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Received: September 16, 2016

Accepted: February 8, 2017

Summary

This article presents a novel formulation for preparation of Lactobacillus casei 01 encapsulated in soy protein isolate and alginate microparticles using spray drying method. A response surface methodology was used to optimise the formulation and the central composite face-centered design was applied to study the effects of critical material attributes and process parameters on viability of the probiotic after microencapsulation and in simulated gastrointestinal conditions. Spherical microparticles were produced in high yield (64 %), narrow size distribution (d50=9.7 µm, span=0.47) and favourable mucoadhesive properties, with viability of the probiotic of 11.67, 10.05, 9.47 and 9.20 log CFU/g after microencapsulation, 3 h in simulated gastric and intestinal conditions and four-month cold storage, respectively. Fourier-transform infrared spectroscopy confirmed the probiotic stability after microencapsulation, while differential scanning calorimetry and thermogravimetry pointed to high thermal stability of the soy protein isolate-alginate microparticles with encapsulated probiotic. These favourable properties of the probiotic microparticles make them suitable for incorporation into functional food or pharmaceutical products.

Key words: Lactobacillus casei 01, alginate, soy protein isolate, microparticles, spray drying, formulation optimisation

(ProQuest: ... denotes formulae omitted.)

Introduction

The modern lifestyle and consequences of unhealthy eating habits on the one hand, and the strong evidence of health benefits gained by probiotic consumption on the other have stimulated the food and pharmaceutical industry to produce novel probiotic ingredients and products, with estimated market share of approx. 36.7 billion dollars by 2018 (1). The probiotics have been found to be effective for a large number of gut disorders and for improving/maintaining the oral and urogenital health (2-4). Other studies have indicated protective cardiovascular effects and promising effects in cancer, brain, obesity, allergy and atopic diseases, and in combatting diseases where dysbiosis has been observed (5-9). However, providing and maintaining the required minimum of viable probiotic cells to exert health effects (107 CFU/g at a point of delivery) is a great challenge, considering their sensitivity to processing, storage and administration. Microencapsulation could be applied as an effective method for achieving these goals (10). Among many microencapsulation techniques, spray drying is one of the most challenging due to low costs, industrial application, high probiotic stability in the product and preserved cellular integrity during drying, when both process...

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