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Hypoparathyroidism is a serious health problem that most commonly occurs as a complication of thyroid surgery. Postoperative hypoparathyroidism may be observed in three different variations such as transient (occurs within 3-6 months), protracted (occurs within 1 year) or permanent (occurs after the 1st year) (1). Because parathyroid hormone (PTH) has multiple metabolic functions, control of hypoparathyroidism symptoms with calcium (Ca) replacement therapy can be very difficult (2). Parathyroid allotransplantation for permanent hypoparathyroidism is a relatively new option for treatment (2-4). Upon extensive literature review, it is noted that donors are selected from cadavers or patients with secondary hyperparathyroidism. Primary parathyroid adenomas are the most common cases of surgical intervention indicated in the literature due to hyperparathyroidism, but these patients have not been used as donors thus far (5-7). Cells isolated from adenomatous parathyroid tissue are superior to healthy and secondary hyperplastic cells both in quantity and proliferation rate. However, because adenoma is a benign tumor, there are some concerns about its possible behavior in recipients (5, 6).The potential for functional or histopathologic transformation of adenoma cells is not yet known (5-7). In this study, we aimed to pre-evaluate the usability of this tissue with xenotransplantation from human to rabbit because it has potential to be quite valuable as a cell source.
Material and Methods
This research was performed in Bezmialem Vakif University, and the research protocol was approved by the same university’s Local Animals Ethics Committee. All protocols were in accordance with the regulations governing the care and use of laboratory animals of the declaration of Helsinki.
Seven male New Zealand white rabbits (mean weight, 3700 220 g; 3700±220 g, mean age 4.5 months) without bred production were used. The rabbits were sheltered at one per cage in standard cages, with top and bottom parts made of stainless metal and sides made of woven wire. The floors of the cages were covered with wood shavings, which were changed daily. Rabbits were kept at room temperature and with adequate ventilation. Water and feeding containers were made of standard plastic, with sideways entrances. Animals were fed specially produced pellet feeds for small laboratory animals.
Procedure for Parathyroid Tissue Donor Patient: A 49-year-old male patient was admitted to the outpatient clinic with malignant hypercalcemia (blood calcium level >11...