About the Authors:
William G. Miller
*To whom correspondence should be addressed. E-mail: [email protected]
Affiliation: Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, United States of America
Craig T. Parker
Affiliation: Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, United States of America
Marc Rubenfield
Affiliation: Agencourt Bioscience Corporation, Beverly, Massachusetts, United States of America
George L. Mendz
Affiliation: School of Medicine, Sydney, The University of Notre Dame Australia, Broadway, New South Wales, Australia
Marc M. S. M. Wösten
Affiliation: Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
David W. Ussery
Affiliation: Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark
John F. Stolz
Affiliation: Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania, United States of America
Tim T. Binnewies
Affiliation: Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark
Peter F. Hallin
Affiliation: Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark
Guilin Wang
Affiliation: Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, United States of America
Joel A. Malek
Affiliation: Agencourt Bioscience Corporation, Beverly, Massachusetts, United States of America
Andrea Rogosin
Affiliation: Agencourt Bioscience Corporation, Beverly, Massachusetts, United States of America
Larry H. Stanker
Affiliation: Foodborne Contaminants Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, United States of America
Robert E. Mandrell
Affiliation: Produce Safety and Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Albany, California, United States of America
Introduction
The epsilon subdivision of the Gram-negative Proteobacteria comprises multiple genera contained within three major families: Campylobacteraceae, Helicobacteraceae and Nautiliaceae. The majority of well-characterized species in this subdivision are members of genera within the first two families, including Campylobacter, Arcobacter and Sulfurospirillum in the Campylobacteraceae, and Helicobacter and Wolinella in the Helicobacteraceae. Many of these species are pathogenic, e.g. Campylobacter jejuni [1] and Helicobacter pylori [2], and/or are associated with a particular host or hosts, e.g. Campylobacter upsaliensis [3] and Helicobacter mustelae [4]; however, several species are free-living, e.g. Sulfurospirillum spp. [5], and they are not considered to be pathogenic.
The genus Arcobacter is an unusual taxon within the epsilon subdivision in that it contains both pathogenic and free-living species found in a wide range of environments. Currently, Arcobacter contains four recognized species: A. butzleri [6], A. cryaerophilus [7], A. skirrowii [8] and A. nitrofigilis [9]. A. butzleri, A. cryaerophilus and A. skirrowii have been isolated from animals and humans [10], while A. nitrofigilis is a nitrogen-fixing bacterium isolated originally from Spartina aterniflora roots in an estuarine marsh [9]. In addition to these established Arcobacter species, three new species have been described recently: 1) the obligate halophile A. halophilus sp. nov., isolated from a Hawaiian hypersaline lagoon [11], 2) A. cibarius sp. nov., isolated from broiler carcasses [12] and 3) Candidatus A. sulfidicus, a sulfide-oxidizing marine organism that produces filamentous sulfur [13]. In addition, several potential, novel Arcobacter species, based so far on only 16S rDNA sequence data, have been identified in: the flora of deep-sea hydrothermal vents [14], hydrocarbon-contaminated seawater [15], a low-salinity petroleum reservoir [16], infected or dead coral surfaces [17], deep-sea sediments [18], tube worms [19], anaerobic sludge [20], and a circulated dairy wastewater lagoon [21]. These studies demonstrate clearly that the genus is associated strongly with fresh-water and marine environments. In fact, although A. butzleri, A. cryaerophilus and A. skirrowii have been isolated often from animals or food sources, they have been isolated frequently also from water or water systems [22]–[30].
Arcobacter butzleri is the best characterized of all Arcobacters. A. butzleri cells are small, spiral and motile [10], similar morphologically to the taxonomically–related Campylobacter. Nonetheless, notable differences exist between A. butzleri and Campylobacter spp. Classified initially as an “aerotolerant Campylobacter”, along with A. cryaerophilus [10], A. butzleri is able to grow aerobically, at variance with most Campylobacters which are microaerophilic. However, A. butzleri grows also under microaerobic and anaerobic conditions [10]; thus, this bacterium can grow at all oxygen concentrations. Additionally, Campylobacter spp. grow generally between 37°C and 42°C [31], whereas A. butzleri is more psychrophilic with a temperature range between 15°C and 37°C, although some strains can grow at 42°C [10]. Furthermore, A. butzleri is more halotolerant than most Campylobacter spp., with some strains able to grow at 3.5% NaCl [10].
While A. butzleri is isolated often from aqueous environments, it is isolated also from multiple animals and food sources. It has been found in pigs [32] and ground pork [33], [34], chicken carcasses [35], [36] and other poultry [37], as well as in beef [38], [39], lamb [34] and the feces of other animals [40]. A. butzleri has also been isolated increasingly from human diarrheal stool samples [41]–[47]. The clinical symptomatology described for A. butzleri typically includes diarrhea and recurrent abdominal cramps [10], although A. butzleri-related bacteremia has also been reported [48], [49]. Prouzet-Mauléon et al. [46] reported an isolation frequency of 1% from human clinical stool samples. Additionally, Vandenberg et al. [47] reported an isolation frequency of 3.5% from diarrheic stool samples. Although co-infection with other enteric pathogens was reported by Prouzet-Mauléon and Vandenberg, in the majority of samples (14/15 clinical stool samples and 55/67 patients, respectively) no other enteric pathogen was detected. Houf et al. reported that Arcobacters were isolated from 1.4% (7/500) of asymptomatic human stool samples [50]. However, all seven isolates were typed as A. cryaerophilus; A. butzleri was not isolated. Similarly, Vandenberg et al. [47] reported also that A. butzleri was isolated more frequently from diarrheic stool samples than from non-diarrheic stool samples. Thus, the isolation of A. butzleri from diarrheic stool samples is likely to be relevant clinically and is probably not due to the organism being merely a human commensal. Therefore, these data suggest strongly that A. butzleri is an emerging pathogen [10], where transmission, as with C. jejuni, occurs probably through consumption of contaminated food or water. The low level of incidence reported in human clinical samples is most likely an underestimate, due to sub-optimal isolation and/or detection methods [46].
Relatively little is known about A. butzleri, compared to other members of the epsilon subdivision, but the wealth of genomic information from other epsilonproteobacterial taxa provides a solid foundation to compare and contrast A. butzleri to its taxonomic relatives. The genomes of multiple species of Epsilonproteobacteria have been sequenced; these include: four strains of C. jejuni subsp. jejuni ([51]–[54]: strains NCTC 11168, RM1221, 81-176 and 81116, respectively); C. jejuni subsp. doylei strain 269.97 (CP000768.1); Campylobacter coli strain RM2228, Campylobacter lari strain RM2100 and C. upsaliensis strain RM3195 [52]; Campylobacter fetus subsp. fetus strain 82-40 (CP000487.1); Campylobacter curvus strain 525.92 (CP000767.1); Campylobacter concisus strain 13826 (CP000792.1); Campylobacter hominis strain ATCC BAA-381 (CP000776.1); Sulfuromonas denitrificans strain ATCC 33889 (formerly Thiomicrospira denitrificans [55]: CP000153); Wolinella succinogenes strain DSM 1740 [56]; Helicobacter hepaticus strain ATCC 51449 [57]; three strains of H. pylori ([58]-[60]: 26695, J99 and HPAG1, respectively); Helicobacter acinonychis strain Sheeba [61]; and the deep-sea vent taxa Nitratiruptor sp. and Sulfurovum sp. (strains SB155-2 and NBC37-1, respectively [62]). This study presents the genomic sequence of a human clinical isolate, A. butzleri strain RM4018, a derivative of the type strain ATCC 49616. The genomic data revealed multiple differences between A. butzleri and other members of the Campylobacteraceae, as well as pathways and systems vital for its survival in diverse environments.
Results and Discussion
General features
The genome of Arcobacter butzleri strain RM4018 contains 2,341,251 bp; as such it is the second largest characterized epsilonproteobacterial genome to date, smaller than the genome of Sulfurovum strain NBC37-1 (2,562,277 bp) but larger than both the genomes of S. denitrificans strain ATCC 33889 (2,201,561 bp) and W. succinogenes strain DSM 1740 (2,110,355 bp). The G+C content of the RM4018 genome (27%) is remarkably low. A summary of the features of the strain RM4018 genome is provided in Table 1. A diagrammatic representation of the RM4018 genome is presented in Figure S1.
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Table 1. Features of the Arcobacter butzleri RM4018 genome
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Consistent with its size, the RM4018 genome is predicted to encode 2259 coding sequences (CDSs). Based on pairwise BLASTP comparisons of proteins predicted to be encoded by these CDSs against proteins in the NCBI non-redundant (nr) database (release 10/13/2007), and on the presence of various Pfam and PROSITE motifs, 1011 (45%) of the predicted proteins were assigned a specific function, 505 (22%) were attributed only a general function, and 743 (33%) were considered proteins of unknown function (Table 1). A complete list of the CDSs predicted to be present within the genome of strain RM4018 and their annotation is presented in the supplementary Table S1. A breakdown of the CDSs by function is presented in supplementary Table S2.
Relationship of A. butzleri to other taxa
Arcobacter butzleri is a member of the family Campylobacteraceae which includes also the genera Campylobacter and Sulfurospirillum. Given the close taxonomic relationship between Arcobacter and Campylobacter, it is noteworthy that 17.2% and 12.4% of the RM4018 proteins have their best match in proteins encoded by S. denitrificans and W. succinogenes, respectively, both members of the Helicobacteraceae (Table 2). Moreover, approximately 25% of the RM4018 proteins have their best match in proteins encoded by Sulfurovum or Nitratiruptor, deep-sea vent Epsilonproteobacteria isolated from a sulfide mound off the coast of Japan [62].The percentage of S. denitrificans homologs with best matches is greater than that of the eight sequenced Campylobacteraceae species combined (13.1%). These differences are reduced somewhat by comparing the top five matches and not just the best match; however, even using these parameters, S. denitrificans (9.6%) remains the closest related organism (Table 2). Among the Campylobacters, C. fetus (3.1% best matches) is the closest to A. butzleri, although it is possible that other Campylobacters, whose genomes are as yet un-sequenced, may have higher degrees of similarity to A. butzleri than C. fetus.
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Table 2. Similarity of predicted A. butzleri proteins to proteins from other taxa.
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In the entire A. butzleri strain RM4018 proteome (unique and non-unique), 61.5% (1390/2259) of the proteins have their best matches in proteins encoded by the epsilonproteobacterial taxa, and 79.0% (1785/2259) are similar to Proteobacterial proteins. Most of the matches to non-Epsilonproteobacteria are found within the gamma subdivision (Table 2), in genera such as Marinobacter (Alteromonadales), Oceanospirillum (Oceanospirillales) and Pseudomonas (Pseudomonadales). Other phyla with a moderate number of protein matches include Firmicutes (2.5%), especially Clostridiales (1.5%), and Bacteroidetes/Chlorobi (2.8%). Among the 2259 predicted CDSs of strain RM4018, 315 (13.9%) had no homolog within the nr database using a minimum expect (E) value of 1×10−5, a minimum identity of 25% and a minimum alignment length of 75% (Figure 1, Table 2, Table S3).
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Figure 1. Genome BLAST atlas of the A. butzleri strain RM4018.
Arcobacter butzleri strain RM4018 is the reference genome and is compared to a set of 15 other epsilonproteobacterial genomes, including different Campylobacter (rings 7–14 from center) and Helicobacter (rings 15–17 from center) strains, as well as the UniProt database (outermost ring in black). A web-based “zoomable atlas” can be found at [147].
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Although approximately 30% of the A. butzleri RM4018 proteins with homologs within the nr database have their best matches to non-epsilonproteobacterial proteins, the approximately 550 genes encoding these proteins are not distributed randomly through the RM4018 genome, but rather clustered with respect to position and protein function (Table S2, Table S3). Interestingly, many of these clusters contain proteins involved in transport, and several contain discrete loci associated with a single function, such as the urease and quinohemoprotein loci described below. The genes contained in these loci are found often in the same order as in other taxa, suggesting lateral transfer. However, the G+C content of these gene clusters is not significantly different from the genome as a whole, suggesting that if these clusters were acquired from other non-Arcobacter taxa the acquisition was not recent.
Generally, strain RM4018 proteins involved in “housekeeping” functions (e.g. amino acid biosynthesis, fatty acid biosynthesis and protein synthesis) have homologs well-conserved among the other Epsilonproteobacteria (Table 3). Nevertheless, a number of other major functional categories are more divergent. For example, 19% (13/70) of the strain RM4018 proteins involved in DNA replication and repair have either no homologs or homologs of low similarity (≤ 35% identity) among the other epsilonproteobacterial taxa; this proportion increases to 37% (26/70) if proteins with homologs only within the Campylobacteraceae are included (Table 3). Other divergent functional categories include: sulfur/nitrogen metabolism (34%), transcriptional regulators/σ-factors (47%), signal transduction (49%), cell envelope (37%), chemotaxis (70%) and antibiotic resistance (36%) (Table 3). These divergent functional categories will be discussed in further detail.
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Table 3. Divergence within the major functional categories
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Methyl-directed mismatch repair and the absence of polynucleotide G:C tracts
Members of the Epsilonproteobacteria lack multiple genes in the methyl-directed mismatch repair system (MMR). The MMR system depends upon the presence of three main functions: 1) the MutSLH endonuclease complex, 2) a methylation system to identify parental vs. daughter strands and 3) multiple 5′→3′ and 3′→5′ single-strand DNA exonucleases, e.g. RecJ, ExoI and ExoVII [63]. The A. butzleri RM4018 genome is not predicted to encode MutL or MutH. However, it is predicted to encode a MutS2 family MutS protein, which is distinguished from MutS1 by the presence of a C-terminal Smr domain. It has been proposed that the Smr domain has a MutH-like nicking endonuclease function [64]. Thus, the A. butzleri MutS2 protein may contain both MutS and MutH domains, obviating the need for a MutL scaffold protein. A. butzleri strain RM4018 is not predicted to encode a Dam DNA-adenine methyltransferase (data not shown) used commonly in MMR systems, but the presence of a Dcm DNA-cytosine methyltransferase was demonstrated experimentally in strain RM4018 (data not shown); this Dcm function may be used by the A. butzleri MMR system to distinguish between parental and daughter strands. Although the annotated epsilonproteobacterial genomes contain the 5′→3′ exonuclease RecJ, they do not contain the 3′→5′ exonucleases ExoI or ExoVII. A 32-fold increase in +1 frameshifts and an 11-fold increase in -1 frameshifts were observed in an E. coli ExoI− ExoVII− mutant [65]; therefore, one possible outcome of such a defect in MMR systems would be the formation and extension of hypervariable G:C tracts, such as those identified in all characterized Campylobacter and Helicobacter genomes. A major distinguishing characteristic of the A. butzleri RM4018 genome is the lack of such G:C tracts, a feature shared by the S. denitrificans and W. succinogenes genomes. A comparison of these three genomes revealed the presence of two to four members of the DnaQ superfamily, to which the 3′→5′ exonuclease ExoX belongs [66]. Thus, one or more of these DnaQ homologs may provide the missing 3′→5′ exonuclease function, and it is conceivable that the absence of hypervariable G:C tracts in A. butzleri strain RM4018 and the presence of these tracts in Campylobacter and Helicobacter may be due to the presence or absence of a functional MMR system, respectively.
Sulfur assimilation, oxidation and the biosynthesis of sulfur-containing amino acids
Arcobacter butzleri strain RM4018 contains a number of genes required for sulfur uptake and assimilation (Figure 2). These genes include those encoding the sulfate ABC transporter CysATW, the sulfate binding protein Sbp, the ATP sulfhydrylase CysDN, the adenosine phosphosulfate (APS) reductase CysH, the sulfite reductase proteins CysI and CysJ and the siroheme synthase CysG. CysD and CysN have been identified also in C. coli, but the position of these genes, along with the APS kinase-encoding gene cysC and the 3′(2′),5′-bisphosphate nucleotidase-encoding gene cysQ, is in the capsular locus, suggesting that these genes in C. coli are involved in the formation or modification of the capsule and not sulfur assimilation per se.
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Figure 2. Sulfur assimilation and biosynthesis of the sulfur-containing amino acids.
Genes/proteins in strain RM4018 unique within Campylobacteraceae or proteins with Campylobacteraceae orthologs of low similarity are labeled/shaded in blue.
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Intracellular sulfate is reduced to sulfite in bacteria by one of two pathways. Both pathways first convert sulfate to APS via the ATP sulfhydrylase CysDN [67]. The first pathway converts APS to PAPS (phosphoadenosine phosphosulfate) using the kinase CysC, and then reduces PAPS to sulfite using the PAPS reductase CysH [67]; PAPS toxicity in some taxa is decreased through the conversion of PAPS to APS via CysQ [68], [69]. The second pathway, identified originally in plants and subsequently in taxa such as P. aeruginosa [70] and M. tuberculosis [71], reduces APS to sulfite directly using the APS reductase CysH. The presence of conserved two-cysteine motifs in strain RM4018 CysH, characteristic of bacterial APS reductases [70], [71], and the absence of both CysC and CysQ homologs, suggests that sulfate reduction to sulfite in this strain does not use a PAPS intermediate.
