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Copyright Sveuciliste u Zagrebu, Prehramheno-Biotehnoloski Fakultet 2017

Abstract

In this study, the effects of temperature-shift (from 30 to 25 °C) and temperature-constant (at 30 °C) cultivation on the mass of Monascus fuliginosus CG-6 mycelia and concentration of the produced monacolin K (MK) were monitored. The expression levels of the MK biosynthetic genes of M. fuliginosus CG-6 at constant and variable culture temperatures were analysed by real-time quantitative polymerase chain reaction (RT-qPCR). The total protein was collected and determined by liquid chromatography-electrospray ionisation with tandem mass spectrometry (LC-ESI-MS/MS). Results showed that the maximum mycelial mass in temperature-shift cultivation was only 0.477 g of dry cell mass per dish, which was lower than that in temperature-constant cultivation (0.581 g of dry cell mass per dish); however, the maximum concentration of MK in temperature-shift cultivation (34.5 qg/mL) was 16 times higher than that in temperature-constant cultivation at 30 °C (2.11 qg/mL). Gene expression analysis showed that the expression of the MK biosynthetic gene cluster at culture temperature of 25 °C was higher than that at 30 °C, which was similar to the trend of the MK concentration, except for individual MK B and MK C genes. Analysis of differential protein expression revealed that 2016 proteins were detected by LC-ESI-MS/MS. The expression level of efflux pump protein coded by the MK I gene exhibited the same upregulated trend as the expression of MK I in temperature-shift cultivation. Temperature-shift cultivation enhanced the expression of proteins in the secondary metabolite production pathway, but suppressed the expression of proteins involved in the mycelial growth.

Details

Title
Effect of Temperatur e-Shift and Temperature-Constant Cultivation on the Monacolin K Biosynthetic Gene Cluster Expression in Monascus sp.
Author
Lin, Lin; Wang, Changlu; Li, Zhenjing; Wu, Huijia; Chen, Mianhua
Pages
40-47A
Section
original scientific paper
Publication year
2017
Publication date
2017
Publisher
Sveuciliste u Zagrebu, Prehramheno-Biotehnoloski Fakultet
ISSN
13309862
e-ISSN
13342606
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1890052769
Copyright
Copyright Sveuciliste u Zagrebu, Prehramheno-Biotehnoloski Fakultet 2017