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ARTICLE

Received 8 Dec 2015 | Accepted 20 May 2016 | Published 21 Jun 2016

DOI: 10.1038/ncomms12021 OPEN

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration

C. Colin Brinkman1,2,3, Daiki Iwami1,3,w, Molly K. Hritzo2, Yanbao Xiong1,2,3, Sarwat Ahmad1,3, Thomas Simon1,2,3,

Keli L. Hippen4, Bruce R. Blazar4 & Jonathan S. Bromberg1,2,3

Regulatory T cells (Tregs) are essential to suppress unwanted immunity or inammation. After islet allo-transplant Tregs must migrate from blood to allograft, then via afferent lymphatics to draining LN to protect allografts. Here we show that Tregs but not non-Treg T cells use lymphotoxin (LT) during migration from allograft to draining LN, and that LT deciency or blockade prevents normal migration and allograft protection. Treg LTab rapidly modulates cytoskeletal and membrane structure of lymphatic endothelial cells; dependent on

VCAM-1 and non-canonical NFkB signalling via LTbR. These results demonstrate a form of T-cell migration used only by Treg in tissues that serves an important role in their suppressive function and is a unique therapeutic focus for modulating suppression.

1 Center for Vascular and Inammatory Diseases, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. 2 Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. 3 Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. 4 Division of Blood and Marrow Transplantation, Department of Pediatrics, University of Minnesota Cancer Center, Minneapolis, Minnesota 55455, USA. w Present address: Department of Renal and Genito-Urinary Surgery, Hokkaido University, Kita-15, Nishi-7, Kita-ku,

Sapporo 060-8638, Japan. Correspondence and requests for materials should be addressed to J.S.B. (email: mailto:[email protected]

Web End [email protected] ).

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Regulatory T cells (Tregs) help maintain immunological tolerance and resolve inammation following infections1. Treg induction or transfer is of interest for treatment

of a variety of diseases. Treg must migrate to both grafts and lymph nodes (LN) to promote allograft acceptance24. We previously reported that Tregs migrate from blood to islet allografts, then to afferent lymphatics and the draining LN2, and that Treg migration from graft to LN was required for optimal graft survival. Others found that Tregs are the major lymphocyte subset migrating from inamed skin during contact hypersensitivity and that such migration is involved in regulating inammation5. Thus, Treg migration to draining LN via lymphatics is a normal part of the inammatory response and important in inammatory resolution.

In contrast to migration from blood to LN or non-lymphoid tissues, lymphocyte migration from tissues to LN via afferent lymphatics is incompletely understood. The most extensive literature on lymphatic migration regards dendritic cells (DCs)68, with less known about the migration of T cells9, or other cells, such as neutrophils10. In mice, DCs follow CCL21 gradients to lymphatics using the chemokine receptor CCR7, where they enter lymphatic capillaries via aps between overlapping lymphatic endothelial cells (LECs) in a process that does not require integrins or proteolysis11,12.

It had been thought that, like DCs, T cells use CCR7 to exit tissue and access lymphatics13,14. However, recent work found that T cells and DCs use CCR7 differently during migration from afferent lymph to LN, and T cells do not need CCR7 to enter LN from lymph15. Others report that CD4 T cells do not require

CCR7 to exit tissue, enter lymph and inltrate LN while CD8

T cells do16. These conicting reports underscore how little is known about the mechanisms governing T-cell afferent lymph migration. It is also not known if Tregs rely on the same or different mechanisms as non-Treg or DC for lymphatic migration or tissue egress.

Lymphotoxins (LTs) are cytokines related to tumour necrosis factor alpha (TNFa), and function in organizing and maintaining lymphoid organs, and as cytotoxic effector molecules17. There are two LT subunits, soluble a and membrane-bound b, primarily found as a soluble homotrimer of a (LTa3) that binds TNF receptors, or a membrane-bound heterotrimer (LTa1b2) that interacts with the LT b receptor (LTbR)18. LTa1b2 is expressed on activated T, B and natural killer cells18,19, and interacts with LTbR on DC, monocyte lineage cells and stromal cells17.

Murine array data suggest that Tregs express elevated levels of LTa compared with other T cells20. LTbR is required for proper migration of autoreactive T cells during thymic negative selection21, and B cell LTa1b2 contributes to a positive feedback loop that induces CXCL13 in follicular DCs22. LT, likely expressed by DCs, promotes the homeostatic maintenance of high endothelial venules (HEV) adhesion molecule and chemokine expression23,24, yet LTab expressed by T cells has not been described to be directly involved in their migration.

Here we report that Tregs use LTab to stimulate LTbR on lymphatic endothelium for migration to LN via afferent lymphatics. This interaction is not used by non-Treg T cells and is not required for Treg migration from blood through HEV into the LN, or from LN into efferent lymphatics. Tregs, but not non-Treg CD4 T cells, induce rapid growth of lamellipodia-like

projections from LEC but not blood endothelial cells (BECs) in an LTabLTbR-dependent manner. These cytoplasmic membrane projections correlate with altered movements and transmigration patterns of Treg as they travel across LEC. These results demonstrate a novel form of T-cell migration utilized by Treg in tissues that serves an important role in their suppressive function and is a unique target for modulating suppression.

ResultsLT regulates Treg suppressor function in vivo. To determine if LT was important for Treg function, natural Tregs (nTregs) were isolated from young, naive wild-type (WT) C56BL/6J mice or mice decient in LT a (Lta / ), and transferred into diabetic C56BL/

6J recipients under the kidney capsule with BALB/c pancreatic islets. As previously shown2, local transfer of WT nTreg promoted substantial survival prolongation of islet allografts relative to no nTreg transfer by mean survival time (MST) and by log-rank survival curve comparison (Fig. 1a). In contrast, local transfer of Lta / nTreg resulted in no increase in MST or difference by log-rank comparison relative to no transfer and a decrease in survival compared with WT nTreg by MST and log-rank survival comparison (Fig. 1a).

We previously demonstrated that Treg must migrate sequentially from islet allografts to draining LN to prolong islet survival2. Protection was prevented by blocking Treg entry into the graft or egress from the graft into draining LN. As the Tregs were placed under the kidney capsule with the islets in these experiments (Fig. 1a), this left several possible reasons for the defective protection afforded by Lta / nTreg: Lta / nTreg could be defective in suppression; have a proliferative or survival defect; and/or fail to trafc to draining LN, a step essential for islet allograft protection2. To distinguish these possibilities, Treg suppression of CD4 T-cell proliferation was assessed in vitro.

WT and Lta / nTreg equally suppressed proliferation of conventional CD4 T cells to anti-CD3e monoclonal antibody

(mAb), throughout a range of suppressor to responder ratios (Fig. 1b). There were also no signicant differences in expression of Il10 (interleukin 10), Tgfb1, Prf1 (perforin), Gzmb (granzymeB), Ifng (interferon gamma), Cd39, Cd73 and Ctla4 by real-time quantitative reverse transcriptionPCR (qRTPCR) between WT and Lta / nTreg (Supplementary Fig. 1a). Lta / nTreg had no defect in proliferation or viability compared with WT nTreg when stimulated with anti-CD3e and anti-CD28 in vitro (Supplementary Fig. 1b).

Together, these data suggested Lta / nTreg had no defect in suppressor function or predisposition to apoptosis. Treg frequency (Fig. 1c) and number (Fig. 1d) in grafts 4 days after local co-transfer was assessed. There was a trend for higher frequencies and numbers of Lta / nTreg of total CD4 T cells in grafts

compared with WT nTreg (Fig. 1c,d). This suggested that LTa / nTreg did not have a survival defect in vivo. Indeed, there was no difference in the ratio of anti-apoptotic Bcl2 to pro-apoptotic Bcl2l11 (Bim) between WT and Lta / Treg (Supplementary

Fig. 1a). Treg frequency in draining LN was also measured. Lta / nTreg were signicantly less frequent in the graft-draining renal LN compared with WT nTreg (Fig. 1c,d).

When nTreg were transferred intravenously (i.v.) instead of locally, Lta / nTregs were present at a similar percentage and number as WT nTreg in the graft (Fig. 1c,d), while Lta / nTreg representation in draining LN was slightly higher (Fig. 1c,d).

This suggests that Lta / nTreg had no defect in entering the graft or draining LN from blood relative to WT. These results indicated that Lta / nTreg had normal suppressive function in vitro, no gross survival defect, but a profound defect in migration from tissue to draining LN.

Tregs express more LTab compared with other T-cell subsets. To determine if LT might be particularly relevant to Treg, the expression of various LT family members was assessed by ow cytometry and qRTPCR. Using LTbRIg to detect surface LT, freshly isolated nTreg expressed more LT than naive CD4 CD25 CD44loFoxp3 T cells, recently activated

CD4 CD25 Foxp3 T cells, or CD4 CD44hi memory

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a b

P=0.15

Islet allograft survival

Time (days)

P=0.44

100

P=0.71

P=0.77

Untreated

Per cent survival

Lta/ local

WT local

% Divided responder CD4 T cells

0 10 20 30 40 50

No anti-CD3

No Treg

1:8

1:4

1:2

1:1

1:8

1:4

1:2

1:1

Wild type nTreg Lta/ nTreg

Local transfer graft

Local transfer draining lymph node

Wild type Treg Lta/ Treg

Local transfer graft

Local transfer draining lymph node

P=0.01

P=0.15

P=0.37

2.0

2,000

800

Frequency of

transferred Treg (%)

Frequency of

transferred Treg (%)

# Transferred Foxp3+

of CD4 T cells

# Transferred Foxp3+

of CD4 T cells

1.5

P=0.004

1,500

1,000

500

600

1.0

400

0.5

200

0.0

Wild type Treg

Lta/ Treg

Wild type Treg

Lta/ Treg

Wild type Treg

Lta/ Treg

I.v. transfer graft

I.v. transfer draining lymph node

I.v. transfer graft

I.v. transfer draining lymph node

P=0.11 P=0.49

P=0.03

P=0.18

Lta/ Treg Lta/ Treg

0.25

150

Frequency of

transferred Treg (%)

Frequency of

transferred Treg (%)

# Transferred Foxp3+

of CD4 T cells

# Transferred Foxp3+

of CD4 T cells

0.20

100

0.15

0.10

0.05

0.00

Wild typeTreg

Wild type Treg Wild type Treg

Lta/ Treg

Figure 1 | LT regulates Treg suppressor function in vivo but not in vitro. (a) Survival curves for BALB/c islets transplanted into diabetic C57BL/6J recipients monitored by blood glucose. Five untreated from two pooled experiments, ve Lta / Treg recipients from two pooled experiments, six WT

Treg recipients from three pooled experiments. For multiple long-rank comparisons among three curves P 0.0167 was considered signicant for individual

comparisons. *Indicates WT is different than both Lta / and untreated by log-rank comparison (P 0.0007) and mean survival time: 38.71.3 days

(s.e.m.), n 6 WTversus 14.001.1 days n 5 Lta / , Po0.01, and 38.71.3 days WTversus 10.61.3 days n 5 untreated, Po0.01, Tukey post tests

of one-way analysis of variance. (b) In vitro suppression showing per cent of conventional CD4 responder T cells that divided under various conditions. Triplicate wells in each of two experiments, pooled. Statistics from unpaired Students t-tests. (c,d) Islet and draining LN migration. Scatter plots show % (c) or number (d) of all CD4 T cells in indicated organ that are transferred Treg. Four recipients from two experiments for WT and eight from three experiments for Lta / Treg transfer. Statistics from MannWhitney non-parametric tests (bd). Error bars are s.e.m.

