Abstract

The distinct spatial architecture of the apical actin cables (or actin cap) facilitates rapid biophysical signaling between extracellular mechanical stimuli and intracellular responses, including nuclear shaping, cytoskeletal remodeling, and the mechanotransduction of external forces into biochemical signals. These functions are abrogated in lamin A/C-deficient mouse embryonic fibroblasts that recapitulate the defective nuclear organization of laminopathies, featuring disruption of the actin cap. However, how nuclear lamin A/C mediates the ability of the actin cap to regulate nuclear morphology remains unclear. Here, we show that lamin A/C expressing cells can form an actin cap to resist nuclear deformation in response to physiological mechanical stresses. This study reveals how the nuclear lamin A/C-mediated formation of the perinuclear apical actin cables protects the nuclear structural integrity from extracellular physical disturbances. Our findings highlight the role of the physical interactions between the cytoskeletal network and the nucleus in cellular mechanical homeostasis.

Details

Title
Nuclear lamin A/C harnesses the perinuclear apical actin cables to protect nuclear morphology
Author
Jeong-Ki, Kim 1 ; Louhghalam, Arghavan 2 ; Lee, Geonhui 1 ; Schafer, Benjamin W 3 ; Wirtz, Denis 4 ; Dong-Hwee, Kim 1   VIAFID ORCID Logo 

1  KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, South Korea 
2  Department of Civil and Environmental Engineering, University of Massachusetts Dartmouth, Dartmouth, MA, USA 
3  Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore, MD, USA; Department of Civil Engineering, The John Hopkins University, Baltimore, MD, USA 
4  Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore, MD, USA; Department of Chemical and Biomolecular Engineering, The John Hopkins University, Baltimore, MD, USA; Johns Hopkins Physical Sciences—Oncology Center, The Johns Hopkins University, Baltimore, MD, USA; Departments of Pathology and Oncology and Sydney Kimmel Comprehensive Cancer Center, The Johns Hopkins School of Medicine, Baltimore, MD, USA 
Pages
1-13
Publication year
2017
Publication date
Dec 2017
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-017-02217-5
ProQuest document ID
1983416016
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.