Abstract

Background

Currently, there is no agreed standard for exploring the antimicrobial activity of wound antiseptics in a phase 2/ step 2 test protocol. In the present study, a standardised in-vitro test is proposed, which allows to test potential antiseptics in a more realistically simulation of conditions found in wounds as in a suspension test. Furthermore, factors potentially influencing test results such as type of materials used as test carrier or various compositions of organic soil challenge were investigated in detail.

Methods

This proposed phase 2/ step 2 test method was modified on basis of the EN 14561 by drying the microbial test suspension on a metal carrier for 1 h, overlaying the test wound antiseptic, washing-off, neutralization, and dispersion at serial dilutions at the end of the required exposure time yielded reproducible, consistent test results.

Results

The difference between the rapid onset of the antiseptic effect of PVP-I and the delayed onset especially of polihexanide was apparent. Among surface-active antimicrobial compounds, octenidine was more effective than chlorhexidine digluconate and polihexanide, with some differences depending on the test organisms. However, octenidine and PVP-I were approximately equivalent in efficiency and microbial spectrum, while polihexanide required longer exposure times or higher concentrations for a comparable antimicrobial efficacy.

Conclusion

Overall, this method allowed testing and comparing differ liquid and gel based antimicrobial compounds in a standardised setting.

Details

Title
Proposed phase 2/ step 2 in-vitro test on basis of EN 14561 for standardised testing of the wound antiseptics PVP-iodine, chlorhexidine digluconate, polihexanide and octenidine dihydrochloride
Author
Schedler, Kathrin; Assadian, Ojan; Brautferger, Uta; Muller, Gerald; Koburger, Torsten; Classen, Simon; Kramer, Axel
Publication year
2017
Publication date
2017
Publisher
BioMed Central
e-ISSN
14712334
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1873351679
Copyright
Copyright BioMed Central 2017