Lta/ Treg

Wild type Treg

T cells as judged by both geometric mean uorescence intensity and per cent positive staining (Fig. 2a,b). Lta / nTreg did not bind LTbRIg (Fig. 2a). For Treg culture, naive CD4 T cells and nTreg were ow sorted from Foxp3GFP reporter mice and stimulated with anti-CD3 and interleukin (IL)-2 (activated CD4

and nTreg); anti-CD3, IL-2 and TGF-b1 (induced Treg or iTreg); or anti-CD3, IL-2, TGF-b1 and 5-Aza-20-deoxycytidine (Aza Treg), which we previously showed generated an epigenetically demethylated iTreg with similarities to nTreg25. Cultured Treg subsets all expressed more LT than activated CD4 CD25

non-Treg (Fig. 2c,d), consistent with qRTPCR results, which also showed that none of the Treg expressed substantial Ltbr (LT b receptor), LT family members Tnfrsf14 (herpes virus entry mediator, Hvem), or Tnfsf14 (LIGHT) (Supplementary Fig. 1c). These data suggested that LTa1b2 or LTa3 might have differing importance to the function or trafcking of Treg and non-Treg.

It was possible that Lta / Treg had altered expression of molecules required for T-cell trafcking, and that the effect on

migration was not directly related to LT. However, expression of LFA-1 (CD11a/CD18), CD31, a4b1 (VLA-4), a4b7, aEb7 and aV integrins by ow and Ccr7 and S1pr1 by qRTPCR revealed no differences between WT and Lta / (Supplementary Fig. 2).

This implicated LTa itself as likely involved in and important for Treg migration to LN via lymphatics.

Treg use LTabLTbR for in vivo migration into lymphatics. Lta / Treg lack both LTa3 and LTa1b2. LTa3 interacts with

TNF receptors I and II and LTa1b2 with LTbR. Given the known role of LTbR in development and homeostasis of lymphatic endothelium and lymphoid organs17 we hypothesized that disruption of LTa1b2/LTbR interactions was responsible for the migration defect. To test this, the LTbRIg fusion protein of LTbR with mouse IgG1 was used in the lymphatic migration model of footpad to draining popliteal LN26. LTbRIg blocks interactions of either LIGHT or LTa1b2 with LTbR. Carboxyuorescein

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Lta/ Wild type

nTreg CD4+CD25hiFoxp3GFP+

% Expressing LT

50 800

Naive CD4+CD25CD44loFoxp3GFP

LT gMFI: 195

100

CD4+CD25+Foxp3GFP

LT gMFI: 281

100

Memory CD4+Foxp3GFP-CD44hi

LT gMFI: 168

100

nTreg CD4+CD25hiFoxp3GFP+

LT gMFI: 586

0.49

7.4

18.1

Relative cell number

10.3

35.8

0 102 103 104 105

0 102 103 104 105 0 102 103 104 105 0 102 103 104 105 0 102 103 104 105

LT gMFI: 6.1

Net gMFI LT

***

% Expressing LT

gMFI

600

400

200

CD25+

Naive Foxp3GFP

CD44hi

nTreg

CD25+

Naive Foxp3GFP

CD44hi

nTreg

Activated CD4+ CD25+Foxp3GFP

iTreg CD25hiFoxp3GFP+

Aza Treg CD25hiFoxp3GFP+

Activated nTreg CD25hiFoxp3GFP+

100

Relative cell number

72.3

85.5

85.4

0 0 102 103 104 105 0 102 103 104 105 0 102 103 104 105 0 102 103 104 105

LT gMFI: 921

LT gMFI: 1924

LT gMFI: 1902

LT gMFI: 3617

d % Expressing LT

Net gMFI LT

***

% Expressing LT

100

5,000

4,000

3,000

2,000

1,000

Effector iTreg Aza Treg

Delta gMFI

Activated nTreg

iTreg Effector

Aza Treg

Activated nTreg

0 Effector iTreg Aza Treg

Activated nTreg

Figure 2 | Tregs express more LTab than non-Treg. (a,c) Flow cytometry for LT on freshly isolated (a) or 5-day cultured (c) CD4 T cells from LN and

spleen of Foxp3GFP (a,c) or Lta / Foxp3GFP mice (a) as indicated. Histograms gated on indicated populations. Black histograms: secondary antibody only; blue histograms: MOPC21 control primary; red histograms: LTbRIg stained. Numbers in plots indicate % specic positive staining in LTbRIg-stained minus MOPC21-stained samples. Numbers below are geometric mean uorescence intensity (gMFI) of LTbRIg stained minus MOPC21 stained. Results typical of four experiments in a and three in c. (b,d) Summary data for %LT and gMFI LT from a and c, respectively. *Po0.05, **Po0.01, ***Po0.001 by

repeated measures analysis of variance with Tukey post tests in b and d.

succinimidyl ester (CFSE)-labelled nTreg were pretreated with LTbRIg or control isotype-matched MOPC21 antibody, washed, injected into hind footpads and draining popliteal LN examined 12 h later. LTbRIg pretreatment or use of Lta / nTreg resulted in large and signicant decreases in nTreg migration to the

draining LN (Fig. 3a). Migration of naive non-Treg CD4 T

cells to the popliteal LN was unaffected by LTbRIg or genetic ablation of Lta (Fig. 3b), and migration of CD8 T cells from the footpad to the draining LN was also unaffected by LTbRIg (Supplementary Fig. 3a), indicating that the effect was specic for

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NATURE COMMUNICATIONS | DOI: 10.1038/ncomms12021 ARTICLE

Treg. It was also possible that Lta / Treg migrated through lymphatics normally but did not enter LN normally from lymph.

However, uorescent microscopy of popliteal LN from mice that received footpad injections of WT or Lta / Treg revealed that

when detectable, Lta / Treg were visible in the LN parenchyma and not the subcapsular sinus, and were distributed normally (Supplementary Fig. 3bd). Because Treg do not express Tnfsf14/LIGHT or Ltbr (Supplementary Fig. 1c),

Footpad injection

a b

Footpad injection

0.3

0.2

0.1

0.0

%CFSE+ Treg of all

CD4 T cells

%g CFSE+ non-Treg of

all CD4 T cells

WT nTreg

MOPC21

WT nTreg LTRIg

Lta/ nTreg

0 WT naive MOPC21

WT naive LTRIg

Lta/ naive

c d e

I.v. injection

P=0.82

P=0.6

P=0.56

%CFSE+ Treg of all

CD4 T cells

0.15

0.10

0.05

0.00

2.0

1.5

1.0

0.5

0.0

Ratio transferred Treg

to non-treg

0.6

0.4

0.2

0.0

100

Pre-injection Control IgG

Anti-CD62L

Pre-injection

Control IgG

Anti-CD62L

MOPC21 LTRIg MOPC21

LTRIg

MOPC21 LTRIg MOPC21

LTRIg

LN Spleen

f g h

WT nTreg into WT ears

% of transferred nTreg

MOPC21

LTRIg

Ratio Lta-/- to wild type treg in LN

Touching

Within lymphatics

lymphatics

Not touching lymphatics

Lyve-1 Treg

i j k

Lta/ nTreg into WT ears

WT nTreg into Ltbr/ ears

100

MOPC21

% of transferred nTreg

100

% of transferred non-Treg

WT non-Treg into WT ears

MOPC21

LTRIg

NS NS

Touching

Within lymphatics

lymphatics

Not touching lymphatics

Touching

Within lymphatics

lymphatics

Not touching lymphatics

Touching

Within lymphatics

lymphatics

Not touching lymphatics

WT nTreg

MOPC21

Linear distance travelled Velocity

P< 0.0001

LTRIg

MOPC21

LTRIg

Lta/ nTreg

0 100 200 300

0 100 200 300 0 5 10 15 20

0 5 10 15 20 m min1

Linear distance travelled

Velocity

P=0.26

P=0.75

m min1

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potentially several cell types in tissues in vivo that could express LTbR, we used a previously developed in vitro model to make denitive conclusions about the relevant cells and interactions.We assayed Treg transmigration across the SVEC4-10 LEC line and MS-1 BEC line26. These cells were grown on transwell chamber inserts to produce conuent monolayers, T cells were transmigrated across them and migrated cells in the lower chamber counted. Migration across lymphatic endothelium proceeds only from the basal to the luminal side of the endothelium, but not in the reverse direction26, so SVEC4-10 were grown on the lower sides of transwell inserts (Supplementary Fig. 4). In this orientation, SVEC4-10 layers are referred to as iSVEC4-10 for inverted, and T-cell migration through these layers proceeded in the basal to luminal direction (Supplementary Fig. 4b). LTbRIg reduced transmigration of all

Treg subsets across iSVEC4-10 to CCL19 (Fig. 4a). In contrast, migration of naive or activated non-Treg CD4 T cells across

iSVEC4-10 was not affected. LTbRIg did not block migration of any cell type across MS-1 (Fig. 4b). As expected, Lta / Treg migration across iSVEC4-10 was unaltered by LTbRIg, indicating its specicity (Fig. 4c). These data were in concordance with in vivo footpad, ear pinna, islet allograft and blood to LN migration results (Figs 1c and 3al). Treg migration across iSVEC4-10 to CCL5 (Fig. 4d) and sphingosine-1-phosphate (Fig. 4e) was also inhibited by LTbRIg, indicating the effect was not unique to a single chemo-attractant or receptor. Treg migration across plastic was not blocked by LTbRIg, further proving specicity and suggesting the effects of LTbRIg were not Treg intrinsic, but depended on altering Treg interactions with SVEC4-10 (Fig. 4f). Migration of CD8 T cells, and memory CD44hi CD4 T cells were unaffected by LTbRIg (Fig. 4g,h). Also, blockade of IL-10 and TGF-b1 with antibodies did not alter Treg migration across iSVEC4-10 to CCL19, nor did addition of IL-10 and/or TGF-b1 to CD4 non-Treg alter their migration across

iSVEC4-10 to CCL19 (Fig. 4i,j). Together these results indicated that Treg but not non-Treg migration across lymphatic but not blood endothelium depended in part on the interaction of Treg LTa1b2 with LEC-expressed LTbR.