The sulfite generated by strain RM4018 CysH has two potential fates: reduction to sulfide and assimilation into the sulfur-containing amino acids L-cysteine and L-methionine (Figure 2), or oxidation to sulfate, facilitated by a cytochrome c multienzyme complex encoded by the sox genes (ab0563-ab0570). Initial data indicated that sulfite is oxidized by strain RM4018 (data not shown), and also that it can grow in minimal media without added cysteine or methionine (data not shown). Hence, both metabolic fates are possible, and the genetic switch modulating oxidation or reduction of sulfite remains to be identified. Homologs of the sox genes have been identified in multiple taxa, including Paracoccus pantotrophus [72], [73], Chlorobium tepidum [74] and Rhodovulum sulfidophilum [75]. The Sox clusters of some organisms can contain as many as 15 genes, but seven genes (soxXYZABCD) are essential for sulfur oxidation [76]. These seven genes encode four proteins: the heterodimeric c-type cytochrome SoxXA, the heterodimeric sulfur-binding protein SoxYZ, the heterotetrameric SoxCD sulfur dehydrogenase and the thiol sulfate esterase SoxB [76]. Sulfite, sulfide, sulfur and thiosulfate are possible substrates for the Sox complex; thiosulfate and sulfide oxidation requires the entire complex, while sulfite oxidation does not require SoxCD [76]. The presence of SoxCD in strain RM4018 would suggest that thiosulfate is oxidized by this organism. However, other sox genes important for thiosulfate oxidation, e.g. soxV [77], [78], are absent in this strain, as well as the Sbp-related thiosulfate-binding protein CysP. Thus, it is possible that only sulfite and sulfide are oxidized in strain RM4018. Sox proteins are present also in the related epsilonproteobacterial taxa Sulfurovum, Nitratiruptor and S. denitrificans (Table S2). However, there are multiple differences between the sulfur oxidation systems of these related organisms and those of strain RM4018: 1) the sox genes in strain RM4018 form a single cluster instead of two clusters, 2) the strain RM4018 sox cluster contains only one copy of soxY and soxZ and 3) no significant similarity exists between the SoxXA protein of strain RM4018 and the SoxXA proteins of Sulfurovum, Nitratiruptor and S. denitrificans. The sox cluster of strain RM4018 was not detected in 12 additional A. butzleri strains (see below). However, a cluster of sox genes similar to that of strain RM4018 has been identified in A. halophilus (data not shown). Thus, it appears that strain RM4018 may have acquired the sox cluster through lateral gene transfer from another Arcobacter species and that the sulfur oxidation machinery of Arcobacter and, e.g., Sulfurovum are likely to be distinct evolutionarily.
The genome of strain RM4018 contains all of the genes necessary for the biosynthesis of L-cysteine, L-methionine and iron-sulfur clusters (Figure 2). Consistent with the proposed absence of thiosulfate metabolism, the bacterium has only a gene encoding the CysK cysteine synthase A and not the CysM cysteine synthase B that utilizes thiosulfate instead of sulfide to produce S-sulfocysteine [67]. Strain RM4018 also contains both the cobalamin-dependent and cobalamin-independent homocysteine transmethylases MetH and MetE.
The central metabolism of A. butzleri
The general function of the citric acid/tricarboxylic acid (TCA) cycle is to oxidize organic tri- and di-carboxylic acids to provide energy and biosynthetic precursors for metabolism. Arcobacter butzleri strain RM4018 encodes several proteins homologous to other epsilonproteobacterial TCA cycle enzymes (Figure 3), e.g. isocitrate dehydrogenase (AB1321), 2-oxoglutarate dehydrogenase (AB0852-AB0855), malate dehydrogenase (AB1322) and citrate synthase (AB0307). However, A. butzleri strain RM4018 is predicted putatively to encode two aconitate hydratases and two fumarate dehydratases. Additionally, enzymes that catalyze two TCA cycle steps are absent apparently in strain RM4018.
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Figure 3. Predicted TCA, methylcitrate and citramalate cycles of A. butzleri strain RM4018.
Genes unique within the Campylobacteraceae are labeled in green. Genes in parentheses encode multi-subunit proteins or a protein and its cognate accessory protein. 1: pyruvate dehydrogenase; 2: L-lactate dehydrogenase; 3: phosphate transacetylase; 4: acetate kinase; 5: citrate synthase; 6: aconitase; 7: isocitrate dehydrogenase; 8: 2-oxoglutarate:acceptor oxidoreductase; 9: fumarate reductase; 10: fumarase; 11: malate dehydrogenase; 12: malate:quinone oxidoreductase; 13: 2-methylcitrate synthase; 14: 2-methylcitrate dehydratase; 15: aconitase; 16: 2-methylisocitrate lyase; 17: malic enzyme; 18: malyl-CoA hydrolase; 19: malyl-CoA lyase; 20: β-methylmalyl-CoA lyase; 21: mesaconyl-CoA hydratase; 22: mesaconyl-CoA synthetase; 23: citramalate hydrolase; 24: citramalate lyase.
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The genes ab0275 and ab1447 encode proteins homologous to the E. coli aconitate hydratases AcnA and AcnB, respectively. Arcobacter butzleri AcnB shares between 62% and 76% identity with other epsilonproteobacterial aconitases. Arcobacter butzleri AcnD, encoded by ab0275, does not have significant similarity with any of the known epsilonproteobacterial proteins, but has an 82% similarity with the PrpD 2-methylisocitrate dehydratase of Alkaliliminicola ehrlichei as well as 68% similarity with E. coli AcnA. In addition, the proximity of ab0275 to genes that encode the methylcitrate pathway enzymes PrpB and PrpC suggests that in strain RM4018 AcnD is the aconitase of the methylcitrate pathway and AcnB is the aconitase of the TCA cycle. Also, the absence of the 2-methylcitrate dehydratase PrpD and the presence of acnD next to ab0276, which encodes the AcnD-associated protein PrpF, suggests that A. butzleri strain RM4018 contains the alternate AcnD/PrpF methylcitrate pathway [79].
Escherichia coli can express three fumarases: A, B and C. The first two contain iron-sulfur clusters and are unstable aerobically; fumarase C is stable in the presence of oxygen. The genome of strain RM4018 is predicted to encode two fumarases: AB0722 and AB1921. AB0722 has 74% similarity to E. coli fumarase C and would be predicted to be active under aerobic conditions. AB1921 has 48% similarity to E. coli fumarases A and B and would require microaerobic or anaerobic conditions. AB1921 also has 55–64% similarities with two proteins encoded by the obligate microaerophile W. succinogenes and two encoded by H. hepaticus. Interestingly, the sequence similarities with the W. succinogenes proteins WS1766 and WS1767, and the H. hepaticus proteins HH1702 and HH1793 correspond to the first 282 and the last 185 residues of AB1921, respectively, suggesting that the two polypeptides in W. succinogenes or H. hepaticus are fused into one protein in A. butzleri.
No genes encoding the SucCD succinyl-CoA synthetase or the SdhABCD succinate dehydrogenase have been identified in the A. butzleri genome, but the gene cluster ab0296-ab0298 encodes proteins with high similarities (71–85%) to the fumarate reductase FrdABC of C. jejuni, H. hepaticus, H. pylori, and W. succinogenes, which catalyzes the reaction in the reductive direction converting fumarate to succinate. Some bacteria such as E. coli and C. jejuni have genes encoding both Sdh and Frd, and others, e.g. H. pylori and W. succinogenes, encode only Frd. The similar structures of Sdh and Frd preclude predicting, solely from sequence analyses, whether an enzyme is one or the other. Regulation of transcriptional levels by the oxygen content in the atmosphere permits differentiation between both enzymes; obligate aerobes encode Sdh and anaerobes encode Frd. Initial experiments with A. butzleri indicated the presence of fumarate reduction and no succinate oxidation, and this activity increased several-fold in bacteria grown under anaerobic conditions relative to bacteria grown under aerobic conditions (data not shown). These results provided evidence supporting the identification of A. butzleri FrdABC (ab0296-ab0298) as a fumarate reductase (Figure 3).
The genome of strain RM4018 may contain also a pathway, encoded by the gene cluster ab1917-ab1921, which resembles segments of the 3-hydroxypropionate cycle [80], [81] and the citramalate cycle [82], [83], and interconverts glyoxylate and propionyl-CoA with pyruvate and acetyl-CoA (Figure 3). AB1917 and AB1920 are similar to the CitE citrate lyase. In addition, AB1918 is an acetyl-CoA synthetase with a CaiC domain in residues 50-550, and a CitE domain in residues 550-825. CitE domains have strong similarity to malyl-CoA lyases and have lesser homology to malate synthetase domains. Thus, any of these three proteins could function as a malyl-CoA lyase. AB1919 has a ∼73% similarity to enol-CoA hydratase which catalyzes reversible reactions interconverting 2-enoyl-CoA compounds, such as mesaconyl-CoA, and 3-hydroxyacyl-CoA compounds, such as β-methylmalate-CoA. If the acetyl-CoA synthetase encoded by ab1918 and the anaerobic fumarate hydratase encoded by ab1921 were broad specificity enzymes, the former could catalyze the synthesis of mesaconyl-CoA from mesaconate, and the latter the interconversion of mesaconate and citramalate. Finally, citramalate could be synthesized from pyruvate and acetyl-CoA by one of the CitE enzymes. The gene ab1069 encodes an acyl-CoA thioester hydrolase which can catalyze the synthesis of malyl-CoA from malate or the reverse reaction. One of the three citrate lyases mentioned could then convert malyl-CoA to glyoxylate and acetyl-CoA and glyoxylate (Figure 3).
Arcobacter butzleri does not grow on acetate, citrate, propionate or acetate with propionate, and it grows on fumarate, lactate, malate and pyruvate (data not shown). These data suggested that the methylcitrate pathway would function to produce oxaloacetate for the TCA cycle and propionyl-CoA. The latter metabolite together with glyoxylate synthesized from malate via malyl-CoA would be converted to acetyl-CoA and pyruvate. Acetyl-CoA could be synthesized also from pyruvate via the pyruvate dehydrogenase complex encoded by ab1480-ab1482. Alternatively, the methylcitrate pathway could have a regulatory role converting any excess propionyl-CoA to succinate and pyruvate. Through the activity of a malic enzyme encoded by ab1083, pyruvate can be carboxylated to malate and used to replenish the TCA cycle.
Anaerobic and aerobic respiration
A. butzleri has a full complement of genes for aerobic/microaerobic respiration including those encoding NADH:quinone oxidoreductase, ubiquinol cytochrome c oxidase, ferredoxin, cytochrome bd oxidase, cytochrome c oxidase (cbb3-type), and F1/F0 ATPase (Figure 4), but it also has limited ability for anaerobic respiration. Potential electron donors, in addition to NADH, are hydrogen, malate and formate. One large gene cluster encodes three FeNi hydrogenases with one uptake hydrogenase (hupSL) and two membrane associated proteins encoded by hydABCDF and hyaABCD, the latter two predicted to be anchored to the membrane by the b-type cytochromes HydC and HyaC, respectively. There are two formate dehydrogenases, one selenocysteine homolog and one cysteine homolog, suggesting that selenium may be important in their regulation. Also present is a malate:quinone oxidoreductase (Mqo) and a putative lactate dehydrogenase (AB0728), suggesting that lactate may be a potential electron donor. Electrons may be transferred also to the menaquinone pool through the Sox system described above, the number of electrons depending on the substrate oxidized, 2 e− for sulfite and 8 e− for sulfide.
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Figure 4. Respiratory pathways in A. butzleri strain RM4018.
Transfer of electrons to the menaquinone (MQ) pool is represented by blue arrows; transfer of electrons from the menaquinone pool is represented by red arrows. b- and c-type cytochromes are shaded yellow. Genes unique within the Campylobacteraceae are labeled in green. NADH:Q OR: NADH:quinone oxidoreductase; M:Q OR: malate:quinone oxidoreductase; FormDH: formate dehydrogenase; LacDH: L-lactate dehydrogenase; SulfDH: sulfur dehydrogenase; Ubi cyt c OR: Ubiquinol cytochrome c oxidoreductase; TMAO RD: TMAO reductase; NO3RD: nitrate reductase; NO2RD: nitrite reductase; FumRD: fumarate reductase; QHAmDH: quinohemoprotein amine dehydrogenase; AldDH: aldehyde dehydrogenase; PX: peroxidase; CTQ: cysteine tryptophylquinone.
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Fumarate, nitrate and nitrite are used by strain RM4018 as electron acceptors. Fumarate can be reduced to succinate by the fumarate reductase FrdABC. Nitrate can be reduced to ammonia via a periplasmic nitrate reductase NapAB and a pentaheme nitrite reductase NrfAH. The nap operon has the same gene number and order as that seen in Campylobacter species. It is possible also that trimethylamine–oxide (TMAO) and/or dimethylsulfoxide (DMSO) may serve as alternative electron acceptors. Analysis of the strain RM4018 genome indicates the presence of the pentaheme c-type cytochrome TorC encoded by ab1150. The CDS ab1151 has been annotated as bisC, but the BisC family includes also other anaerobic dehydrogenases, such as the TMAO and DMSO reductases TorA and DmsA, respectively. The genes torC and torA are co-transcribed usually with torD, but no TorD homolog was detected in the strain RM4018 genome. Other electron acceptors may be present: CDS ab1360 encodes a putative cytochrome b-type nitric oxide reductase, and ab1987 encodes a putative nitrite/nitric oxide reductase. Additional investigations will be necessary to determine the function of these putative electron acceptors.
In addition to the respiratory proteins described, A. butzleri strain RM4018 is predicted to encode a quinohemoprotein amine dehydrogenase (QHAmDH), the presence of which is novel in the epsilon subdivision. QHAmDH is a heterotrimeric (αβγ) protein that deaminates oxidatively a variety of aliphatic and aromatic amines, e.g. n-butylamine and benzylamine [84], and is unusual in that the small (γ) subunit contains an intrinsic quinone cofactor [in A. butzleri, cysteine tryptophylquinone (CTQ)], formed by the covalent linkage of a cysteine residue to an oxidized tryptophan residue [85]. The presence of this intrinsic quinone cofactor permits the transfer of electrons directly to the cbb3 oxidase through either a cytochrome c [86] or blue-copper protein (e.g. azurin [87]) intermediate (Figure 4). The QHAmDH locus of strain RM4018 encodes also a radical SAM (S-Ado-Met) family protein (AB0466). A radical SAM protein (ORF2) is found also in the QHAmDH locus of Paracoccus denitrificans and has been proposed to play an important role in the formation of the CTQ cofactor [88]. Amino acid motifs conserved in ORF2 are 100% identical to those in AB0466, as well as to the relevant amino acids in the γ subunit AB0467, suggesting that the strain RM4018 QHAmDH locus encodes a functional dehydrogenase. M9 minimal media amended with 0.5% n-butylamine-HCl (v/v), benzylamine-HCl (w/v) or methylamine-HCl (w/v) as a sole carbon source does not support the growth of strain RM4018 (data not shown). However, substrate specificity has been demonstrated among the amine-utilizing taxa [89]; therefore, it is likely that RM4018 utilizes an as yet unidentified aliphatic or aromatic amine. Finally, two aldehyde dehydrogenases, encoded putatively by ab0376 and ab2135, also novel in the Epsilonproteobacteria, were identified in strain RM4018 (Figure 4). Thus, it is possible that the aldehydes generated from the QHAmDH are oxidized further.
Urease
Arcobacter butzleri strain RM4018 contains six genes (ab0808-ab0813) involved in the degradation of urea. In bacteria this catabolism involves generally three sets of genes: a nickel-containing urease (composed of α, β and γ subunits), urease accessory proteins which deliver the nickel to the urease and a nickel uptake system. The urease of strain RM4018 is functional (data not shown), as determined by a phenol red/urea assay [90], although the level of activity is not as high as that found in other urease-producing taxa (e.g. UPTC C. lari). As in Helicobacter, the urease α and β subunits are fused, and the strain RM4018 urease subunits show high homology to Helicobacter urease subunits. However, differences exist with the urease loci of both Helicobacter and UPTC C. lari. First, the gene order of the locus itself, ureD(AB)CEFG, is similar to the gene order of the urease loci in Klebsiella, Proteus, E. coli O157:H7 and Vibrio, but not the Helicobacter locus ure(AB)IEFGH. Second, although the urease enzyme itself is similar to the Helicobacter urease, the accessory proteins UreD, UreE and UreF, had greater similarity to those identified in Bacillus, Lactobacillus and Psychromonas (Table S3); additionally, the nickel-binding protein UreE has a histidine-rich C terminus, found in multiple UreE proteins, but not in those from Helicobacter or C. lari. Also, unlike Helicobacter, no obvious nickel uptake system, such as the H. hepaticus nikABDE or H. pylori nixA, was found in strain RM4018. A putative nickel transporter, AB1752, was identified, but it is unclear whether it is specific for nickel, or is a heavy-metal-ion transporter. Finally, although the A. butzleri urease may serve to degrade exogenous urea, it may also degrade endogenous urea, formed during putrescine biosynthesis, specifically during the conversion of agmatine to putrescine by SpeB (AB1578).
