Abstract

Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.

Details

Title
Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish
Author
Lagendijk, Anne Karine 1   VIAFID ORCID Logo  ; Gomez, Guillermo A 2   VIAFID ORCID Logo  ; Baek, Sungmin 1   VIAFID ORCID Logo  ; Hesselson, Daniel 3   VIAFID ORCID Logo  ; Hughes, William E 3 ; Paterson, Scott 1 ; Conway, Daniel E 4   VIAFID ORCID Logo  ; Heinz-Georg Belting 5   VIAFID ORCID Logo  ; Affolter, Markus 5   VIAFID ORCID Logo  ; Smith, Kelly A 1   VIAFID ORCID Logo  ; Schwartz, Martin A 6   VIAFID ORCID Logo  ; Yap, Alpha S 7   VIAFID ORCID Logo  ; Hogan, Benjamin M 1   VIAFID ORCID Logo 

1  Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland, St Lucia, QLD, Australia 
2  Institute for Molecular Bioscience, Cell Biology and Molecular Medicine division, The University of Queensland, St Lucia, QLD, Australia; Centre for Cancer Biology, SA Pathology and the University of South Australia, Adelaide, SA, Australia 
3  Garvan Institute of Medical Research, Darlinghurst, Sydney, NSW, Australia 
4  Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA, USA 
5  Biozentrum der Universität Basel, Basel, Switzerland 
6  Yale Cardiovascular Research Center and Department of Internal Medicine, Cardiovascular Medicine, Yale University School of Medicine, New Haven, CT, USA 
7  Institute for Molecular Bioscience, Cell Biology and Molecular Medicine division, The University of Queensland, St Lucia, QLD, Australia 
Pages
1-12
Publication year
2017
Publication date
Nov 2017
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-017-01325-6
ProQuest document ID
1962258938
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.