Treg migration across iSVEC4-10 is regulated by NIK. LTbR stimulation activates three pathways: c-Jun N-terminal kinase (JNK); classical nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB); and non-canonical NFkB27. To determine which pathway regulated transendothelial migration, Tregs were migrated to CCL19 across iSVEC4-10 layers treated with the JNK inhibitor JNK-IN-8 or the classical NFkB inhibitor

BAY 11-7082. These treatments did not decrease Treg transmigration (Fig. 5a). To further conrm the lack of effect of

Figure 3 | Tregs use LTabLTbR for in vivo migration into lymphatics. Resting WT C57BL/6J or Lta / CD25 CD4 cells magnetic bead-enriched,

CFSE-labelled, treated with LTbRIg or MOPC21, washed and injected into footpads (a,b), blood (c,d) or ear pinnae (fk). Popliteal LN and spleens procured 12 h later and analysed by ow cytometry. In all, 912 mice per cell type from 34 experiments in a; 622 mice from 24 experiments in b; 3 mice per group from 1 experiment representative of 2 (c,d). (e) Ratio e670 dye-labelled Lta / nTreg to CFSE-labelled WT nTreg in pre-injection mixture, or after i.v.

injection followed by anti-CD62L or control mAb 18 h later. LN removed and analysed by ow cytometry 18 h later. P values from Wilcoxan signed rank test for pre-injection to control IgG and anti-CD62L; Students t-test for control IgG versus anti-CD62L. In all, 1 pre-injection sample, 6 LN from control IgG treated animals and 9 LN from anti-CD62L-treated animals from 1 experiment, representative of 2. (f,g) Photomicrograph from ear pinna whole mount. LYVE-1 , lymphatics red; CFSE nTreg, green; 20. Scale bar, 50 mm. MOPC21-treated (f); LTbRIg-treated (g). Green arrows indicate Treg in lymphatics

(f) not in lymphatics (g). (hk) Summary of ear pinnae data indicating relationship of transferred T cells to lymphatics. In all, 20250 cells per eld from 1819 20 objective elds per condition derived from 9 ears per condition from 3 independent experiments in h; and 15200 cells per 20 objective eld

from 410 elds from 3 ears per condition in 1 experiment each representative of 2 experiments in ik. (l,m) Summary of Treg movement in ears during 30 min of image acquisition. In all, 1,611 MOPC21- and 3,490 LTbRIg-treated WT nTreg, and 641 MOPC21- and 1,345 LTbRIg-treated Lta / nTreg tracked using Volocity 6.1.1. Results pooled from one 20 eld per ear, 23 ears per condition from 23 independent experiments. *Po0.05, **Po0.01 Kruskal

Wallis and Dunns multiple comparison (a,b), Students t-test (c,d,hm). (ak) Error bars are s.e.m. (l,m) Box and whiskers plots showing minimum, maximum, mean ( ), median (bar), 25th and 75th percentiles. NS, not signicant.

together these results showed that blocking interactions between LTa1b2 on Treg and LTbR expressed by other cells prevents migration into lymphatics and draining LN. LTbRIg did not affect Treg or naive CD4 T-cell migration from blood into

lymphoid organs, as there were no signicant changes in the migration of LTbRIg pretreated Treg from blood into LN or spleen after i.v. injection (Fig. 3c), and the ratio of Treg to co-transferred naive non-Treg CD4 T cells was unaltered by

LTbRIg (Fig. 3d). These data also argue against an Fc-mediated inhibition of Treg migration due to increased binding of LTbRIg to Treg compared with non-Treg.

To determine if LN egress was also regulated by LT, a mixture of WT and Lta / nTreg and non-Treg CD4 T cells was

co-transferred iv into WT mice, 18 h later recipients were treated with anti-CD62L mAb to prevent further LN entry via HEV, and another 18 h later LN and spleen were analysed by ow cytometry. Importantly, the ratio of Lta / to WT nTreg did not differ between pre-injection and control LN, providing further evidence that Treg entry into LN from blood was not regulated by Treg expression of LT (Fig. 3e). Furthermore, if Treg-expressed LT were required for Treg LN egress, anti-CD62L should result in a higher ratio of Lta / to WT Treg than in control animals. However, there were no differences in the ratio of Lta / to WT Treg between control and anti-CD62L-treated

LN (Fig. 3e). Thus, LT on Treg did not regulate Treg LN egress.

The ear pinna model of lymphatic migration was utilized to visualize T-cell encounters with lymphatics. LTbRIg pretreatment of WT nTreg resulted in a lower percentage of Treg within or touching the lymphatics and a greater percentage not touching the lymphatics compared with control treatment (Fig. 3fh). In contrast Lta / nTreg (Fig. 3i), WT nTreg transferred into

Ltbr / ears (Fig. 3j) and non-Treg CD4 T cells (Fig. 3k)

were not affected by LTbRIg. We also tracked the in situ movement of live Treg in ear explants. Treg in the vicinity of lymphatics moved farther and faster when treated with LTbRIg (Fig. 2l and Supplementary Movies 1 and 2). In contrast, movement of Lta / Treg in ear explants was not affected by treatment with LTbRIg (Fig. 2m and Supplementary Movies 3 and 4). These observations were consistent with an essential role for Treg LTa1b2 interactions with LTbR in normal interstitial and/or lymphatic migration, and collectively, these data indicated that nTreg and iTreg, but not non-Treg, CD4 T-cell migration

into lymphatic vessels depended on Treg LTa1b2 interactions with LTbR on stromal or lymphatic cells.

Tregs use LTabLTbR for transmigration across LEC in vitro. We hypothesized that Treg LTa1b2 interaction with LEC LTbR was an important step in lymphatic migration. Since there were

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a b

MS-1 transmigration

iSVEC4-10 transmigration NS

Normalized transmigration

1.25

***

NS NS NS NS

Normalized transmigration

Naive CD8+ T cells NS

1.00

MOPC21

LTRIg

0.75

0.50

0.25

0.00

Naive

CD4+

Activated CD4+

iTreg Aza TregActivated nTreg

0 Naive CD4+

Activated CD4+

iTreg Aza TregActivated nTreg

c d e

iSVEC410 transmigration

iTreg To S1P

NS * ***

1.5

Normalized transmigration

MOPC21 NS

LTRIg

1.0

% transmigrated

Normalized transmigration

0.0

1.0

0.5

0.0

Naive CD4+ LT[afii9825] /

Activated CD4+ LT[afii9825] /

iTreg Aza Treg

Aza Treg LT[afii9825] /

Activated CD4+

iTreg Aza Treg

MOPC21

LTRIg

f g h i

CD44hi CD4+ Aza Treg: iSVEC410

NS NS NS

1.5

Normalized transmigration

MOPC21

Normalized transmigration

LTRIg

1.0

MOPC21

LTRIg

1.0

0.5

0.0

Activated CD4+

MOPC21

LTRIg

Isotype

control

anti-IL-10 anti-TGF-1

WT CD4+ non-Treg: iSVEC410

Control

1.5

Normalized transmigration

1.0

0.5

0.0

IL-10

TGF-1

IL-10+

TGF-1

Figure 4 | Treg uses LTabLTbR for in vitro transmigration across a LEC line in vitro. (aj) Indicated T cells migrated across iSVEC4-10 (a,ce,gj), MS-1 (b), plastic (f), for 4 h to CCL19 (ac,fj), CCL5 (d) or S1P (e) in the presence of LTbRIg or control MOPC21. Migrated T cells in lower wells enumerated and transmigration normalized to MOPC21 shown (aj). A total of 915 individual transwells per condition from 34 experiments (ad,f), 8 individual transwells per condition from 2 experiments (e), 10 individual transwells per condition pooled from 2 experiments (g), 5 individual transwells per condition from 2 experiments (h), 8 individual transwells per condition from 2 experiments (i) and 7 individual transwells per condition from 2 experiment (j). *Po0.05, **Po0.01, ***Po0.001 from unpaired two-tailed Students t-tests (ai) and Bonferroni multiple comparison tests in j. Error bars are s.e.m. NS, not signicant.

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a b c

* NS

Path length

* NS

Velocity

NS NS

3.0

100

1.25

1.0

2.4

Normalized

trnasmigration

Normalized

transmigration

1.00

1.8

0.75

m min1

0.5

0.50

1.2

0.25

0.6

0.00

0.0

MOPC21

TNF BAY 11-7082

JNK-IN-8 LTRIg

+ + +

MOPC21

LTRIg

NIKi

MOPC21

LTRIg

MOPC21

LTRIg

d e

Path length

Velocity

Path length

Velocity

1.8

***

3.0

***

2.4

1.2

1.8

m min1

1.2

0.6

0.0

MOPC21

LTRIg

MOPC21

LTRIg

MOPC21

0 LTRIg

NIKi

MOPC21

LTRIg

NIKi

WT nTreg footpad migration

P=0.0057

Frequency of

transferred Treg (%)

0.15

0.10

0.05

0.00

DMSO NIKi

Figure 5 | Treg migration through and two-dimensional interactions with LEC depend on LTbR-dependent non-canonical NFjB signalling in LEC. (a,b) Aza Treg migrated across iSVEC4-10 to CCL19 in the presence of LTbRIg or control MOPC21. iSVEC4-10 pretreated as indicated. Migration normalized to MOPC21. Six transwells per condition from two experiments in a and nine transwells per condition from three experiments in b. (c,d,e) Aza

Treg two-dimensional movement on iSVEC4-10 10 min (c) or 3 h (d,e) after Treg addition in the presence of CCL19 gradient. Images collected once per minute for 30 min. A total of 78 tracked cells MOPC21, 117 LTbRIg from 1 experiment representative of 4 in c; 100 tracked cells MOPC21, 87 LTbRIg from 1 experiment representative of 2 in d; 155 tracked cells MOPC21, 151 LTbRIg, 118 NIKi from 1 experiment representative of 2 in e. (f) Resting WT C57BL/6J

CD25 CD4 nTreg enriched by magnetic beads, labelled with CFSE, mixed with DMSO or NIKi in DMSO and injected into footpads. Popliteal LN

procured 12 h later and analysed by ow cytometry. Nine mice for each treatment from two experiments in f, *Po0.05, **Po0.01, ***Po0.001 by Bonferroni post test of one-way analysis of variance in a,b and e, unpaired two-tailed Students t-tests in c and d and MannWhitney in f. Error bars are s.e.m. NS, not signicant.

the JNK and classical NFkB pathways on Treg transmigration, iSVEC4-10 were treated with TNFa, which only induces signals via those pathways. While SVEC4-10 have functional TNFa receptors, which permit them to increase expression of cell surface adhesion molecules26, TNFa failed to increase Treg transmigration or reverse LTbRIg-induced inhibition (Fig. 5a). In contrast, pretreatment of iSVEC4-10 cell layers with 4H-isoquinoline-1,3-dione (NIKi), an inhibitor of the non-canonical NFkB-inducing kinase (NIK)28,29, inhibited Treg transmigration. NIKi inhibition was not additive with LTbRIg, suggesting both blocked the same pathway (Fig. 5b).

