Abstract

Hyperbaric oxygen treatment (HBO) promotes rapid recovery from soft tissue injuries. However, the healing mechanism is unclear. Here we assessed the effects of HBO on contused calf muscles in a rat skeletal muscle injury model. An experimental HBO chamber was developed and rats were treated with 100% oxygen, 2.5 atmospheres absolute for 2 h/day after injury. HBO reduced early lower limb volume and muscle wet weight in contused muscles, and promoted muscle isometric strength 7 days after injury. HBO suppressed the elevation of circulating macrophages in the acute phase and then accelerated macrophage invasion into the contused muscle. This environment also increased the number of proliferating and differentiating satellite cells and the amount of regenerated muscle fibers. In the early phase after injury, HBO stimulated the IL-6/STAT3 pathway in contused muscles. Our results demonstrate that HBO has a dual role in decreasing inflammation and accelerating myogenesis in muscle contusion injuries.

Details

Title
Hyperbaric oxygen reduces inflammation, oxygenates injured muscle, and regenerates skeletal muscle via macrophage and satellite cell activation
Author
Oyaizu, Takuya 1 ; Enomoto, Mitsuhiro 2 ; Yamamoto, Naoki 1 ; Tsuji, Kunikazu 3 ; Horie, Masaki 4 ; Muneta, Takeshi 5 ; Sekiya, Ichiro 6   VIAFID ORCID Logo  ; Okawa, Atsushi 7 ; Yagishita, Kazuyoshi 2 

1  Department of Orthopaedic Surgery, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan; Hyperbaric Medical Center, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan 
2  Hyperbaric Medical Center, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan; Sports Medicine Clinical Center, Medical Hospital, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan 
3  Department of Cartilage Regeneration, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan 
4  Hyperbaric Medical Center, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan 
5  Department of Joint Surgery and Sports Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan 
6  Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan 
7  Department of Orthopaedic Surgery, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan 
Pages
1-12
Publication year
2018
Publication date
Jan 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-19670-x
ProQuest document ID
1989903479
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.