A Superoxide Dismutase Capable of Functioning with Iron or Manganese Promotes the Resistance of Staphylococcus aureus to Calprotectin and Nutritional Immunity

Abstract

Staphylococcus aureus is a devastating mammalian pathogen for which the development of new therapeutic approaches is urgently needed due to the prevalence of antibiotic resistance. During infection pathogens must overcome the dual threats of host-imposed manganese starvation, termed nutritional immunity, and the oxidative burst of immune cells. These defenses function synergistically, as host-imposed manganese starvation reduces activity of the manganese-dependent enzyme superoxide dismutase (SOD). S. aureus expresses two SODs, denoted SodA and SodM. While all staphylococci possess SodA, SodM is unique to S. aureus, but the advantage that S. aureus gains by expressing two apparently manganese-dependent SODs is unknown. Surprisingly, loss of both SODs renders S. aureus more sensitive to host-imposed manganese starvation, suggesting a role for these proteins in overcoming nutritional immunity. In this study, we have elucidated the respective contributions of SodA and SodM to resisting oxidative stress and nutritional immunity. These analyses revealed that SodA is important for resisting oxidative stress and for disease development when manganese is abundant, while SodM is important under manganese-deplete conditions. In vitro analysis demonstrated that SodA is strictly manganese-dependent whereas SodM is in fact cambialistic, possessing equal enzymatic activity when loaded with manganese or iron. Cumulatively, these studies provide a mechanistic rationale for the acquisition of a second superoxide dismutase by S. aureus and demonstrate an important contribution of cambialistic SODs to bacterial pathogenesis. Furthermore, they also suggest a new mechanism for resisting manganese starvation, namely populating manganese-utilizing enzymes with iron.

About the Authors:

Yuritzi M. Garcia

Affiliation: Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, IL, United States of America

ORCID http://orcid.org/0000-0002-3858-4684

Anna Barwinska-Sendra

Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom

ORCID http://orcid.org/0000-0002-9342-2805

Emma Tarrant

Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom

Eric P. Skaar

Affiliation: Department of Pathology Microbiology and Immunology, Vanderbilt University Medical Center Nashville TN, United States of America

Kevin J. Waldron

* E-mail: [email protected] (TEKF); [email protected] (KJW)

Affiliation: Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom

ORCID http://orcid.org/0000-0002-5577-7357

Thomas E. Kehl-Fie

* E-mail: [email protected] (TEKF); [email protected] (KJW)

Affiliation: Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, IL, United States of America

ORCID http://orcid.org/0000-0001-8234-1113Abstract

Staphylococcus aureus is a devastating mammalian pathogen for which the development of new therapeutic approaches is urgently needed due to the prevalence of antibiotic resistance. During infection pathogens must overcome the dual threats of host-imposed manganese starvation, termed nutritional immunity, and the oxidative burst of immune cells. These defenses function synergistically, as host-imposed manganese starvation reduces activity of the manganese-dependent enzyme superoxide dismutase (SOD). S. aureus expresses two SODs, denoted SodA and SodM. While all staphylococci possess SodA, SodM is unique to S. aureus, but the advantage that S. aureus gains by expressing two apparently manganese-dependent SODs is unknown. Surprisingly, loss of both SODs renders S. aureus more sensitive to host-imposed manganese starvation, suggesting a role for these proteins in overcoming nutritional immunity. In this study, we have elucidated the respective contributions of SodA and SodM to resisting oxidative stress and nutritional immunity. These analyses revealed that SodA is important for resisting oxidative stress and for disease development when manganese is abundant, while SodM is important under manganese-deplete conditions. In vitro analysis demonstrated that SodA is strictly manganese-dependent whereas SodM is in fact cambialistic, possessing equal enzymatic activity when loaded with manganese or iron. Cumulatively, these studies provide a mechanistic rationale for the acquisition of a second superoxide dismutase by S. aureus and demonstrate an important contribution of cambialistic SODs to bacterial pathogenesis. Furthermore, they also suggest a new mechanism for resisting manganese starvation, namely populating manganese-utilizing enzymes with iron.

Author Summary

During infection, pathogens must overcome the restriction of essential nutrients such as manganese by the host, while simultaneously coping with other host defenses such as the oxidative burst. Using the host protein that limits manganese availability during infection and mice lacking this effector, we determined that acquisition of a second superoxide dismutase that is capable of using either manganese or iron enhances the ability of Staphylococcus aureus to cause infection. When manganese-starved by the host, this cambialistic enzyme enables S. aureus to maintain superoxide dismutase activity and survive when exposed to oxidative stress. These results reveal the important contribution of cambialistic superoxide dismutases to bacterial pathogenesis and represent a new mechanism for resisting manganese starvation during infection.

Citation: Garcia YM, Barwinska-Sendra A, Tarrant E, Skaar EP, Waldron KJ, Kehl-Fie TE (2017) A Superoxide Dismutase Capable of Functioning with Iron or Manganese Promotes the Resistance of Staphylococcus aureus to Calprotectin and Nutritional Immunity. PLoS Pathog 13(1): e1006125. doi:10.1371/journal.ppat.1006125

Editor: Andreas Peschel, University of Tubingen, GERMANY

Received: October 14, 2016; Accepted: December 14, 2016; Published: January 19, 2017

Copyright: © 2017 Garcia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: This work was supported by operating grants for the National Institutes of Health to TEKF (K22 AI104805 and R01 AI118880) and EPS (R01 AI101171), a Royal Society and Wellcome Trust-funded Sir Henry Dale fellowship to KJW and ET (098375/Z/12/Z), a BBSRC Ph.D. studentship to ABS, and a Basil O'Connor Starter Scholar Award from March of Dimes to TEKF. The contents of this work are solely the responsibilities of the authors and do not reflect to official views of the National Institutes of Health or the March of Dimes. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

The spread of antibiotic resistance amongst bacteria has led both the Centers for Disease Control and Prevention and the World Health Organization to state that infections represent a serious threat to human health [1, 2]. This threat is exemplified by Staphylococcus aureus, a Gram-positive bacterium that asymptomatically colonizes one third of the population and is a leading cause of antibiotic-resistant infections [3-5]. A promising area of investigation is elucidating how pathogens overcome host defenses such as the active withholding of essential nutrients and the oxidative burst of immune cells.

During infection, pathogens must obtain all of their nutrients from the host, including the essential metal ions that are needed for the approximately one-third of all bacterial proteins that require a metal cofactor [6-8]. This requirement is exploited by the host, which restricts the availability of these essential nutrients, a defense termed nutritional immunity [9-13]. The canonical example of nutritional immunity is the iron (Fe)-withholding response [10, 11]. In addition to Fe, the host also restricts the availability of manganese (Mn) and zinc (Zn) [9, 12-14]. The prototypical example of Mn and Zn restriction is the staphylococcal abscess, which is rendered virtually free of these metals during infection [9, 14]. A critical component of the Mn- and Zn-withholding response is the host protein calprotectin (CP) [9, 12, 14]. This innate immune effector is highly expressed in neutrophils in which it comprises 40-60% of the cytoplasmic protein, and at sites of infection it can be found in excess of 1 mg/ml [15, 16]. Loss of CP results in host defects in metal sequestration and increased sensitivity to a number of bacterial and fungal pathogens, including S. aureus [9, 14, 17-19]. In culture, CP inhibits the growth of a similarly wide range of pathogens [16-20]. The antimicrobial activity of CP is dependent on binding of metal ions to its two transition metal-binding sites [14, 20, 21]. The first site or 'Mn/Zn site' is comprised of six histidines and is capable of binding either Mn or Zn with nanomolar and picomolar affinities (Kd), respectively [14, 20-22]. The second site or 'Zn site' is comprised of three histidines and an aspartic acid and binds Zn with picomolar or sub-picomolar affinity [14, 20, 22].

