Abstract

The transcription co-activator complex SAGA is recruited to gene promoters by sequence-specific transcriptional activators and by chromatin modifications to promote pre-initiation complex formation. The yeast Tra1 subunit is the major target of acidic activators such as Gal4, VP16, or Gcn4 but little is known about its structural organization. The 430 kDa Tra1 subunit and its human homolog the transformation/transcription domain-associated protein TRRAP are members of the phosphatidyl 3-kinase-related kinase (PIKK) family. Here, we present the cryo-EM structure of the entire SAGA complex where the major target of activator binding, the 430 kDa Tra1 protein, is resolved with an average resolution of 5.7 Å. The high content of alpha-helices in Tra1 enabled tracing of the majority of its main chain. Our results highlight the integration of Tra1 within the major epigenetic regulator SAGA.

Details

Title
Structure of the transcription activator target Tra1 within the chromatin modifying complex SAGA
Author
Sharov, Grigory 1   VIAFID ORCID Logo  ; Voltz, Karine 1 ; Durand, Alexandre 2 ; Kolesnikova, Olga 1 ; Papai, Gabor 1 ; Myasnikov, Alexander G 3 ; Dejaegere, Annick 1 ; Adam Ben Shem 1 ; Schultz, Patrick 1   VIAFID ORCID Logo 

1  Department of Integrated Structural Biology, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France; Centre National de la Recherche Scientifique, UMR7104, Illkirch, France; Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France; Université de Strasbourg, Illkirch, France 
2  Research Group ‘Chromosome Organization and Dynamics’, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried, Germany 
3  Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA 
Pages
1-7
Publication year
2017
Publication date
Nov 2017
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-017-01564-7
ProQuest document ID
1983423912
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.