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About the Authors:
Guangfei Tang
Contributed equally to this work with: Guangfei Tang, Yun Chen
Roles Data curation, Formal analysis, Investigation, Methodology, Validation
Affiliation: Institute of Biotechnology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China
Yun Chen
Contributed equally to this work with: Guangfei Tang, Yun Chen
Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Validation, Visualization, Writing - original draft
Affiliation: Institute of Biotechnology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China
Jin-Rong Xu
Roles Formal analysis, Resources, Writing - review & editing
Affiliation: Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana, United States of America
H. Corby Kistler
Roles Formal analysis, Funding acquisition, Resources, Writing - review & editing
Affiliation: Department of Plant Pathology, University of Minnesota, St. Paul, Minnesota, United States of America
ORCID http://orcid.org/0000-0001-5312-6297
Zhonghua Ma
Roles Conceptualization, Formal analysis, Funding acquisition, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing
* E-mail: [email protected]
Affiliations Institute of Biotechnology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China, State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, China
ORCID http://orcid.org/0000-0002-5426-0997Abstract
Myosin-I molecular motors are proposed to function as linkers between membranes and the actin cytoskeleton in several cellular processes, but their role in the biosynthesis of fungal secondary metabolites remain elusive. Here, we found that the myosin I of Fusarium graminearum (FgMyo1), the causal agent of Fusarium head blight, plays critical roles in mycotoxin biosynthesis. Inhibition of myosin I by the small molecule phenamacril leads to marked reduction in deoxynivalenol (DON) biosynthesis. FgMyo1 also governs translation of the DON biosynthetic enzyme Tri1 by interacting with the ribosome-associated protein FgAsc1. Disruption of the ATPase activity of FgMyo1 either by the mutation E420K, down-regulation of FgMyo1 expression or deletion of FgAsc1 results in reduced Tri1 translation. The DON biosynthetic enzymes Tri1 and Tri4 are mainly localized to subcellular structures known as toxisomes in response to mycotoxin induction and the FgMyo1-interacting protein, actin, participates in toxisome formation. The actin polymerization disruptor latrunculin A inhibits toxisome assembly. Consistent with this observation, deletion of the actin-associated proteins FgPrk1 and FgEnd3 also results in reduced toxisome formation. Unexpectedly,...