In order to cause disease, invading pathogens must not only overcome nutrient starvation but also simultaneously cope with other host defenses, such as the oxidative burst of neutrophils and other immune cells [23]. Bacteria defend themselves from the oxidative burst by numerous mechanisms, including enzymes such as superoxide dismutases (SODs) that detoxify the damaging reactive oxygen species with which they are bombarded [24-27]. The activating metal cofactor divides the SOD enzymes into several families, with the most common amongst bacteria belonging to a single protein superfamily, which utilizes either Mn or Fe as cofactor. It has proven exceptionally difficult to predict which metal is utilized by a given Mn/Fe-dependent SOD [24, 28], in part due to the fact that Fe- and Mn-SODs coordinate their metal cofactor using the same protein ligands within an identical protein fold [28, 29]. This structural similarity also enables both Fe- and Mn-SODs to bind the other, non-cognate metal, but this usually results in an inactive enzyme; most members of this protein family are strictly dependent on their cognate metal for catalysis [28-31]. Notably, a subset of the Mn/Fe-dependent SOD family is active when loaded with either Fe or Mn [32-40]. While these 'cambialistic' SODs are present in a diverse group of microbes, analysis of their activity has largely been limited to in vitro studies, limiting our understanding of how cambialism benefits microbes and the contribution of these enzymes to colonization of the host.

S. aureus possesses two SODs, SodA and SodM, both of which are cytoplasmic and are reported to be Mn-dependent [41-43]. While all staphylococci possess SodA, SodM is unique to S. aureus [44]. Highlighting their importance to virulence, loss of either SodA or SodM in a skin model of infection or loss of both SODs in a systemic mouse model, reduces the ability of S. aureus to cause disease [14, 42]. However, a molecular explanation for the advantage that S. aureus gains by expressing two apparently Mn-dependent SODs is unknown. Host-imposed Mn starvation mediated by CP reduces total staphylococcal SOD activity, both in culture and during infection, which renders S. aureus more sensitive to oxidative stress and neutrophil-mediated killing [13, 14, 20]. Yet paradoxically, the simultaneous loss of both SodA and SodM renders S. aureus more sensitive to CP [14], indicating that SodA and/or SodM somehow enhance the ability of S. aureus to resist metal starvation.

Given the importance of the two staphylococcal SODs to infection, we have elucidated their respective contributions to resisting oxidative stress and nutritional immunity. This analysis revealed that SodA is important for resisting oxidative stress and infection when Mn is abundant, whereas SodM is important under Mn-deplete conditions. Our data demonstrate that SodM is in fact cambialistic, possessing equal enzymatic activity when loaded with Mn or Fe. We propose that the ability of SodM to utilize Fe enables S. aureus to retain SOD activity when starved of Mn by the host, thereby enhancing the ability of the bacterium to overcome nutritional immunity, resist oxidative stress, and ultimately cause infection.

Results

Mn availability differentially regulates the expression of the S. aureus SODs

Several prior studies have examined the impact of Mn availability on the expression of SodA and SodM; however, different conclusions were reached [41, 42, 45]. In light of this ambiguity, we initially assessed the impact that CP and oxidative stress have on sodA and sodM expression. When normalized to optical density in order to account for differences in growth, high levels of sodA transcription were observed regardless of whether CP was present (Fig 1A), whereas the presence of CP enhanced sodM expression independent of the presence of oxidative stress (Fig 1B). These results indicate that sodM, but not sodA, is induced in response to Mn or Zn limitation. To clarify which metal gave rise to this effect, we used mutant CP variants that lack either the Mn/Zn site ([delta]Mn/Zn site mutant, which does not bind Mn) or the Zn site ([delta]Zn site mutant, which binds both Mn and Zn) [20]. As expected, neither mutant induced the expression of sodA (Fig 1C). The increased expression of sodM observed with wild type (WT) CP is lost in the presence of the [delta]Mn/Zn site mutant, but not the [delta]Zn site mutant, indicating that sodM is induced in response to Mn limitation (Fig 1D).

Download:

*

PPT

PowerPoint slide

*

PNG

larger image

*

TIFF

original image

Fig 1. Expression of sodA and sodM varies by Mn availability.

S. aureus carrying (A, C, & E) pSodA and (B, D, & F) pSodM YFP reporter constructs were grown in the presence of either wild type CP (A-F), the [delta]Mn/Zn site mutant (C-F), or the [delta]Zn site mutant (C-F), and in the (A, B, E, & F) presence or (A-D) absence of 0.1 mM PQ. Expression data are normalized to growth. Error bars indicate SEM (n = 3 or more). (A & B) # = p <0.05 via two-way ANOVA with Tukey's post-test for the indicated comparison. * = p <0.05 via two-way ANOVA with Tukey's post-test relative to bacteria grown in the presence of paraquat without CP. ^ = p <0.05 via two-way ANOVA with Tukey's post-test relative to bacteria grown in the absence of both paraquat and CP (C-F) * = p <0.05 via two-way ANOVA with Dunnett's post-test when compared to wild type CP.

http://dx.doi.org/10.1371/journal.ppat.1006125.g001

We also evaluated the impact of oxidative stress on the expression of the SODs using the superoxide-generating compound paraquat (PQ). Similar to previous studies [42], PQ induced the expression of sodA in metal-replete media (Fig 1A). However, PQ did not alter the induction of expression of sodM observed with CP (Fig 1B), nor did it change the sodA and sodM expression pattern observed with the [delta]Mn/Zn or [delta]Zn site mutants (Fig 1E & 1F). Cumulatively, these observations suggest that the expression of SodM increases when S. aureus is Mn-limited regardless of level of oxidative stress experienced by S. aureus.

The activity of the staphylococcal SODs does not correlate with gene expression

The propensity of Mn/Fe-SODs to acquire the wrong metal can result in discordance between expression levels and enzymatic activity [46]. In order to determine if SodA and SodM activity correlated with expression, total and individual SOD activity were assessed in the presence and absence of CP and PQ. Consistent with prior results, CP significantly reduced total staphylococcal SOD activity [14] (S1A Fig). In the absence of CP, the predominant activity comes from SodA (Fig 2A & 2B). The presence of the SodA/SodM heterodimer [41, 43] indicates that SodM is present in Mn-replete media, although the SodM homodimer's activity is barely detectable in the gel. In the presence of CP, the relative contribution of SodA to total SOD activity decreased while that of SodM increased (Fig 2A & 2B). Notably, not only did the fractional contribution of SodM change, the absolute level of SodM activity also increased (Figs 2A & S1C). Consistent with prior studies, the addition of PQ increased total staphylococcal SOD activity [14] (S1A Fig). However, the addition of PQ did not change the impact that Mn availability had on the relative contributions of SodA and SodM to total staphylococcal SOD activity (Figs 2B, S1B & S1C). Together, these results indicate that in Mn-replete environments SodA is the primary source of SOD activity but SodM becomes the predominant SOD when S. aureus experiences Mn starvation.

Download:

*

PPT

PowerPoint slide

*

PNG

larger image

*

TIFF

original image

Fig 2. SodM is the predominant source of SOD activity in Mn-deplete environments.

Wild type S. aureus was grown in various concentrations of CP in the (A & B) absence and (B) presence of 0.1 mM PQ, and both (A) the individual activities of the SODs and (B) the fractional contribution of SodA and SodM to total SOD activity in cell lysates (5.17 Mg of total protein) was determined. The lower gel was treated with hydrogen peroxide prior to assessing SOD activity to inactivate Fe-containing SODs. Purified recombinant SodA and SodM (0.3 Mg), loaded with either Mn or Fe, were included as controls. The experiment was repeated 3 times and representative gels are shown.

http://dx.doi.org/10.1371/journal.ppat.1006125.g002

SodA and SodM differentially contribute to resisting oxidative stress based on Mn availability

To test the respective contribution of each SOD to resisting Mn starvation, the ability of [delta]sodA and [delta]sodM single mutants, as well as a [delta]sodA[delta]sodM double mutant, to grow in the presence of CP was assessed. Similar to previous results [14, 42], [delta]sodA[delta]sodM was profoundly more sensitive to CP and PQ than wild type (Fig 3A & 3B), while ectopic expression of either SodA or SodM reversed this sensitivity (S2 Fig). In the absence of PQ, [delta]sodA grew as well as wild type S. aureus in both the presence and absence of CP (Fig 3A), whereas the [delta]sodM mutant, although it did not reach significance, displayed consistent reduced growth relative to wild type at high levels of CP (Fig 3A). Given the role of SodA and SodM in detoxifying superoxide, we also evaluated the impact of Mn availability on the ability of [delta]sodA and [delta]sodM to resist oxidative stress. Consistent with the activity analysis and its reported role as the primary SOD expressed by S. aureus [41, 42], loss of SodA resulted in increased sensitivity to PQ in the absence of CP (Fig 3B). However, at high concentrations of CP [delta]sodA was no more sensitive to PQ than wild type S. aureus (Fig 3B). When compared to [delta]sodA, the impact that CP had on the sensitivity of [delta]sodM to oxidative stress was reversed; the [delta]sodM mutant was no more sensitive to PQ than wild type bacteria in the absence and presence of low concentrations of CP, but the mutant was significantly more sensitive at high concentrations (Fig 3B). Notably, in the presence of intermediate CP concentrations in which both SodA and SodM are active, neither [delta]sodA nor [delta]sodM is more sensitive than WT S. aureus to oxidative stress. Utilization of the CP metal-binding site mutants revealed that both in the presence and absence of PQ, the increased sensitivity of [delta]sodM is due to Mn sequestration (Figs 3C, 3D, S2C & S2D). Cumulatively, these results indicate that in Mn-replete environments SodA is primarily responsible for protecting S. aureus from oxidative stress, whereas SodM is critical for protecting S. aureus from oxidative stress in Mn-deplete environments. They also suggest that SodM promotes resistance to nutritional immunity by facilitating the retention of SOD activity and resistance to oxidative stress.