Since LTbRIg impaired Treg transmigration across lymphatic endothelium, we hypothesized that it altered interactions of Treg with lymphatic endothelium before or during transmigration. To determine how LT engagement regulated migration, Treg movement across iSVEC4-10 layers was assessed by live imaging. If imaging began 10 min after Treg addition, there were no differences in distances travelled or velocities between LTbRIg-treated and control groups (Fig. 5c). However, if imaging was initiated 3 h after Treg addition, LTbRIg resulted in increased Treg distances and velocities compared with controls (Fig. 5d),

suggesting time- and LT-dependent TregLEC interactions resulting in Treg slowing, adhesion and transmigration. These ndings are consistent with the ear live imaging data (Fig. 3l). NIKi pretreatment, like LTbRIg, also led to increased distances and velocities of Treg movement, again suggesting that both agents blocked the same pathway (Fig. 5e). We also co-injected NIKi at 10 mg kg 1 into footpads with WT nTreg and found that

NIKi inhibited Treg migration to draining LN (Fig. 5f). These ndings support a role for NIK controlling Treg interstitial and lymphatic migration in vivo and in vitro.

Tregs induce protrusion on the basal surface of LEC. Leukocytes interact dynamically and intimately with endothelial cells during transmigration, engaging multiple receptors and ligands and involving downstream cytoskeletal changes. To probe TregSVEC4-10 interactions over time, iSVEC4-10 layers were stained with phalloidin to reveal lamentous actin (f-actin). iSVEC4-10 grown for 23 days on transwell inserts formed conuent monolayers with intense f-actin staining on the lower or luminal surface, and thin f-actin protrusions invading the

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a b

SVEC4-10 + Basal medium

SVEC4-10 phalloidin staining

*** ***

Basal Mid-layer Luminal

Basal Mid-layer Luminal Basal Mid-layer Luminal

Basal Mid-layer Luminal

c d

+ Control-treated Treg + LTRIg-treated Treg

e f g

SVEC4-10 phalloidin staining

MS-1 phalloidin staining

6,000

***

6,000

8,000

6,000

NSNS ***

volume (m3 )

2,000

Basal

volume (m3 )

4,000

Basal

volume (m3 )

4,000

Basal

4,000

2,000

+ +

Lumenal medium Basal medium

Treg LTRIg

0 + + +

Lumenal medium

Basal medium Naive CD4+ T cells

LTRIg

0 + + +

Lumenal medium Basal medium

Treg LTRIg

0 + + +

h i

Lymphatic layers before treg addition

Lymphatic layers after treg addition

Basal protrusion

T cells

5-m pores

Basal

Luminal

Membrane

Basal

Luminal

Membrane

Endothelial cells

Figure 6 | Tregs induce lamellipodia-like structures on the basal surface of lymphatic endothelium. (ad) Representative images of Aza Treg in iSVEC4-10 layers. CFSE Treg in green; phalloidin in red; 63. Yellow scale bar, 28 mm. Yellow arrows indicate thin f-actin protrusions invading the pores of the

membrane and approaching its basal surface as described in the text. (e) Summary of all elds in ad showing volume of phalloidin CFSE negative

structures per eld in mm3. (f) Same as e but with naive non-Treg CD4 Tcells. (g) Same as e with iMS-1 instead of iSVEC4-10. (eg) A total of 2040 elds per condition from 1 experiment representative of 23 experiments. (h,i) Cartoons depicting endothelial protrusion growth displayed in xz plane view.

*Po0.05, **Po0.01, ***Po0.001 by Bonferronis post test of one-way analysis of variance. Error bars are s.e.m. NS, not signicant.

pores of the membrane and approaching its upper/basal surface (Fig. 6a, arrows, and 6h). If iSVEC4-10 were incubated for an additional 4 h after addition of medium to the basal and luminal sides, at f-actin structures with protrusions resembling

lamellipodia spread on the basal surfaces (Fig. 6b,e,i). When measuring these protrusions, we measured the volume of all phalloidin (f-actin ) structures on the basal side of the plastic

membrane. At the start of the experiment, there were no such structures, as there had only been medium on the luminal but not the basal side of the membrane during initial cell growth. These protrusions did not universally resemble lamellipodia, but in the control MOPC21-Treg condition most of the structures that spread from the pores took a broad, at, sheet-like form consistent with lamellipodia. These protrusions were enhanced and more prominent after MOPC21-treated Treg were added to the basal surface (Fig. 6c,e). If Tregs were pretreated with LTbRIg growth of the basal structures was signicantly reduced

(Fig. 6d,e). In contrast, naive non-Treg CD4 T cells induced

less growth of these structures, and that growth was insensitive to LT blockade (Fig. 6f). In contrast to iSVEC4-10, iMS-1 layers failed to grow basal structures, exhibiting growth of only a few rudimentary cytoplasmic protrusions (Fig. 6g).

Basal LEC protrusion growth is regulated by NIK. We hypothesized that the basal structures induced in SVEC4-10 by Treg were involved in promoting Treg transmigration and that their growth would be susceptible to inhibition by the same treatments that prevented Treg transmigration. Specically, inhibition of NIK would reduce growth, while inhibition of JNK or classical NFkB would not. SVEC4-10 pretreated with NIKi demonstrated signicantly less growth of basal structures compared with controls (Fig. 7a). In contrast, pretreatment of iSVEC4-10 layers with inhibitors of JNK or classical NFkB

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Volume basal phalloidin+ SVEC410

15,000

***

10,000

***

5,000

+ +

Lumenal medium Basal medium

Treg LTRIg

JNK-IN-8

NIKi BAY 117082

0 + + +

+ +

Volume basal phalloidin+ SVEC410

Volume basal phalloidin+ MS1

15,000

10,000

** *

5,000

+ +

Lumenal medium

Basal medium Naive CD4+ Tconv

LTRIg

JNK-IN-8

NIKi BAY 11-7082

0 + + +

+ +

15,000

10,000

5,000

+ +

Lumenal medium Basal medium

Treg LTRIg

JNK-IN-8

NIKi BAY 11-7082

0 + + +

Figure 7 | Treg modulation of iSVEC4-10 basal protrusions is mediated by signals via NIK. (ac) Summary volumes of phalloidin CFSE negative

structures per 63 eld 4 h after addition of Aza Treg (a,c) or naive non-

Treg CD4 T cells (b) to iSVEC4-10 (a,b) or iMS-1 (c). A total of 2040

elds per condition from 1 experiment representative of 23 experiments. *Po0.05, **Po0.01, ***Po0.001 by Bonferroni post test of one-way

ANOVA. Error bars are s.e.m. NS, not signicant.

(JNK-IN-8 and BAY 11-7082, respectively), did not alter the growth of basal f-actin structures and in the case of BAY

11-7082, even increased them slightly (Fig. 7a). In agreement with the previous data, the growth of basal structures after the addition of naive non-Treg CD4 T cells was substantially less than that

induced by Treg, and was not modulated by any of the

pharmacological inhibitors (Fig. 7b). Treg did not induce these basal structures when added to inverted MS-1 layers (Fig. 7c) and the pharmacological inhibitors did not alter MS-1 growth. Furthermore, primary LEC basal protrusion growth in response to Treg could be also inhibited by LT blockade or NIKi treatment (Supplementary Fig. 5a). Thus, signals from the LTbR were transduced in SVEC4-10 cells through the non-canonical NFkB pathway via NIK. Induction of this pathway was particular to Treg and did not occur with the BEC cell line MS-1.

The SVEC4-10 basal structures induced by Treg were further characterized by confocal microscopy. Treg were in contact with basal lymphatic protrusions of SVEC4-10 and mouse primary LEC (Supplementary Fig. 5b,c), VCAM-1 was observed in SVEC4-10 basal protrusions near Treg (Supplementary Fig. 5b,c, green arrows), and LTbRIg and NIKi both decreased the mean uorescence intensity of VCAM-1 close to the Treg (Supplementary Fig. 5d). VCAM-1 intensity in the vicinity of CD4 non-Treg did not change with LTbRIg treatment

(Supplementary Fig. 6a). There was no evidence that CCL19 was concentrated in or presented by basal protrusions and no change was observed in CCL19 staining intensity in the vicinity of Treg throughout the lymphatic layers with either LTbRIg or NIKi treatment (Supplementary Fig. 6b). These ndings are consistent with a model in which Treg LTa1b2 engagement with lymphatic

LTbR recruits or induces VCAM-1. Treg responded by decreasing distance and velocity, probably as a result of adhesive interactions, and migrating through the endothelial layer. LEC responded by extending structures that may engage the Treg and by altering the distribution of VCAM-1 with respect to Treg. Thus, there was an active and bidirectional interaction between Treg and LEC.

LT-dependent Treg migration across LEC depends on VCAM-1. To better understand TregLEC interactions, surface molecules expressed by SVEC4-10 were characterized, and both primary mouse skin LEC and SVEC4-10 expressed high levels of LTbR (Fig. 8a). In agreement with our previous work26, SVEC4-10 expressed substantial VCAM-1, but very little ICAM-1, CD62E or CD62P in the steady state (Fig. 8b). Stimulation of SVEC4-10 with the agonistic anti-LTbR mAb

AF.H6 induced a slight increase in VCAM-1 surface expression but did not alter expression of ICAM-1, CD62E or CD62P (Fig. 8b). In keeping with these expression patterns only VCAM-1 blockade reduced Treg transmigration (Fig. 8c). VCAM-1 is a known target of LTbR signalling30. Combined treatment with anti-VCAM-1 mAb plus LTbRIg was not additive (Fig. 8d), suggesting both mediated migration inhibition via a common pathway. In keeping with the in vitro results, VCAM-1 antibody co-injection prevented Treg but not CD4 non-Treg migration

from footpad to draining LN while anti-VLA-4 did not inhibit migration of either Treg or CD4 non-Treg (Fig. 8e). We

developed a simple model of uid ow from the upper to the lower well to more faithfully replicate the in vivo situation. This was accomplished by lling the upper transwell as full as possible while reducing the volume in the lower transwell to the minimum required to cover the bottom of the transwell insert. This resulted in an initial pressure head of B0.80.9 cm H2O that declined during the experiment. This is consistent with estimates of in vivo interstitial pressure gradients from 0.2 to 0.8 cm H2O (ref. 31).

