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About the Authors:
Xin Chen
Roles Formal analysis, Investigation, Validation, Visualization, Writing - original draft, Writing - review & editing
Current address: Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, United States of America
Affiliation: Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States of America
ORCID http://orcid.org/0000-0003-1871-4477
Kunal Poorey
Roles Data curation, Formal analysis
Current address: Department of Systems Biology, Sandia National Laboratories, Livermore, California, United States of America
Affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia, United States of America
ORCID http://orcid.org/0000-0003-3665-0235
Melissa N. Carver
Roles Investigation
Affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia, United States of America
Ulrika Müller
Roles Investigation, Validation
Affiliation: Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States of America
Stefan Bekiranov
Roles Formal analysis, Writing - review & editing
Affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia, United States of America
David T. Auble
Roles Conceptualization, Funding acquisition, Resources, Supervision, Writing - review & editing
* E-mail: [email protected] (DAB); [email protected] (DTA)
Affiliation: Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia, United States of America
David A. Brow
Roles Conceptualization, Funding acquisition, Resources, Supervision, Visualization, Writing - original draft, Writing - review & editing
* E-mail: [email protected] (DAB); [email protected] (DTA)
Affiliation: Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States of America
ORCID http://orcid.org/0000-0002-8028-014XAbstract
Transcriptome studies on eukaryotic cells have revealed an unexpected abundance and diversity of noncoding RNAs synthesized by RNA polymerase II (Pol II), some of which influence the expression of protein-coding genes. Yet, much less is known about biogenesis of Pol II non-coding RNA than mRNAs. In the budding yeast Saccharomyces cerevisiae, initiation of non-coding transcripts by Pol II appears to be similar to that of mRNAs, but a distinct pathway is utilized for termination of most non-coding RNAs: the Sen1-dependent or “NNS” pathway. Here, we examine the effect on the S. cerevisiae transcriptome of conditional mutations in the genes encoding six different essential proteins that influence Sen1-dependent termination: Sen1, Nrd1, Nab3, Ssu72, Rpb11, and Hrp1. We...