Surface structures
SDS-PAGE analysis suggested that A. butzleri strain RM4018 can express lipooligosaccharide (LOS). To date, little is known about the roles of these molecules in A. butzleri. They generate great attention in bacterial pathogens since LOS/lipopolysaccharide (LPS) are major inducers of proinflammatory responses, are immunodominant antigens, and play a role in host cell interactions. The LOS biosynthesis locus of strain RM4018 (ab1805-ab1833) showed a similar organization to those of Campylobacter [51], [52], [91], [92], and is thus dissimilar from the loci of Helicobacter and Wolinella. At both ends of the locus are genes involved in the addition of heptose to the oligosaccharides and the encoded proteins are similar to those of other Epsilonproteobacteria (Table S2). Within the locus, genes are found whose products have functions related to LOS/LPS biosynthesis, including several glycosyltransferases, but the proteins encoded have greater similarity to proteins outside of the Epsilonproteobacteria (Table S3). This LOS/LPS biosynthesis region is conserved among 12 unrelated A. butzleri strains, based on comparative genomic indexing (described in detail later), which likely distinguishes A. butzleri from C. jejuni, where the LOS biosynthesis region is an intraspecies hypervariability region [93]. The gene conservation of this region in A. butzleri resembles more closely the conservation of the LPS core biosynthesis region occurring among many of the Salmonella enterica serovars [94].
Many bacteria synthesize structurally diverse polysaccharide polymers, O-antigen and capsule that are major antigenic determinants. It is possible that A. butzleri strain RM4018 produces O-antigen, since there is a locus (ab0661-ab0697) that encodes several additional glycosyltransferases. This region has two copies of wbpG, hisH, and hisF, found also in the Pseudomonas aeruginosa B-band O-antigen locus [95], and many of the other encoded proteins have greater similarity to proteins from bacteria outside of the Epsilonproteobacteria (Table S2). Although this region could be involved putatively in capsular formation, the absence of conserved kps capsular genes in this region, combined with the presence of O-antigen-related genes, suggests that A. butzleri strain RM4018 produces O-antigen and not capsule; however, further investigations will be necessary to determine the nature of the A. butzleri cell-surface structures. Like many O-antigen biosynthesis regions, the A. butzleri region appears to represent an intraspecies hypervariability region with the RM4018 region, present in only 1 of the 12 A. butzleri strains examined using comparative genomic indexing.
Arcobacter butzleri strain RM4018 is a motile bacterium that synthesizes a polar flagellum. Many of the flagellar apparatus proteins encoded by strain RM4018 have homologs in other epsilonproteobacterial taxa. However, phylogenetic analysis of selected flagellar proteins suggests that the flagellar apparatus of strain RM4018 has an evolutionary history distinct from those of Campylobacter and Helicobacter (Figure 5A). This distinct history is supported also by predicted differences between strain RM4018 and Campylobacter/Helicobacter in flagellar gene regulation (see below). Additionally, the flagellar genes of strain RM4018 are highly clustered, compared to the flagellar genes of Campylobacter jejuni (Figure 5B). The primary cluster in strain RM4018 contains 20 flagellar genes (ab1931-ab1961) with the other two flagellar clusters containing eight (ab0197-ab0208) and three (ab2238-ab2244) genes. The flagellar genes of the related organism Nitratiruptor are also highly clustered with the primary cluster containing 36 flagellar and chemotaxis genes. Significantly, the Nitratiruptor flagellar proteins also appear to be distinct phylogenetically from those of both A. butzleri and Campylobacter/Helicobacter. Moreover, the primary flagellar cluster of Nitratiruptor has an atypical G+C content, suggesting acquisition through horizontal gene transfer [62]. Although the G+C content of the strain RM4018 flagellar genes is not atypical, it is possible that the flagellar genes of this organism were acquired via a similar mechanism.
[Figure omitted. See PDF.]
Figure 5. Phylogenetic analysis of four representative flagellar proteins and flagellar clustering in strain RM4018.
A. Each dendrogram was constructed using the neighbor-joining algorithm and the Kimura two-parameter distance estimation method. Bootstrap values of >75%, generated from 500 replicates, are shown at the nodes. The scale bar represents substitutions per site. Cjj: Campylobacter jejuni subsp. jejuni; Cjd: C. jejuni subsp. doylei; Cc: C. coli; Cu: C. upsaliensis; Cl: C. lari; Cff: C. fetus subsp. fetus; Ccur: C. curvus; Ccon: C. concisus; Ab: A. butzleri; Ws: W. succinogenes; Sd: S. denitrificans; Hh: H. hepaticus; Hac: H. acinonychis; Hp: H. pylori; Nit: Nitratiruptor sp.; Ec: Escherichia coli. B. Location of the flagellar genes of C. jejuni strain RM1221 and A. butzleri strain RM4018.
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Prophage and genomic islands
The genome of the A. butzleri strain RM4018 is predicted to contain a prophage. The size of this prophage is approximately 38 kb and spans genes ab1655-ab1706. BLASTP comparison of the predicted phage proteins to proteins from other bacteriophage indicates that this prophage is a member of the mutator (Mu) bacteriophage family. The size of the prophage is similar to other Mu-like bacteriophage, and it contains proteins similar to the Mu transposition proteins A and B in addition to coat, baseplate, and tail proteins. Mu-like bacteriophage have been found in multiple bacterial taxa, including E. coli, Neisseria meningitidis, Deinococcus radiodurans, Haemophilus influenzae, Burkholderia cenocepacia [96], [97] and notably C. jejuni (CMLP1: strain RM1221[52], [93]). Indeed, 26 of the 50 predicted RM4018 Mu-like phage proteins are similar to those encoded by CMLP1 (Table S2). Hence, it is proposed to name this bacteriophage AMLP1 (Arcobacter Mu-like phage). Mu-like bacteriophage have been identified in other epsilonproteobacterial taxa, including Campylobacter (11 species), Arcobacter (3 species), and Helicobacter bilis ([93]; Miller and Mendoza, unpublished data). Some of these Mu-like phage are similar to CMLP1, but many show marked variation in gene content and gene sequence, indicating that CMLP1 and AMLP1 are members of a diverse Mu bacteriophage family common to Proteobacteria.
The genome of strain RM4018 also contains three small genomic islands, termed ABGI1, ABGI2 and ABGI3 (for Arcobacter butzleri genomic island). ABGI1 is 26,918 bp (bp 1,324,568-1,351,485) and contains 29 genes (ab1330-ab1358). ABGI2 is 15,973 bp (bp 1,703,320-1,719,292) and contains 13 genes (ab1721-ab1733). ABGI3 is 4,907 bp (bp 2,103,976-2,108,882) and contains eight genes (ab2090-ab2097). Additionally, ABGI1-3 are bordered by direct repeats of 21, 21 and 25 bp, respectively. The presence of genomic islands within the epsilon subdivision is not unusual. They have been identified in C. jejuni (CJIE2 and CJIE3; [52]), H. hepaticus (HHGI; [57]) and H. pylori (cagPAI; [58]). CJIE2 and CJIE3 are bounded by direct repeats, contain an integrase gene at one end, and have integrated into the 3′ end of a tRNA [52], [93]. Consistent with the C. jejuni islands, all three RM4018 genomic islands contain terminal integrase genes and ABGI1 has inserted into the 3′ end of a leucinyl-tRNA; however, ABGI2 and ABGI3 did not integrate into tRNAs, although ABGI3 is in close proximity to a cysteinyl-tRNA. CJIE3 has been proposed to be an integrated plasmid, based on the similarity of this element to the C. coli strain RM2228 megaplasmid [52], [93]; in contrast, none of the protein functions encoded by elements ABGI1-3 suggested a plasmid origin. Interestingly, ABGI2 does encode proteins similar to the Type I restriction enzymes HsdS and HsdM. Thus, the only Type I, II, or III restriction/modification enzymes present in strain RM4018 are encoded by a genomic island. The role of ABGI3 is unknown.
Antibiotic resistance
Arcobacter butzleri strain RM4018 was resistant to 42 of the 65 antibiotics tested (see supplementary Table S4). This level of resistance is remarkably high, considering that the multi-drug-resistant C. coli strain RM2228 was resistant to only 33 of the same 65 antibiotics ([52] and Table S4). Strain RM4018 was resistant to all macrolides and sulfonamides tested and to all of the β-lactam antibiotics, with the exception of the β-lactam cephalosporin ceftazidime. Strain RM4018 had resistance to some quinolones, i.e. nalidixic acid and oxolinic acid, and also to chloramphenicol and 5-fluorouracil (5FU). The pattern of antibiotic resistance in strain RM4018 is consistent, in part, with the resistances of 39 A. butzleri strains tested against a smaller set of 23 antibiotics by Atabay and Aydin [98], with the exception that strain RM4018 was resistant to chloramphenicol and nalidixic acid. No plasmids were detected in strain RM4018; therefore all resistance mechanisms would be chromosomal in nature.
In many cases, the antibiotic resistance observed for strain RM4018 was due to the presence or absence of genes characterized previously in terms of antibiotic resistance. For example, chloramphenicol resistance is due most likely to the presence of a cat gene (ab0785), which encodes a chloramphenicol O-acetyltransferase. β-lactam resistance is due probably to the three putative β-lactamases (AB0578, AB1306 and AB1486) present in the RM4018 genome; β-lactam resistance may be enhanced also by the presence of the lrgAB operon (ab0179, ab0180) which modulates penicillin tolerance in Staphylococcus [99], [100]. Uracil phosphoribosyltransferase, encoded by the upp gene, catalyzes the first step in the pathway that leads to the production of the toxic analog 5-fluorodeoxyuridine monophosphate; mutations in the upp gene have been shown to lead to increased 5FU resistance [101], [102]. Thus, the absence of upp in A. butzleri strain RM4018 results presumably in high 5FU resistance.
Although strain RM4018 is resistant to some quinolones, mutations implicated previously in Campylobacter [103] and Arcobacter [104] quinolone resistance at Thr-86, Asp-90 and Ala-70 in the DNA gyrase subunit GyrA are not present in strain RM4018. It is probably not a coincidence that strain RM4018 is susceptible to hydrophilic quinolones (e.g. ciprofloxacin and norfloxacin) and resistant to hydrophobic ones (e.g. nalidixic acid and oxolinic acid). These data suggest that the mechanism of quinolone resistance in strain RM4018 is not at the level of the gyrase, but rather of uptake. Decreased quinolone uptake is associated with either increased impermeability or the activity of efflux pumps [105]. Hydrophobic quinolones alone can transit across the phospholipid bilayer but all quinolones can enter the cell through porins [105]. It is possible that the phospholipid bilayer of strain RM4018 has reduced permeability towards hydrophobic quinolones in conjunction with modifications in the porins to permit passage of only hydrophilic quinolones. A more likely scenario is the presence of a hydrophobic quinolone-specific efflux pump. Examples of pumps with specificity towards one class of quinolone are known; for example, the NorA protein of Staphylococcus aureus has been shown to be involved in the specific efflux of hydrophilic quinolones [106].
Virulence determinants
Putative virulence determinants have been identified in Campylobacter, but little is known about potential virulence factors in A. butzleri. Since campylobacterioses and reported A. butzleri-related illnesses have similar clinical outcomes [10], it might be expected that some C. jejuni virulence factors would be found in Arcobacter. In fact, some virulence determinants identified in C. jejuni, have homologs within A. butzleri. For example, the fibronectin binding proteins CadF and Cj1349 have homologs in strain RM4018 (AB0483 and AB0070, respectively). Moreover, homologs of the invasin protein CiaB, the virulence factor MviN, the phospholipase PldA and the TlyA hemolysin are present in strain RM4018 (AB1555, AB0876, AB0859 and AB1846, respectively). However, it has not been determined if these putative virulence determinants are functional or if their function and role in A. butzleri biology is similar to the function of their Campylobacter homologs.
On the other hand, several Campylobacter virulence-associated genes are not present in the RM4018 genome. Most notably, the genes encoding the cytolethal distending toxin CDT (cdtABC) are absent from strain RM4018. CDT is an exotoxin which irreversibly blocks eukaryotic cells in the G1 or G2 phase [107]; cdtABC genes have been identified in Helicobacter hepaticus and several characterized Campylobacter genomes. The absence of cdtABC in strain RM4018 correlated well with a study by Johnson and Murano [108] which was unable to detect cdt genes in Arcobacter by PCR. A. butzleri strain RM4018 also contains no PEB1 or JlpA adhesin homologs [109], [110].
Analysis of the strain RM4018 genome identified two additional putative virulence determinants: irgA (ab0729) and hecAB (ab0941-ab0940). The irgA gene in V. cholerae encodes an iron-regulated outer membrane protein [111], and an IrgA homolog has been demonstrated to play a role in the pathogenesis of urinary tract infections by uropathogenic E. coli [112]. Adjacent to irgA is ab0730, which encodes a putative IroE homolog. IroE is a siderophore esterase found also in uropathogenic E. coli [113]. The other novel virulence determinant, HecA, is a member of the filamentous hemagglutinin (FHA) family, and hecB encodes a related hemolysin activation protein. FHA proteins are distributed widely among both plant and animal pathogens, e.g. HecA in Erwinia crysthanthemi contributes to both attachment and aggregation and is involved in epidermal cell killing [114]. Consistent with this distribution, RM4018 HecA homologs occur in both plant- (Pseudomonas syringae, Ralstonia solanacearum) and animal-pathogens (Burkholderia cepacia, Acinetobacter spp. and uropathogenic E. coli).
Interacting with the environment: chemotaxis and signal transduction
Microbial life is characterized by continuous interactions between bacteria and their environment. The ability of microorganisms to monitor environmental parameters is a prerequisite for survival. Hence, bacteria have evolved different mechanisms such as sensory histidine kinases, methyl-accepting chemotaxis proteins, sigma(σ)/anti-sigma factor pairs, and adenylate and diguanylate cyclases to monitor and rapidly adapt to changes in their environment.
Arcobacter butzleri is well equipped with a large number of these systems, allowing it to survive in diverse ecological niches. Prime bacterial mechanisms of environmental adaptation and gene regulation are the two-component systems (2CS), consisting of a membrane-bound sensor histidine protein kinase (HPK) and a cytoplasmic response regulator (RR). A. butzleri has one of the highest densities of 2CS genes per megabase, with a total of 78 genes of this type (Table S2). Analysis of the genome sequence revealed 36 HPK genes, 41 RR genes, and one hybrid HPK/RR gene. On the genome, 31 pairs of HPK and RR genes are present that likely form functional 2CS involved in responses to environmental changes. In three instances, a more complex regulation may exist, where an additional sensor or response regulator gene is adjacent to the pair, namely AB0416, AB0417, AB0418; AB0453, AB0454, AB0455; and AB0795, AB0796, AB0797.
A fully functional chemotaxis system modulated by the 2CS CheA-CheY is present in A. butzleri strain RM4018. Its genome encodes a large number of putative chemotaxis proteins, many of which are novel in the epsilon subdivision. A. butzleri is predicted to have the CheA, CheY, CheV, CheW, CheR and CheB core enzymes, although most of these proteins have low similarity to their epsilonproteobacterial orthologs, and some have key differences in terms of domain composition. For instance, CheA in Campylobacter spp. is fused to a CheY-like response regulator (RR) domain [115] which is absent from A. butzleri CheA. Also, the methylesterase CheB in A. butzleri has both the C-terminal methylesterase and N-terminal RR domains whereas the C. jejuni CheB protein contains only the methylesterase domain [115]. The presence of this CheB RR domain may support adaptation in A. butzleri, allowing the cell to “sense” increasing concentrations of attractants. In addition to these core chemotaxis proteins, strain RM4018 is predicted to encode the CheC phosphatase, the CheD glutamine amidase, involved in maturation of the methyl-accepting chemotaxis proteins (MCPs), and two additional CheY proteins. CheC hydrolyzes phosphorylated CheY and is employed by multiple taxa as an alternative to CheZ in signal removal [116]. The additional CheY proteins also could modulate signal removal by serving as a “phosphate sink” [116]. In the chemotaxis network, the receptor and kinase functions are separated in order to allow the cell to execute an integrated response to multiple stimuli [117]. The elaborate sensing capabilities of A. butzleri are reflected also in its high number (N = 25) of chemotaxis-specific receptors (MCPs). Some MCPs, e.g. AB1764, contain Cache (Ca2+ channels and chemotaxis) domains which have been identified in multiple bacterial taxa [118], and are similar to those found in animal voltage-gated Ca2+ channels. In addition, the periplasmic sensor region of AB1496 contains a putative nitrate/nitrite sensory domain, suggesting that nitrate and/or nitrite serves as an attractant for A. butzleri.
The limited similarity between the remaining non-chemotaxis-associated 2CS of A. butzleri and those of other bacteria do not allow functional prediction. However, six histidine kinases possess PAS domains, which are involved often in sensing changes in cellular energy levels, oxygen levels, or redox potential. Remarkable is the apparent lack of genes encoding homologs of the NtrC/NtrB family of two-component proteins that act together with the σ factor RpoN. Genes encoding RpoN as well as the flagellar sigma factor FliA are also missing in the A. butzleri genome. This indicates that the transcriptional regulation of the A. butzleri polar flagella machinery is completely different from that of other Epsilonproteobacteria where the 2CS FlgS/FlgR together with RpoN and FliA regulate the formation of the basal-body hook and filament complex [119], [120]. Regulation of A. butzleri flagella synthesis may involve a completely novel system, since also absent from the genome are genes encoding homologs of the lateral master flagella transcription factors, such as FlhC, FlhD of E. coli [121] or members of the LuxR-type of transcription regulators like Sinorhizobium meliloti VisNR [122].