Using this system, LTbRIg, NIKi and anti-VCAM-1 often resulted in greater Treg migration inhibition under ow than in static assays across both iSVEC4-10 (Fig. 8f,g) and primary mouse iLEC (Fig. 8h,i). This may explain in part why the in vivo assays showed greater migration inhibition than did the static in vitro assays. Additional in vitro migration assays were performed using human T cells and primary human LEC with

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uid ow. Mouse LTbRIg was used since it binds human LTa1b2 with an afnity comparable to human LTbRIg (ref. 32). Using four unique donors, LTbRIg inhibited migration of anti-CD3-stimulated human iTreg but not non-Treg CD4 T cells (Fig. 8j). Stimulated human nTreg tended to migrate less when treated

with LTbRIg (Fig. 8j). Three of four nTreg donors showed a reduction in migration when treated with LTbRIg and if the fourth donor were excluded the P value would change from P 0.1 to 0.03, however such exclusion is not easily justiable

(Fig. 8j,k). Given the inherent variability in T-cell samples from

a b

Mouse skin LEC SVEC4-10

100 Negative staining

control

Agonistic anti-LTR

Isotype control for anti-LTR

ICAM-1

VCAM-1 CD62E CD62P

100

% of max

0 10 10 10 10 010 10 10 10 0 10 10 10 10 010 10 10 10 010 10 10 10 010 10 10 10

LTR

c 1.5

Isotype

** d

e WT nTreg footpad migration

Normalized transmigration

*****

WT CD4+ Non-Treg footpad migration

1.0

P=0.51 P=0.6

0.5

P=0.02 P=0.77

% g CFSE+ non-Treg of

all CD4 T cells

1.0

Normalized

transmigration

% CFSE+ Treg of

all CD4 T cells

0.4

0.3

0.2

0.1

0.0

Rat lgG2a

0.5

0.0

Anti-LFA-1 low affinity

Anti-LFA-1 hi/low affinity

Anti-ICAM-1

Anti-VLA-4

+ +

Rat lgG2b Anti-VCAM-1

Anti-VLA-4

Anti-VCAM-1

MOPC21

LTRIg Anti-VCAM-1

+ +

Rat lgG2a

Rat lgG2b Anti-VCAM-1

Anti-VLA-4

f g

iSVEC4-10: static assay

iSVEC4-10: with fluid flow

Human iLEC + human T cells

***

NS ** *

*** **

***

1.00

Normalized transmigration

% Transmigrated

2.0

1.5

1.0

0.5

0.0

1.00

P=0.004 P=0.1

P=0.24

Normalized transmigration

0.75

MOPC21

LTRIg

0.50

27%

21%

32%

34%

0.25

39%

42%

39%

57%

0.25

0.00

MOPC21

LTRIg

NIKi

Anti-VCAM-1

LTRIg + NIKi +anti-VCAM-1

MOPC21

LTRIg

NIKi

Anti-VCAM-1

LTRIg + NIKi +anti-VCAM-1

Non-Treg

iTreg nTreg

h i

iLEC: static assay iLEC: with fluid flow

Human iLEC + human T cells

***

******

***

1.00

Normalized transmigration

* *

1.00

Normalized transmigration

0.75

0.50

34%

31%

15%

54%

35%

28%

36%

42%

0.25

0.00

MOPC21

LTRIg

Anti-VCAM-1

LTRIg + NIKi +anti-VCAM-1

MOPC21

LTRIg

Anti-VCAM-1

LTRIg + NIKi +anti-VCAM-1

NIKi

Non-Treg

iTreg nTreg

MOPC21 LTRIg

l WT nTreg

Linear distance Velocity

Rat IgG2a

Anti-VCAM-1

P< 0.0001

0 50 100 150 200 250 0 5 10 15 20

m min1

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important for DC transmigration across inamed lymphatic endothelium37. There are integrin ligands for VCAM-1 aside from a4 integrins38,39, and non-integrin ligands for VCAM-1 can also regulate transendothelial migration40. Thus, there is precedent for VCAM-1 playing an a4-independent role in lymphatic transmigration. This mechanism also depended on NIK and non-canonical NFkB signalling. While VCAM-1 is typically thought to be regulated via classical NFkB signalling, embryonic broblasts from aly/aly mice, which express a mutated NIK, make VCAM-1 mRNA but fail to express surface VCAM-1 in response to LTbR stimulation, suggesting that NIK regulates VCAM-1 post-translationally41,42. VCAM-1 protein expression has also been shown to be NIK-dependent in response to other stimuli43.

Tregs are among the most substantial populations of T cells to migrate to LN via afferent lymphatics5, and this process is required for optimal Treg-mediated protection of islet allografts2.Therefore, this step could be an important checkpoint in the regulation of inammatory responses in non-lymphoid tissues.Treg proportional representation in inamed skin is less than their representation among cells that reach the draining LN5.This suggests Treg may gain a migratory advantage by inducing increased expression by LEC of molecules conducive to transmigration, or by inducing LEC to engage with and physically promote the movement of Treg across or through lymphatic endothelium. Treg may also impair migration of other cells through lymphatic endothelium2.

LEC and BEC responded differently to LTbR engagement by Treg LTab. Only Treg migration across LEC was impeded by LTbRIg, and only LEC extended basal protrusions in response to engagement of LTbR by Treg LT. Human umbilical vein endothelial cells, BEC and human dermal microvascular endothelial cells, a mixture of LEC and BEC, have been reported to extend microvillus-like projections or docking structures from their apical surfaces around inltrating lymphocytes4446. Others have found similar structures surrounding human DCs interacting with the apical face of LEC47. These structures are microvillus-like projections one to several microns long that form symmetrical rings around transmigrating cells, while the protrusions we observed were tens of microns in width and not restricted to encircling the base of transmigrating Treg. In contrast to these studies4447, we examined interactions of T cells with the basal side. Together these ndings all support a model where endothelial cells play an active role in transendothelial migration by engaging leukocyte via surface receptors and/or other physical engagement.

Tissue to lymphatic migration requires that cells crawl through a three-dimensional interstitial matrix, nd terminal lymphatic

Figure 8 | Treg transmigration through lymphatic endothelium depends on VCAM-1 in vitro and in vivo. (a) SVEC4-10 or mouse skin LEC: grey histograms, isotype control; blue: LTbR. Representative of 3 experiments. (b) SVEC4-10 cultured 48 h, with or without agonistic 1 mg ml 1 anti-LTbR last 24 h, stained for indicated molecules. Isotype control, black histograms; indicated antibody, blue (control-treated) and red (anti-LTbR-treated). Representative of two experiments. (c,d) Aza Treg migrated across iSVEC4-10 to CCL19. SVEC4-10 (anti-ICAM-1 and anti-VCAM-1) or Treg (anti-LFA-1, anti-VLA-4, MOPC21 and LTbRIg) pretreated as indicated. Migration normalized to control. Six transwells from two experiments in c and nine transwells from three experiments in d. (e) Footpad migration. CFSE-labelled WT nTreg or naive CD4 non-Treg co-injected with Rat IgG2a or anti-VCAM-1 or

pretreated with Rat IgG2b or anti-VLA-4. Per cent CFSE cells of popliteal LN CD4 Tcells shown. Treg: 58 mice from 3 experiments; non-Treg: 710 mice

from 3 experiments. (fi) Aza Treg transwell migration across iSVEC4-10 (f,g) and primary mouse iLEC (h,i). Under static conditions (f,h) and with uid ow (g,i). T cells (MOPC21 and LTbRIg) or endothelial cells (NIKi and anti-VCAM-1) treated as indicated. White numerals indicate % decline compared with MOPC21. Migration normalized to MOPC21. Results from nine transwells per condition from three experiments in f and g, and eight transwells from two experiments in h and i. (j,k) Transwell migration of human T cells across human skin iLEC to murine CCL19. T cells treated with MOPC21 or murine LTbRIg. (j) Results by well, 13 per condition from 4 experiments, red bars denote mean. (k) Results by human T-cell donor, control paired with LTbRIg.

(l) Summary of Treg movement in ears during 30 min of image acquisition. In all, 563 Rat IgG2a- and 511 anti-VCAM-1-treated cells tracked using Volocity 6.1.1. Results from 23 ears per condition, 1 20 eld per ear, from 2 experiments. Box and whiskers plot showing minimum, maximum,

mean ( ), median (bar), 25th and 75th percentiles. *Po0.05, **Po0.01, ***Po0.001 by Bonferroni post test of one-way analysis of variance

(c,d,fi), unpaired Students t-test (j,l) and MannWhitney non-parametric test (e). Error bars are s.e.m in cg,h,i. NS, not signicant.

different human donors and the possibility of alloreactivity to the LEC also varying by donor, such variability by donor was not surprising. Finally, we again tracked the movement of live WT nTreg in ear explants. Treg in the vicinity of lymphatics moved farther and faster when co-injected with anti-VCAM-1 but not when co-injected with control Rat IgG2a (Fig. 8l and Supplementary Movies 5 and 6), consistent with the results using LTbRIg (Fig. 3l and Supplementary Movies 1 and 2). In summary Treg depend on LT-LTbR and VCAM-1 interactions with lymphatic endothelium for translymphatic migration under static conditions, and under ow, both in vitro and in vivo, in both mice and humans.

DiscussionWe showed here that among the T-cell subsets examined, Tregs preferentially express and use LTab for migration across lymphatic endothelium in vitro and into afferent lymphatics and migration to LN in vivo. To our knowledge this is the rst demonstration of T-cell LTab playing a direct role in T-cell migration. The nding that LTab is acutely involved in T-cell migration, particularly Treg, is important and may require reinterpretation of results in which LT receptors or ligands were manipulated. The preferential use of LTab by Treg for migration, but not by non-Treg T cells, also suggests that LTab/LTbR interactions may be targets for selectively modulating Treg migration. However, activated helper T cells, Th1 and Th17 cells in particular, have also been described to express LTab (ref. 19), and may use this for migration in other models or circumstances. Further, T-cell LTab has other functions aside from enhancing lymphatic transmigration, for example, enhancing dendritic cell maturation and function33, mast cell activation34 and maintaining broblastic reticular cell structure and function35. It remains to be seen what other functions Treg- and non-Treg-expressed LT might exert. It is also not clear why other cell types, such as naive T cells, do not require LT for lymphatic migration. The answer may have to do with the combination, amount and activation state of integrins and other adhesion molecules, as well as chemokine receptors, that Treg express compared with non-Treg T cells. Treg lymphatic transmigration also depended at least in part on VCAM-1, and blocking data suggested that the roles of LTab/LTbR and

VCAM-1 in transmigration were part of the same pathway. Interestingly, Treg transmigration did not depend on a4 integrins (VLA-4), which are major binding partners for VCAM-1. However, there is evidence that a4 is dispensable for T-cell transmigration across HEV36, and that integrins are not required for lymphatic transmigration of DC11, although VCAM-1 is

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capillaries with assistance from uid ow and chemokine gradients, and traverse a minimal basement membrane. LTbRIg treatment resulted in larger average distances of Treg from lymphatics in ear pinnae. This suggests LTbRIg treatment might have resulted in impaired generation of chemokine gradients. The broblasts that produce the interstitial matrix express LTbR so it is possible that LT-dependent interactions with tissue broblasts were also required for efcient interstitial migration of Treg. Our data suggest that LTab/LTbR interactions may regulate interstitial migration in addition to regulating transmigration across lymphatic endothelium, revealing another unexpected function of the LTab/LTbR axis.

Methods

Animals. C57BL/6 (WT, Lta / , Ltbr / ) and BALB/c mice (814 weeks) were purchased from The Jackson Laboratory (Bar Harbor, ME). Foxp3GFP48 mice on a

C57BL/6 background were from Dr A. Rudensky (Memorial Sloan Kettering Cancer Center). All experiments were performed with age- and sex-matched mice.

