation, we found only a single peak: at birth in human3 and at P4 in mouse (present study). Furthermore, a single proliferative peak was evident for each progenitor cell population. While we cannot exclude that a tumour might arise from the few proliferative cells that persist to later ages, proliferation data favour an origin for DIPG during the single, early-childhood proliferative peak, from Sox2 Olig2 progenitors.

This study demonstrates that the postnatal mouse pons undergoes a dramatic increase in size. We have identied proliferative oligodendroglia and astroglia in postnatal pons parenchyma, shown that pons OLPs have early Sox2 and later

Sox2 stages, and demonstrated that B95% of pons oligodendrocytes derive from postnatal Sox2 progenitors. This dramatic pontine cell addition may be linked to the acquisition of key developmental milestones in children, and may predispose the pons to tumour formation. The progenitor cells identied here represent candidates for targeting in future tumour models.

Methods

Animals. All animal procedures were performed in accordance with NIH guidelines. Animal protocols were approved and supervised by the UCSF IACUC. Transgenic strains, uses and sources are listed in Supplementary Fig. 1. Transgenic mice were on a mixed background containing CD1 and C57BL/6. Wild type animals in all gures were CD1 (Charles River, Wilmington, MA), except for Fig. 7eh in which wild type represents GFP-negative littermates of the ALDH1L1:GFP mice used elsewhere in those panels. Mice of both sexes were used. The only species used was Mus musculus. BrdU (Sigma-Aldrich, St Louis, MO) was dissolved in sterile phosphate-buffered saline (PBS) and delivered by intraperitoneal injection at a dose of 50 mg kg 1; EdU (Invitrogen, Waltham, MA) was prepared identically and delivered at an equimolar dose, 41 mg kg 1. Tamoxifen was dissolved in sterile-ltered corn oil and delivered by maternal gavage at 5 mg per day, or intraperitoneally into mice P10 or older (4 mg per 30 g body weight). Neonatal intracerebroventricular viral injections were performed in P1 mice using the following stereotaxic coordinates: (2.0 mm posterior from intraocular line,0.8 mm lateral from midline, 1.5 mm deep from skin), with a microinjection needle positioned vertically (0 degrees). 1 ml of virus (Adeno-CMV-Cre, Vector Biolabs, 1010 p.f.u. ml 1; RCAS-GFP, B2 107 p.f.u. ml 1) was delivered into lateral

ventricle. Direct injection into the fourth ventricle could have risked accidental puncture of the ventricular surface and labelling of pons parenchyma, as well as inadvertent contact with cerebellar mossy bre axons from the precerebellar pontine nuclei. Transcardial perfusion was performed as previously described62; most samples underwent post-xation overnight at 4 C, but for samples used

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in PDGFRalpha and NG2 antibody staining (Fig. 8j,k, Supplementary Fig. 8dg), post-xation was limited to 100 min at 4 C so as to preserve antigenicity.

Histology. Because of the placement of the mesencephalic and pontine exures, a standard coronal section63,64 cannot contain the full dorsalventral extent of the pons, from basis pontis through tegmentum up to the 4th ventricle. Therefore, for many experiments (including parts or all of Figs 1,2,4 and 6, and Supplementary Fig. 7) we blocked tissue in an oblique plane, orthogonal to the spinal cord, illustrated in Fig. 1a. This plane contains basis pontis, tegmentum and fourth ventricle, allowing measurement of pons size and comparisons of basis pontis and tegmentum in the same section. For studies of proliferation and/or the ventricular zone, we blocked sections in the standard coronal plane, allowing a wider ventricular space to separate brainstem from cerebellum. For viral fate mapping following lateral ventricle injection (Fig. 5 and Supplementary Fig. 6), sections were coronal or sagittal. For all experiments except PDGFRalpha and NG2 protein staining, tissue sections were prepared at 50 mm thickness on a sliding-freezing microtome (Leica Microsystems, Buffalo Grove, IL), following cryoprotection in 30% sucrose in PBS 0.1% sodium azide, and subjected to immunohistochemistry

as oating sections. For PDGFRalpha and NG2 protein staining, tissue sections were prepared at 30 mm thickness on a Cryostat (Leica), following cryoprotection as above, embedding in O.C.T. (Sakura, Torrance, CA), and frozen storage at