Download:

*

PPT

PowerPoint slide

*

PNG

larger image

*

TIFF

original image

Fig 3. SodM is required to resist CP-imposed Mn starvation.

Wild type, [delta]sodA, [delta]sodM and [delta]sodA[delta]sodM S. aureus were grown in the presence of increasing concentrations of (A & B) WT CP, (C) the [delta]Mn/Zn site mutant, or (D) the [delta]Zn site mutant in the (A) absence and (C-D) presence of 0.1 mM PQ. Growth was assessed by measuring optical density (OD600). * = p<0.05 relative to wild type at the same concentration of CP via two-way ANOVA with Dunnett's post-test. Error bars indicate SEM (n = 3 or more).

http://dx.doi.org/10.1371/journal.ppat.1006125.g003

SodM is cambialistic

Paradoxically, our results indicate that the reportedly Mn-dependent enzyme SodM promotes resistance to host-imposed Mn starvation. In light of these observations, we analyzed the metal specificities of recombinant SodA and SodM. To facilitate these studies, the SODs were expressed in and purified from E. coli grown in iron-replete media. Following expression in E. coli and consistent with negligible Mn accumulation by E. coli in the absence of oxidative stress [46], inductively coupled plasma mass spectrometry (ICP-MS) analysis revealed that both of the purified recombinant staphylococcal SODs were loaded with Fe when recovered from the heterologous host (S3 Fig). Substantial activity was observed with purified Fe-SodM (210 +/- 21 U/mg protein), but negligible activity was detected from Fe-SodA (4 +/- 1 U/mg). Each of the recombinant proteins were denatured in the presence of metal chelators and then refolded in the presence of Mn in vitro, with successful elimination of Fe and loading with Mn confirmed by ICP-MS (S3 Fig). Enzymatic analysis revealed that the Mn-SodA form has substantial activity (1594 +/- 81 U/mg) in contrast to the Fe form. Surprisingly, Mn-SodM was also active (215 +/- 21 U/mg) and to a degree similar to that of the Fe-SodM, although both forms display activity substantially lower than that of Mn-SodA. The comparable activity of the Mn- and Fe-loaded forms of SodM indicate that it is not Mn-dependent, as previously suggested, but cambialistic [43].

Cambialism enables SodM to retain activity when S. aureus is Mn-starved

The cambialistic properties of SodM raise the possibility that in Mn-deficient conditions, including those induced by the presence of CP, Fe-loaded SodM predominates in the cell. We took advantage of the fact that Fe-dependent SODs can be selectively inactivated by hydrogen peroxide to evaluate if both the Mn- and Fe-loaded forms of SodM are present in S. aureus [31]. For these experiments, SodM was expressed from a plasmid in [delta]sodA[delta]sodM and SOD activity was assessed following growth in Fe- and Mn-replete media. Control experiments using purified protein confirmed that the Fe-loaded form of SodM, but not the Mn-loaded forms of SodA or SodM, is sensitive to peroxide poisoning (Fig 2A). Consistent with prior studies [43], following growth in Mn-replete media SodM activity was not affected by peroxide indicating that the protein is loaded with Mn. However, when grown in Fe-replete media SodM activity was sensitive to hydrogen peroxide, indicating that it was Fe-loaded (Fig 4). These results indicate that in S. aureus SodM can be active with either Mn or Fe. CP has been observed to bind Fe2+, although it is unclear if this binding contributes to antimicrobial activity [13, 47]. As such, it raises the possibility that when exposed to CP S. aureus may be incapable of populating SodM with Fe. To evaluate the metallation state of SodM, lysates from cells cultured in the presence of CP were treated with H2O2 and assayed for SOD activity. In the absence of CP there was no reduction in activity associated with the heterodimer following peroxide treatment indicating that SodM is loaded with Mn. However, in the presence of CP, peroxide treatment eliminated almost all SodM activity, indicating that it is loaded with Fe (Fig 2A). Oxidative stress did not change the metal that was associated with SodM in both the presence and absence of CP (S1B Fig). Cumulatively, these observations suggest that the cambialistic nature of SodM enables S. aureus to resist host-imposed Mn starvation by facilitating the retention of SOD activity.

Download:

*

PPT

PowerPoint slide

*

PNG

larger image

*

TIFF

original image

Fig 4. SodM can be metallated with either Mn or Fe in S. aureus.

The S. aureus [delta]sodA[delta]sodM mutant expressing SodM from a plasmid was grown in NRPMI supplemented with either 1 MM FeCl2 or 1 MM MnCl2 and SOD activity was assessed in cell lysates (24.8 Mg of total protein), with and without peroxide treatment. Peroxide treatment was used to inactivate Fe-containing SODs. Purified SodM (0.3 Mg), loaded with either Mn or Fe, were included as controls. The experiment was repeated 3 times, and representative gels are shown.

http://dx.doi.org/10.1371/journal.ppat.1006125.g004

SodM enhances the ability of S. aureus to resist Mn starvation during infection

In order to evaluate if SodA or SodM differentially contribute to pathogenesis based on Mn abundance during infection, we took advantage of the difference in Mn availability in wild type and CP-deficient mice [9]. Initially, the respective contributions of SodA and SodM to systemic disease in wild type C57BL/6 mice, in which the staphylococcal abscess is devoid of Mn, was assessed. In wild type mice, infection with [delta]sodA resulted in a modest, but not significant, reduction in bacterial burden when compared to wild type S. aureus. In contrast, in wild type mice infected with [delta]sodM there was a significant reduction in bacterial burden relative to wild type S. aureus (Fig 5), indicating that in the absence of Mn, SodM is critical for staphylococcal infection. Next, we infected CP-deficient mice, which fail to sequester Mn from staphylococcal liver abscesses [9]. Consistent with prior results, higher bacterial burdens were recovered from CP-deficient mice (C57BL/6 S100A9-/-) than wild type C57BL/6 mice infected with wild type S. aureus [9] (Fig 5). CP-deficient mice infected with [delta]sodA had significantly reduced bacterial burdens when compared to wild type S. aureus. This result contrasts with CP-deficient mice infected with [delta]sodM, which had bacterial burdens comparable to that of those infected with wild type S. aureus. In total, these observations indicate that SodA, but not SodM, contributes to staphylococcal disease when Mn is abundant. They also support the hypothesis that SodM contributes to the ability of S. aureus to resist host-imposed Mn starvation during infection.

Download:

*

PPT

PowerPoint slide

*

PNG

larger image

*

TIFF

original image

Fig 5. SodM contributes to resisting Mn starvation during infection.

Wild type C57BL/6 (C57) and C57BL/6 S100A9-/- (CP-/-) mice were infected with either wild type, [delta]sodA or [delta]sodM S. aureus. Mice were sacrificed 96 h after infection, and bacterial loads in the livers were enumerated. p <0.05 as determined by Mann-Whitney test.

http://dx.doi.org/10.1371/journal.ppat.1006125.g005

Discussion

During infection the innate immune system combats invading microbes by restricting the availability of the essential nutrient Mn [9, 12, 14]. At the same time, S. aureus and other pathogens must also overcome other host defenses including the oxidative burst of immune cells [23]. Accomplishing this latter task is made more challenging, as host-imposed Mn starvation inactivates bacterial Mn-dependent SODs [14]. The current investigations revealed that the possession of a cambialistic SOD enables S. aureus to counter these dual host threats both in culture and during infection. This strategy represents an entirely new mechanism for resisting host-imposed Mn starvation and establishes that cambialistic SODs contribute to bacterial pathogenesis.

