Abstract

Nanodiamonds have many attractive properties that make them suitable for a range of biological applications, but their practical use has been limited because nanodiamond conjugates tend to aggregate in solution during or after functionalisation. Here we demonstrate the production of DNA-detonation nanodiamond (DNA-DND) conjugates with high dispersion and solubility using an ultrasonic, mixed-silanization chemistry protocol based on the in situ Bead-Assisted Sonication Disintegration (BASD) silanization method. We use two silanes to achieve these properties: (1) 3-(trihydroxysilyl)propyl methylphosphonate (THPMP); a negatively charged silane that imparts high zeta potential and solubility in solution; and (2) (3-aminopropyl)triethoxysilane (APTES); a commonly used functional silane that contributes an amino group for subsequent bioconjugation. We target these amino groups for covalent conjugation to thiolated, single-stranded DNA oligomers using the heterobifunctional crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC). The resulting DNA-DND conjugates are the smallest reported to date, as determined by Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM). The functionalisation method we describe is versatile and can be used to produce a wide variety of soluble DND-biomolecule conjugates.

Details

Title
Functionalisation of Detonation Nanodiamond for Monodispersed, Soluble DNA-Nanodiamond Conjugates Using Mixed Silane Bead-Assisted Sonication Disintegration
Author
Edgington, Robert 1 ; Spillane, Katelyn M 2 ; Papageorgiou, George 3 ; Wray, William 4 ; Ishiwata, Hitoshi 5 ; Labarca, Mariana 5 ; Leal-Ortiz, Sergio 5 ; Reid, Gordon 3 ; Webb, Martin 3 ; Foord, John 6 ; Melosh, Nicholas 5 ; Schaefer, Andreas T 4 

 The Francis Crick Institute, London, UK; Department of Materials Science and Engineering, Stanford University, Stanford, California, United States; Department of Chemistry, University of Oxford, Oxford, UK 
 The Francis Crick Institute, London, UK; Department of Physics, King’s College London, London, United Kingdom 
 The Francis Crick Institute, London, UK 
 The Francis Crick Institute, London, UK; Department of Neuroscience, Physiology & Pharmacology, University College London, London, UK 
 Department of Materials Science and Engineering, Stanford University, Stanford, California, United States 
 Department of Chemistry, University of Oxford, Oxford, UK 
Pages
1-11
Publication year
2018
Publication date
Jan 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1987710058
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.