80 C; tissue was collected on SuperFrost Plus slides (Fisher Scientic, Hampton, NH) and subjected to immunohistochemistry on the slides. Some stains required antigen retrieval in 10 mM citrate buffer (pH6.0) at 95 C for 1020 min or 0.3 M glycine buffer (pH7.4) at 56 C for 45 min. All sections were blocked for 3060 min at room temperature in buffer containing PBS, 0.1% Triton X-100 (Fisher Scientic), and 25% species-specic serum (depending on host species of secondary antibody); antibodies were diluted in blocking buffer for primary antibody incubation (overnight at 4 C) and secondary antibody incubation (2 h at room temperature); rinses were performed in PBS/0.1% Triton. Primary antibodies were applied simultaneously, except for anti-BrdU (described below).

Antibodies. Primary antibodies and dilutions are described in Supplementary Fig. 2. Secondary antibodies were from Invitrogen or Jackson Immunoresearch (West Grove, PA), raised in donkey (if one primary antibody in the co-stain was raised in goat) or in goat (in all other cases). IgG subclass-specic secondary antibodies were used where available; secondary antibodies were conjugated to Alexa Fluor 488, 546, 555, 568, 594 or 647 dyes (Invitrogen), or to DyLight 488, Cy3 or Cy5 dye (Jackson Immunoresearch), and all secondary antibodies were used at 1:750 dilution. DAPI (1:5,000; Sigma) was added during secondary antibody incubation; in samples that would subsequently be immunostained for BrdU, DAPI was deferred till the nal secondary antibody incubation.

Thymidine analogue detection. For BrdU co-staining, a sequential protocol was used in which non-BrdU antigens were labelled by primary and secondary antibodies as above; then, uorophores were xed into tissue by 15 min in 4%PFA, followed by denaturation with 2 N HCl at 37 C and quenching in boric acid buffer pH8.5 as previously described31, and nally, blocking and immunostaining with anti-BrdU primary and uorophore-conjugated anti-rat secondary antibodies. For EdU detection, the Click-iT EdU Alexa Fluor 647 kit (Invitrogen) was used before immunostaining according to manufacturers instructions. Control stains were performed to verify that BrdU and EdU methods labelled cells in a distribution comparable to Ki67 immunostaining.

Imaging. Tiled images of entire sections were collected on a Zeiss Axiovert 200 M inverted epiuorescence microscope under 5 or 10 objectives

(Carl Zeiss, Pleasanton, CA), using the Virtual Tissue module of NeuroLucida/ StereoInvestigator software (MicroBrightField, Williston, VT), an automated stage controller (MicroBrightField), and an AxioCam (Carl Zeiss) or HamamatsuOrca camera (Hamamatsu, Shizuoka, Japan). High-resolution images were acquired on SP5 Confocal Microscopes (Leica), equipped with LAS AF software, under a 20 objective and optical zoom to resolutions between 0.757 mm px 1

and 0.278 mm px 1. Fluorophores were excited by sequential scans using xed-wavelength or white-light lasers, with emission detection windows chosen to avoid bleed-through in single-uorophore controls. Individual image channels were merged into colour images in batches using CellProler software65, or for few-image experiments, manually using Adobe Photoshop (Adobe Systems, San Jose, CA). All images shown are representative of nZ3 mice.