The Fe/Mn superfamily of SODs is widely distributed in bacteria, archaea, and eukaryotes. Members of this family are generally thought to be reliant on either Mn or Fe for catalytic activity [28, 30, 48]. However, since the 1980s, predominantly in vitro analyses have suggested that a subset of these enzymes, termed cambialistic SODs, are capable of using both Fe and Mn, [32-34, 38-40]. Cambialistic SODs have been reported in both Gram-positive and Gram-negative bacteria, including the human pathogens Porphyromonas gingivalis, Streptococcus pneumoniae, and Streptococcus mutans and suggested to be present in other microbes including Bacteroides fragilis, and Bacteroides thetaiotaomicron [32-40, 49-52]. However, the lack of detailed in vivo studies and the fact that many cambialistic SODs have greater activity when loaded with one or the other cofactor in vitro has resulted in skepticism regarding the importance of cambialism [32, 36, 38-40]. As such, a false dichotomy exists that members of the Fe/Mn SOD family must use either Mn or Fe but not both. This dichotomy has led to confusion over the biologically relevant metal utilized by several bacterial SODs, especially given the difficulty in predicting the cofactor utilized by this family of enzymes using bioinformatics [28]. The observation that SodM has equal activity with either Mn or Fe in vitro, can be activated with both metals in vivo, and promotes resistance to nutritional immunity during infection establishes a clear and important role for cambialistic SODs in facilitating resistance to host defenses. Given the ubiquity of CP and host-imposed metal starvation during infection, it seems likely that expression of a cambialistic SOD would provide a benefit to other pathogens as well. Cambialistic SODs are also found in a diverse collection of environmental microbes [32, 33, 35, 39], suggesting cambialism may represent a generalized strategy used by organisms to maintain a defense against superoxide in niches where Fe and Mn availability can fluctuate.

While metal-dependent mononuclear enzymes have historically been thought to utilize a specific cofactor, it has become apparent that there can be significant plasticity in the metal cofactor they can utilize, particularly in the case of Mn- and Fe-utilizing enzymes [53]. In response to peroxide stress E. coli and many other pathogens sequester intracellular Fe and increase the expression of Mn importers, which in turn leads to accumulation of this metal [53-55]. In E. coli, this action results in Fe-utilizing enzymes, such as ribulose-5-phosphate 3-epimerase, becoming populated with Mn [53, 56, 57]. This change in cofactors enables E. coli to both maintain enzymatic activity and prevent Fenton chemistry-induced damage, which can arise from the interaction of Fe2+ with oxidants [53, 56, 57]. Notably, in many Fe-centric organisms, including E. coli, Salmonella typhimurium, and Yersinia pestis, Fe starvation increases the expression of Mn uptake systems and the accumulation of Mn [54, 58-60]. In addition to enabling bacteria to activate Mn-dependent isozymes [61], the increased Mn levels may also allow them to replace Fe with Mn in non-redox enzymes. These observations in conjunction with our findings suggest that populating metalloenzymes with an alternative yet catalytically active metal may be a general strategy used by bacteria to survive when a specific metal is limiting. While specific examples for metals other than Fe and Mn are currently lacking, conceptually this cofactor plasticity may enable microbes to maintain critical metabolic processes when limited for other essential metals.

Amongst the staphylococci, S. aureus is the most pathogenic species and the only one that expresses two SODs [41-44], with SodM presumably being gained through duplication and subsequent divergence. However, the advantage that S. aureus gains by expressing two Mn-dependent SODs, which are 75% identical at the level of their primary sequence, had not been apparent. Our current studies found that SodM is induced by CP-imposed Mn-starvation. The observation that SodM is cambialistic and enables S. aureus to maintain SOD activity when Mn starved by the host provides a rationale for its acquisition. It also suggests a model in which the Mn-dependent SodA is important during the initial colonization of a tissue, while SodM becomes important later during infection following the imposition of Mn starvation by the host immune response. Notably, S. aureus is not the only pathogen to express multiple superoxide dismutases that initially appear to be functionally redundant in culture, but upon subsequent analysis possesses properties that enhance fitness in the context of pathogenesis [62, 63]. For example, a second Cu/Zn SOD expressed by some Salmonella is protease-resistant and binds to peptidoglycan, which enables it to retain activity and promote survival within the phagolysosome [63]. Cumulatively, these observations and our results emphasize the importance of evaluating the contribution of apparently redundant SODs to resisting oxidative stress in the context of the other stressors that an organism encounters within its ecological niche.

The antimicrobial activity of CP is generally thought to be mediated by the sequestration of Mn2+ and Zn2+ [9, 20, 21]. However, CP was recently shown to bind Fe2+, resulting in the suggestion that Fe restriction is a primary driver of its antimicrobial activity [47]. Notably, CP does not bind Fe3+, the ionic state that exists in oxidizing environments such as sites of infection [9, 20, 47]. Additionally, several experimental lines of evidence suggest that Mn limitation contributes to the antimicrobial activity of CP both in culture and during infection. In both Acinetobacter baumannii and S. aureus, CP reduces intracellular Mn but not Fe levels [17, 64]. The current observation that S. aureus replaces Mn in SodM with Fe even in the presence of concentrations of CP approaching 1 mg/ml further supports the idea that in culture CP is not imposing Fe limitation on S. aureus. Furthermore, in wild type mice loss of MntABC and MntH, the two Mn importers expressed by S. aureus, results in a substantial reduction in virulence; however, this defect is completely reversed in CP-deficient mice [13]. The observation that SodM is critical for infection in wild type but not CP-deficient mice further supports the idea that Mn but not Fe sequestration by CP contributes to controlling infection. Perhaps not surprisingly, given the myriad of high affinity staphylococcal Fe acquisition systems [65], it also suggests that during infection S. aureus more successfully competes with the host for Fe than Mn. Cumulatively, these findings strongly support, at least in the case of S. aureus and A. baumannii, that Mn and not Fe sequestration significantly contributes to the antimicrobial activity of CP.

Antibiotic resistance is a serious and growing threat to human health, with multiple agencies calling for the development of new approaches to treat bacterial infections [1, 2]. Understanding how pathogens overcome innate immune defenses has the potential to reveal new opportunities for therapeutic intervention. Our studies reveal a new mechanism by which bacteria can overcome a two-pronged attack by the host. They also clearly demonstrate a role for cambialism in resisting nutritional immunity and bacterial pathogenesis. Moreover, these results provide newfound importance for a neglected family of proteins that is widely distributed throughout the tree of life.

Methods

Ethics Statement

All animal work was approved by the Vanderbilt University Institutional Animal Care and Use Committee (protocol #M1600123) and was performed in accordance with United States Public Health Service Policy on Humane Care and Use of Laboratory Animals and the US Animal Welfare Act.

Bacterial Strains

Staphylococcus aureus strain Newman was used unless otherwise indicated. All strains and plasmids used in this study are listed in Tables 1 and 2. S. aureus was routinely grown in tryptic soy broth (TSB) and on tryptic soy agar plates (TSA), while E. coli was routinely cultivated in Luria Broth (LB) and on Luria agar plates. Both species were grown at 37°C. As needed for plasmid maintenance or gene inductions, 10 Mg/ml of chloramphenicol, 50 Mg/ml of kanamycin, 100 Mg/ml of ampicillin, or 10 ng/ml of anhydrotetracycline was included in the media used. All strains were stored at -80°C in media containing 30% glycerol.

