Abstract

Human biliary tree stem/progenitor cells (hBTSCs) are being used for cell therapies of patients with liver cirrhosis. A cryopreservation method was established to optimize sourcing of hBTSCs for these clinical programs and that comprises serum-free Kubota’s Medium (KM) supplemented with 10% dimethyl sulfoxide (DMSO), 15% human serum albumin (HSA) and 0.1% hyaluronans. Cryopreserved versus freshly isolated hBTSCs were similar in vitro with respect to self-replication, stemness traits, and multipotency. They were able to differentiate to functional hepatocytes,cholangiocytes or pancreatic islets, yielding similar levels of secretion of albumin or of glucose-inducible levels of insulin. Cryopreserved versus freshly isolated hBTSCs were equally able to engraft into immunocompromised mice yielding cells with human-specific gene expression and human albumin levels in murine serum that were higher for cryopreserved than for freshly isolated hBTSCs. The successful cryopreservation of hBTSCs facilitates establishment of hBTSCs cell banking offering logistical advantages for clinical programs for treatment of liver diseases.

Details

Title
Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors
Author
Nevi, Lorenzo 1   VIAFID ORCID Logo  ; Cardinale, Vincenzo 1 ; Carpino, Guido 2 ; Costantini, Daniele 1 ; Sabina Di Matteo 1 ; Cantafora, Alfredo 1 ; Melandro, Fabio 3 ; Brunelli, Roberto 4 ; Bastianelli, Carlo 4   VIAFID ORCID Logo  ; Aliberti, Camilla 4 ; Monti, Marco 4 ; Bosco, Daniela 5 ; Pasquale Bartolomeo Berloco 3 ; Pierluigi Benedetti Panici 4 ; Reid, Lola 6 ; Gaudio, Eugenio 7 ; Alvaro, Domenico 8 

1  Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Rome, Italy 
2  Department of Movement, Human and Health Sciences, Division of Health Sciences, University of Rome “Foro Italico”, Rome, Italy 
3  Department of General Surgery and Organ Transplantation, Sapienza University of Rome, Rome, Italy 
4  Department of Gynaecologic-Obstetric and Urologic Sciences, Sapienza University of Rome, Rome, Italy 
5  Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy 
6  Department of Cell Biology and Physiology and Program in Molecular Biology and Biotechnology, University of North Carolina School of Medicine, North Carolina, USA 
7  Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome, Italy 
8  Department of Medicine and Medical Specialties, Sapienza University of Rome, Rome, Italy 
Pages
1-15
Publication year
2017
Publication date
Jul 2017
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-017-05858-0
ProQuest document ID
1956173158
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.