Size measurements. DAPI-stained mouse pons sections, oriented in the oblique plane described above, were collected as tiled images in NeuroLucida as described, then pons regions were measured ofine in NeuroLucida software by drawing contours around basis pontis and whole pons. Basis pontis was dened as pons proper66, that is, pontine grey nuclei plus adjacent white matter tracts (transverse fasciculus pontis, longitudinal fasciculus pontis, and middle cerebellar peduncle in its ventralmost aspect, that is, excluding portion contacting tegmental nuclei). Pontine tegmentum was dened by subtraction of basis pontis from whole pons. Volumes for basis pontis and tegmentum were computed as the sum of respective

regional areas in every second section through pons, times the distance between

sections (100 mm 50 mm section thickness every second section). For the size

study, we used CD1 wild-type mice at ages P0 (n 3), P4 (n 4), P10 (n 3),

P16 (n 3), P24(n 3) and P64 (adult, n 3).

Tracing of myelinated tracts. The tracing in Fig. 2a, showing major white matter tracts in mouse pons, was obtained by ofine NeuroLucida tracing of a MBP-stained P16 pons section in the oblique orientation. Tracing was exported as a PostScript le and imported as vector art into Adobe Illustrator (Adobe Systems).

Proliferation maps. Tiled images of mouse brainstem sections, oriented in coronal plane to better distinguish VZ of 4th ventricle, were co-stained for BrdU, Vimentin, and GFAP, then imaged in StereoInvestigator software and traced ofine. Markers were placed at the location of every BrdU nucleus, with distinct markers for cells in VZ, cells contacting Vimentin processes along the midline, cells contacting

GFAP processes branching off the midline, and cells in parenchyma. Maps were exported as postscript les and imported as vector art into Adobe Illustrator.

Proliferation measurements. Mouse brainstem sections were immunostained for BrdU and other markers as described above. For VZ proliferation (Fig. 3f), we used StereoInvestigator to perform live counting under a 10 objective, identifying

BrdU cells in VZ by their overlap with the dense DAPI zone along the fourth ventricle; ventricular surface length was also measured live in StereoInvestigator.

We analysed every third section (in ages P0P12) or every sixth section (in ages P16 and up) through the pontine VZ. Density was computed as the ratio of BrdU

VZ cells to VZ length. For measurements of proliferation density among parenchymal populations (Figs 3,7 and 8; Supplementary Figs 3 and 7), counting was done using confocal images acquired as overlapping individual elds at0.505 mm px 1 resolution spanning the entire (lateral-medial-lateral) extent of basis pontis or pontine tegmentum; we used StereoInvestigator for manual alignment of individual elds, region-area measurements and counting of BrdU cells and colocalized markers (Sox2, Olig2, GFP, Sox10 and DsRed). For pons parenchyma, proliferative cell density was computed as the ratio of BrdU cells to area of basis or pontine tegmentum. For the pontine midline domain, which was dened as the medial white matter space in the section and at all ages was within 50 mm of the midline, proliferative cell density was dened as the ratio of BrdU cells to length of midline. For midbrain, medulla and forebrain regions, proliferation density was measured using a single confocal eld per animal, taken at the following sites: superior colliculus (midbrain tectum), dorsal raphe nucleus (midbrain tegmentum), interpeduncular nucleus (ventral midbrain), prepositus hypoglossal nucleus (dorsal medulla), inferior olivary nucleus (ventral medulla) and dorsal to the anterior horn of the lateral ventricle (corpus callosum and neocortex, both imaged in the same section). We used n 3 animals per timepoint,

and up to 10 timepoints per strain: wild-type and NG2:DsRed mice were aged at 4-day intervals from P0P32, plus P64 (adult); ALDH1L1:GFP mice were aged at 2-day intervals from P0P12, plus P16, P20 and P64 (adult). For quantitation of proliferative OLPs in Sox2CreER fatemap (Fig. 9e), counts of P8 EdU Olig2

Sox10 cells were performed similarly in basis pontis, and the percentage of those cells coexpressing TdTomato (TdT) was reported.