Download:

*

PPT

PowerPoint slide

*

PNG

larger image

*

TIFF

original image

Table 1. Strains used in this study.

http://dx.doi.org/10.1371/journal.ppat.1006125.t001

Download:

*

PPT

PowerPoint slide

*

PNG

larger image

*

TIFF

original image

Table 2. Plasmids used in this study.

http://dx.doi.org/10.1371/journal.ppat.1006125.t002

Construction of staphylococcal mutants and plasmids

The [delta]sodA and [delta]sodM mutants were created via Phi85 transduction of the sodA::tet and sodM::erm alleles from RN6390 [43]. The staphylococcal SodA and SodM expression constructs were created by amplifying sodA and sodM using the primers indicated in Table 3 and then cloning these fragments into the anhydrotetracycline-inducible plasmid pRMC2 [66]. To generate the YFP reporter plasmids the promoters for sodA and sodM were amplified with the primers listed in Table 3 and then cloned using standard techniques into pAH5 [67]. To generate the promoterless YFP construct pAH5 was digested with PstI and KpnI to remove the existing promoter, blunted and then self-ligated.

Download:

*

PPT

PowerPoint slide

*

PNG

larger image

*

TIFF

original image

Table 3. Primers used in this study.

Underlined bases represent restriction sites used for subcloning, and bases highlighted in bold represent mutagenized bases.

http://dx.doi.org/10.1371/journal.ppat.1006125.t003

CP growth assays

Calprotectin growth assays were performed largely as previously described [13, 20], with the exception that overnight cultures were performed in Chelex-treated RPMI + 1% casamino acids (NRPMI) supplemented with 1 mM MgCl2, 100 MM CaCl2, and 1 MM FeCl2. These cultures were diluted 1:100 into 100 Ml of culture medium in a 96-well round-bottom plate and incubated at 37°C and with shaking at 180 rpm. The culture medium consisted of 38% TSB and 62% CP buffer (3 mM CaCl2, 20mM Tris base, and 100 mM NaCl, 10 mM [Beta]-mercaptoethanol, pH 7.5) supplemented with 1 MM MnCl2 and 1 MM ZnSO4. Where indicated 0.1 mM PQ was added to the media. The same growth conditions were utilized for the expression studies. For both growth and expression assays, optical density (OD600) and fluorescence was assessed after 8 hrs of growth.

SOD activity

Total and individual superoxide dismutase (SOD) activity were assayed using a water-soluble tetrazolium salt assay and a gel-based nitro blue tetrazolium assay, respectively, as previously described [14, 68]. For both assays, the bacteria were grown as for the CP assays and harvested in exponential phase (OD600 of ~ 0.3-0.35). The cells were collected and then resuspended in 0.5 mM KPO4 buffer at pH 7.8 with 0.1 mM EDTA [46]. The bacteria were then lysed via mechanical disruption and centrifuged to remove insoluble material. The protein concentration in the cell lysate was determined via BCA assay (Pierce). Total SOD activity was assessed using the SOD Assay Kit (Sigma-Aldrich), per the manufacturer's instructions. To evaluate the individual activity of each SOD, the cell lysates were normalized to total protein concentration and resolved on 10% native polyacrylamide gel. The gels were incubated in buffer containing 0.05 M potassium phosphate pH 7.8, 1 mM EDTA, 0.25 mM nitro blue tetrazolium chloride, and 0.05 mM riboflavin and then exposed to light, as previously described [68]. To evaluate if SOD activity was due to iron-loading, prior to assessing activity the gels were incubated with 20 mM H2O2 or water for 20 minutes. Gels were imaged using a BioRad imager Universal Hood II and the fractional distribution of SOD activity was determined using the BioRad Quantity One software.

Cloning of the sodA and sodM genes for heterologous expression

The sodA and sodM genes were amplified by PCR from S. aureus genomic DNA using Pfu polymerase (NEB) and the primer pairs sodA_for and sodA_rev and sodM_for and sodM_rev, respectively, which incorporated 5' NdeI and 3' BamHI restriction sites. PCR products were A-tailed with Taq polymerase (NEB) and cloned into the pGEM-T vector (Promega) to yield pGEM-T-sodA and pGEM-T-sodM, respectively. An internal NdeI site in the sodM sequence was silently mutated by site-directed mutagenesis using the primer pair sodMqc1_for and sodMqc1_rev to yield pGEM-T-sodMqc. The genes were sub-cloned through NdeI/BamHI (NEB) digestion of the pGEM-T constructs, purification of the gene inserts by agarose gel electrophoresis, and subsequent ligation into NdeI/BamHI-digested pET29a vector (Novagen) to yield pET29a-sodA and pET29a-sodM constructs. Both pET29a constructs were sequenced (GATC Biotech, Germany). A sequence error detected in pET29a-sodM (deletion of base A570) was subsequently corrected through site-directed mutagenesis using primers sodMqc2_for and sodMqc2_rev, and the final construct confirmed through sequencing.

Expression, purification and quantification of recombinant SodA and SodM

The pET29a-sodA and pET29a-sodM constructs were transformed into Escherichia coli BL21 ([Lambda]DE3) cells and selected on LB agar plates containing 50 Mg/ml kanamycin. For each cell type, cells were inoculated into M9 medium containing 10 MM FeSO4 and 50 Mg/ml kanamycin and cultured overnight at 37°C with 180 rpm orbital shaking. This overnight culture was used to inoculate 1 L M9 medium containing 10 MM FeSO4 and 50 Mg/ml kanamycin, cultured at 37°C with 180 rpm orbital shaking. At OD600 ~0.5, protein expression was induced by addition of 1 mM isopropyl [Beta]-D-1-thiogalactopyranoside (IPTG) plus a further 20 MM FeSO4 and incubation for 4 h under the same conditions. Cells were harvested by centrifugation (20 min, 4,000 g, 4°C), washed in 20 mM Tris(hydroxymethyl)aminomethane (Tris), pH 7.5, 10 mM ethylenediaminetetraacetic acid (EDTA), followed by a further wash in 20 mM Tris, pH 7.5, 150 mM NaCl and stored at -20°C.

Cells were resuspended in 20 mM Tris, pH 7.5 and lysed by sonication (6 x 10 s, with 1 min intervals, on ice) and the lysate clarified by centrifugation (20 min, 19,000 g, 4°C). The soluble lysate was loaded onto a 5 ml HiTrap Q HP anion exchange chromatography (AEC) column (GE Healthcare), the column was washed with 5 column volumes (CV) of buffer (20 mM Tris, pH 7.5), followed by elution with a 9 CV linear NaCl gradient (0-1 M NaCl) in the same buffer, collecting 2 ml fractions, using an Äkta fast performance liquid chromatography (FPLC) system (GE Healthcare). Fractions were analyzed for protein by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). SodA eluted from AEC at ~230 mM NaCl, whereas SodM eluted at ~248 mM NaCl. Aliquots (1 ml) of the peak AEC fractions containing the recombinant protein were further purified using the Äkta FPLC by size exclusion chromatography (SEC) on a Superdex 200 16/60 column (GE Healthcare), resolved in 20 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7.5 at 1 ml/min, collecting 2 ml fractions.

Concentrations of purified recombinant SodA and SodM were determined from A280nm measurements, using the empirically determined extinction coefficients ([epsilon]280nm) of 62,681 M-1 cm-1 for SodA and 64,949 M-1 cm-1 for SodM, each derived from quantitative amino acid analysis (Alta Bioscience, UK). The metal content of the purified proteins was assessed by ICP-MS, as described below.

Unfolding and refolding of recombinant SOD proteins

To reconstitute recombinant SodA and SodM, which contained primarily Fe when purified from E. coli, with exclusively Mn they were unfolded and refolded in excess Mn, as previously described [69], with modifications. Unfolding was performed in 2.5 M guanidine hydrochloride, in the presence of 5 mM EDTA and 20 mM 8-hydroxyquinoline to remove bound metal ions, followed by refolding through several rounds of dialysis against 20 mM Tris, 100 mM NaCl, 10 mM MnCl2, pH 7.5, to yield protein containing exclusively manganese. To analyze the bound metal, aliquots of each purified protein (~2 mg in 0.5 ml) were resolved on a Superdex 200 Increase 10/30 column (GE Healthcare) in 20 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7.5, resolved at 0.75 ml/min and collecting 0.5 ml fractions using the Äkta FPLC. Eluant fractions were analyzed for protein by A280nm and by SDS-PAGE, for elemental composition by ICP-MS, and for enzyme activity by in-gel activity assay.

