The effects of crystalloid versus synthetic colloid in vitro on immune cells, co-cultured with mouse splenocytes

Abstract

This study assessed the effects of crystalloid versus synthetic colloid in vitro on immune cells co-cultured with mouse splenocytes. Mouse splenocytes were co-cultured with three different types of fluid: Plasma solution-A^® (CJ HealthCare, Seoul, Korea; the crystalloid group); Tetraspan 6%^® (B. Braun Medical, Melsungen, Germany; the Colloid-T group); and Volulyte 6%^® (Fresenius Kabi, Bad Homburg vor dér-Höhe, Germany; Colloid-V group). To evaluate the acquired immune response, cluster of differentiation (CD) 4⁺ T cells and CD8⁺ T cells were measured. To evaluate the innate immune response, neutrophils were measured. The frequencies of CD4⁺ and CD8⁺ T cells did not differ significantly among the three groups on day 1 or 3. However, the frequencies of CD4⁺ and CD8⁺ T cells in the two synthetic colloid groups were significantly higher than those in the crystalloid group on day 7. On day 1, the frequency of neutrophils was significantly lower in the two synthetic colloid groups, compared with the crystalloid group. However, the values on the other days were similar among all three groups. In conclusion, crystalloid had a limited effect on the immune response; on the other hand, synthetic colloid increased the acquired immune response, although it temporarily inhibited the innate immune response.