BrdU fatemap counts. BrdU labelled cells in the pulse-chase experiment(Fig. 7eh) were imaged and counted ofine in basis pontis and tegmentum as in acute proliferation experiments (described above). Astrocyte counts were from ALDH1L1:GFP mice and included cells that were ALDH1L1:GFP BrdU and/or

GFAP BrdU . Oligodendroglia counts were from wild-type (GFP ) littermates of the GFP mice and included cells that were Olig BrdU and/or APC

BrdU . n 3 mice were used per strain.

Percentage counts and non-proliferative cell density counts. For measurements of the Sox2:GFP subpopulation of Sox10 BrdU cells at P45 (in Fig. 8l), overlapping confocal elds spanning the basis pontis or pontine tegmentum were acquired from one section per animal at resolution of 0.505 mm px 1; image alignment and exhaustive counting were performed ofine in StereoInvestigator.

For P4, the same procedure was performed, except due to the abundant proliferation, cells were selected by systematic uniform random sampling using the Area Fractionator probe and an area sampling fraction of 1/16. n 3 animals were

used per timepoint. The delity of the Sox2:GFP reporter, previously demonstrated in ref. 42, was conrmed in pons by control co-immunostain of GFP and Sox2 protein. Density counts for the PDGFRa/ALDH1L1:GFP/Sox2/Olig2 costain (Supplementary Fig. 9f) were performed using the same sampling fraction and n, and a single confocal eld for each region. For quantitation of PDGFRa coexpression among Sox2 Olig2 BrdU cells (Fig. 8k), exhaustive counts were performed from a single confocal eld per animal in each of basis pontis (encompassing midline, transverse fasciculus pontis, pontine nuclei and corticospinal tract), tegmentum (adjacent to fourth ventricle) and neocortex (dorsal to anterior horn of lateral ventricle). For counts of non-proliferative cells in short-term Sox2CreER;Ai14 fate mapping (Fig. 9e), systematic uniform random sampling within basis pontis was similarly performed ofine, using confocal images from

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n 3 animals and an area sampling fraction of 1/16; average numbers of cells

assessed for TdT positivity per animal were 90 myelinating OLs (range 65 to 123) and 83 cells positive for Olig2 and/or Sox2 (range 67104). For measurements of the TdT percentage of APC Olig2 oligodendrocytes in adult Sox2CreER;Ai14 mice (Fig. 9g), systematic uniform random sampling through basis pontis or tegmentum was performed live under a 20 objective using the Optical Fractionator

probe; the number of OLs counted per region per animal averaged 193, with minimum of 106 and maximum of 382. nZ3 animals were used per cohort, with specic n indicated in the gure.

Stereologic counts. For counts of oligodendroglia, we performed co-immunouorescent labelling of Olig2, Sox10, and APC, followed by live systematic uniform random sampling under a 20x objective using the Optical Fractionator probe in StereoInvestigator. OLs expressed APC plus at least one of Olig2 and Sox10; OLPs expressed both Olig2 and Sox10 but not APC. Thus a cell was only considered oligodendroglial if it expressed at least two of the three markers. For all samples the counting frame was 50 50 mm, and cell nuclei were

used to dene cell location with respect to the optical disector. Average mounted section thickness varied with age from 30 to 50 mm. Because of pons growth over postnatal development, and also because of the 5x size difference between mouse tegmentum and basis pontis, we chose the sampling parameters on a per-region, per-animal basis, to count an average of at least 200 cells per region per animal. Grid step size varied from 200 200 mm (at P0) to 350 350 mm (at P64) in basis

pontis, and from 480 mm (at P0) to 800 mm (at P64) in pontine tegmentum; section interval varied from 4 (at P0) to 12 (at P64). The resulting number of oligodendroglia counted per basis pontis averaged 204, with a minimum of 83 and maximum of 305; the number of oligodendroglia counted per tegmentum averaged 242, with a minimum of 127 and maximum of 378. Stereologic counts of OLs and OLPs in basis pontis and tegmentum were obtained from three separate animals per timepoint, and mean counts and standard errors were computed from those three replicates.

