Abstract

Here we report the discovery of two Tupanvirus strains, the longest tailed Mimiviridae members isolated in amoebae. Their genomes are 1.44–1.51 Mb linear double-strand DNA coding for 1276–1425 predicted proteins. Tupanviruses share the same ancestors with mimivirus lineages and these giant viruses present the largest translational apparatus within the known virosphere, with up to 70 tRNA, 20 aaRS, 11 factors for all translation steps, and factors related to tRNA/mRNA maturation and ribosome protein modification. Moreover, two sequences with significant similarity to intronic regions of 18 S rRNA genes are encoded by the tupanviruses and highly expressed. In this translation-associated gene set, only the ribosome is lacking. At high multiplicity of infections, tupanvirus is also cytotoxic and causes a severe shutdown of ribosomal RNA and a progressive degradation of the nucleus in host and non-host cells. The analysis of tupanviruses constitutes a new step toward understanding the evolution of giant viruses.

Details

Title
Tailed giant Tupanvirus possesses the most complete translational apparatus of the known virosphere
Author
Abrahão, Jônatas 1 ; Silva, Lorena 1 ; Ludmila Santos Silva 1 ; Jacques Yaacoub Bou Khalil 2 ; Rodrigues, Rodrigo 3 ; Arantes, Thalita 3 ; Assis, Felipe 3 ; Boratto, Paulo 3 ; Andrade, Miguel 4 ; Kroon, Erna Geessien 3 ; Bergmann Ribeiro 4   VIAFID ORCID Logo  ; Bergier, Ivan 5   VIAFID ORCID Logo  ; Seligmann, Herve 6 ; Ghigo, Eric 6 ; Colson, Philippe 6 ; Levasseur, Anthony 6 ; Kroemer, Guido 7 ; Raoult, Didier 6 ; Bernard La Scola 6 

1  MEPHI, APHM, IRD 198, Aix Marseille Univ, IHU-Méditerranee Infection, Marseille, France; Laboratório de Vírus, Instituto de Ciências Biológicas, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil 
2  CNRS, Marseille, France 
3  Laboratório de Vírus, Instituto de Ciências Biológicas, Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil 
4  Laboratório de Microscopia Eletrônica e Virologia, Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Asa Norte, Brasília, Brazil 
5  Lab. Biomass Conversion, Embrapa Pantanal, Corumbá/MS, Brazil 
6  MEPHI, APHM, IRD 198, Aix Marseille Univ, IHU-Méditerranee Infection, Marseille, France 
7  Cell Biology and Metabolomics Platforms, Gustave Roussy Cancer Campus, Villejuif, France; Equipe 11 labellisée Ligue Nationale contre le Cancer, Centre de Recherche des Cordeliers, Paris, France; Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Université Pierre et Marie Curie, Paris, France; Pôle de Biologie, Hôpital Européen Georges Pompidou, AP-HP, Paris, France; Department of Women’s and Children’s Health, Karolinska University Hospital, Stockholm, Sweden 
Pages
1-12
Publication year
2018
Publication date
Feb 2018
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41467-018-03168-1
ProQuest document ID
2010925259
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.