Abstract

The orchestration of intercellular communication is essential for multicellular organisms. One mechanism by which cells communicate is through long, actin-rich membranous protrusions called tunneling nanotubes (TNTs), which allow the intercellular transport of various cargoes, between the cytoplasm of distant cells in vitro and in vivo. With most studies failing to establish their structural identity and examine whether they are truly open-ended organelles, there is a need to study the anatomy of TNTs at the nanometer resolution. Here, we use correlative FIB-SEM, light- and cryo-electron microscopy approaches to elucidate the structural organization of neuronal TNTs. Our data indicate that they are composed of a bundle of open-ended individual tunneling nanotubes (iTNTs) that are held together by threads labeled with anti-N-Cadherin antibodies. iTNTs are filled with parallel actin bundles on which different membrane-bound compartments and mitochondria appear to transfer. These results provide evidence that neuronal TNTs have distinct structural features compared to other cell protrusions.

The architecture of functional TNTs is still under debate. Here, the authors combine correlative FIB-SEM, light- and cryo-electron microscopy approaches to elucidate the structure of TNTs in neuronal cells, showing that they form structures that are distinct form other membrane protrusions.

Details

Title
Correlative cryo-electron microscopy reveals the structure of TNTs in neuronal cells
Author
Sartori-Rupp, Anna 1 ; Cordero Cervantes Diégo 2 ; Pepe, Anna 2 ; Gousset Karine 3   VIAFID ORCID Logo  ; Delage Elise 2 ; Simon, Corroyer-Dulmont 1 ; Schmitt, Christine 1 ; Krijnse-Locker Jacomina 1 ; Zurzolo Chiara 2 

 Institut Pasteur, Unit of Technology and Service Ultra-structural Bio-Imaging, Paris, France (GRID:grid.428999.7) (ISNI:0000 0001 2353 6535) 
 Institut Pasteur, Membrane Traffic and Pathogenesis, Paris, France (GRID:grid.428999.7) (ISNI:0000 0001 2353 6535) 
 Institut Pasteur, Membrane Traffic and Pathogenesis, Paris, France (GRID:grid.428999.7) (ISNI:0000 0001 2353 6535) ; California State University, Fresno, Department of Biology, College of Science and Math, Fresno, USA (GRID:grid.253558.c) (ISNI:0000 0001 2309 3092) 
Publication year
2019
Publication date
Jan 2019
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1927803260
Copyright
This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.