Statistics. Means, standard errors, and P-values (by two-tailed unpaired t-test) were computed in Microsoft Excel (Microsoft, Redmond, WA). Data were graphed in Excel or GraphPad Prism (GraphPad, La Jolla, CA). Three-way and two-way analysis of variance (ANOVA) was performed in Stata 13 (StataCorp, College Station, TX). Bonferroni correction was performed for multiple pairwise ANOVA comparisons of proliferation among regions (Fig. 3, Supplementary Fig. 3) and cell types (Fig. 8, Supplementary Fig. 6). For the three-way ANOVA in Fig. 7d, comparing proliferative cell density across ages, regions, and cell types (ALDH1L1:GFP BrdU astrocytes versus Sox10 NG2:DsRed BrdU OLPs), comparison was limited to the 7 timepoints measured in both strains (P0, P4, P8,

P12, P16, P20, P64); the three additional timepoints uniquely measured in each strain (P2, P6, and P10 in ALDH1L1:GFP; P24, P28, and P32 in NG2:DsRed) were omitted from that calculation. A separate ANOVA was performed pooling single-strain timepoints with both-strain timepoints, in six groups: P0P2, P4, P6P10, P12, P16, P20P32, and P64; P-values from this second ANOVA were as small as or smaller than those produced by the rst. Therefore we reported the rst, more conservative result. For all experiments, a minimum n of three mice per timepoint was chosen based not on power analysis but on past studies of brain region growth28 and hindbrain development26. The obvious differences in tissue size throughout postnatal development mooted attempts at blinding to sample identity. Distributions were assumed to be normal but this was not formally tested. No data points were excluded from analysis.

Data availability. The data that support the ndings of this study are available from the corresponding author on request.

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Acknowledgements

We thank the following investigators for providing transgenic strains used in this study: Lily Jan, UCSF (NG2DsRed); Alexandra Joyner, Memorial Sloan-Kettering Cancer Center (Gli1CreER); Ross Metzger, UCSF (FoxA2CreER). We thank the following investigators for providing antibodies: C. Stiles, Dana Farber Cancer Institute (anti-Olig2 monoclonal and polyclonal); W. Stallcup, Sanford-Burnham-Prebys Medical Discovery Institute (anti-PDGFRalpha polyclonal). We thank the UCSF Library for use of Stata 13 software, Ricardo Romero-Rodriguez for excellent technical support with histology, Saul Villeda for use of a freezing stage, Patrick McQuillen and William Walantus for support with microscopy, Tarja Yvanka de Soysa for pilot stereotaxic injections, and members of the Alvarez-Buylla and Rowitch laboratories for advice on experiments and this manuscript. This work was supported by grants from the Pediatric Brain Tumor Foundation(to A.A.-B. and D.H.R.), National Institutes of Health (NS28478, to A.A.-B.; P01NS083513, to D.H.R. and A.A-B.; NRSA 1F32NS067889-01A1, to M.C.T.), Howard Hughes Medical Institute (to D.H.R.), and John G. Bowes Research Fund (to A.A.-B.). R.A.L. is supported by the UCSF Medical Scientist Training Program, Neuroscience Graduate Program, and Discovery Fellows Program. L.C.F. is a Howard Hughes Medical Institute fellow of the Helen Hay Whitney Foundation. M.C.T. was supported by the UCSF Neurological Surgery Residency Program. A.A.-B. is the Heather and Melanie Muss Endowed Chair and Professor of Neurosurgery.

Author contributions

R.A.L., M.C.T., D.H.R. and A.A.-B. conceived the study. R.A.L., L.C.F., M.C.T., D.H.R. and A.A-B. designed experiments. R.A.L., C.G., J.L.R.-R., L.C.F. and M.C.T. performed experiments. R.A.L. and A.A.-B. analysed the results and wrote the manuscript. All authors reviewed and helped edit the manuscript.

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How to cite this article: Lindquist, R. A. et al. Identication of proliferative progenitors associated with prominent postnatal growth of the pons. Nat. Commun. 7:11628doi: 10.1038/ncomms11628 (2016).

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