Abstract

This study investigated global gene and protein expression in the small yellow follicle (SYF; 6–8 mm in diameter) tissues of chickens in response to acute heat stress. Twelve 30-week-old layer-type hens were divided into four groups: control hens were maintained at 25 °C while treatment hens were subjected to acute heat stress at 36 °C for 4 h without recovery, with 2-h recovery, and with 6-h recovery. SYFs were collected at each time point for mRNA and protein analyses. A total of 176 genes and 93 distinct proteins with differential expressions were identified, mainly associated with the molecular functions of catalytic activity and binding. The upregulated expression of heat shock proteins and peroxiredoxin family after acute heat stress is suggestive of responsive machineries to protect cells from apoptosis and oxidative insults. In conclusion, both the transcripts and proteins associated with apoptosis, stress response, and antioxidative defense were upregulated in the SYFs of layer-type hens to alleviate the detrimental effects by acute heat stress. However, the genomic regulations of specific cell type in response to acute heat stress of SYFs require further investigation.

Details

Title
Functional genomics study of acute heat stress response in the small yellow follicles of layer-type chickens
Author
Chuen-Yu, Cheng 1 ; Wei-Lin, Tu 1 ; Chao-Jung, Chen 2 ; Hong-Lin, Chan 3 ; Chih-Feng, Chen 4 ; Chen, Hsin-Hsin 1 ; Pin-Chi Tang 5 ; Yen-Pai, Lee 1 ; Chen, Shuen-Ei 4 ; San-Yuan, Huang 6   VIAFID ORCID Logo 

1  Department of Animal Science, National Chung Hsing University, Taichung, Taiwan 
2  Department of Medical Research, Proteomic Core Laboratory, China Medical University Hospital, Taichung, Taiwan; Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan 
3  Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan; Department of Medical Sciences, National Tsing Hua University, Hsinchu, Taiwan 
4  Department of Animal Science, National Chung Hsing University, Taichung, Taiwan; Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan; Center for the Integrative and Evolutionary Galliformes Genomics, iEGG Center, National Chung Hsing University, Taichung, Taiwan 
5  Department of Animal Science, National Chung Hsing University, Taichung, Taiwan; Department of Medical Research, Proteomic Core Laboratory, China Medical University Hospital, Taichung, Taiwan 
6  Department of Animal Science, National Chung Hsing University, Taichung, Taiwan; Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan; Center for the Integrative and Evolutionary Galliformes Genomics, iEGG Center, National Chung Hsing University, Taichung, Taiwan; Research Center for Sustainable Energy and Nanotechnology, National Chung Hsing University, Taichung, Taiwan 
Pages
1-11
Publication year
2018
Publication date
Jan 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-017-18335-5
ProQuest document ID
1989903464
Copyright
© 2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.