Elemental analysis by inductively coupled plasma mass spectrometry

For elemental analysis via ICP-MS, protein-containing samples were diluted 50-fold into a solution of 2.5% HNO3 (Suprapur, Merck) containing 20 Mg/l Co and Pt as internal standards. Matrix-matched elemental standards (containing analyte metal concentrations of 0-500 Mg/L) were prepared by serial dilution from individual metal standard stocks (VWR) with identical solution compositions, including the internal standard. All standards and samples were analyzed by ICP-MS using a Thermo x-series instrument operating in collision cell mode (using 3.0 ml/min flow of 8% H2 in He as the collision gas). Isotopes 55Mn, 56Fe, 59Co, 66Zn, and 195Pt were monitored using the peak-jump method (100 sweeps, 20-30 ms dwell time on 3-5 channels per isotope, separated by 0.02 atomic mass units) in triplicate, and metal concentrations determined from the standard curve.

Animal infections

Nine-week-old female black C57BL/6 and CP -/- (C57BL/6 S100A9-/-) mice were infected using a retro-orbital infection model [9, 13, 14]. Livers were harvested and homogenized 96 hours post-infection. Serial dilutions were then plated and counted for colony forming units.

Supporting Information

S1 Fig. SodM is the predominant source of SOD activity in Mn-deplete environments regardless of oxidative stress.

Wild type S. aureus and [delta]sodA[delta]sodM were grown in the presence of CP, in the (A-C) presence and (A & B) absence of 0.1 mM PQ and (A) total SOD activity and (C) the individual contributions of SodA and SodM to SOD activity were determined (n = 3). (B) In-gel analysis of the individual activities of SodA and SodM following growth in the presence of CP and PQ. Hydrogen peroxide treatment was used to inactivate Fe-containing SODs. The experiment was repeated 3 times and representative gels are shown. * = p <0.05 relative to no CP via two-way ANOVA with Tukey's post-test.

(TIF)

S2 Fig. Contribution of SodA and SodM to resisting oxidative stress and metal limitation.

(A & B) The [delta]sodA[delta]sodM mutant expressing either SodA or SodM from a plasmid was grown in various concentrations of CP in the (A) absence and (B) presence of 0.1 mM PQ. Growth (OD600) was measured after 8 h. * = p <0.05 relative to WT containing empty vector via two-way ANOVA with Dunnett's post-test. Error bars indicate SEM (n = 3 or more). (C-D) Wild type S. aureus, [delta]sodA, [delta]sodM, and [delta]sodA[delta]sodM were grown in the presence of various concentrations of (C) the [delta]Mn/Zn site CP mutant or (D) the [delta]Zn site mutant in the absence of PQ. * = p <0.05 relative to wild type at the same concentration of CP via two-way ANOVA with Dunnett's post-test. Error bars indicate SEM (n = 3 or more).

(TIF)

S3 Fig. Elemental analysis of purified and remetallated SodA and SodM.

Aliquots (~2 mg in 0.5 ml) of purified recombinant (A) SodA and (B) SodM were resolved by analytical size exclusion chromatography and eluant fractions (0.5 ml) were analyzed for protein by A280nm (black), and for manganese (blue) and iron (red) content by ICP-MS. Both proteins contained exclusively iron when purified from the heterologous host. Each protein was then unfolded, stripped of iron, and refolded in the presence of manganese. The resulting proteins were analyzed identically, and the refolded (C) SodA and (D) SodM were found to contain exclusively manganese. (E) Each of the four resulting samples (Fe-SodA, Fe-SodM, Mn-SodA and Mn-SodM) were subjected to protein analysis by SDS-PAGE, and to both in-gel and spectrophotometric SOD activity analysis.

(TIF)

Acknowledgments

We would like to thank the members of the Kehl-Fie and Waldron research groups for their critical reading of the manuscript.

Author Contributions

1. Conceptualization: TEKF YMG KJW ABS ET EPS.

2. Funding acquisition: TEKF KJW EPS.

3. Investigation: TEKF YMG KJW ABS ET.

4. Supervision: TEKF KJW EPS.

5. Visualization: TEKF YMG KJW ET ABS.

6. Writing - original draft: TEKF YMG KJW ABS ET.

7. Writing - review & editing: TEKF EPS KJW ET ABS YMG.

References

CDC. Antibiotic Resistance Threats in the United States. U.S. Centers for Disease Control and Prevention; 2013

Organization WH. Antimicrobial resistance: global report on surveillance. 2014

Lowy FD. Staphylococcus aureus infections. The New England journal of medicine. 1998;339(8):520-32. Epub 1998/08/26. doi: 10.1056/NEJM199808203390806. pmid:9709046

Klevens RM, Morrison MA, Nadle J, Petit S, Gershman K, Ray S, et al. Invasive methicillin-resistant Staphylococcus aureus infections in the United States. Jama. 2007;298(15):1763-71. Epub 2007/10/18. doi: 10.1001/jama.298.15.1763. pmid:17940231

Said-Salim B, Mathema B, Kreiswirth BN. Community-acquired methicillin-resistant Staphylococcus aureus: an emerging pathogen. Infection control and hospital epidemiology: the official journal of the Society of Hospital Epidemiologists of America. 2003;24(6):451-5. Epub 2003/06/28

Andreini C, Bertini I, Cavallaro G, Holliday GL, Thornton JM. Metal ions in biological catalysis: from enzyme databases to general principles. Journal of biological inorganic chemistry: JBIC: a publication of the Society of Biological Inorganic Chemistry. 2008;13(8):1205-18. Epub 2008/07/08

Waldron KJ, Rutherford JC, Ford D, Robinson NJ. Metalloproteins and metal sensing. Nature. 2009;460(7257):823-30. Epub 2009/08/14. doi: 10.1038/nature08300. pmid:19675642

Waldron KJ, Robinson NJ. How do bacterial cells ensure that metalloproteins get the correct metal? Nature reviews Microbiology. 2009;7(1):25-35. Epub 2008/12/17. doi: 10.1038/nrmicro2057. pmid:19079350

Corbin BD, Seeley EH, Raab A, Feldmann J, Miller MR, Torres VJ, et al. Metal chelation and inhibition of bacterial growth in tissue abscesses. Science. 2008;319(5865):962-5. Epub 2008/02/16. doi: 10.1126/science.1152449. pmid:18276893

Schaible UE, Kaufmann SH. Iron and microbial infection. Nature reviews Microbiology. 2004;2(12):946-53. Epub 2004/11/20. doi: 10.1038/nrmicro1046. pmid:15550940

Weinberg ED. Iron availability and infection. Biochimica et biophysica acta. 2009;1790(7):600-5. Epub 2008/08/05. doi: 10.1016/j.bbagen.2008.07.002. pmid:18675317

Kehl-Fie TE, Skaar EP. Nutritional immunity beyond iron: a role for manganese and zinc. Current opinion in chemical biology. 2010;14(2):218-24. Epub 2009/12/18 PubMed Central PMCID: PMC2847644. doi: 10.1016/j.cbpa.2009.11.008. pmid:20015678

Kehl-Fie TE, Zhang Y, Moore JL, Farrand AJ, Hood MI, Rathi S, et al. MntABC and MntH contribute to systemic Staphylococcus aureus infection by competing with calprotectin for nutrient manganese. Infection and immunity. 2013;81(9):3395-405. Epub 2013/07/03. PubMed Central PMCID: PMC3754211. doi: 10.1128/IAI.00420-13. pmid:23817615

Kehl-Fie TE, Chitayat S, Hood MI, Damo S, Restrepo N, Garcia C, et al. Nutrient metal sequestration by calprotectin inhibits bacterial superoxide defense, enhancing neutrophil killing of Staphylococcus aureus. Cell host & microbe. 2011;10(2):158-64. Epub 2011/08/17. PubMed Central PMCID: PMCPmc3157011

Gebhardt C, Nemeth J, Angel P, Hess J. S100A8 and S100A9 in inflammation and cancer. Biochemical pharmacology. 2006;72(11):1622-31. doi: 10.1016/j.bcp.2006.05.017. pmid:16846592

Clohessy PA, Golden BE. Calprotectin-mediated zinc chelation as a biostatic mechanism in host defence. Scandanavian Journal of Immunology. 1995;42(5):551-6

Hood MI, Mortensen BL, Moore JL, Zhang Y, Kehl-Fie TE, Sugitani N, et al. Identification of an Acinetobacter baumannii zinc acquisition system that facilitates resistance to calprotectin-mediated zinc sequestration. PLoS pathogens. 2012;8(12):e1003068. Epub 2012/12/14. PubMed Central PMCID: PMC3516566. doi: 10.1371/journal.ppat.1003068. pmid:23236280

