Abstract

The survival in freezing temperature for woody plants is exclusively dependent on the perception of coldness and induction of dormancy. CBF/DREB1 transcriptional factors join cold-response conduits and the DAM genes, especially PmDAM6, are well-known regulators of dormancy. Despite the immense importance, little is documented on the association between CBF proteins and the complexity of the promoter region in PmDAM6 with the function of bud dormancy in P. mume. Therefore, this study was based on the cloning of PmDAM6 and six PmCBFs to evaluate their integral roles in the process of bud development. The consistency of expressions in either vegetative or reproductive buds provided a negative control from PmCBFs to PmDAM6 during the onset of dormancy. Besides, PmCBF5 could form heteromeric complexes with PmDAM1 and PmDAM6. PmCBF1, PmCBF3, and PmDAM4 recognized the promoter of PmDAM6 by the alternative binding sites. Therefore, the interactions of these genes formulated the base of an obvious model to respond to the coldness and engendered dormancy release. Findings of this study will further help the unveil the genetic control of bud dormancy and its augmentation in P. mume and may offer an explanation for the vernalization.

Details

Title
PmCBFs synthetically affect PmDAM6 by alternative promoter binding and protein complexes towards the dormancy of bud for Prunus mume
Author
Zhao, Kai 1   VIAFID ORCID Logo  ; Zhou, Yuzhen 1   VIAFID ORCID Logo  ; Sagheer Ahmad 1 ; Xue, Yong 1 ; Xie, Xuehua 1 ; Han, Yu 1   VIAFID ORCID Logo  ; Li, Yushu 1 ; Sun, Lidan 1 ; Zhang, Qixiang 2 

1  Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, China 
2  Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, China; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China 
Pages
1-10
Publication year
2018
Publication date
Mar 2018
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1038/s41598-018-22537-w
ProQuest document ID
2013966715
Copyright
© 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.