Mouse T-cell purication and culture. Mouse LN and spleens were passed through 70-mm nylon mesh (Fisher Scientic) to produce single-cell suspensions and were enriched for CD4 T cells using CD4 negative selection (Stemcell

Technologies). Cells were stained with antibodies to CD4, CD25 and CD44 as detailed in Flow cytometry section. CD4 CD44loCD25 Lta / cells, CD4

CD44loCD25 Foxp3GFP WT cells and CD4 CD25 Lta / cells and CD4 CD25 Foxp3GFP WT cells were sorted using a FACS Aria I (BD Biosciences). Cell purity was 498%. T cell-depleted, 800 rad-irradiated C57BL/6 splenocytes were used as stimulator cells. Puried CD4 CD44loCD25 (or

CD4 CD44loCD25 Foxp3GFP ) T cells (5 104 cells per well) were

cultured with stimulator cells (5 104 cells per well) with IL-2 (20 ng ml 1,

eBioscience), anti-CD3e mAb (1 mg ml 1, clone 145-2C11, eBioscience, 16-0031) for Teff, with the addition of human TGF-b1 (10 ng ml 1, eBioscience) for iTreg, and TGF-b1 and 5-Aza-20-deoxycytidine (10 mM, Sigma-Aldrich) for Aza Treg, in a nal volume of 200 ml of RPMI 1640 supplemented with 10% FBS (Gemini),1 mM sodium pyruvate (Lonza), 2 mM L-glutamine (Lonza), 100 IU ml 1 penicillin (Lonza), 100 mg ml 1 streptomycin (Lonza), 1 non-essential amino

acids (Lonza) and 2 10 5 M 2-ME (Sigma-Aldrich) in U-bottom 96-well plates

(Corning). Cells were cultured for 5 days at 37 C in 5% CO2. Aza Treg culture media replaced at 24 h with medium containing anti-CD3e, IL-2 and TGF-b1. All were given fresh medium with appropriate cytokines but no anti-CD3e mAb at 72 h. CD8 T cells were enriched by magnetic bead negative selection (Stemcell Technologies) then labelled with CFSE for footpad studies, or no CFSE for in vitro migration studies. CD44hiCD4 T cells were enriched for CD4 cells by

magnetic bead negative selection (Stemcell Technologies), and CD44hi T cells were further puried using anti-CD44-PE (1:1,000, clone IM7, eBioscience, 12-0441), anti-PE magnetic microbeads and magnetic separation (Stemcell Technologies).

In vitro suppression assays. CD4 CD25 nTreg and CD4 CD25

non-Treg T cells were sorted from indicated strains. Treg were labelled with CFSE (Life Technologies) and responder non-Treg with eFluor 670 (Molecular Probes, Eugene, OR). Responder CD4 CD25 T cells (5 104 cells per well) were

cultured with or without Treg at responder:Treg ratios of 1:0, 1:1, 2:1, 4:1 and 8:1 with irradiated (800 rad) syngeneic T cell-depleted splenocytes (5 104 cells per

well) in 96-well U-bottom plates (Corning) and anti-CD3e mAb (1 mg ml 1, clone 145-2C11, eBioscience, 16-0031) for 3 days. Cells were collected, and cell division was measured by assessing relative eFluor 670 dilution on an LSRFortessa cytometer (BD).

Cell lines. SVEC4-10 (CRL-2181) and MS-1 (CRL2279) cells were from American Type Culture Collection. Cell lines were expanded after arrival from American Type Culture Collection, veried as mycoplasma-free, were aliquoted and frozen and used until passage 10 after thawing. SVEC4-10 and MS-1 were cultured in DMEM with 4.5 g l 1 glucose (Lonza), containing 10% (v/v) FBS (Benchmark,

Gemini), 2 mM L-glutamine (Lonza), 100 IU ml 1 penicillin (Lonza) and100 mg ml 1 streptomycin (Lonza) in 75-cm2 vented culture asks (Corning) in a horizontal orientation until they reached 7085% conuence, then treated with0.05% Trypsin-EDTA (Gibco) for 57 min at 37 C for detachment, and passaged to new asks at a dilution of 1:6. Cultures were used up to passage ve.

Primary LEC. C57BL/6 mouse primary dermal LECs (C57-6064 L) and human primary dermal LECs (H-6064 L) were from Cell Biologics, Inc. (Chicago, IL), and were cultured according to the manufacturers instructions in manufacturer-provided mouse endothelial cell medium supplemented with 5% FBS, 2 mM L-glutamine, 100 IU ml 1 penicillin, vascular endothelial growth factor, endothelial cell growth supplement, heparin, epidermal growth factor, hydrocortisone or

human endothelial cell medium with 10% FBS, 2% endothelial cell supplement, 2 mM l-glutamine, 100 IU ml 1 penicillin, vascular endothelial growth factor, heparin, epidermal growth factor, broblast growth factor and hydrocortisone.

To split cells, they were incubated with 3.0 ml warm (37 C) 0.25% Trypsin-EDTA solution (Gibco) for 35 min and quenched with 810 ml of Cell Biologics Cell Culture Medium supplemented with 510% FBS as soon as cells had detached. Cells were plated at a 1:2 ratio in fresh asks pre-coated with Gelatin-Based Coating Solution (Cell Biologics) in a humidied, 5% CO2 incubator at 37 C.

Culture media was changed the following day and every 2448 h thereafter. LECs were grown on transwell inserts as described for SVEC4-10, but were seeded at2 105 cells per insert and grown 2 days before use. Inserts for human LEC were

coated with 0.2% gelatin, but mouse LEC required coating for 2 h at 37 C with 10 mg recombinant human laminin 511 (Biolamina AB, Sundbyberg, Sweden) in 100 ml Dulbeccos phosphate-buffered saline to make conuent monolayers.

Human T-cell purication and culture. Naive CD4 T cells (CD4 25 127 CD45RA ) were sorted from non-mobilized peripheral blood apheresis pro

ducts (Memorial Blood Center, St. Paul, MN, USA) using a FACSAria as in ref. 49. These cells were used to produce effectors and induced Treg (iTreg). Both were stimulated with a K562 cell line engineered to express CD86 and the high-afnity Fc Receptor (CD64) (KT86/64)49. KT86/64 were incubated with anti-CD3 (OKT3, Miltenyi, #170-076-116) at 1 mg per 1 106 KT cells per ml for 10 min,

then were washed, irradiated with 10,000 cGray and frozen. The KT86/64 cells were thawed and washed directly before incubation with T cells. OKT3-incubated KT86/ 64 cells were cultured with T cells at a 2:1 Tcell:KT86/64 ratio in the presenceof IL-2 (300 IU ml 1, Novartis). iTregs were expanded in the presence of TGF-b1 (10 ng ml 1, R&D) and rapamycin (109 nM, Rapammune, Wyeth-Ayerst), while control effector cells were cultured without TGF-b1 and rapamycin. nTregs were puried from fresh umbilical cord blood units within 72 h of isolation from the donor (National Placental Blood Program, New York Blood Center) by positive selection using directly conjugated anti-CD25 PE magnetic microbeads (CliniMACS CD25 beads, Miltenyi, #274-01) with AutoMACS or CliniMACS cell separators (Miltenyi, Posseld2 program). CD4CD25 cells (average 64% CD4 )

were cultured with KT86/64 cell lines at a Treg to KT cell ratio of 2:1 (ref. 30). CD25 cells were cultured in X-Vivo-15 (BioWhittaker, Walkersville, MD) or

RPMI 1640 (Invitrogen, Carlsbad, CA) media supplemented with 10% human AB serum (Valley Biomedical, Winchester, VA), L-glutamine (Invitrogen) and N-acetylcysteine (American Regent, Shirley, NY). Recombinant IL-2 (300 IU ml 1,

Novartis) was added on day 3 and maintained for culture duration. Cells were cultured for 1821 days and split every 23 days. nTregs were consistently 485%

CD127 Foxp3 and B70% Foxp3 Helios . iTregs were consistently 6070% CD127 Foxp3 and B10% Foxp3 Helios . Effectors were consistently B10%

CD127-Foxp3 with few to no Foxp3 Helios cells.

Blocking antibodies for in vitro studies. Functional grade-blocking anti-VCAM-1 (clone 429, eBioscience, 16-1061), ICAM-1 (clone YN1, eBioscience, 160541), VLA4/a4 integrin (clone R1-2, eBioscience, 16-0492), high low-afnity

LFA-1/CD11a (clone M17/4, eBioscience, 16-0111) and low-afnity CD11a (clone 2D7, BD, 553118), Rat IgG2a (clone eBR2a, eBioscience, 16-4321) and Rat IgG2b (clone eB149/10H5, eBioscience, 16-4031) were used to treat endothelial layers or T cells at 4 C for 30 min at 10 mg ml 1. In some experiments, low-endotoxin, no-azide (LEAF) grade anti-IL-10 (clone JES5-2A5, Biolegend, 504903), LEAF grade anti-TGF-b1 (clone 19D8, Biolegend, 521703) or LEAF Grade Rat IgG1k (RTK2071, Biolegend, 400413) were incubated with T cells at 10 mg ml 1 for30 min at 4 C before transmigration. T cells were washed 2 before use except in

the case of anti-TGF-b1, anti-IL-10 and control Rat IgG1k, while VCAM-1 and ICAM-1 blockades of endothelial cells were also maintained during assays.

Small-molecule inhibitors and other treatments. Small-molecule inhibitors were used to treat endothelial cell layers. JNK 1 and 2 were blocked with JNK-IN-8 (Calbiochem) at 2 mM for 3 h at 37 C (ref. 50), the classical NFkB pathway was blocked with BAY-11-7082 (Sigma) at 20 mM for 1 h at 37 C (ref. 51), and NIK was blocked with NIKi (Enamine) at 50 mM for 2 h at 37 C (refs 28,29). Cell layers were washed 3 with IMDM 1 insulin/transferrin/selenium (ITS; Invitrogen)

containing 0.5% (w/v) fatty acid-free BSA (Gemini) before use. For in vivo use NIKi was prepared at a stock concentration of 500 mM in dimethylsulphoxide, diluted in PBS/T cell mixtures to a nal volume of 20 ml and injected into the hind footpad at 10 mg kg 1. Murine IL-10 and TGF-b1 were from EBiosciences. For transwell migration in the presence of these cytokines, 10 ng ml 1 each or both together was added to T-cell preparations at the start of the assay.

Transendothelial migration. In all, 1.5 105 MS-1 or SVEC4-10 in 100 ml DMEM

High Glucose (Lonza) 10% FCS (Gemini) on the upper surface of a transwell insert previously coated with 0.2% (w/v) gelatin (Bio-Rad) for 2 h at 37 C. Alternatively, inserts were ipped 180 and cells seeded on lower surface (inverted culture). After 2 days, cell conuency was assessed with a quick haematoxylin and eosin stain (VWR). In some experiments, endothelial cells were pretreated with antibodies, or JNK or NFkB inhibitors and then washed three times with IMDM 1 ITS

containing 0.5% (w/v) fatty acid-free BSA (Gemini), and migration across cell

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ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/ncomms12021

layers was assessed. T-cell subsets were prepared in IMDM 1 ITS containing

0.5% (w/v) fatty acid-free BSA and incubated for 30 min at 37 C with 50 mg ml 1 of LTbRIg (gift from Biogen Idec) or control MOPC21 (BioExCell). A total of 13 105 cells in 100 ml were added to the upper wells of a 24-well transwell

plate with polycarbonate membrane inserts and pore sizes of 5 mm (Corning International). Lower wells contained CCL19 (0.5 mg ml 1), CCL5 (0.5 mg ml 1),

S1P (100 nM), in 600 ml IMDM 1 ITS plus 0.5% (w/v) fatty acid-free BSA. T cells

that migrated to the lower well after 4 h at 37 C were counted in four to eight full 1-mm corner squares with a haemocytometer. Migration with uid ow was performed as described for static assays with the following modication: upper wells were lled with 340 ml medium and the lower wells with 360 ml. This resulted in an initial static pressure head of B0.80.9 mm H2O.