Bianchi M, Niemiec MJ, Siler U, Urban CF, Reichenbach J. Restoration of anti-Aspergillus defense by neutrophil extracellular traps in human chronic granulomatous disease after gene therapy is calprotectin-dependent. The Journal of allergy and clinical immunology. 2011;127(5):1243-52 e7. doi: 10.1016/j.jaci.2011.01.021. pmid:21376380

Urban CF, Ermert D, Schmid M, Abu-Abed U, Goosmann C, Nacken W, et al. Neutrophil extracellular traps contain calprotectin, a cytosolic protein complex involved in host defense against Candida albicans. PLoS pathogens. 2009;5(10):e1000639. PubMed Central PMCID: PMCPMC2763347. doi: 10.1371/journal.ppat.1000639. pmid:19876394

Damo SM, Kehl-Fie TE, Sugitani N, Holt ME, Rathi S, Murphy WJ, et al. Molecular basis for manganese sequestration by calprotectin and roles in the innate immune response to invading bacterial pathogens. Proceedings of the National Academy of Sciences of the United States of America. 2013;110(10):3841-6. Epub 2013/02/23 PubMed Central PMCID: PMC3593839. doi: 10.1073/pnas.1220341110. pmid:23431180

Hayden JA, Brophy MB, Cunden LS, Nolan EM. High-affinity manganese coordination by human calprotectin is calcium-dependent and requires the histidine-rich site formed at the dimer interface. Journal of the American Chemical Society. 2013;135(2):775-87. PubMed Central PMCID: PMCPMC3575579. doi: 10.1021/ja3096416. pmid:23276281

Brophy MB, Hayden JA, Nolan EM. Calcium ion gradients modulate the zinc affinity and antibacterial activity of human calprotectin. Journal of the American Chemical Society. 2012;134(43):18089-100. Epub 2012/10/23. PubMed Central PMCID: PMC3579771. doi: 10.1021/ja307974e. pmid:23082970

Rigby KM, DeLeo FR. Neutrophils in innate host defense against Staphylococcus aureus infections. Seminars in immunopathology. 2012;34(2):237-59. PubMed Central PMCID: PMCPMC3271231. doi: 10.1007/s00281-011-0295-3. pmid:22080185

Beyer W, Imlay J, Fridovich I. Superoxide dismutases. Progress in nucleic acid research and molecular biology. 1991;40:221-53. Epub 1991/01/01. pmid:1851570

Imlay JA. Pathways of oxidative damage. Annual review of microbiology. 2003;57:395-418. Epub 2003/10/07. doi: 10.1146/annurev.micro.57.030502.090938. pmid:14527285

Lynch M, Kuramitsu H. Expression and role of superoxide dismutases (SOD) in pathogenic bacteria. Microbes and infection / Institut Pasteur. 2000;2(10):1245-55. Epub 2000/09/29

Storz G, Imlay JA. Oxidative stress. Current opinion in microbiology. 1999;2(2):188-94. Epub 1999/05/14. pmid:10322176

Sheng Y, Abreu IA, Cabelli DE, Maroney MJ, Miller AF, Teixeira M, et al. Superoxide dismutases and superoxide reductases. Chemical reviews. 2014;114(7):3854-918. PubMed Central PMCID: PMCPMC4317059. doi: 10.1021/cr4005296. pmid:24684599

Miller AF. Superoxide dismutases: ancient enzymes and new insights. FEBS letters. 2012;586(5):585-95. Epub 2011/11/15 doi: 10.1016/j.febslet.2011.10.048. pmid:22079668

Clare DA, Blum J, Fridovich I. A hybrid superoxide dismutase containing both functional iron and manganese. The Journal of biological chemistry. 1984;259(9):5932-6. pmid:6371011

Bray RC, Cockle SA, Fielden EM, Roberts PB, Rotilio G, Calabrese L. Reduction and inactivation of superoxide dismutase by hydrogen peroxide. The Biochemical journal. 1974;139(1):43-8. Epub 1974/04/01. PubMed Central PMCID: PMC1166249. pmid:4377099

Yamano S, Sako Y, Nomura N, Maruyama T. A cambialistic SOD in a strictly aerobic hyperthermophilic archaeon, Aeropyrum pernix. Journal of biochemistry. 1999;126(1):218-25. Epub 1999/07/07. pmid:10393342

Lancaster VL, LoBrutto R, Selvaraj FM, Blankenship RE. A cambialistic superoxide dismutase in the thermophilic photosynthetic bacterium Chloroflexus aurantiacus. Journal of bacteriology. 2004;186(11):3408-14. Epub 2004/05/20. PubMed Central PMCID: PMC415758. doi: 10.1128/JB.186.11.3408-3414.2004. pmid:15150226

Hiraoka BY, Yamakura F, Sugio S, Nakayama K. A change of the metal-specific activity of a cambialistic superoxide dismutase from Porphyromonas gingivalis by a double mutation of Gln-70 to Gly and Ala-142 to Gln. The Biochemical journal. 2000;345 Pt 2:345-50. Epub 2000/01/06. PubMed Central PMCID: PMC1220763. pmid:10620511

Mandelli F, Franco Cairo JP, Citadini AP, Buchli F, Alvarez TM, Oliveira RJ, et al. The characterization of a thermostable and cambialistic superoxide dismutase from Thermus filiformis. Letters in applied microbiology. 2013;57(1):40-6. Epub 2013/03/28. doi: 10.1111/lam.12071. pmid:23530753

Eijkelkamp BA, Morey JR, Ween MP, Ong CL, McEwan AG, Paton JC, et al. Extracellular zinc competitively inhibits manganese uptake and compromises oxidative stress management in Streptococcus pneumoniae. PloS one. 2014;9(2):e89427. Epub 2014/02/22. PubMed Central PMCID: PMC3928430. doi: 10.1371/journal.pone.0089427. pmid:24558498

De Vendittis A, Marco S, Di Maro A, Chambery A, Albino A, Masullo M, et al. Properties of a putative cambialistic superoxide dismutase from the aerotolerant bacterium Streptococcus thermophilus strain LMG 18311. Protein and peptide letters. 2012;19(3):333-44. Epub 2012/03/14. pmid:22409500

Meier B, Sehn AP, Michel C, Saran M. Reactions of hydrogen peroxide with superoxide dismutase from Propionibacterium shermanii--an enzyme which is equally active with iron or manganese--are independent of the prosthetic metal. Archives of biochemistry and biophysics. 1994;313(2):296-303. Epub 1994/09/01. doi: 10.1006/abbi.1994.1391. pmid:8080276

Tabares LC, Bittel C, Carrillo N, Bortolotti A, Cortez N. The single superoxide dismutase of Rhodobacter capsulatus is a cambialistic, manganese-containing enzyme. Journal of bacteriology. 2003;185(10):3223-7. Epub 2003/05/06. PubMed Central PMCID: PMC154076. doi: 10.1128/JB.185.10.3223-3227.2003. pmid:12730184

Martin ME, Byers BR, Olson MO, Salin ML, Arceneaux JE, Tolbert C. A Streptococcus mutans superoxide dismutase that is active with either manganese or iron as a cofactor. The Journal of biological chemistry. 1986;261(20):9361-7. Epub 1986/07/15. pmid:3722201

Clements MO, Watson SP, Foster SJ. Characterization of the major superoxide dismutase of Staphylococcus aureus and its role in starvation survival, stress resistance, and pathogenicity. Journal of bacteriology. 1999;181(13):3898-903. Epub 1999/06/29. PubMed Central PMCID: PMC93877. pmid:10383955

Karavolos MH, Horsburgh MJ, Ingham E, Foster SJ. Role and regulation of the superoxide dismutases of Staphylococcus aureus. Microbiology. 2003;149(Pt 10):2749-58. Epub 2003/10/03. doi: 10.1099/mic.0.26353-0. pmid:14523108

Valderas MW, Hart ME. Identification and characterization of a second superoxide dismutase gene (sodM) from Staphylococcus aureus. Journal of bacteriology. 2001;183(11):3399-407. Epub 2001/05/10. PubMed Central PMCID: PMC99638. doi: 10.1128/JB.183.11.3399-3407.2001. pmid:11344148