There are no predicted alternate sigma factors in the A. butzleri genome. Instead, and unlike the genomes of Campylobacter spp. or Helicobacter spp., it contains seven ECF-family (extracytoplasmic function) σ/anti-σ factor pairs. Genes encoding these pairs are in tandem. Anti-σ factors are usually membrane-bound and prevent their cognate σ factor from associating with core RNA polymerase [123]. To date, only the W. succinogenes proteins WS1422 and WS1423 have been identified as an ECF-family σ factor pair within the epsilon subdivision. It is not known what genes are regulated in A. butzleri by these ECF-family σ factors. In other bacterial taxa, these ECF-family σ factors regulate functions such as alginate biosynthesis, iron-citrate transport, pyoverdine biosynthesis and cytochrome expression [123], and have been associated with virulence in S. enterica, P. aeruginosa and M. tuberculosis [124].
Finally, A. butzleri strain RM4018 encodes a class of proteins involved in formation and turnover of the “second messenger” bis-(3′-5′)-cyclic dimeric guanosine monophosphate (cyclic-di-GMP). In bacteria cyclic-di-GMP is synthesized by diguanylate cyclases containing a conserved GGDEF motif (GGDEF(DUF1) domain proteins) and degraded into diguanylate (pGpG) by phosphodiesterase proteins containing domains enriched in glutamic acid, alanine and leucine (EAL(DUF2) domain proteins) [125], [126]. The genome of strain RM4018 encodes 25 proteins, either membrane-associated or cytoplasmic/periplasmic, containing GGDEF and/or EAL domains (Figure S2b). In other bacteria, GGDEF/EAL domain proteins have been implicated in biofilm formation [127], [128], motility [129], [130] and virulence [131].
It is likely that many of the signal transduction proteins described above interact across group boundaries, e.g. GGDEF proteins with chemotaxis proteins. Thus, taken together, the large repertoire of 2C systems, chemotaxis proteins, ECF sigma factors and cyclic-di-GMP factors suggest the presence of a truly complex signal transduction network.
Genomic comparisons with other A. butzleri strains
The genomic diversity of 13 A. butzleri strains, of both human and animal origin, was examined by microarray-based comparative genomic indexing (CGI) analysis. A list of the strains used in the CGI analysis is presented in Table 4. The CGI analysis allowed the assessment of CDS content for each A. butzleri strain relative to the A. butzleri DNA microarray, which comprises 2238 CDS from strain RM4018. Genomic DNA from strain RM4018 was used as a reference DNA and competitively hybridized with genomic DNA from each of the other A. butzleri strains. The GENCOM software described previously [132], [133] was used to assign the CDS as present, absent or multicopy for each A. butzleri strain. The complete CGI data sets as trinary scores (present = 1; absent = 0; and multicopy = 2) are available in supplementary Table S5. It was observed that 74.9% (1676 of 2238) of the CDS represented on the microarray were present in all A. butzleri strains, and served to define approximately the core genes of A. butzleri (supplementary Table S6).
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Table 4. Strains used in the comparative genomic indexing analysis.
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To gain more information concerning how genomic diversity may affect the physiology of these A. butzleri strains, the A. butzleri CDS were grouped into defined functional categories. Genes in each functional category for the A. butzleri RM4018 genome were analyzed against the CGI data set with GeneSpring software. The results show that all A. butzleri strains in this study possessed every gene in our data set assigned to the functional categories: polyamine biosynthesis; purines, pyrimidines, nucleosides and nucleotides biosynthesis; aminoacyl tRNA synthetases and tRNA modification; protein translation and modification; and protein and peptide secretion (Table 5). The results demonstrated also that more than 95% of the genes in our data set assigned to the following functional categories were present for all strains: energy metabolism; amino acid biosynthesis; and ribosomal protein synthesis, modification and maturation (Table 5). In contrast, functional categories with less than 70% of the genes present in at least two strains were: central intermediary metabolism; transcriptional regulation; surface polysaccharides, lipopolysaccharides, and antigens; surface structures; phage-related functions and prophage; and hypothetical proteins (Table 5). There were also 42 CDS that were found only in strain RM4018 (Table S7), including the Sox cluster of seven genes (soxXYZABCD), suggesting that this system was acquired by RM4018. Some genes within the bacteriophage AMLP1 were present in the other 12 A. butzleri strains, but this prophage was not present in its entirety in any of the other 12 A. butzleri strains. The same applies to the small genomic islands ABGI1 and ABGI2. Finally, urea degradation does not appear to be a core function of A. butzleri, considering that only six of the 12 strains possessed the gene clusters ab0802-ab0806 and ab0808-ab0813.
[Figure omitted. See PDF.]
Table 5. Assignment of absent CDS to functional categories of A. butzleri.
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Conclusions
The human pathogen A. butzleri is a member of the family Campylobacteraceae in the epsilon subdivision of the Proteobacteria, and it was found, based on 16S rDNA sequence similarity, to be most closely related to the Campylobacters. However, based on complete genome analysis, A. butzleri appeared more closely related to non-Campylobacteraceae taxa, specifically S. denitrificans, W. succinogenes, Sulfurovum and Nitratiruptor. This unexpected finding may be less surprising considering the limited number of completed epsilonproteobacterial genomes. Considering that to date the Epsilonproteobacteria genome sequences annotated belong in their majority to pathogens, further refinements of the structure of the epsilonproteobacterial taxon are likely to emerge, as additional genomes of this subdivision are sequenced, especially those of other Arcobacter species, and of related genera such as Sulfurospirillum.
Even though A. butzleri has a large number of S. denitrificans, W. succinogenes, Sulfurovum and Nitratiruptor orthologs, strain RM4018 has several features in common with Campylobacter, such as the inability to utilize sugars as carbon sources and the presence of defined LOS/LPS loci. Interesting differences between A. butzleri and the Campylobacters are the absence of Type I, II or III restriction enzymes and the absence of polynucleotide G:C tracts. These tracts in Campylobacter are found often in LOS and capsular loci, and changes in the lengths of these tracts could modify the surface structure of the organism, perhaps altering the serotype. Therefore, the absence of these tracts and the resulting absence of contingency genes may disadvantage A. butzleri as a pathogen. Alternatively, the absence of restriction enzymes may facilitate the uptake and incorporation of phage, genomic islands, and novel genes/loci, as seen in strain RM4018, thus providing the genomic plasticity that is associated often with virulence.
Notwithstanding its relationships to Campylobacter and Helicobacter, A. butzleri is probably an environmental organism that may cause disease through either water-mediated food contamination or ingestion of A. butzleri-contaminated water, and whose biology is not associated tightly with any particular host or hosts. A. butzleri is more psychrophilic than Campylobacter spp. and may not proliferate in some hosts, especially avian hosts, to the same extent as, e.g. C. jejuni, resulting in A. butzleri-related food-borne illnesses occurring less frequently. The enhanced ability to survive in the environment, compared to such taxa as Campylobacter, is due most likely to the genes and systems described in this study, such as the increased number of respiratory enzymes, chemotaxis proteins and two-component systems. A. butzleri has a number of genes that confer increased environmental survival and the ability to grow under a wider range of atmospheric conditions and lower temperatures. An example of the first would be the photoreactivating lyase phr, which reduces DNA damage induced by UV radiation, a condition encountered frequently by environmental organisms. Examples of the second would include: 1) more oxygen-stable enzymes (e.g. pyruvate dehydrogenase), 2) a larger repertoire of TCA cycle enzymes active over a greater range of oxygen concentrations, 3) novel cold shock and stress proteins and 4) proteins such as the fatty acid cis/trans isomerase, Cti, which modulate membrane fluidity.
Although A. butzleri has been isolated from clinical diarrheal stool samples, the association of this organism with human illness is not as defined as the association of C. jejuni and human gastroenteritis. Obviously, the presence of Campylobacter virulence determinant homologs in strain RM4018 is not predictive of the pathogenicity of this strain; however, it is consistent with such a designation. It is likely, if A. butzleri is indeed pathogenic, that additional uncharacterized virulence determinant genes are present within the strain RM4018 genome. Therefore, additional investigations, using in part the genomic information presented here, will be required to elucidate further the mechanisms supporting the pathogenicity of A. butzleri. Indeed, the genome sequence and analyses presented in this study are an important first step in understanding the physiology and genetics of this organism, which constitutes a bridge between the environment and mammalian hosts.
Materials and Methods
Growth conditions and chemicals
All Arcobacter strains were cultured routinely at 28°C on Brain Heart Infusion agar (Becton Dickinson, Sparks, MD) amended with 5% (v/v) laked horse blood (Hema Resource & Supply, Aurora, OR). The incubation atmosphere for all strains was 5% H2, 10% CO2, and 85% N2. PCR enzymes and reagents were purchased from New England Biolabs (Beverly, MA) or Epicentre (Madison, WI). All chemicals were purchased from Sigma-Aldrich Chemicals (St. Louis, MO) or Fisher Scientific (Pittsburgh, PA). DNA sequencing chemicals and capillaries were purchased from Applied Biosystems (Foster City, CA).
Genomic DNA preparation and library construction
A sample of the Arcobacter butzleri type strain ATCC 49616 was analyzed by two methods: 1) an Arcobacter multiplex speciation PCR [134] and 2) amplification and sequencing of the 16S rDNA locus, using the primers 16ARCO-1 (5′ ACA ATG GAC GAA AGT CTG AT 3′) and 16ARCO-2 (5′ CGC AAT CGG TAT TCC TTC 3′). Results of these tests indicated that the type strain isolate was a mixed culture containing an uncharacterized A. cryaerophilus strain. Therefore, a phosphate-buffered saline suspension of the type strain isolate was sonicated and dilution plated. Eight well-isolated colonies were picked and tested using the above two methods. Both tests confirmed that the DNA from each of the eight colonies was from a pure culture of A. butzleri and one purified isolate was given the new designation of RM4018. Moreover, the multilocus sequence type of strain RM4018 was identical to the sequence types of multiple isolates of strain ATCC 49616, obtained from independent laboratories (W. Miller, manuscript in preparation).
Arcobacter genomic DNA was prepared as described previously [135]. The genomic DNA was checked for high molecular weight using pulsed field gel electrophoresis, quantified using a PicoGreen assay (Invitrogen, Carlsbad, CA), and then sheared physically to the desired average sizes of approximately 4.0 kb and 40.0 kb, for the small- and large-fragment libraries, respectively. The 3–4 kb plasmid insert library was constructed in the pAGEN proprietary vector system (Agencourt). A large-insert fosmid library was constructed in the CopyControl pCC1FOS vector system (Epicentre, Madison, WI). Libraries were not amplified and were kept as ligation mixtures.
Initial quality control of each library included quantification of: the titer of each one, the frequency of recombinant/insert-less clones and the average insert size (using a subset of 20 recombinant clones). Prior to high throughput sequencing, 384 clones were picked and sequenced bi-directionally. A BLAST analysis was performed on the 768 sequencing reads to verify the integrity of the library. Sequence data was further reviewed to provide more precise information on the percentage of recombinant clones.
Template preparation
Solid Phase Reversible Immobilization (SPRI) [136], [137] technology was used to purify the templates. SPRI technology uses carboxylate-coated, iron-core, paramagnetic particles to capture DNA of a desired fragment length based on tuned buffering conditions. Once the desired DNA is captured on the particles, they can be concentrated magnetically and separated so that contaminants can be washed away.
High-copy plasmid templates (insert size range 800 bp–4 kb) were purified using a streamlined SprintPrep SPRI protocol. Plasmid DNA was harvested directly from lysed bacterial cultures by trapping both plasmid and genomic DNA with the functionalized bead particles and selectively eluting only the plasmid DNA. Briefly, the purification procedure involves addition of alkaline lysis buffer (containing RNase A) to the bacterial culture, addition of an alcohol-based precipitation reagent including paramagnetic particles, separation of the magnetic particles using custom ring-based magnetic separator plates, 5× washing of the beads with 70% EtOH and elution of the plasmid DNA with water. The fosmid templates were purified using a SPRI protocol optimized to accommodate larger growth volumes and increased sample recovery needed for the low copy nature of these vectors.
Polymerase chain reactions
Standard amplifications were performed on a Tetrad thermocycler (Bio-Rad, Hercules, CA) with the following settings: 30 s at 94°C; 30 s at 53°C; 2 min at 72°C (30 cycles). Each amplification mixture contained 50 ng genomic DNA, 1× PCR buffer (Epicentre), 1× PCR enhancer (Epicentre), 2.5 mM MgCl2, 250 µM each dNTP, 50 pmol each primer, and 1 U polymerase (New England Biolabs). Amplicons were purified on a BioRobot 8000 Workstation (Qiagen, Santa Clarita, CA). Sequencing and PCR oligonucleotides were purchased from Qiagen or MWG-Biotech (High Point, NC).
DNA sequencing
DNA templates from shotgun clones were sequenced in a 384-well format. Cycle sequencing reactions were performed on a 96-well or 384-well Tetrad thermocycler (Bio-Rad, Hercules, CA) using the ABI PRISM BigDye terminator cycle sequencing kit (version 3.1) and standard protocols. Shotgun template extension products were purified using the CleanSeq dye-terminator removal kit (Agencourt) and amplicon extension products were purified using DyeEx 96 well plates (Qiagen) according to the manufacturer's protocols. DNA sequencing was performed on an ABI PRISM 3130XL or ABI PRISM 3730 Genetic Analyzer (Applied Biosystems) using the POP-7 polymer and ABI PRISM Genetic Analyzer Data Collection and ABI PRISM Genetic Analyzer Sequencing Analysis software. Shotgun reads were processed using Phred base calling software [138], [139] and monitored constantly against quality metrics using the Phred Q20. The quality scores for each run were monitored through a proprietary Galaxy LIMS system (Agencourt). Any substantial deviation from the normal range was investigated immediately.
Contig assembly
Shotgun reads were assembled using the Paracel GenomeAssembler (Paracel, Pasadena, CA) with default program parameters and quality scores. To assist in the final assembly and to monitor read quality, Paracel-derived contig sequences and their associated reads were entered into SeqMan Pro (v7.0, DNAstar, Madison, WI). Within each contig, all reads were then trimmed for quality at the 5′ and 3′ ends. Those reads that did not meet minimum quality requirements were discarded. A large number of sequence and contig gaps were present after assembly of the initial shotgun reads. Arrangement of these contigs into a final single contig used both standard PCR and combinatorial long and standard PCR to bridge the contig gaps; due to low sequence similarity and minimal synteny, existing epsilonproteobacterial genome sequences could not be used as a scaffold for the A. butzleri genome. All gaps, both sequence and contig, present in the assembly were closed by PCR using primers designed with Primer Premier (v 5.0, Premier Biosoft, Palo Alto, CA). The A. butzleri strain RM4018 genome contains five nearly identical ribosomal RNA (rRNA) loci of ∼6 kb. Each locus was amplified independently using unique flanking primer sets and long PCR. rRNA region amplicons were sequenced, using rRNA primers common to all five loci, and assembled. The assembled rRNA loci were then integrated into the larger genome assembly manually. Other large repeat regions were analyzed similarly.
The final assembly contained 27,377 reads: 12,990 small insert reads (average insert size ≅ 4200 bp), 7,364 large insert reads (average insert size ≅31,650 bp) and 7,023 PCR-based reads. The final assembly contained also contiguous sequences (>2× coverage/nt) on both strands for an average coverage of 5.5×; ambiguous bases were re-sequenced at least twice. Validation of the final assembly was achieved by comparison of mock in silico digestion patterns with recently published PFGE patterns using the type strain of A. butzleri [140]. Although the PFGE band sizes were on average ∼10% larger than those predicted in silico, there was an almost 100% correlation between the two banding patterns.
Genome analysis
Open reading frames were predicted using ORFscan (Rational Genomics, South San Francisco, CA) with the parameters set to include all three potential start codons (ATG, GTG and TTG) and a coding sequence (CDS) cut-off of 70 amino acids. Spurious CDSs (e.g. CDSs contained within larger CDSs) were deleted manually. Initial annotation of the putative CDSs was accomplished by comparing the predicted proteins to the nr database using BLASTP. The list of putative CDSs was then used to create a preliminary GenBank-formatted (.gbk) file that was entered into Artemis [141], [142]. Annotation within Artemis included the fusion of split CDSs into pseudogenes and the identification of small (usually ribosomal protein) genes overlooked in the initial ORFscan analysis. The start codon of each putative CDS was curated manually, either through visual inspection within Artemis of the ribosome binding site, or through BLAST comparison of each CDS to its epsilonproteobacterial homologs, where present; CDSs with dubious ribosome binding sites, lengths shorter than 200 bp and a low BLASTP score (E>10−10) were removed from the final list of CDSs. tRNAs were annotated using tRNAscan-SE [143], [144]. 16S and 23S rRNA loci were identified through BLASTN comparison to Campylobacter 16S and 23S loci; 5S rRNA loci were identified through BLASTN comparison to Helicobacter 5S loci.