Time-lapse microscopy. CFSE T cells (1 104 cells per transwell) migrating

across endothelial monolayers to CCL19 (0.5 mg ml 1) were visualized by uorescence microscopy (Axiovert 200M) with a 20 objective. One image was

captured every minute for 30 min with Axiovision 4.7.1 software (Carl Zeiss Jena GmbH). Mean distance from origin and mean velocity analysed with Volocity version 6.1.1 software (Perkin Elmer).

Conventional and confocal microscopy. CFSE-stained T cells (5 104 cells per

transwell) migrated for 4 h across endothelial monolayers to CCL19 (0.5 mg ml 1), xed for 20 min at 4 C with 4% (w/v) paraformaldehyde (Affymetrix), and paraformaldehyde then neutralized for 10 min at 4 C with PBS 0.1 M glycine (Sigma-Aldrich), pH 7.4. Cells were permeablized with PBS 0.2% (v/v) Triton X-100 (Sigma-Aldrich), stained for 30 min at 4 C with Alexa uor 555-phalloidin (Life Technologies) and washed with PBS containing 0.2% (v/v) Triton X-100 followed by PBS alone. In some experiments cell layers were also stained with polyclonal goat anti-VCAM-1 at 2 mg ml 1 (sc-1504, Santa Cruz) and detected with Donkey anti-goat Alexa Fluor 647 at 5 mg ml 1 (Jackson Immunoresearch, 705-606-147). Transwell membranes were transferred onto glass slides and visualized by confocal microscopy (Zeiss LSM 510 Meta, and LSM5 Duo) with a

63 or 40 objective, respectively. Z-stack images were acquired every 1 mm with

2-mm slice thicknesses for examination of in vitro endothelial monolayers. Images were analysed with Volocity version 6.1.1 software (Perkin Elmer).

Ex vivo live imaging. C57BL/6 recipient ears were depilated with Nair Lotion Hair Removal for 1 min, and loose hair and depilatory solution were washed with several saline rinses. Three days later mice were anaesthetized with ketamine/xylazine and given 1 mg azide-free Alexa-488-labelled anti-LYVE-1 in 10 ml PBS (clone ALY7, eBioscience, Custom Product) into the ear pinna to identify lymphatic vessels as previously shown52. Six hours later, 1 106 magnetic bead-puried euor 670

(eBioscience), CD25 nTreg derived from 57 C57BL/6 donor LN and spleens or

68 Lta / donor spleens per experiment were injected into the ear pinna in 10 ml. Treg were treated with LTbRIg or MOPC21 as indicated for 30 min at 37 C and then washed 2 with PBS. In some experiments 5 mg of no-azide, low

endotoxin (NA/LE) grade anti-VCAM-1 (429, BD) or NA/LE Rat IgG2a isotype control (BD) were co-injected with T cells. Four hours after T-cell injection mice were killed, ears removed, secured to a Sylgard 184 (Dow Corning) silicone block with surgical tape and covered with HEPES buffered saline at 37 C for imaging. Movies were recorded beginning no later than 30 min after ear removal. Z-stacks (3-mm slices, 3-mm interval, 1824-mm stack height) were collected once per minute for 30 min with a Zeiss 710 upright microscope in confocal mode using a

20 water immersion objective. Movies were analysed with Volocity version 6.1.1 software (Perkin Elmer).

Flow cytometry. Mouse LN and spleen were passed through 70-mm nylon mesh screens (Fisher Scientic), to produce single-cell suspensions. Cell suspensions were treated with anti-CD16/32 (clone 93, eBioscience) to block Fc receptors, then stained with antibodies to surface molecules and washed two times in FACS buffer (PBS with 0.5% w/v BSA). Flow cytometry antibody clones, uorochromes, concentrations, vendors and catalogue numbers for mouse cells (Supplementary Table 1) and human cells (Supplementary Table 2) can be found in the Supplementary Materials online. For preparation of single-cell suspensions from islet grafts, grafts were exposed by partially peeling the kidney capsule from the kidney surface, removed, digested with collagenase P (1.5 mg ml 1) at 37 C in a water bath for 5 min, and then made into a single-cell suspension by passing through 70-mm nylon mesh screens (Fisher Scientic). For detection of LT by ow cytometry, cells were incubated with MOPC21 or LTbRIg at 2 mg ml 1 for 60 min at 37 C in HBSS (Lonza), 2% FBS (Gemini), 1 mM sodium pyruvate (Lonza), 2 mM L-glutamine (Lonza), 100 IU ml 1 penicillin (Lonza), 100 mg ml 1 streptomycin (Lonza), 1 non-essential amino acids (Lonza) at no more than

5 106 cells per ml. Cells were washed twice in the HBSS solution, then stained

for 45 min with surface antibodies including Rat anti-Mouse IgG1k at 200 ng ml 1 for 45 min at 4 C. Cells were then washed twice in HBSS buffer, and were xed with 4% paraformaldehyde at room temperature in the dark for 10 min, washed, run on an LSR Fortessa ow cytometer (BD Biosciences), and results analysed with FlowJo 8.7 (Treestar).

When appropriate, intracellular staining for Foxp3 followed the extracellular step using eBiosciences Foxp3 intracellular staining buffer. ef450 xable viability dye (eBiosciences) was used according to the manufacturers protocol. Cells were then xed, processed and analysed as above. For SVEC4-10 ow cytometry 2 104

SVEC4-10 cells were cultured in a 24-well plate overnight, medium was removed and replaced with 0.5 ml medium containing agonistic anti-LTbR (AFH6 1 mg ml 1, Biogen Idec) or control mAb (Ha4/8 1 mg ml 1, BD, 553961)

for 24 h. At that time cells were collected for ow cytometry.

Real-time PCR. Primer sequences were: Foxp3 forward 50-CCCAGGAAAGAC AGCAACCTT-30 and reverse 50-TTCTCACAACCAGGCCACTTG-30; Ltbr forward 50-TTGCTGGGACCAGGACAAGGAATA-30 and reverse 50-GGCTGCAT ACCGCAAAGACAAACT-30; Ccr7 forward 50-CACGCTGAGATGCTCACTG G-30 and reverse 50-CCATCTGGGCCACTTGGA-30; S1pr1 forward 50-GTGT AGACCCAGAGTCCTGCG-30 and reverse 50-AGCTTTTCCTTGGCTGGAGA G-30; Il10 forward 50-AGTGCCTGGTTGCTGCTTTACC-30 and reverse 50-AG CCGTAGGGTCTTTAGATTTTCAC-30; Tgfb1 forward 50-CTGCTGAGGCTC AAGTTAAAAGTG-30 and reverse 50-CAGCCGGTTGCTGAGGTAG-30; Prf1 forward 50-GAGAAGACCTATCAGGACCA-30 and reverse 50-AGCCTGTGGT AAGCATG-30; Gzmb forward 50-CCTCCTGCTACTGCTGAC-30 and reverse 50-GTCAGCACAAAGTCCTCTC-30.

Ifng forward 50-CTTTGGACCCTCTGACTTGAG-30 and reverse 50-TCAA TGACTGTGCCGTGG-30; Bcl2 forward 50-CTGGCATCTTCTCCTTCCAG-30 and reverse GACGGTAGCGACGAGAGAAG-30; Bcl2l11(Bim) 50-GAGATACGGA TTGCACAGGA-30 and revserse 50-TCAGCCTCGCGGTAATCATT-30; Ppia (Cyclophilin A) forward 50-AGGGTGGTGACTTTACACGC-30 and reverse 50-A TCCAGCCATTCAGTCTTGG-30; Cd39 forward 50-CGAGAAGGAGAATGAC ACAGG-30 and reverse 50-ATGTTGGTATCAGTTCGGTGG-30; Cd73 forward 50-GCTTCAGGGAATGCAACATG-30 and reverse 50-TGCCACCTCCGTT TACAATG-30; Ctla4 forward 50-CATGCCCGGATTCTGACTTC-30 and reverse 50-GGCATTTTCACATAGACCCCTG-30; Tnfrsf14/Hvem forward 50-TGAAGCA GGTCTGCAGTGAGCATA-30 and reverse 50-GCCATTTGCATGGGCGGTA TATGT-30; Lta forward 50-TGAAACCTGCTGCTCACCTTGTTG-30 and reverse 50- AAGAGAAGCCATGTCGGAGAAAGG-30; Ltb forward 50-TCCAATGCTT CCAGGAATCTAGCC-30 and reverse 50-GACAGGATGGTCATCCATGCAGA T-30; and Tnfsf14/LIGHT forward 50- AAAGGCTCCTGGGAGAAGCTGATA-30 and reverse 50-ATACCTATCAAGCTGGCGTTGGCT-30. Total cellular RNA was extracted from the indicated T cell and endothelial cell populations using Trizol reagent (Invitrogen), digested with RNase-free DNase I to remove any contaminating genomic material (Invitrogen), and reverse-transcribed into cDNA using Omniscript RT Kit (Qiagen) and random primers (Invitrogen) according to the manufacturers protocol. mRNA expression levels were quantied by real-time PCR using Quantitect SYBR Green PCR kit (Qiagen) with an ABI Prism 7900HT (Applied Biosystems). PCR consisted of a 15-min 95 C denaturation step followed by 45 cycles of 15 s at 94 C, 20 s at 56 C and 20 s at 72 C. Values for specic gene mRNA expression as a % of cyclophilin A were calculated as: 2(Ct of Cyclophilin A Ct specic gene) 100.

Murine islet isolation and transplantation. The following procedure has been adapted from a published protocol53.

Reagents. EuroCollins solution was prepared by adding 20 ml electrolyte additive solution (Catalogue No: 99-409-CI, Cellgro) to 1 l Glucose Solution (Catalogue No: 99-408-CM, Cellgro). Ficoll 400-DL, (Catalogue No. F9378, Sigma) solutions were prepared at concentrations of 25, 23, 21 and 11% (w/v) in EuroCollins solution. Lower concentrations of Ficoll solution were diluted from 25% stock. Collagenase P (Catalogue No. 11213873001, Roche) solution was prepared at 1.5 mg ml 1 in HBSS with Ca2 and Mg2 (Catalogue No.

12-020-CV, Cellgro), with 2 mg ml 1 BSA (Catalogue No. A7906, Sigma). RPMI 1640 (Catalogue No. 12-702F, Lonza) with 10% FBS (Benchmark, Gemini) was also prepared.