Valderas MW, Gatson JW, Wreyford N, Hart ME. The superoxide dismutase gene sodM is unique to Staphylococcus aureus: absence of sodM in coagulase-negative staphylococci. Journal of bacteriology. 2002;184(9):2465-72. Epub 2002/04/12. PubMed Central PMCID: PMC134988. doi: 10.1128/JB.184.9.2465-2472.2002. pmid:11948161

Ballal A, Manna AC. Regulation of superoxide dismutase (sod) genes by SarA in Staphylococcus aureus. Journal of bacteriology. 2009;191(10):3301-10. Epub 2009/03/17. PubMed Central PMCID: PMC2687179. doi: 10.1128/JB.01496-08. pmid:19286803

Anjem A, Varghese S, Imlay JA. Manganese import is a key element of the OxyR response to hydrogen peroxide in Escherichia coli. Molecular microbiology. 2009;72(4):844-58. Epub 2009/04/30 PubMed Central PMCID: PMC2776087. doi: 10.1111/j.1365-2958.2009.06699.x. pmid:19400769

Nakashige TG, Zhang B, Krebs C, Nolan EM. Human calprotectin is an iron-sequestering host-defense protein. Nature chemical biology. 2015;11(10):765-71. Epub 2015/08/25. PubMed Central PMCID: PMCPmc4575267. doi: 10.1038/nchembio.1891. pmid:26302479

Ose DE, Fridovich I. Manganese-containing superoxide dismutase from Escherichia coli: reversible resolution and metal replacements. Archives of biochemistry and biophysics. 1979;194(2):360-4. pmid:36037

Sugio S, Hiraoka BY, Yamakura F. Crystal structure of cambialistic superoxide dismutase from Porphyromonas gingivalis. European journal of biochemistry / FEBS. 2000;267(12):3487-95. Epub 2000/06/10

Gregory EM, Dapper CH. Isolation of iron-containing superoxide dismutase from Bacteroides fragilis: reconstitution as a Mn-containing enzyme. Archives of biochemistry and biophysics. 1983;220(1):293-300. pmid:6830240

Pennington CD, Gregory EM. Isolation and reconstitution of iron- and manganese-containing superoxide dismutases from Bacteroides thetaiotaomicron. Journal of bacteriology. 1986;166(2):528-32. PubMed Central PMCID: PMCPMC214636. pmid:3700336

Nakamura T, Torikai K, Uegaki K, Morita J, Machida K, Suzuki A, et al. Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1--insights into the enzyme mechanism and stability. The FEBS journal. 2011;278(4):598-609. Epub 2010/12/25. doi: 10.1111/j.1742-4658.2010.07977.x. pmid:21182595

Anjem A, Imlay JA. Mononuclear iron enzymes are primary targets of hydrogen peroxide stress. The Journal of biological chemistry. 2012;287(19):15544-56. PubMed Central PMCID: PMCPMC3346116. doi: 10.1074/jbc.M111.330365. pmid:22411989

Juttukonda LJ, Skaar EP. Manganese homeostasis and utilization in pathogenic bacteria. Molecular microbiology. 2015;97(2):216-28. PubMed Central PMCID: PMCPMC4631260. doi: 10.1111/mmi.13034. pmid:25898914

Kehres DG, Zaharik ML, Finlay BB, Maguire ME. The NRAMP proteins of Salmonella typhimurium and Escherichia coli are selective manganese transporters involved in the response to reactive oxygen. Molecular microbiology. 2000;36(5):1085-100. pmid:10844693

Sobota JM, Gu M, Imlay JA. Intracellular hydrogen peroxide and superoxide poison 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase, the first committed enzyme in the aromatic biosynthetic pathway of Escherichia coli. Journal of bacteriology. 2014;196(11):1980-91. PubMed Central PMCID: PMCPMC4010980. doi: 10.1128/JB.01573-14. pmid:24659765

Sobota JM, Imlay JA. Iron enzyme ribulose-5-phosphate 3-epimerase in Escherichia coli is rapidly damaged by hydrogen peroxide but can be protected by manganese. Proceedings of the National Academy of Sciences of the United States of America. 2011;108(13):5402-7. PubMed Central PMCID: PMCPMC3069151. doi: 10.1073/pnas.1100410108. pmid:21402925

Ikeda JS, Janakiraman A, Kehres DG, Maguire ME, Slauch JM. Transcriptional regulation of sitABCD of Salmonella enterica serovar Typhimurium by MntR and Fur. Journal of bacteriology. 2005;187(3):912-22. PubMed Central PMCID: PMCPMC545731. doi: 10.1128/JB.187.3.912-922.2005. pmid:15659669

Patzer SI, Hantke K. Dual repression by Fe(2+)-Fur and Mn(2+)-MntR of the mntH gene, encoding an NRAMP-like Mn(2+) transporter in Escherichia coli. Journal of bacteriology. 2001;183(16):4806-13. PubMed Central PMCID: PMCPMC99535. doi: 10.1128/JB.183.16.4806-4813.2001. pmid:11466284

Perry RD, Craig SK, Abney J, Bobrov AG, Kirillina O, Mier I Jr., et al. Manganese transporters Yfe and MntH are Fur-regulated and important for the virulence of Yersinia pestis. Microbiology. 2012;158(Pt 3):804-15. PubMed Central PMCID: PMCPMC3352115. doi: 10.1099/mic.0.053710-0. pmid:22222497

Martin JE, Imlay JA. The alternative aerobic ribonucleotide reductase of Escherichia coli, NrdEF, is a manganese-dependent enzyme that enables cell replication during periods of iron starvation. Molecular microbiology. 2011;80(2):319-34 PubMed Central PMCID: PMCPMC3097424. doi: 10.1111/j.1365-2958.2011.07593.x. pmid:21338418

Gleason JE, Galaleldeen A, Peterson RL, Taylor AB, Holloway SP, Waninger-Saroni J, et al. Candida albicans SOD5 represents the prototype of an unprecedented class of Cu-only superoxide dismutases required for pathogen defense. Proceedings of the National Academy of Sciences of the United States of America. 2014;111(16):5866-71. Epub 2014/04/09 PubMed Central PMCID: PMC4000858. doi: 10.1073/pnas.1400137111. pmid:24711423

Rushing MD, Slauch JM. Either periplasmic tethering or protease resistance is sufficient to allow a SodC to protect Salmonella enterica serovar Typhimurium from phagocytic superoxide. Molecular microbiology. 2011;82(4):952-63. PubMed Central PMCID: PMCPMC3220996. doi: 10.1111/j.1365-2958.2011.07884.x. pmid:22023457

Radin JN, Kelliher JL, Párraga Solórzano PK, Kehl-Fie TE. The two-component system ArlRS and alterations in metabolism enable Staphylococcus aureus to resist calprotectin-induced manganese starvation. PLoS pathogens. 2016;12(11):e1006040. doi: 10.1371/journal.ppat.1006040. pmid:27902777

Sheldon JR, Heinrichs DE. Recent developments in understanding the iron acquisition strategies of gram positive pathogens. FEMS microbiology reviews. 2015;39(4):592-630. doi: 10.1093/femsre/fuv009. pmid:25862688

Corrigan RM, Foster TJ. An improved tetracycline-inducible expression vector for Staphylococcus aureus. Plasmid. 2009;61(2):126-9. Epub 2008/11/11. doi: 10.1016/j.plasmid.2008.10.001. pmid:18996145

Malone CL, Boles BR, Lauderdale KJ, Thoendel M, Kavanaugh JS, Horswill AR. Fluorescent reporters for Staphylococcus aureus. Journal of microbiological methods. 2009;77(3):251-60. Epub 2009/03/07. PubMed Central PMCID: PMCPmc2693297. doi: 10.1016/j.mimet.2009.02.011. pmid:19264102

McCord JM. Analysis of superoxide dismutase activity. Current protocols in toxicology / editorial board, Mahin D Maines. 2001;Chapter 7:Unit7 3. Epub 2001/05/01

Kirby T, Blum J, Kahane I, Fridovich I. Distinguishing between Mn-containing and Fe-containing superoxide dismutases in crude extracts of cells. Archives of biochemistry and biophysics. 1980;201(2):551-5. pmid:6994652