Final annotation was performed using 1) BLASTP comparison to proteins in the NCBI nr database, 2) CDS identification, COG function prediction, cellular location prediction, and identification of Pfam domains and PROSITE motifs using the BASys Bacterial Annotation System [145] and 3) additional Pfam domain identification using the Sanger Centre Pfam search engine [146]. Four levels of gene assignment were used for each CDS: 1) CDSs predicted to encode proteins with high similarity across multiple taxa to proteins of known function (e.g. TrpF, RpsL, PurE) were assigned that function, 2) CDSs predicted to encode proteins with only a general function, but similar to members of various Pfam families or superfamilies, or containing various PROSITE motifs, were annotated to reflect the tentative general function (e.g. topoisomerase, ABC transporter, glycosyl transferase), 3) CDSs predicted to encode proteins with no defined function or motifs but similar to other non-defined proteins (E<10−10) across multiple bacterial taxa were designated as ‘conserved hypothetical’, and 4) CDSs predicted to encode proteins with no defined function and either unique to this genome or with a low similarity (E>10−10) to proteins from other taxa were designated as ‘hypothetical’. The complete nucleotide sequence and annotation of the A. butzleri strain RM4018 has been deposited at GenBank under the accession number CP000361.
Construction of the A. butzleri DNA microarray
DNA fragments of individual ORFs within A. butzleri strain RM4018 were amplified using primers designed with ArrayDesigner 3.0 (Premier Biosoft, Palo Alto, CA) and purchased from MWG Biotech Inc. (High Point, NC). Each PCR reaction (total reaction volume, 100 µl) consisted of 1× MasterAmp Taq PCR buffer, 1× MasterAmp Taq Enhancer, 2.5 mM MgCl2, 200 µM each dNTP, forward and reverse primers at 0.2 µM each, 0.5 U of MasterAmp Taq DNA polymerase (Epicentre), and approximately 50 ng of genomic DNA from A. butzleri strain RM4018. Thermal cycling was performed using a Tetrad thermal cycler (Bio-Rad, Hercules, CA) with the following amplification parameters: 30 cycles of 25 s at 94°C, 25 s at 52°C, and 2 min at 72°C and a final extension at 72°C for 5 min. PCR products were analyzed by gel electrophoresis in a 1% (w/v) agarose gel in 1× Tris-borate-EDTA buffer and stained with ethidium bromide. The DNA bands were examined under UV illumination. We amplified successfully a total of 2238 PCR products from A. butzleri strain RM4018. These PCR products were purified on a Qiagen 8000 robot using a Qiaquick 96-well Biorobot kit (Qiagen, Valencia, CA), dried, and resuspended to an average concentration of 0.1–0.2 µg µl−1 in 20 µl of 50% dimethyl sulfoxide (DMSO) containing 0.3× Saline-Sodium Citrate (SSC). All of the PCR probes were then spotted in duplicate on UltraGAPS slides (Corning) using an OmniGrid Accent (GeneMachines, Ann Arbor, MI) producing a final array that contained a total of 4476 features. The DNA was UV cross-linked to the microarray slides with a Stratalinker at 300 mJ (Stratagene, La Jolla, CA).
Preparation and fluorescent labeling of genomic DNA
Genomic DNA from A. butzleri was isolated as described previously [92]. For each microarray hybridization reaction, genomic DNA from the A. butzleri reference strain RM4018 and a test strain of A. butzleri were labeled fluorescently with indodicarbocyanine (Cy5)-dUTP and indocarbocyanine (Cy3)-dUTP, respectively. An aliquot (2 µg) of DNA was mixed with 5 µl 10× NEBlot labeling buffer containing random sequence octamer oligonucleotides (NEB, Beverly, MA.) and water to a final volume of 41 µl. This mixture was heated to 95°C for 5 min and then cooled for 5 min at 4°C. After this time, the remainder of the labeling reaction components were added: 5 µl of 10× dNTP labeling mix (1.2 mM each dATP, dGTP, dCTP; 0.5mM dTTP in 10 mM Tris pH 8.0; 1 mM EDTA), 3 µl of Cy3 dUTP or Cy5 dUTP (GE Biosciences, Piscataway, NJ) and 1 µl of Klenow fragment. The labeling reactions were incubated overnight at 37°C. Labeled DNA was purified from unincorporated label using Qiaquick PCR Cleanup kits. Labeling efficiencies were determined using a NanoDrop ND-1000 (NanoDrop, Wilmington, DE) and efficiently-labeled samples were dried by vacuum.
Microarray hybridization
Labeled reference and test DNAs were combined in 45 µl Pronto! cDNA hybridization solution (Corning, Corning, NY) and heated to 95°C for 5 min. Then, 15 µl of the hybridization mixture was put onto a microarray slide and sealed with a cover slip. The microarray slide was placed in a hybridization chamber (Corning) and incubated at 42°C for 18 h. Following hybridization, the slides were washed twice in 2× SSC, 0.1% sodium dodecyl sulfate at 42°C for 10 min, followed by twice in 1× SSC at room temperature for 10 min, and finally twice in 0.2× SSC at room temperature for 5 min. The microarray slides were dried by centrifugation at 300×g for 10 min before scanning. At least two hybridization reactions were performed for each test strain.
Microarray data analysis
Microarrays were scanned using an Axon GenePix 4000B microarray laser scanner (Axon Instruments, Inc. Union City, CA) as described previously [93]. Features (spots) and the local background intensities were detected and quantified with GenePix 4.0 software (Axon Instruments, Inc.). The data were filtered so that spots with a reference signal lower than the background plus 2 standard deviations of the background were discarded. Signal intensities were corrected by subtracting the local background, and then the Cy5/Cy3 ratios were calculated. The method for comparative genomic indexing (CGI) analysis utilized the GENCOM software described previously [132], [133] to assign present and absent CDS of A. butzleri strains when compared to reference strain RM4018. The Cy5 and Cy3 signal intensities were corrected by subtracting the local background before submission into the GENCOM program. GENCOM analysis was performed for each hybridization and the present/absent assignments for each CDS as determined by GENCOM were converted into scores (present = 1; absent = 0). If the CDS was assigned absent for the reference strain, this indicates that the test strain hybridized better than the reference. Considering all the CDS are from the reference strains, this may indicate that the CDS is in multicopy in the test strain. The CDS assigned absent for the reference strain, were given a score of 2 to indicate that the CDS may be in multicopy. The trinary CDS scores for all strains were analyzed further with GeneSpring microarray analysis software version 7.3 (Agilent Technologies, Redwood City, CA) and subjected to average-linkage hierarchical clustering with the standard correlation and bootstrapping. Microarray data have been deposited in the NCBI GEO repository (http://www.ncbi.nlm.nih.gov/geo/) with the accession number GSE8725.
Supporting Information
[Figure omitted. See PDF.]
Figure S1.
Diagram of the A. butzleri strain RM4018 genome. Genes and features are drawn to scale. Genes are colored according to role category. tRNA and rRNA loci are included. Prophage and genetic islands are shaded in grey.
https://doi.org/10.1371/journal.pone.0001358.s001
(0.17 MB PDF)
Figure S2.
Selected A. butzleri RM4018 signal transduction proteins. Diagrammatic representation of the Non-Che chemotaxis proteins and GGDEF domain diguanylate cyclases/EAL domain c-di-GMP phosphodiesterases predicted to be encoded by strain RM4018. Relevant motifs and transmembrane helices are indicated and drawn to scale.
https://doi.org/10.1371/journal.pone.0001358.s002
(0.03 MB PDF)
Table S1.
Arcobacter butzleri predicted open reading frames and their functional annotation. Complete list of A. butzleri strain RM4018 genes, tRNA loci and rRNA loci. Start and end coordinates are provided for each feature along with its functional annotation.
https://doi.org/10.1371/journal.pone.0001358.s003
(1.43 MB PDF)
Table S2.
Functional prediction of Arcobacter butzleri genes: similarity to proteins from other epsilonproteobacteria. A. butzleri strain RM4018 genes are sorted by functional category. The % amino acid identity of each protein to homologs in other epsilonproteobacteria is listed.
https://doi.org/10.1371/journal.pone.0001358.s004
(3.33 MB PDF)
Table S3.
Similarity of Arcobacter butzleri proteins to proteins from other taxa. The five best homologs (where applicable) for each protein in strain RM4018 are listed, as well as the Expect (E) value, % identity and % similarity.
https://doi.org/10.1371/journal.pone.0001358.s005
(4.71 MB PDF)
Table S4.
Antibiotic resistance profiles of strain RM4018 and selected Campylobacters. Comparison of the antibiotic resistance profiles of A. butzleri strain RM4018, C. jejuni strain NCTC 11168, C. coli strain RM2228, C. lari strain RM2100 and C. upsaliensis strain RM3195.
https://doi.org/10.1371/journal.pone.0001358.s006
(0.02 MB PDF)
Table S5.
Trinary CGI data as determined with GENCOM. Trinary A. butzleri DNA microarray data for the reference strain RM4018 and the 12 additional A. butzleri tester strains.
https://doi.org/10.1371/journal.pone.0001358.s007
(0.91 MB XLS)
Table S6.
CDS contained in all A. butzleri strains examined. Core set of 1676 genes identified in RM4018 and all 12 additional A. butzleri strains by comparative genomic indexing. The functional annotation of each gene is provided.
https://doi.org/10.1371/journal.pone.0001358.s008
(0.51 MB PDF)
Table S7.
CDS present only in A. butzleri strain RM4018. Set of 42 genes identified by comparative genomic indexing of 13 A. butzleri strains to be present only in strain RM4018. The functional annotation of each gene is provided.
https://doi.org/10.1371/journal.pone.0001358.s009
(0.02 MB PDF)
Acknowledgments
We thank Keith Fagerquist for critical reading of this manuscript.
Author Contributions
Conceived and designed the experiments: WM CP. Performed the experiments: WM CP MR GW JM AR. Analyzed the data: WM CP DU PH JS MR GM MW TB JM AR. Contributed reagents/materials/analysis tools: WM CP MR JM AR. Wrote the paper: WM CP JS GM MW. Other: Initiated the project, Final assembly and annotation, Comparative genomic analyses: WM. Construction of DNA microarray and DNA microarray analysis: CP. Shotgun sequencing and assembly: MR JM AR. Designed Figure 1 for the manuscript: DU TB PH. Gap closure sequencing: GW. Secured funds to support the study: LS RM.
Citation: Miller WG, Parker CT, Rubenfield M, Mendz GL, Wösten MMSM, Ussery DW, et al. (2007) The Complete Genome Sequence and Analysis of the Epsilonproteobacterium Arcobacter butzleri. PLoS ONE2(12): e1358. https://doi.org/10.1371/journal.pone.0001358
1. Skirrow MB, Blaser MJ (2000) Clinical aspects of Campylobacter infection. In: Nachamkin I, Blaser MJ, editors. Campylobacter. 2nd ed. ed. Washington, DC: ASM Press. pp. 69–88.MB SkirrowMJ Blaser2000Clinical aspects of Campylobacter infection.I. NachamkinMJ BlaserCampylobacter. 2nd ed. edWashington, DCASM Press6988
2. Marshall B (2001) One hundred years of discovery and rediscovery of Helicobacter pylori and its association with peptic ulcer disease. In: Mobley HL, Mendz GL, Hazell SL, editors. Helicobacter pylori: Physiology and genetics. Washington, DC: ASM Press. pp. 19–24.B. Marshall2001One hundred years of discovery and rediscovery of Helicobacter pylori and its association with peptic ulcer disease.HL MobleyGL MendzSL HazellHelicobacter pylori: Physiology and geneticsWashington, DCASM Press1924
3. Sandstedt K, Ursing J, Walder M (1983) Thermotolerant Campylobacter with no or weak catalase activity isolated from dogs. Curr Microbiol 8: 209–213.K. SandstedtJ. UrsingM. Walder1983Thermotolerant Campylobacter with no or weak catalase activity isolated from dogs.Curr Microbiol8209213
4. Fox JG, Cabot EB, Taylor NS, Laraway R (1988) Gastric colonization by Campylobacter pylori subsp. mustelae in ferrets. Infect Immun 56: 2994–2996.JG FoxEB CabotNS TaylorR. Laraway1988Gastric colonization by Campylobacter pylori subsp. mustelae in ferrets.Infect Immun5629942996
5. Vandamme P (2000) Taxonomy of the Family Campylobacteraceae. In: Nachamkin I, Blaser MJ, editors. Campylobacter. Washington, DC: ASM Press. pp. 3–26.P. Vandamme2000Taxonomy of the Family Campylobacteraceae.I. NachamkinMJ BlaserCampylobacterWashington, DCASM Press326
6. Kiehlbauch JA, Brenner DJ, Nicholson MA, Baker CN, Patton CM, et al. (1991) Campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness. J Clin Microbiol 29: 376–385.JA KiehlbauchDJ BrennerMA NicholsonCN BakerCM Patton1991Campylobacter butzleri sp. nov. isolated from humans and animals with diarrheal illness.J Clin Microbiol29376385
7. Neill SD, Campbell JN, O'Brien JJ, Weatherup STC, Ellis WA (1985) Taxonomic position of Campylobacter cryaerophila sp. nov. Int J Syst Bacteriol 35: 342–356.SD NeillJN CampbellJJ O'BrienSTC WeatherupWA Ellis1985Taxonomic position of Campylobacter cryaerophila sp. nov.Int J Syst Bacteriol35342356
8. Vandamme P, Vancanneyt M, Pot B, Mels L, Hoste B, et al. (1992) Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42: 344–356.P. VandammeM. VancanneytB. PotL. MelsB. Hoste1992Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens.Int J Syst Bacteriol42344356
9. McClung CR, Patriquin DG, Davis RE (1983) Campylobacter nitrofigilis sp nov., a nitrogen fixing bacterium associated with roots of Spartina aterniflora Loisel. Int J Syst Bacteriol 33: 605–612.CR McClungDG PatriquinRE Davis1983Campylobacter nitrofigilis sp nov., a nitrogen fixing bacterium associated with roots of Spartina aterniflora Loisel.Int J Syst Bacteriol33605612
10. Forsythe SJ (2006) Arcobacter. In: Motarjemi Y, Adams M, editors. Emerging foodborne pathogens. Cambridge, England: Woodhead Publishing Ltd. pp. 181–221.SJ Forsythe2006Arcobacter.Y. MotarjemiM. AdamsEmerging foodborne pathogensCambridge, EnglandWoodhead Publishing Ltd181221
11. Donachie SP, Bowman JP, On SL, Alam M (2005) Arcobacter halophilus sp. nov., the first obligate halophile in the genus Arcobacter. Int J Syst Evol Microbiol 55: 1271–1277.SP DonachieJP BowmanSL OnM. Alam2005Arcobacter halophilus sp. nov., the first obligate halophile in the genus Arcobacter.Int J Syst Evol Microbiol5512711277
12. Houf K, On SL, Coenye T, Mast J, Van Hoof J, et al. (2005) Arcobacter cibarius sp. nov., isolated from broiler carcasses. Int J Syst Evol Microbiol 55: 713–717.K. HoufSL OnT. CoenyeJ. MastJ. Van Hoof2005Arcobacter cibarius sp. nov., isolated from broiler carcasses.Int J Syst Evol Microbiol55713717
13. Wirsen CO, Sievert SM, Cavanaugh CM, Molyneaux SJ, Ahmad A, et al. (2002) Characterization of an autotrophic sulfide-oxidizing marine Arcobacter sp. that produces filamentous sulfur. Appl Environ Microbiol 68: 316–325.CO WirsenSM SievertCM CavanaughSJ MolyneauxA. Ahmad2002Characterization of an autotrophic sulfide-oxidizing marine Arcobacter sp. that produces filamentous sulfur.Appl Environ Microbiol68316325
14. Moussard H, Corre E, Cambon-Bonavita MA, Fouquet Y, Jeanthon C (2006) Novel uncultured Epsilonproteobacteria dominate a filamentous sulphur mat from the 13 degrees N hydrothermal vent field, East Pacific Rise. H. MoussardE. CorreMA Cambon-BonavitaY. FouquetC. Jeanthon2006Novel uncultured Epsilonproteobacteria dominate a filamentous sulphur mat from the 13 degrees N hydrothermal vent field, East Pacific Rise
15. Prabagaran SR, Manorama R, Delille D, Shivaji S (2006) Predominance of Roseobacter, Sulfitobacter, Glaciecola and Psychrobacter in seawater collected off Ushuaia, Argentina, Sub-Antarctica. FEMS Microbiol Ecol. SR PrabagaranR. ManoramaD. DelilleS. Shivaji2006Predominance of Roseobacter, Sulfitobacter, Glaciecola and Psychrobacter in seawater collected off Ushuaia, Argentina, Sub-Antarctica.FEMS Microbiol Ecol
