Abstract

Due to their capacity to skew T cell responses towards Th1 oriented immunity, oligonucleotides containing unmethylated CpG motifs (CpG) have emerged as interesting adjuvants for vaccination. Whereas the signalling pathways in response to CpG mediated TLR9 activation have been extensively documented at the level of the individual cell, little is however known on the precise identity of the innate immune cells that govern T cell priming and polarisation to CpG adjuvanted protein antigens in vivo. In this study, we demonstrate that optimal induction of Th1 oriented immunity to CpG adjuvanted protein vaccines requires the coordinated actions of conventional DCs and of monocytes. Whilst conventional DCs were required for antigen presentation and initial T cell priming, monocytes constitute the main source of the Th1 polarising cytokine IL-12.

Details

Title
Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12
Author
De Koker, S 1 ; L Van Hoecke 2 ; De Beuckelaer, A 3 ; Roose, K 2 ; Deswarte, K 4 ; Willart, M A 4 ; Bogaert, P 3 ; Naessens, T 3 ; De Geest, B G 5 ; Saelens, X 2   VIAFID ORCID Logo  ; Lambrecht, B N 4 ; Grooten, J 3 

1  Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium; Department of Biochemistry, Ghent University, Ghent, Belgium; VIB Medical Biotechnology Center, Ghent University, Ghent, Belgium 
2  Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium; VIB Medical Biotechnology Center, Ghent University, Ghent, Belgium 
3  Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium 
4  VIB Inflammation Research Center, Ghent University, Zwijnaarde, Belgium; Department of Respiratory Medicine, University Hospital Ghent, Ghent, Belgium 
5  Department of Pharmaceutics, Ghent University, Ghent, Belgium 
Pages
1-14
Publication year
2017
Publication date
Jul 2017
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-017-06236-6
ProQuest document ID
1956172327
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.