Abstract

Routine laboratory lipid assays include simple measurements of total cholesterol, triacylglycerols and HDL. However, lipids are a large group of compounds involved in many metabolic pathways, and their alterations may have serious health consequences. In this study, we used 1H NMR to analyze lipids extracted from sera of 16 obese patients prior to and after bariatric surgeries. We observed a post-surgery decrease in serum concentrations of lipids from various groups. The hereby presented findings imply that 1H NMR is suitable for rapid, simple and non-invasive detection of lipids from 30 structural groups, among them triacylglycerols, phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, total phospholipids, total, free and esterified cholesterol, total and unsaturated fatty acids. NMR-based analysis of serum lipids may contribute to a substantial increase in the number of routinely determined markers from this group; therefore, it may find application in clinical assessment of obese subjects prior to and after bariatric surgeries, as well as in the examination of patients with other metabolic diseases.

Details

Title
Potential Application of 1H NMR for Routine Serum Lipidome Analysis –Evaluation of Effects of Bariatric Surgery
Author
Mika, Adriana 1   VIAFID ORCID Logo  ; Kaczynski, Zbigniew 2 ; Stepnowski, Piotr 3 ; Kaczor, Maciej 4 ; Proczko-Stepaniak, Monika 4 ; Kaska, Lukasz 4 ; Sledzinski, Tomasz 5 

1  Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, Gdansk, Poland; Department of Pharmaceutical Biochemistry, Medical University of Gdansk, Debinki 1, Gdansk, Poland 
2  Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, Gdansk, Poland 
3  Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, Gdansk, Poland 
4  Department of General, Endocrine and Transplant Surgery, Medical University of Gdansk, Smoluchowskiego 17, Gdansk, Poland 
5  Department of Pharmaceutical Biochemistry, Medical University of Gdansk, Debinki 1, Gdansk, Poland 
Pages
1-9
Publication year
2017
Publication date
Nov 2017
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-017-15346-0
ProQuest document ID
1964084599
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.