16. Grabowski A, Nercessian O, Fayolle F, Blanchet D, Jeanthon C (2005) Microbial diversity in production waters of a low-temperature biodegraded oil reservoir. FEMS Microbiol Ecol 54: 427–443.A. GrabowskiO. NercessianF. FayolleD. BlanchetC. Jeanthon2005Microbial diversity in production waters of a low-temperature biodegraded oil reservoir.FEMS Microbiol Ecol54427443
17. Frias-Lopez J, Zerkle AL, Bonheyo GT, Fouke BW (2002) Partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces. Appl Environ Microbiol 68: 2214–2228.J. Frias-LopezAL ZerkleGT BonheyoBW Fouke2002Partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces.Appl Environ Microbiol6822142228
18. Thamdrup B, Rossello-Mora R, Amann R (2000) Microbial manganese and sulfate reduction in Black Sea shelf sediments. Appl Environ Microbiol 66: 2888–2897.B. ThamdrupR. Rossello-MoraR. Amann2000Microbial manganese and sulfate reduction in Black Sea shelf sediments.Appl Environ Microbiol6628882897
19. Naganuma T, Kato C, Hirayama H, Moriyama N, Hashimoto J, et al. (1996) Intracellular occurence of epsiloproteobacterial 16S rDNA sequences in the vestimentiferan trophosome. J Oceanography 53: 193–197.T. NaganumaC. KatoH. HirayamaN. MoriyamaJ. Hashimoto1996Intracellular occurence of epsiloproteobacterial 16S rDNA sequences in the vestimentiferan trophosome.J Oceanography53193197
20. Snaidr J, Amann R, Huber I, Ludwig W, Schleifer KH (1997) Phylogenetic analysis and in situ identification of bacteria in activated sludge. Appl Environ Microbiol 63: 2884–2896.J. SnaidrR. AmannI. HuberW. LudwigKH Schleifer1997Phylogenetic analysis and in situ identification of bacteria in activated sludge.Appl Environ Microbiol6328842896
21. McGarvey JA, Miller WG, Sanchez S, Silva CJ, Whitehand LC (2005) Comparison of bacterial populations and chemical composition of dairy wastewater held in circulated and stagnant lagoons. J Appl Microbiol 99: 867–877.JA McGarveyWG MillerS. SanchezCJ SilvaLC Whitehand2005Comparison of bacterial populations and chemical composition of dairy wastewater held in circulated and stagnant lagoons.J Appl Microbiol99867877
22. Assanta MA, Roy D, Lemay MJ, Montpetit D (2002) Attachment of Arcobacter butzleri, a new waterborne pathogen, to water distribution pipe surfaces. J Food Prot 65: 1240–1247.MA AssantaD. RoyMJ LemayD. Montpetit2002Attachment of Arcobacter butzleri, a new waterborne pathogen, to water distribution pipe surfaces.J Food Prot6512401247
23. Dhamabutra N, Kamol-Rathanakul P, Pienthaweechai K (1992) Isolation of Campylobacters from the canals of Bangkok metropolitan area. J Med Assoc Thai 75: 350–364.N. DhamabutraP. Kamol-RathanakulK. Pienthaweechai1992Isolation of Campylobacters from the canals of Bangkok metropolitan area.J Med Assoc Thai75350364
24. Fera MT, Maugeri TL, Gugliandolo C, Beninati C, Giannone M, et al. (2004) Detection of Arcobacter spp. in the coastal environment of the Mediterranean Sea. Appl Environ Microbiol 70: 1271–1276.MT FeraTL MaugeriC. GugliandoloC. BeninatiM. Giannone2004Detection of Arcobacter spp. in the coastal environment of the Mediterranean Sea.Appl Environ Microbiol7012711276
25. Jacob J, Lior H, Feuerpfeil I (1993) Isolation of Arcobacter butzleri from a drinking water reservoir in eastern Germany. Zentralbl Hyg Umweltmed 193: 557–562.J. JacobH. LiorI. Feuerpfeil1993Isolation of Arcobacter butzleri from a drinking water reservoir in eastern Germany.Zentralbl Hyg Umweltmed193557562
26. Jacob J, Woodward D, Feuerpfeil I, Johnson WM (1998) Isolation of Arcobacter butzleri in raw water and drinking water treatment plants in Germany. Zentralbl Hyg Umweltmed 201: 189–198.J. JacobD. WoodwardI. FeuerpfeilWM Johnson1998Isolation of Arcobacter butzleri in raw water and drinking water treatment plants in Germany.Zentralbl Hyg Umweltmed201189198
27. Maugeri TL, Gugliandolo C, Carbone M, Caccamo D, Fera MT (2000) Isolation of Arcobacter spp. from a brackish environment. New Microbiol 23: 143–149.TL MaugeriC. GugliandoloM. CarboneD. CaccamoMT Fera2000Isolation of Arcobacter spp. from a brackish environment.New Microbiol23143149
28. Maugeri TL, Irrera GP, Lentini V, Carbone M, Fera MT, et al. (2005) Detection and enumeration of Arcobacter spp. in the coastal environment of the Straits of Messina (Italy). New Microbiol 28: 177–182.TL MaugeriGP IrreraV. LentiniM. CarboneMT Fera2005Detection and enumeration of Arcobacter spp. in the coastal environment of the Straits of Messina (Italy).New Microbiol28177182
29. Rice EW, Rodgers MR, Wesley IV, Johnson CH, Tanner SA (1999) Isolation of Arcobacter butzleri from ground water. Lett Appl Microbiol 28: 31–35.EW RiceMR RodgersIV WesleyCH JohnsonSA Tanner1999Isolation of Arcobacter butzleri from ground water.Lett Appl Microbiol283135
30. Stampi S, De Luca G, Varoli O, Zanetti F (1999) Occurrence, removal and seasonal variation of thermophilic campylobacters and Arcobacter in sewage sludge. Zentralbl Hyg Umweltmed 202: 19–27.S. StampiG. De LucaO. VaroliF. Zanetti1999Occurrence, removal and seasonal variation of thermophilic campylobacters and Arcobacter in sewage sludge.Zentralbl Hyg Umweltmed2021927
31. Mandrell RE, Miller WG (2006) Campylobacter. In: Motarjemi Y, Adams M, editors. Emerging foodborne pathogens. Cambridge, England: Woodhead Publishing Ltd. pp. 476–521.RE MandrellWG Miller2006Campylobacter.Y. MotarjemiM. AdamsEmerging foodborne pathogensCambridge, EnglandWoodhead Publishing Ltd476521
32. Hume ME, Harvey RB, Stanker LH, Droleskey RE, Poole TL, et al. (2001) Genotypic variation among arcobacter isolates from a farrow-to-finish swine facility. J Food Prot 64: 645–651.ME HumeRB HarveyLH StankerRE DroleskeyTL Poole2001Genotypic variation among arcobacter isolates from a farrow-to-finish swine facility.J Food Prot64645651
33. Collins CI, Wesley IV, Murano EA (1996) Detection of Arcobacter spp. in ground pork by modified plating methods. J Food Protect 59: 448–452.CI CollinsIV WesleyEA Murano1996Detection of Arcobacter spp. in ground pork by modified plating methods.J Food Protect59448452
34. Rivas L, Fegan N, Vanderlinde P (2004) Isolation and characterisation of Arcobacter butzleri from meat. Int J Food Microbiol 91: 31–41.L. RivasN. FeganP. Vanderlinde2004Isolation and characterisation of Arcobacter butzleri from meat.Int J Food Microbiol913141
35. Atabay HI, Aydin F, Houf K, Sahin M, Vandamme P (2003) The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE. Int J Food Microbiol 81: 21–28.HI AtabayF. AydinK. HoufM. SahinP. Vandamme2003The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey, and identification of the isolates using SDS-PAGE.Int J Food Microbiol812128
36. Houf K, Devriese LA, De Zutter L, Van Hoof J, Vandamme P (2001) Development of a new protocol for the isolation and quantification of Arcobacter species from poultry products. Int J Food Microbiol 71: 189–196.K. HoufLA DevrieseL. De ZutterJ. Van HoofP. Vandamme2001Development of a new protocol for the isolation and quantification of Arcobacter species from poultry products.Int J Food Microbiol71189196
37. Manke TR, Wesley IV, Dickson JS, Harmon KM (1998) Prevalence and genetic variability of Arcobacter species in mechanically separated turkey. J Food Prot 61: 1623–1628.TR MankeIV WesleyJS DicksonKM Harmon1998Prevalence and genetic variability of Arcobacter species in mechanically separated turkey.J Food Prot6116231628
38. Golla SC, Murano EA, Johnson LG, Tipton NC, Cureington EA, et al. (2002) Determination of the occurrence of Arcobacter butzleri in beef and dairy cattle from Texas by various isolation methods. J Food Prot 65: 1849–1853.SC GollaEA MuranoLG JohnsonNC TiptonEA Cureington2002Determination of the occurrence of Arcobacter butzleri in beef and dairy cattle from Texas by various isolation methods.J Food Prot6518491853
39. Wesley IV, Wells SJ, Harmon KM, Green A, Schroeder-Tucker L, et al. (2000) Fecal shedding of Campylobacter and Arcobacter spp. in dairy cattle. Appl Environ Microbiol 66: 1994–2000.IV WesleySJ WellsKM HarmonA. GreenL. Schroeder-Tucker2000Fecal shedding of Campylobacter and Arcobacter spp. in dairy cattle.Appl Environ Microbiol6619942000
40. van Driessche E, Houf K, van Hoof J, De Zutter L, Vandamme P (2003) Isolation of Arcobacter species from animal feces. FEMS Microbiol Lett 229: 243–248.E. van DriesscheK. HoufJ. van HoofL. De ZutterP. Vandamme2003Isolation of Arcobacter species from animal feces.FEMS Microbiol Lett229243248
41. Engberg J, On SL, Harrington CS, Gerner-Smidt P (2000) Prevalence of Campylobacter, Arcobacter, Helicobacter, and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for Campylobacters. J Clin Microbiol 38: 286–291.J. EngbergSL OnCS HarringtonP. Gerner-Smidt2000Prevalence of Campylobacter, Arcobacter, Helicobacter, and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for Campylobacters.J Clin Microbiol38286291
42. Lastovica AJ, Skirrow MB (2000) Clinical significance of Campylobacter and related species other than Campylobacter jejuni and C. coli. In: Nachamkin I, Blaser MJ, editors. Campylobacter. Washington, DC: ASM Press. pp. 89–120.AJ LastovicaMB Skirrow2000Clinical significance of Campylobacter and related species other than Campylobacter jejuni and C. coli.I. NachamkinMJ BlaserCampylobacterWashington, DCASM Press89120
43. Lerner J, Brumberger V, Preac-Mursic V (1994) Severe diarrhea associated with Arcobacter butzleri. Eur J Clin Microbiol Infect Dis 13: 660–662.J. LernerV. BrumbergerV. Preac-Mursic1994Severe diarrhea associated with Arcobacter butzleri.Eur J Clin Microbiol Infect Dis13660662
44. Taylor DN, Kiehlbauch JA, Tee W, Pitarangsi C, Echeverria P (1991) Isolation of group 2 aerotolerant Campylobacter species from Thai children with diarrhea. J Infect Dis 163: 1062–1067.DN TaylorJA KiehlbauchW. TeeC. PitarangsiP. Echeverria1991Isolation of group 2 aerotolerant Campylobacter species from Thai children with diarrhea.J Infect Dis16310621067
45. Vandamme P, Pugina P, Benzi G, Van Etterijck R, Vlaes L, et al. (1992) Outbreak of recurrent abdominal cramps associated with Arcobacter butzleri in an Italian school. J Clin Microbiol 30: 2335–2337.P. VandammeP. PuginaG. BenziR. Van EtterijckL. Vlaes1992Outbreak of recurrent abdominal cramps associated with Arcobacter butzleri in an Italian school.J Clin Microbiol3023352337
46. Prouzet-Mauleon V, Labadi L, Bouges N, Menard A, Megraud F (2006) Arcobacter butzleri: underestimated enteropathogen. Emerg Infect Dis 12: 307–309.V. Prouzet-MauleonL. LabadiN. BougesA. MenardF. Megraud2006Arcobacter butzleri: underestimated enteropathogen.Emerg Infect Dis12307309
47. Vandenberg O, Dediste A, Houf K, Ibekwem S, Souayah H, et al. (2004) Arcobacter species in humans. Emerg Infect Dis 10: 1863–1867.O. VandenbergA. DedisteK. HoufS. IbekwemH. Souayah2004Arcobacter species in humans.Emerg Infect Dis1018631867
48. Lau SK, Woo PC, Teng JL, Leung KW, Yuen KY (2002) Identification by 16S ribosomal RNA gene sequencing of Arcobacter butzleri bacteraemia in a patient with acute gangrenous appendicitis. Mol Pathol 55: 182–185.SK LauPC WooJL TengKW LeungKY Yuen2002Identification by 16S ribosomal RNA gene sequencing of Arcobacter butzleri bacteraemia in a patient with acute gangrenous appendicitis.Mol Pathol55182185
49. Yan JJ, Ko WC, Huang AH, Chen HM, Jin YT, et al. (2000) Arcobacter butzleri bacteremia in a patient with liver cirrhosis. J Formos Med Assoc 99: 166–169.JJ YanWC KoAH HuangHM ChenYT Jin2000Arcobacter butzleri bacteremia in a patient with liver cirrhosis.J Formos Med Assoc99166169
50. Houf K, Stephan R (2007) Isolation and characterization of the emerging foodborn pathogen Arcobacter from human stool. J Microbiol Methods 68: 408–413.K. HoufR. Stephan2007Isolation and characterization of the emerging foodborn pathogen Arcobacter from human stool.J Microbiol Methods68408413
51. Parkhill J, Wren BW, Mungall K, Ketley JM, Churcher C, et al. (2000) The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403: 665–668.J. ParkhillBW WrenK. MungallJM KetleyC. Churcher2000The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences.Nature403665668
52. Fouts DE, Mongodin EF, Mandrell RE, Miller WG, Rasko DA, et al. (2005) Major structural differences and novel potential virulence mechanisms from the genomes of multiple campylobacter species. PLoS Biol 3: e15.DE FoutsEF MongodinRE MandrellWG MillerDA Rasko2005Major structural differences and novel potential virulence mechanisms from the genomes of multiple campylobacter species.PLoS Biol3e15
53. Hofreuter D, Tsai J, Watson RO, Novik V, Altman B, et al. (2006) Unique features of a highly pathogenic Campylobacter jejuni strain. Infect Immun 74: 4694–4707.D. HofreuterJ. TsaiRO WatsonV. NovikB. Altman2006Unique features of a highly pathogenic Campylobacter jejuni strain.Infect Immun7446944707
54. Pearson BM, Gaskin DJ, Segers RP, Wells JM, Nuijten PJ, et al. (2007) The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828). J Bacteriol 189: 8402–8403.BM PearsonDJ GaskinRP SegersJM WellsPJ Nuijten2007The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828).J Bacteriol18984028403
55. Takai K, Suzuki M, Nakagawa S, Miyazaki M, Suzuki Y, et al. (2006) Sulfurimonas paralvinellae sp. nov., a novel mesophilic, hydrogen- and sulfur-oxidizing chemolithoautotroph within the Epsilonproteobacteria isolated from a deep-sea hydrothermal vent polychaete nest, reclassification of Thiomicrospira denitrificans as Sulfurimonas denitrificans comb. nov. and emended description of the genus Sulfurimonas. Int J Syst Evol Microbiol 56: 1725–1733.K. TakaiM. SuzukiS. NakagawaM. MiyazakiY. Suzuki2006Sulfurimonas paralvinellae sp. nov., a novel mesophilic, hydrogen- and sulfur-oxidizing chemolithoautotroph within the Epsilonproteobacteria isolated from a deep-sea hydrothermal vent polychaete nest, reclassification of Thiomicrospira denitrificans as Sulfurimonas denitrificans comb. nov. and emended description of the genus Sulfurimonas.Int J Syst Evol Microbiol5617251733
56. Baar C, Eppinger M, Raddatz G, Simon J, Lanz C, et al. (2003) Complete genome sequence and analysis of Wolinella succinogenes. Proc Natl Acad Sci U S A 100: 11690–11695.C. BaarM. EppingerG. RaddatzJ. SimonC. Lanz2003Complete genome sequence and analysis of Wolinella succinogenes.Proc Natl Acad Sci U S A1001169011695
57. Suerbaum S, Josenhans C, Sterzenbach T, Drescher B, Brandt P, et al. (2003) The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus. Proc Natl Acad Sci U S A 100: 7901–7906.S. SuerbaumC. JosenhansT. SterzenbachB. DrescherP. Brandt2003The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus.Proc Natl Acad Sci U S A10079017906
58. Tomb JF, White O, Kerlavage AR, Clayton RA, Sutton GG, et al. (1997) The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388: 539–547.JF TombO. WhiteAR KerlavageRA ClaytonGG Sutton1997The complete genome sequence of the gastric pathogen Helicobacter pylori.Nature388539547
59. Alm RA, Ling LS, Moir DT, King BL, Brown ED, et al. (1999) Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397: 176–180.RA AlmLS LingDT MoirBL KingED Brown1999Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori.Nature397176180
60. Oh JD, Kling-Backhed H, Giannakis M, Xu J, Fulton RS, et al. (2006) The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: evolution during disease progression. Proc Natl Acad Sci U S A 103: 9999–10004.JD OhH. Kling-BackhedM. GiannakisJ. XuRS Fulton2006The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: evolution during disease progression.Proc Natl Acad Sci U S A103999910004
61. Eppinger M, Baar C, Linz B, Raddatz G, Lanz C, et al. (2006) Who ate whom? Adaptive Helicobacter genomic changes that accompanied a host jump from early humans to large felines. PLoS Genet 2: e120.M. EppingerC. BaarB. LinzG. RaddatzC. Lanz2006Who ate whom? Adaptive Helicobacter genomic changes that accompanied a host jump from early humans to large felines.PLoS Genet2e120
