Abstract

Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development.

Details

Title
Expansion of functional personalized cells with specific transgene combinations
Author
Lipps, Christoph 1 ; Klein, Franziska 2 ; Wahlicht, Tom 3 ; Seiffert, Virginia 2 ; Butueva, Milada 3 ; Zauers, Jeannette 4 ; Truschel, Theresa 4 ; Luckner, Martin 4 ; Köster, Mario 2 ; MacLeod, Roderick 5 ; Pezoldt, Jörn 6   VIAFID ORCID Logo  ; Hühn, Jochen 6 ; Yuan, Qinggong 7 ; Müller, Peter Paul 2 ; Kempf, Henning 8 ; Zweigerdt, Robert 8   VIAFID ORCID Logo  ; Dittrich-Breiholz, Oliver 9 ; Pufe, Thomas 10 ; Beckmann, Rainer 10 ; Drescher, Wolf 11 ; Riancho, Jose 12 ; Sañudo, Carolina 12 ; Korff, Thomas 13 ; Opalka, Bertram 14 ; Rebmann, Vera 15 ; Göthert, Joachim R 14 ; Alves, Paula M 16 ; Ott, Michael 7 ; Schucht, Roland 4 ; Hauser, Hansjörg 2 ; Wirth, Dagmar 17 ; May, Tobias 4   VIAFID ORCID Logo 

 Model Systems for Infection and Immunity, HZI – Helmholtz Centre for Infection Research, Braunschweig, Germany; Experimental Cardiology, Justus-Liebig University Giessen, Giessen, Germany 
 Department of Gene Regulation and Differentiation, HZI – Helmholtz Centre for Infection Research, Braunschweig, Germany 
 Model Systems for Infection and Immunity, HZI – Helmholtz Centre for Infection Research, Braunschweig, Germany 
 InSCREENeX GmbH, Braunschweig, Germany 
 Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany 
 Experimental Immunology, HZI – Helmholtz Centre for Infection Research, Braunschweig, Germany 
 Department of Gastroenterology, Hepatology, Endocrinology, Hannover Medical School, Hannover, Germany; Translational Research Group Cell and Gene Therapy, Twincore - Centre for Experimental and Clinical Infection Research GmbH, Hannover, Germany 
 Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Hannover Medical School, MHH, Hannover, Germany 
 Research Core Unit Genomics, Medical School Hannover, Hannover, Germany 
10  Department of Anatomy and Cell Biology, RWTH Aachen University, Aachen, Germany 
11  Department of Orthopaedics, Aachen University Hospital, RWTH Aachen University, Aachen, Germany; Department of Orthopedic Surgery of the Lower Limb and Arthroplasty, Rummelsberg Hospital, Schwarzenbruck, Germany 
12  Department of Internal Medicine, Hospital U.M. Valdecilla, University of Cantabria, IDIVAL, Santander, Spain 
13  Institute of Physiology and Pathophysiology, RG Blood Vessel Remodeling, University Heidelberg, Heidelberg, Germany 
14  Department of Hematology, West German Cancer Center (WTZ), University Hospital Essen, Essen, Germany 
15  Institute for Transfusion Medicine, University Hospital Essen, Essen, Germany 
16  Instituto de Biologia Experimental e Tecnologica, Universidade Nova de Lisboa, Oeiras, Portugal 
17  Model Systems for Infection and Immunity, HZI – Helmholtz Centre for Infection Research, Braunschweig, Germany; Experimental Hematology, Hannover Medical School, Hannover, Germany 
Pages
1-12
Publication year
2018
Publication date
Mar 2018
Publisher
Nature Publishing Group
e-ISSN
20411723
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2012123409
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.