Injection. Just before dissection, BALB/c mice were killed by CO2 inhalation followed by cervical dislocation. The entire mouse was with 70% ethanol and a V-shaped incision was made starting at the genital area and ending near the ribcage. Mice were oriented with the tail facing away from the experimenter and intestines were moved aside to expose the pancreas and common bile duct. A haemostat clamp was placed on the duodenum just posterior of the ampulla, where the bile duct drains, leaving a small space for collagenase P solution to enter the duodenum.

A 30-g needle on a 3-ml syringe containing 3 ml of cold collagenase P solution was prepared, and the needle inserted into the common bile duct at the joint site of the hepatic duct and the cystic duct. The pancreas was then inated by slowly but steadily injecting the solution. Next, the pancreas was removed and placed in a 50-ml tube containing 2 ml of collagenase P solution on ice. With a maximum of2.53 pancreata per 50-ml tube. This step was done as quickly as possible. Digestion. Note: Islets stick to plastic, so all plastic surfaces that will contact

islets must be pre-coated with RPMI 1640/10% FBS!

The 50-ml tubes with pancreata were placed in 37 C water bath for 1315 min. After incubation, tubes were shaken by hand to disrupt the pancreas until the suspension was homogeneous. Next, cold RPMI 1640/10% FBS was added to the tube to bring the total volume to 50 ml. Tubes were centrifuged at 70g for 1 min and the supernatant was aspirated leaving B5 ml to avoid disturbing the pellet.

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NATURE COMMUNICATIONS | DOI: 10.1038/ncomms12021 ARTICLE

A Cellector Tissue Sieve (Bellco Glass) was placed in a funnel over a 50-ml conical tube and the sieve was wetted with 20 ml of RPMI 1640/10% FBS. The pellet was then re-suspended in 20 ml RPMI 1640/10% FBS and poured through sieve into a 50-ml tube and the screen was washed with 10 ml RPMI 1640/10% FBS. Tubes were then centrifuged at 90g for 1 min. Supernatant was aspirated the pellet was re-suspended by tapping with the hand, then RPMI 1640/10% FBS was added up to 50 ml. Pellets were washed in this manner two more times. Finally, the supernatant was aspirated as completely as possible. (as remaining buffer might cause the change of the density of the Ficoll.)

Ficoll gradient enrichment. Perform this process quickly. Long-term Ficoll exposure is toxic to islets.

The 50-ml tubes were tapped on the bottom by hand to ensure pellet was completely dissociated before adding Ficoll as unbroken tissue is difcult to re-suspend in dense, viscous Ficoll. Pellet was then re-suspended in 10 ml of Ficoll 25% by nger tapping. Remaining Ficoll densities were then layered on top using a 5-ml pipette in this order: 23, 21 and 11%. Ficoll gradient preparations were centrifuged at 600g at 4 C, with no brake for 15 min. If centrifuge allows control over acceleration rate, set in the middle of the range. Islets were picked from the interface of the second and third layers using a bulb pipette and transferred to a 50-ml tube containing B25 ml of cold RPMI 1640/10% FBS. Islet pellets were washed twice, centrifuging at 270g for 4 min and 188g for 3 min using the brake. Islet pellets were then re-suspended in 20 ml RPMI 1640/10% FBS.

Islet picking. Islets were transferred islets to a 100-mm Petri dish and were hand-picked using an inverted light microscope and a 200 ml pipette, and transferred into a 1.7-ml Eppendorf tube and kept on ice until ready for transplantation.

Transplantation. A total of 400 freshly isolated islets were hand-picked and transplanted beneath the renal capsule of C57BL/6 recipients made diabetic (blood glucose 4300 mg dl 1) by intraperitoneal injection of 180 mg kg 1 streptozotocin (Sigma-Aldrich) at least 1 week before transplant. nTregs were puried with anti-CD25 PE magnetic bead positive selection (Miltenyi) from C57BL/6 donor LN and spleens or Lta / donor spleens. Cells were then labelled with euor 670

at 5 mM (eBioscience) or CFSE at 5 mM (Invitrogen) according to the manufacturer instructions, and 1 106 magnetic bead-puried CD25 nTreg were injected

either with islets under the kidney capsule (local transfer) or i.v. Blood glucose o150 mg dl 1 after transplantation was considered engraftment, and4300 mg dl 1 was considered diabetic or graft rejection as appropriate. Blood glucose was monitored via tail nicking with a handheld meter (Therasense).

Footpad and whole-mount ear migration assays. CD4 T cells were enriched

by magnetic bead negative selection (Stemcell Technologies). CD25hi Tregs were further puried using anti-CD25-PE, anti-PE microbeads and magnetic separation (Stemcell Technologies), then labelled with CFSE (Life Technologies). T cells were treated with LTbRIg or MOPC21 at 50 mg ml 1 as indicated for 30 min at 37 C and then washed 2 with PBS. In some experiments 2.5 mg of NA/LE grade

anti-VCAM-1 (429, BD, 553329) or NA/LE Rat IgG2a isotype control (clone R35-95 BD, 554687) were co-injected with T cells. In other experiments T cells were pretreated with NA/LE grade anti-VLA-4 (clone R1-2, BD, 553153), NA/LE grade Rat IgG2b (clone R35-38, BD,555845) at 10 mg ml 1 for 30 min at 4 C and washed 3 with PBS before injection. Mice were anaesthetized and 1 106

CD25 Tregs or CD4 non-Tregs were injected into the footpads or ear pinnae

in 20 ml PBS. For footpad assays, draining popliteal LN were collected 12 h post injection and processed for ow cytometry. For ear pinnae assays, whole-mount ears were collected 4 h post injection, xed for 10 min at room temperature with 4% paraformaldehyde, then stained with anti-LYVE-1 at 5 mg ml 1 (Fitzgerald, 70R-LR003), anti-CCL21 at 5 mg ml 1 (R&D Systems, AF457) in PBS 0.5% BSA0.3% Triton X-100 overnight at 4 C, washed with PBS 0.5% BSA 0.3% Triton X-100, stained with Donkey anti-Rabbit Cy3 at 5 mg ml 1 (Jackson

ImmunoResearch, 711-165-152) and Donkey anti-Goat Alexa Fluor 488 at5 mg ml 1 (Jackson ImmunoResearch, 705-546-147) for 2 h at room temperature, washed 2 in PBS 0.5% BSA 0.3% Triton X-100, xed once more, transferred to

glass slides and mounted with Prolong Gold (Life Technologies).

LN egress assay. We adapted a procedure previously reported to determine T-cell egress rates from LNs54. A mixture of 5 106 WT C57BL/6 eFluor 670 CD4

T cells and 5 106 CFSE Lta / CD4 T cells was injected i.v. into naive

C57BL/6 mice. After 18 h 100 mg anti-CD62L (MEL-14, BioXCell) or control Rat IgG2a (2A3, BioXCell) was injected i.v. After 18 h of antibody injection LN and spleen were collected and analysed by ow cytometry.

Statistical analysis. No statistical tests were used to predetermine the size of experiments. Sample and experiment sizes were determined empirically for sufcient statistical power. No samples were excluded specically from analysis, and no randomization or blinding protocol was used. Survival curves were analysed according to the KaplanMeier estimator, and the difference between two groups was determined by the log-rank (MantelCox) test. Log-rank, two-tailed Students t-tests, MannWhitney non-parametric tests, one-way analysis of variance with Tukey, Bonferroni post tests or KruskalWallis test with Dunns post tests were performed using Graphpad software (Prism V5.0). MannWhitney and

KruskalWallis tests were used when data were non-Gaussian due to presence of outliers or data clustered at a limit of detection. For multiple comparisons, Tukey post tests were used when all possible comparisons were logically meaningful, and Bonferroni or Dunns post tests were used when only certain pairs of comparisons were experimentally or logically possible. A P value of o0.05 was considered signicant for one-way analysis of variance and t-tests, and for multiple comparisons signicance level was adjusted for the number of comparisons being made by the software. Statistical analysis was performed on groups with similar variance. Error bars in gures and value ranges given in the text are means.e.m. unless otherwise noted.

Image adjustments. Images were adjusted for contrast and brightness over the entire eld to improve visibility.

Study approval. All animal experiments were performed in accordance with Institutional Animal Care and Use Committee approved protocols.

Data availability. The data that support the ndings of this study are available from the corresponding author on request.

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Acknowledgements

This work was supported by PHS RO1 AI062765 to J.S.B., grants from the American Society of Transplant Surgeons and the Maryland Living Legacy Foundation to C.C.B., PHS T32 CA154274 to S.A., R01 HL11879 and RO1 CA72669 to B.R.B. and P01 CA 065493 to B.R.B. and K.L.H. Flow sorting was performed at the University of Maryland Marlene and Stewart Greenebaum Cancer Center Flow Cytometry Shared Service by Dr Ferenc Livak and Karen Underwood. We thank Jeff Browning (Biogen Idec) for LTbRIg and stimulatory anti-LTbR clone AF.H6. We are grateful for technical support from Dan Chen, Juanjuan Zhao, Usha Rai and Chelsea Wagner.

Author contributions

C.C.B. and J.S.B. wrote the manuscript, and planned, and analysed the experiments; D.I. performed islet transplants and local Treg co-transfers; Y.X. performed ex vivo phenotypic analysis of WT and Lta / Treg, Treg suppression assays; and Y.X. and S.A.

performed and analysed static ear pinna Treg migration experiments; M.H. performed some in vitro studies of iSVEC4-10 protrusion growth; T.S. sectioned and stained LN for imaging studies and provided guidance in planning and analysis of experiments; K.L.H. and B.R.B. generated human T-cell subsets and provided experimental design and interpretive guidance; C.C.B. performed all other experiments not otherwise noted.

All authors edited and approved the manuscript.

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How to cite this article: Brinkman, C. C. et al. Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration. Nat. Commun. 7:12021 doi: 10.1038/ncomms12021 (2016).

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Regulatory T cells (Tregs) are essential to suppress unwanted immunity or inflammation. After islet allo-transplant Tregs must migrate from blood to allograft, then via afferent lymphatics to draining LN to protect allografts. Here we show that Tregs but not non-Treg T cells use lymphotoxin (LT) during migration from allograft to draining LN, and that LT deficiency or blockade prevents normal migration and allograft protection. Treg LTαβ rapidly modulates cytoskeletal and membrane structure of lymphatic endothelial cells; dependent on VCAM-1 and non-canonical NFκB signalling via LTβR. These results demonstrate a form of T-cell migration used only by Treg in tissues that serves an important role in their suppressive function and is a unique therapeutic focus for modulating suppression.

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Title

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration

Author

Brinkman, C Colin; Iwami, Daiki; Hritzo, Molly K; Xiong, Yanbao; Ahmad, Sarwat; Simon, Thomas; Hippen, Keli L; Blazar, Bruce R; Bromberg, Jonathan S

Pages

12021

Publication year

2016

Publication date

Jun 2016

Publisher

Nature Publishing Group

e-ISSN

20411723

Source type

Scholarly Journal

Language of publication

English

DOI

https://doi.org/10.1038/ncomms12021

ProQuest document ID

1798283645

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration

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