62. Nakagawa S, Takaki Y, Shimamura S, Reysenbach AL, Takai K, et al. (2007) Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens. Proc Natl Acad Sci U S A 104: 12146–12150.S. NakagawaY. TakakiS. ShimamuraAL ReysenbachK. Takai2007Deep-sea vent epsilon-proteobacterial genomes provide insights into emergence of pathogens.Proc Natl Acad Sci U S A1041214612150
63. Iyer RR, Pluciennik A, Burdett V, Modrich PL (2006) DNA mismatch repair: functions and mechanisms. Chem Rev 106: 302–323.RR IyerA. PluciennikV. BurdettPL Modrich2006DNA mismatch repair: functions and mechanisms.Chem Rev106302323
64. Malik HS, Henikoff S (2000) Dual recognition-incision enzymes might be involved in mismatch repair and meiosis. Trends Biochem Sci 25: 414–418.HS MalikS. Henikoff2000Dual recognition-incision enzymes might be involved in mismatch repair and meiosis.Trends Biochem Sci25414418
65. Viswanathan M, Lovett ST (1998) Single-strand DNA-specific exonucleases in Escherichia coli. Roles in repair and mutation avoidance. Genetics 149: 7–16.M. ViswanathanST Lovett1998Single-strand DNA-specific exonucleases in Escherichia coli. Roles in repair and mutation avoidance.Genetics149716
66. Viswanathan M, Lovett ST (1999) Exonuclease X of Escherichia coli. A novel 3′-5′ DNase and DnaQ superfamily member involved in DNA repair. J Biol Chem 274: 30094–30100.M. ViswanathanST Lovett1999Exonuclease X of Escherichia coli. A novel 3′-5′ DNase and DnaQ superfamily member involved in DNA repair.J Biol Chem2743009430100
67. Kredich NM (1996) Biosynthesis of cysteine. In: Neidhardt FC, editor. Escherichia coli and Salmonella: Cellular and molecular biology. 2nd ed. ed. Washington DC: ASM Press. pp. 514–527.NM Kredich1996Biosynthesis of cysteine.FC NeidhardtEscherichia coli and Salmonella: Cellular and molecular biology. 2nd ed. edWashington DCASM Press514527
68. Neuwald AF, Krishnan BR, Brikun I, Kulakauskas S, Suziedelis K, et al. (1992) cysQ, a gene needed for cysteine synthesis in Escherichia coli K-12 only during aerobic growth. J Bacteriol 174: 415–425.AF NeuwaldBR KrishnanI. BrikunS. KulakauskasK. Suziedelis1992cysQ, a gene needed for cysteine synthesis in Escherichia coli K-12 only during aerobic growth.J Bacteriol174415425
69. Valdes J, Veloso F, Jedlicki E, Holmes D (2003) Metabolic reconstruction of sulfur assimilation in the extremophile Acidithiobacillus ferrooxidans based on genome analysis. BMC Genomics 4: 51.J. ValdesF. VelosoE. JedlickiD. Holmes2003Metabolic reconstruction of sulfur assimilation in the extremophile Acidithiobacillus ferrooxidans based on genome analysis.BMC Genomics451
70. Bick JA, Dennis JJ, Zylstra GJ, Nowack J, Leustek T (2000) Identification of a new class of 5′-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria. J Bacteriol 182: 135–142.JA BickJJ DennisGJ ZylstraJ. NowackT. Leustek2000Identification of a new class of 5′-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria.J Bacteriol182135142
71. Williams SJ, Senaratne RH, Mougous JD, Riley LW, Bertozzi CR (2002) 5′-adenosinephosphosulfate lies at a metabolic branch point in mycobacteria. J Biol Chem 277: 32606–32615.SJ WilliamsRH SenaratneJD MougousLW RileyCR Bertozzi20025′-adenosinephosphosulfate lies at a metabolic branch point in mycobacteria.J Biol Chem2773260632615
72. Friedrich CG, Quentmeier A, Bardischewsky F, Rother D, Kraft R, et al. (2000) Novel genes coding for lithotrophic sulfur oxidation of Paracoccus pantotrophus GB17. J Bacteriol 182: 4677–4687.CG FriedrichA. QuentmeierF. BardischewskyD. RotherR. Kraft2000Novel genes coding for lithotrophic sulfur oxidation of Paracoccus pantotrophus GB17.J Bacteriol18246774687
73. Wodara C, Bardischewsky F, Friedrich CG (1997) Cloning and characterization of sulfite dehydrogenase, two c-type cytochromes, and a flavoprotein of Paracoccus denitrificans GB17: essential role of sulfite dehydrogenase in lithotrophic sulfur oxidation. J Bacteriol 179: 5014–5023.C. WodaraF. BardischewskyCG Friedrich1997Cloning and characterization of sulfite dehydrogenase, two c-type cytochromes, and a flavoprotein of Paracoccus denitrificans GB17: essential role of sulfite dehydrogenase in lithotrophic sulfur oxidation.J Bacteriol17950145023
74. Eisen JA, Nelson KE, Paulsen IT, Heidelberg JF, Wu M, et al. (2002) The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium. Proc Natl Acad Sci U S A 99: 9509–9514.JA EisenKE NelsonIT PaulsenJF HeidelbergM. Wu2002The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium.Proc Natl Acad Sci U S A9995099514
75. Appia-Ayme C, Little PJ, Matsumoto Y, Leech AP, Berks BC (2001) Cytochrome complex essential for photosynthetic oxidation of both thiosulfate and sulfide in Rhodovulum sulfidophilum. J Bacteriol 183: 6107–6118.C. Appia-AymePJ LittleY. MatsumotoAP LeechBC Berks2001Cytochrome complex essential for photosynthetic oxidation of both thiosulfate and sulfide in Rhodovulum sulfidophilum.J Bacteriol18361076118
76. Friedrich CG, Rother D, Bardischewsky F, Quentmeier A, Fischer J (2001) Oxidation of reduced inorganic sulfur compounds by bacteria: emergence of a common mechanism? Appl Environ Microbiol 67: 2873–2882.CG FriedrichD. RotherF. BardischewskyA. QuentmeierJ. Fischer2001Oxidation of reduced inorganic sulfur compounds by bacteria: emergence of a common mechanism?Appl Environ Microbiol6728732882
77. Appia-Ayme C, Berks BC (2002) SoxV, an orthologue of the CcdA disulfide transporter, is involved in thiosulfate oxidation in Rhodovulum sulfidophilum and reduces the periplasmic thioredoxin SoxW. Biochem Biophys Res Commun 296: 737–741.C. Appia-AymeBC Berks2002SoxV, an orthologue of the CcdA disulfide transporter, is involved in thiosulfate oxidation in Rhodovulum sulfidophilum and reduces the periplasmic thioredoxin SoxW.Biochem Biophys Res Commun296737741
78. Bagchi A, Ghosh TC (2006) Structural insight into the interactions of SoxV, SoxW and SoxS in the process of transport of reductants during sulfur oxidation by the novel global sulfur oxidation reaction cycle. Biophys Chem 119: 7–13.A. BagchiTC Ghosh2006Structural insight into the interactions of SoxV, SoxW and SoxS in the process of transport of reductants during sulfur oxidation by the novel global sulfur oxidation reaction cycle.Biophys Chem119713
79. Grimek TL, Escalante-Semerena JC (2004) The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo. J Bacteriol 186: 454–462.TL GrimekJC Escalante-Semerena2004The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo.J Bacteriol186454462
80. Friedmann S, Steindorf A, Alber BE, Fuchs G (2006) Properties of succinyl-coenzyme A:L-malate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus. J Bacteriol 188: 2646–2655.S. FriedmannA. SteindorfBE AlberG. Fuchs2006Properties of succinyl-coenzyme A:L-malate coenzyme A transferase and its role in the autotrophic 3-hydroxypropionate cycle of Chloroflexus aurantiacus.J Bacteriol18826462655
81. Hallam SJ, Mincer TJ, Schleper C, Preston CM, Roberts K, et al. (2006) Pathways of carbon assimilation and ammonia oxidation suggested by environmental genomic analyses of marine Crenarchaeota. PLoS Biol 4: e95.SJ HallamTJ MincerC. SchleperCM PrestonK. Roberts2006Pathways of carbon assimilation and ammonia oxidation suggested by environmental genomic analyses of marine Crenarchaeota.PLoS Biol4e95
82. Berg IA, Filatova LV, Ivanovsky RN (2002) Inhibition of acetate and propionate assimilation by itaconate via propionyl-CoA carboxylase in isocitrate lyase-negative purple bacterium Rhodospirillum rubrum. FEMS Microbiol Lett 216: 49–54.IA BergLV FilatovaRN Ivanovsky2002Inhibition of acetate and propionate assimilation by itaconate via propionyl-CoA carboxylase in isocitrate lyase-negative purple bacterium Rhodospirillum rubrum.FEMS Microbiol Lett2164954
83. Ivanovsky RN, Krasilnikova EN, Berg IA (1997) A proposed citramalate cycle for acetate assimilation in the purple non-sulfur bacterium Rhodospirillum rubrum. FEMS Microbiol Lett 153: 399–404.RN IvanovskyEN KrasilnikovaIA Berg1997A proposed citramalate cycle for acetate assimilation in the purple non-sulfur bacterium Rhodospirillum rubrum.FEMS Microbiol Lett153399404
84. Takagi K, Torimura M, Kawaguchi K, Kano K, Ikeda T (1999) Biochemical and electrochemical characterization of quinohemoprotein amine dehydrogenase from Paracoccus denitrificans. Biochemistry 38: 6935–6942.K. TakagiM. TorimuraK. KawaguchiK. KanoT. Ikeda1999Biochemical and electrochemical characterization of quinohemoprotein amine dehydrogenase from Paracoccus denitrificans.Biochemistry3869356942
85. Vandenberghe I, Kim JK, Devreese B, Hacisalihoglu A, Iwabuki H, et al. (2001) The covalent structure of the small subunit from Pseudomonas putida amine dehydrogenase reveals the presence of three novel types of internal cross-linkages, all involving cysteine in a thioether bond. J Biol Chem 276: 42923–42931.I. VandenbergheJK KimB. DevreeseA. HacisalihogluH. Iwabuki2001The covalent structure of the small subunit from Pseudomonas putida amine dehydrogenase reveals the presence of three novel types of internal cross-linkages, all involving cysteine in a thioether bond.J Biol Chem2764292342931
86. Takagi K, Yamamoto K, Kano K, Ikeda T (2001) New pathway of amine oxidation respiratory chain of Paracoccus denitrificans IFO 12442. Eur J Biochem 268: 470–476.K. TakagiK. YamamotoK. KanoT. Ikeda2001New pathway of amine oxidation respiratory chain of Paracoccus denitrificans IFO 12442.Eur J Biochem268470476
87. Adachi O, Yoshihara N, Tanasupawat S, Toyama H, Matsushita K (2003) Purification and characterization of membrane-bound quinoprotein quinate dehydrogenase. Biosci Biotechnol Biochem 67: 2115–2123.O. AdachiN. YoshiharaS. TanasupawatH. ToyamaK. Matsushita2003Purification and characterization of membrane-bound quinoprotein quinate dehydrogenase.Biosci Biotechnol Biochem6721152123
88. Ono K, Okajima T, Tani M, Kuroda S, Sun D, et al. (2006) Involvement of a putative [Fe-S]-cluster-binding protein in the biogenesis of quinohemoprotein amine dehydrogenase. J Biol Chem 281: 13672–13684.K. OnoT. OkajimaM. TaniS. KurodaD. Sun2006Involvement of a putative [Fe-S]-cluster-binding protein in the biogenesis of quinohemoprotein amine dehydrogenase.J Biol Chem2811367213684
89. Hacisalihoglu A, Jongejan JA, Duine JA (1997) Distribution of amine oxidases and amine dehydrogenases in bacteria grown on primary amines and characterization of the amine oxidase from Klebsiella oxytoca. Microbiology 143 ( Pt 2): 505–512.A. HacisalihogluJA JongejanJA Duine1997Distribution of amine oxidases and amine dehydrogenases in bacteria grown on primary amines and characterization of the amine oxidase from Klebsiella oxytoca.Microbiology143 ( Pt 2)505512
90. Goldie J, Veldhuyzen van Zanten SJ, Jalali S, Hollingsworth J, Riddell RH, et al. (1989) Optimization of a medium for the rapid urease test for detection of Campylobacter pylori in gastric antral biopsies. J Clin Microbiol 27: 2080–2082.J. GoldieSJ Veldhuyzen van ZantenS. JalaliJ. HollingsworthRH Riddell1989Optimization of a medium for the rapid urease test for detection of Campylobacter pylori in gastric antral biopsies.J Clin Microbiol2720802082
91. Gilbert M, Godschalk P, Parker CT, Endtz H, Wakarchuk W (2005) Genetic basis for the variation in the lipooligosaccharide outer core of Campylobacter jejuni and possible association of glycosyltransferase genes with post-infectious neuropathies. In: Ketley JM, Konkel ME, editors. Campylobacter: Molecular and Cellular Biology. Norfolk, UK: Horizon Bioscience. pp. 219–248.M. GilbertP. GodschalkCT ParkerH. EndtzW. Wakarchuk2005Genetic basis for the variation in the lipooligosaccharide outer core of Campylobacter jejuni and possible association of glycosyltransferase genes with post-infectious neuropathies.JM KetleyME KonkelCampylobacter: Molecular and Cellular BiologyNorfolk, UKHorizon Bioscience219248
92. Parker CT, Horn ST, Gilbert M, Miller WG, Woodward DL, et al. (2005) Comparison of Campylobacter jejuni lipooligosaccharide biosynthesis loci from a variety of sources. J Clin Microbiol 43: 2771–2781.CT ParkerST HornM. GilbertWG MillerDL Woodward2005Comparison of Campylobacter jejuni lipooligosaccharide biosynthesis loci from a variety of sources.J Clin Microbiol4327712781
93. Parker CT, Quinones B, Miller WG, Horn ST, Mandrell RE (2006) Comparative genomic analysis of Campylobacter jejuni strains reveals diversity due to genomic elements similar to those present in C. jejuni strain RM1221. J Clin Microbiol 44: 4125–4135.CT ParkerB. QuinonesWG MillerST HornRE Mandrell2006Comparative genomic analysis of Campylobacter jejuni strains reveals diversity due to genomic elements similar to those present in C. jejuni strain RM1221.J Clin Microbiol4441254135
94. Kaniuk NA, Monteiro MA, Parker CT, Whitfield C (2002) Molecular diversity of the genetic loci responsible for lipopolysaccharide core oligosaccharide assembly within the genus Salmonella. Mol Microbiol 46: 1305–1318.NA KaniukMA MonteiroCT ParkerC. Whitfield2002Molecular diversity of the genetic loci responsible for lipopolysaccharide core oligosaccharide assembly within the genus Salmonella.Mol Microbiol4613051318
95. Burrows LL, Charter DF, Lam JS (1996) Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster. Mol Microbiol 22: 481–495.LL BurrowsDF CharterJS Lam1996Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.Mol Microbiol22481495
96. Morgan GJ, Hatfull GF, Casjens S, Hendrix RW (2002) Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus. J Mol Biol 317: 337–359.GJ MorganGF HatfullS. CasjensRW Hendrix2002Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus.J Mol Biol317337359
97. Summer EJ, Gonzalez CF, Carlisle T, Mebane LM, Cass AM, et al. (2004) Burkholderia cenocepacia phage BcepMu and a family of Mu-like phages encoding potential pathogenesis factors. J Mol Biol 340: 49–65.EJ SummerCF GonzalezT. CarlisleLM MebaneAM Cass2004Burkholderia cenocepacia phage BcepMu and a family of Mu-like phages encoding potential pathogenesis factors.J Mol Biol3404965
98. Atabay HI, Aydin F (2001) Susceptibility of Arcobacter butzleri isolates to 23 antimicrobial agents. Lett Appl Microbiol 33: 430–433.HI AtabayF. Aydin2001Susceptibility of Arcobacter butzleri isolates to 23 antimicrobial agents.Lett Appl Microbiol33430433
99. Bayles KW (2000) The bactericidal action of penicillin: new clues to an unsolved mystery. Trends Microbiol 8: 274–278.KW Bayles2000The bactericidal action of penicillin: new clues to an unsolved mystery.Trends Microbiol8274278
100. Groicher KH, Firek BA, Fujimoto DF, Bayles KW (2000) The Staphylococcus aureus lrgAB operon modulates murein hydrolase activity and penicillin tolerance. J Bacteriol 182: 1794–1801.KH GroicherBA FirekDF FujimotoKW Bayles2000The Staphylococcus aureus lrgAB operon modulates murein hydrolase activity and penicillin tolerance.J Bacteriol18217941801
101. Glaab WE, Mitchell LS, Miller JE, Vlasakova K, Skopek TR (2005) 5-fluorouracil forward mutation assay in Salmonella: determination of mutational target and spontaneous mutational spectra. Mutat Res 578: 238–246.WE GlaabLS MitchellJE MillerK. VlasakovaTR Skopek20055-fluorouracil forward mutation assay in Salmonella: determination of mutational target and spontaneous mutational spectra.Mutat Res578238246
102. Martinussen J, Hammer K (1994) Cloning and characterization of upp, a gene encoding uracil phosphoribosyltransferase from Lactococcus lactis. J Bacteriol 176: 6457–6463.J. MartinussenK. Hammer1994Cloning and characterization of upp, a gene encoding uracil phosphoribosyltransferase from Lactococcus lactis.J Bacteriol17664576463
