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The Figure 5 legend is:pVHL is phosphorylated on Ser72 by Aurora-A in vitroA- Purified recombinant 6xHis-tagged pVHL30 [VHL(His)6] (lane 1) or mutant 6xHis-tagged pVHL30 S72A [VHLSA72(His)6] ( (lane 2) were incubated in the presence of [γ-32P] ATP and recombinant Aurora-A(His)6. Samples were separated by SDS-PAGE and the gel was stained with Coomassie Blue (higher panel). pVHL phosphorylation status was analysed by autoradiography (lower panel).The asterix (*) indicate the pVHL protein. B- Localization and sequence of the putative Aurora-A phosphorylation site on pVHL.
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Citation: Martin B, Chesnel F, Delcros J-G, Jouan F, Couturier A, Dugay F, et al. (2014) Correction: Identification of pVHL as a Novel Substrate for Aurora-A in Clear Cell Renal Cell Carcinoma (ccRCC). PLoS ONE 9(1): 10.1371/annotation/d506055a-d16b-4c0c-aef6-ad39e91a3b8d. https://doi.org/10.1371/annotation/d506055a-d16b-4c0c-aef6-ad39e91a3b8d
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