Abstract

Detection of mutations in cancer is particularly important in terms of proper treatment and targeted therapy. The aim of this study was the comparison of two methods: allele-specific PCR (AS-PCR) and dideoxysequencing applied for the identification of [BRAF] gene mutations in wild-type gastrointestinal stromal tumors (WT GISTs). We have optimized the conditions for the detection V600E mutation representing the c.1799 T>A substitution by AS-PCR and have used dideoxysequencing to verify our results. In nine cases, we were able to detect the mutation by AS-PCR approach; however, the mutations have been confirmed by dideoxysequencing in four cases only. AS-PCR is fast and low cost method for the detection of V600E mutation which was validated as a sensitive assay for the identification of the most common [BRAF] mutation in DNA extracted from paraffin-embedded tissue of WT GISTs.

Details

Title
Limits of Dideoxysequencing in the Detection of Somatic Mutations in Gastrointestinal Stromal Tumors
Author
Jasek, K; Buzalkova, V; Szepe, P; Plank, L; Lasabova, Z
Pages
13-20
Publication year
2015
Publication date
2015
Publisher
De Gruyter Poland
ISSN
13358421
e-ISSN
13384139
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1860845024
Copyright
Copyright De Gruyter Open Sp. z o.o. 2015