103. Taylor DE, Tracz DM (2005) Mechanisms of antimicrobial resistance in Campylobacter. In: Ketley JM, Konkel ME, editors. Campylobacter: Molecular and Cellular biology. Norfolk, UK: Horizon Bioscience. pp. 193–204.DE TaylorDM Tracz2005Mechanisms of antimicrobial resistance in Campylobacter.JM KetleyME KonkelCampylobacter: Molecular and Cellular biologyNorfolk, UKHorizon Bioscience193204
104. Abdelbaqi K, Menard A, Prouzet-Mauleon V, Bringaud F, Lehours P, et al. (2007) Nucleotide sequence of the gyrA gene of Arcobacter species and characterization of human ciprofloxacin-resistant clinical isolates. FEMS Immunol Med Microbiol 49: 337–345.K. AbdelbaqiA. MenardV. Prouzet-MauleonF. BringaudP. Lehours2007Nucleotide sequence of the gyrA gene of Arcobacter species and characterization of human ciprofloxacin-resistant clinical isolates.FEMS Immunol Med Microbiol49337345
105. Ruiz J (2003) Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection. J Antimicrob Chemother 51: 1109–1117.J. Ruiz2003Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection.J Antimicrob Chemother5111091117
106. Yoshida H, Bogaki M, Nakamura S, Ubukata K, Konno M (1990) Nucleotide sequence and characterization of the Staphylococcus aureus norA gene, which confers resistance to quinolones. J Bacteriol 172: 6942–6949.H. YoshidaM. BogakiS. NakamuraK. UbukataM. Konno1990Nucleotide sequence and characterization of the Staphylococcus aureus norA gene, which confers resistance to quinolones.J Bacteriol17269426949
107. Pickett CL, Lee RB (2005) Cytolethal distending toxin. In: Ketley JM, Konkel ME, editors. Campylobacter: Molecular and Cellular biology. Norfolk, UK: Horizon Bioscience. pp. 385–396.CL PickettRB Lee2005Cytolethal distending toxin.JM KetleyME KonkelCampylobacter: Molecular and Cellular biologyNorfolk, UKHorizon Bioscience385396
108. Johnson LG, Murano EA (2002) Lack of a cytolethal distending toxin among Arcobacter isolates from various sources. J Food Prot 65: 1789–1795.LG JohnsonEA Murano2002Lack of a cytolethal distending toxin among Arcobacter isolates from various sources.J Food Prot6517891795
109. Jin S, Joe A, Lynett J, Hani EK, Sherman P, et al. (2001) JlpA, a novel surface-exposed lipoprotein specific to Campylobacter jejuni, mediates adherence to host epithelial cells. Mol Microbiol 39: 1225–1236.S. JinA. JoeJ. LynettEK HaniP. Sherman2001JlpA, a novel surface-exposed lipoprotein specific to Campylobacter jejuni, mediates adherence to host epithelial cells.Mol Microbiol3912251236
110. Pei Z, Blaser MJ (1993) PEB1, the major cell-binding factor of Campylobacter jejuni, is a homolog of the binding component in gram-negative nutrient transport systems. J Biol Chem 268: 18717–18725.Z. PeiMJ Blaser1993PEB1, the major cell-binding factor of Campylobacter jejuni, is a homolog of the binding component in gram-negative nutrient transport systems.J Biol Chem2681871718725
111. Goldberg MB, DiRita VJ, Calderwood SB (1990) Identification of an iron-regulated virulence determinant in Vibrio cholerae, using TnphoA mutagenesis. Infect Immun 58: 55–60.MB GoldbergVJ DiRitaSB Calderwood1990Identification of an iron-regulated virulence determinant in Vibrio cholerae, using TnphoA mutagenesis.Infect Immun585560
112. Johnson JR, Jelacic S, Schoening LM, Clabots C, Shaikh N, et al. (2005) The IrgA homologue adhesin Iha is an Escherichia coli virulence factor in murine urinary tract infection. Infect Immun 73: 965–971.JR JohnsonS. JelacicLM SchoeningC. ClabotsN. Shaikh2005The IrgA homologue adhesin Iha is an Escherichia coli virulence factor in murine urinary tract infection.Infect Immun73965971
113. Larsen NA, Lin H, Wei R, Fischbach MA, Walsh CT (2006) Structural characterization of enterobactin hydrolase IroE. Biochemistry 45: 10184–10190.NA LarsenH. LinR. WeiMA FischbachCT Walsh2006Structural characterization of enterobactin hydrolase IroE.Biochemistry451018410190
114. Rojas CM, Ham JH, Deng WL, Doyle JJ, Collmer A (2002) HecA, a member of a class of adhesins produced by diverse pathogenic bacteria, contributes to the attachment, aggregation, epidermal cell killing, and virulence phenotypes of Erwinia chrysanthemi EC16 on Nicotiana clevelandii seedlings. Proc Natl Acad Sci U S A 99: 13142–13147.CM RojasJH HamWL DengJJ DoyleA. Collmer2002HecA, a member of a class of adhesins produced by diverse pathogenic bacteria, contributes to the attachment, aggregation, epidermal cell killing, and virulence phenotypes of Erwinia chrysanthemi EC16 on Nicotiana clevelandii seedlings.Proc Natl Acad Sci U S A991314213147
115. Korolik V, Ketley JM (2006) Campylobacter chemotaxis. In: Ketley JM, Konkel ME, editors. Campylobacter: Molecular and Cellular Biology. Norfolk, UK: Horizon Bioscience. pp. 349–367.V. KorolikJM Ketley2006Campylobacter chemotaxis.JM KetleyME KonkelCampylobacter: Molecular and Cellular BiologyNorfolk, UKHorizon Bioscience349367
116. Szurmant H, Ordal GW (2004) Diversity in chemotaxis mechanisms among the bacteria and archaea. Microbiol Mol Biol Rev 68: 301–319.H. SzurmantGW Ordal2004Diversity in chemotaxis mechanisms among the bacteria and archaea.Microbiol Mol Biol Rev68301319
117. Tsang N, Macnab R, Koshland DE Jr (1973) Common mechanism for repellents and attractants in bacterial chemotaxis. Science 181: 60–63.N. TsangR. MacnabDE Koshland Jr1973Common mechanism for repellents and attractants in bacterial chemotaxis.Science1816063
118. Anantharaman V, Aravind L (2000) Cache - a signaling domain common to animal Ca(2+)-channel subunits and a class of prokaryotic chemotaxis receptors. Trends Biochem Sci 25: 535–537.V. AnantharamanL. Aravind2000Cache - a signaling domain common to animal Ca(2+)-channel subunits and a class of prokaryotic chemotaxis receptors.Trends Biochem Sci25535537
119. Niehus E, Gressmann H, Ye F, Schlapbach R, Dehio M, et al. (2004) Genome-wide analysis of transcriptional hierarchy and feedback regulation in the flagellar system of Helicobacter pylori. Mol Microbiol 52: 947–961.E. NiehusH. GressmannF. YeR. SchlapbachM. Dehio2004Genome-wide analysis of transcriptional hierarchy and feedback regulation in the flagellar system of Helicobacter pylori.Mol Microbiol52947961
120. Wosten MM, Wagenaar JA, van Putten JP (2004) The FlgS/FlgR two-component signal transduction system regulates the fla regulon in Campylobacter jejuni. J Biol Chem 279: 16214–16222.MM WostenJA WagenaarJP van Putten2004The FlgS/FlgR two-component signal transduction system regulates the fla regulon in Campylobacter jejuni.J Biol Chem2791621416222
121. Chilcott GS, Hughes KT (2000) Coupling of flagellar gene expression to flagellar assembly in Salmonella enterica serovar typhimurium and Escherichia coli. Microbiol Mol Biol Rev 64: 694–708.GS ChilcottKT Hughes2000Coupling of flagellar gene expression to flagellar assembly in Salmonella enterica serovar typhimurium and Escherichia coli.Microbiol Mol Biol Rev64694708
122. Sourjik V, Muschler P, Scharf B, Schmitt R (2000) VisN and VisR are global regulators of chemotaxis, flagellar, and motility genes in Sinorhizobium (Rhizobium) meliloti. J Bacteriol 182: 782–788.V. SourjikP. MuschlerB. ScharfR. Schmitt2000VisN and VisR are global regulators of chemotaxis, flagellar, and motility genes in Sinorhizobium (Rhizobium) meliloti.J Bacteriol182782788
123. Hughes KT, Mathee K (1998) The anti-sigma factors. Annu Rev Microbiol 52: 231–286.KT HughesK. Mathee1998The anti-sigma factors.Annu Rev Microbiol52231286
124. Kazmierczak MJ, Wiedmann M, Boor KJ (2005) Alternative sigma factors and their roles in bacterial virulence. Microbiol Mol Biol Rev 69: 527–543.MJ KazmierczakM. WiedmannKJ Boor2005Alternative sigma factors and their roles in bacterial virulence.Microbiol Mol Biol Rev69527543
125. Romling U, Amikam D (2006) Cyclic di-GMP as a second messenger. Curr Opin Microbiol 9: 218–228.U. RomlingD. Amikam2006Cyclic di-GMP as a second messenger.Curr Opin Microbiol9218228
126. Romling U, Gomelsky M, Galperin MY (2005) C-di-GMP: the dawning of a novel bacterial signalling system. Mol Microbiol 57: 629–639.U. RomlingM. GomelskyMY Galperin2005C-di-GMP: the dawning of a novel bacterial signalling system.Mol Microbiol57629639
127. Gjermansen M, Ragas P, Tolker-Nielsen T (2006) Proteins with GGDEF and EAL domains regulate Pseudomonas putida biofilm formation and dispersal. FEMS Microbiol Lett. M. GjermansenP. RagasT. Tolker-Nielsen2006Proteins with GGDEF and EAL domains regulate Pseudomonas putida biofilm formation and dispersal.FEMS Microbiol Lett
128. Da Re S, Ghigo JM (2006) A CsgD-independent pathway for cellulose production and biofilm formation in Escherichia coli. J Bacteriol 188: 3073–3087.S. Da ReJM Ghigo2006A CsgD-independent pathway for cellulose production and biofilm formation in Escherichia coli.J Bacteriol18830733087
129. Kazmierczak BI, Lebron MB, Murray TS (2006) Analysis of FimX, a phosphodiesterase that governs twitching motility in Pseudomonas aeruginosa. Mol Microbiol 60: 1026–1043.BI KazmierczakMB LebronTS Murray2006Analysis of FimX, a phosphodiesterase that governs twitching motility in Pseudomonas aeruginosa.Mol Microbiol6010261043
130. O'Shea T M, Klein AH, Geszvain K, Wolfe AJ, Visick KL (2006) Diguanylate cyclases control magnesium-dependent motility of Vibrio fischeri. J Bacteriol. M. O'Shea TAH KleinK. GeszvainAJ WolfeKL Visick2006Diguanylate cyclases control magnesium-dependent motility of Vibrio fischeri.J Bacteriol
131. Tischler AD, Camilli A (2005) Cyclic diguanylate regulates Vibrio cholerae virulence gene expression. Infect Immun 73: 5873–5882.AD TischlerA. Camilli2005Cyclic diguanylate regulates Vibrio cholerae virulence gene expression.Infect Immun7358735882
132. Pearson BM, Pin C, Wright J, I'Anson K, Humphrey T, et al. (2003) Comparative genome analysis of Campylobacter jejuni using whole genome DNA microarrays. FEBS Lett 554: 224–230.BM PearsonC. PinJ. WrightK. I'AnsonT. Humphrey2003Comparative genome analysis of Campylobacter jejuni using whole genome DNA microarrays.FEBS Lett554224230
133. Pin C, Reuter M, Pearson B, Friis L, Overweg K, et al. (2006) Comparison of different approaches for comparative genetic analysis using microarray hybridization. Appl Microbiol Biotechnol 72: 852–859.C. PinM. ReuterB. PearsonL. FriisK. Overweg2006Comparison of different approaches for comparative genetic analysis using microarray hybridization.Appl Microbiol Biotechnol72852859
134. Houf K, Tutenel A, De Zutter L, Van Hoof J, Vandamme P (2000) Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. FEMS Microbiol Lett 193: 89–94.K. HoufA. TutenelL. De ZutterJ. Van HoofP. Vandamme2000Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii.FEMS Microbiol Lett1938994
135. Miller WG, Pearson BM, Wells JM, Parker CT, Kapitonov VV, et al. (2005) Diversity within the Campylobacter jejuni type I restriction-modification loci. Microbiology 151: 337–351.WG MillerBM PearsonJM WellsCT ParkerVV Kapitonov2005Diversity within the Campylobacter jejuni type I restriction-modification loci.Microbiology151337351
136. DeAngelis MM, Wang DG, Hawkins TL (1995) Solid-phase reversible immobilization for the isolation of PCR products. Nucleic Acids Res 23: 4742–4743.MM DeAngelisDG WangTL Hawkins1995Solid-phase reversible immobilization for the isolation of PCR products.Nucleic Acids Res2347424743
137. Hawkins TL, McKernan KJ, Jacotot LB, MacKenzie JB, Richardson PM, et al. (1997) A magnetic attraction to high-throughput genomics. Science 276: 1887–1889.TL HawkinsKJ McKernanLB JacototJB MacKenziePM Richardson1997A magnetic attraction to high-throughput genomics.Science27618871889
138. Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res 8: 186–194.B. EwingP. Green1998Base-calling of automated sequencer traces using phred. II. Error probabilities.Genome Res8186194
139. Ewing B, Hillier L, Wendl MC, Green P (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res 8: 175–185.B. EwingL. HillierMC WendlP. Green1998Base-calling of automated sequencer traces using phred. I. Accuracy assessment.Genome Res8175185
140. Stoeva K, Bruce Ward F (2006) Genome mapping of Arcobacter butzleri. FEMS Microbiol Lett 256: 290–297.K. StoevaF. Bruce Ward2006Genome mapping of Arcobacter butzleri.FEMS Microbiol Lett256290297
141. Artemis (release 8.0). Artemis (release 8.0).[http://www.sanger.ac.uk/Software/Artemis/]. [http://www.sanger.ac.uk/Software/Artemis/].
142. Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, et al. (2000) Artemis: sequence visualization and annotation. Bioinformatics 16: 944–945.K. RutherfordJ. ParkhillJ. CrookT. HorsnellP. Rice2000Artemis: sequence visualization and annotation.Bioinformatics16944945
143. tRNAscan-SE (v. 1.23). tRNAscan-SE (v. 1.23).[http://bioweb.pasteur.fr/seqanal/interfaces/trnascan.html]. [http://bioweb.pasteur.fr/seqanal/interfaces/trnascan.html].
144. Lowe TM, Eddy SR (1997) tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 25: 955–964.TM LoweSR Eddy1997tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.Nucleic Acids Res25955964
145. BASys Bacterial Annotation System. BASys Bacterial Annotation System.[http://wishart.biology.ualberta.ca/basys/cgi/submit.pl]. [http://wishart.biology.ualberta.ca/basys/cgi/submit.pl].
146. Sanger Centre Pfam search engine. Sanger Centre Pfam search engine.[http://www.sanger.ac.uk/Software/Pfam/]. [http://www.sanger.ac.uk/Software/Pfam/].
147. Zoomable Arcobacter butzleri strain RM4018 BLAST atlas. Zoomable Arcobacter butzleri strain RM4018 BLAST atlas.[http://www.cbs.dtu.dk/services/GenomeAtlas/suppl/zoomatlas/zpidArcobacter_butzleri]. [http://www.cbs.dtu.dk/services/GenomeAtlas/suppl/zoomatlas/zpidArcobacter_butzleri].
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Abstract
Background
Arcobacter butzleri is a member of the epsilon subdivision of the Proteobacteria and a close taxonomic relative of established pathogens, such as Campylobacter jejuni and Helicobacter pylori. Here we present the complete genome sequence of the human clinical isolate, A. butzleri strain RM4018.
Methodology/Principal Findings
Arcobacter butzleri is a member of the Campylobacteraceae, but the majority of its proteome is most similar to those of Sulfuromonas denitrificans and Wolinella succinogenes, both members of the Helicobacteraceae, and those of the deep-sea vent Epsilonproteobacteria Sulfurovum and Nitratiruptor. In addition, many of the genes and pathways described here, e.g. those involved in signal transduction and sulfur metabolism, have been identified previously within the epsilon subdivision only in S. denitrificans, W. succinogenes, Sulfurovum, and/or Nitratiruptor, or are unique to the subdivision. In addition, the analyses indicated also that a substantial proportion of the A. butzleri genome is devoted to growth and survival under diverse environmental conditions, with a large number of respiration-associated proteins, signal transduction and chemotaxis proteins and proteins involved in DNA repair and adaptation. To investigate the genomic diversity of A. butzleri strains, we constructed an A. butzleri DNA microarray comprising 2238 genes from strain RM4018. Comparative genomic indexing analysis of 12 additional A. butzleri strains identified both the core genes of A. butzleri and intraspecies hypervariable regions, where <70% of the genes were present in at least two strains.
Conclusion/Significance
The presence of pathways and loci associated often with non-host-associated organisms, as well as genes associated with virulence, suggests that A. butzleri is a free-living, water-borne organism that might be classified rightfully as an emerging pathogen. The genome sequence and analyses presented in this study are an important first step in understanding the physiology and genetics of this organism, which constitutes a bridge between the environment and mammalian hosts.
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